RLG00000014528

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0022898
Physical Location & Seq
Forward (+)
54371936 .. 54374095
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014528

Sequence Viewer

Length: 459 bp
ATGGGAAATCCATGCAAGGACTGGGCGTTCTTCCAAGTGATCAACCATGGAGTGCCGGTGGATAAGCTCCAAAAGATTGGGGCTGCTACTAGGAAGTTCTTTGCTCGGCCTTTGGAGGAGAAGAGGAAGAGTTGGAGGGATGAAACAAGCGTGATAGGTTACAATGAGAAAGAGAAAACCCAGAATGTCAGAGACCGGAAGGAGGTGTTTAAATTAATAGTGGAAGAGCCTACCTTAGAGGACAAGGAGGAGACAGGAATGGACTACCAATGGCCCCAAGGGCTGTCGACCAATGCCGAAGACCTCTCGAACCTTGACATCATTCTGAAGCAAGCTCATGTCTCTCTACAAGCCGAGTCGATTCGGTTGCTTCCCCTTTTGGACCAGATTGATCCCTCCGTGCATTCGCTTCGATACGTCTACATCCTGGAAGCATGCGCTTTTAAAGGGGCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

153

Amino Acids

17.46

Weight (kDa)

5.67

Isoelectric Point (pI)

27.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 3 - 93 3.4e-19 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022898)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014528
rosa_multiflora Rmu_sc0012608.1_g000001 Rmu_ssc0000050.1_g000029
rosa_samantha Rh6AG104800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 287, 420
AclWI GGATC 1 cut(s) 386
AcuI CTGAAG 1 cut(s) 347
AfiI CCNNNNNNNGG 1 cut(s) 202
AjnI CCWGG 1 cut(s) 426
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 3 cut(s) 67, 335, 456
AluI AGCT 3 cut(s) 67, 335, 456
Alw26I GTCTC 3 cut(s) 186, 245, 346
AlwI GGATC 1 cut(s) 386
AoxI GGCC 2 cut(s) 107, 272
ApeKI GCWGC 1 cut(s) 83
AseI ATTAAT 1 cut(s) 215
AspLEI GCGC 1 cut(s) 440
AspS9I GGNCC 2 cut(s) 273, 382
AvaII GGWCC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 70
BcgI CGANNNNNNTGC 4 cut(s) 349, 383, 392, 426
BciT130I CCWGG 1 cut(s) 428
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 3 cut(s) 186, 245, 346
BfaI CTAG 2 cut(s) 90, 457
BglI GCCNNNNNGGC 1 cut(s) 280
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 273, 382
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 428
BmrI ACTGGG 1 cut(s) 31
BmuI ACTGGG 1 cut(s) 31
BpiI GAAGAC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 46, 277
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 2 cut(s) 46, 277
BseGI GGATG 2 cut(s) 145, 423
BseLI CCNNNNNNNGG 1 cut(s) 202
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 2 cut(s) 131, 263
BseXI GCAGC 1 cut(s) 70
BshFI GGCC 2 cut(s) 109, 274
BsiSI CCGG 2 cut(s) 56, 196
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 3 cut(s) 186, 245, 346
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 2 cut(s) 109, 274
Bso31I GGTCTC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 39, 391
Bsp19I CCATGG 1 cut(s) 46
BspANI GGCC 2 cut(s) 109, 274
BspLI GGNNCC 1 cut(s) 275
BspPI GGATC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 46, 277
BssMI GATC 2 cut(s) 39, 391
BssT1I CCWWGG 2 cut(s) 46, 277
Bst2UI CCWGG 1 cut(s) 428
Bst6I CTCTTC 3 cut(s) 116, 122, 219
BstC8I GCNNGC 3 cut(s) 333, 436, 454
BstDEI CTNAG 1 cut(s) 235
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 2 cut(s) 145, 423
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 2 cut(s) 42, 394
BstMAI GTCTC 3 cut(s) 186, 245, 346
BstMBI GATC 2 cut(s) 39, 391
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 77
BsuRI GGCC 2 cut(s) 109, 274
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 2 cut(s) 145, 423
Cac8I GCNNGC 3 cut(s) 333, 436, 454
CfoI GCGC 1 cut(s) 440
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 273, 382
CviAII CATG 4 cut(s) 12, 47, 338, 435
CviJI RGCY 9 cut(s) 67, 83, 109, 229, 274, 283, 335, 353, 456
CviKI_1 RGCY 9 cut(s) 67, 83, 109, 229, 274, 283, 335, 353, 456
DdeI CTNAG 1 cut(s) 235
DpnI GATC 2 cut(s) 41, 393
DpnII GATC 2 cut(s) 39, 391
DraI TTTAAA 2 cut(s) 211, 445
Eam1104I CTCTTC 3 cut(s) 116, 122, 219
EarI CTCTTC 3 cut(s) 116, 122, 219
Eco130I CCWWGG 2 cut(s) 46, 277
Eco31I GGTCTC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 426
EcoT14I CCWWGG 2 cut(s) 46, 277
ErhI CCWWGG 2 cut(s) 46, 277
FaeI CATG 4 cut(s) 15, 50, 341, 438
FaiI YATR 4 cut(s) 13, 48, 339, 436
FatI CATG 4 cut(s) 11, 46, 337, 434
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 2 cut(s) 287, 420
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 2 cut(s) 152, 410
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 2 cut(s) 90, 457
GlaI GCGC 1 cut(s) 439
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 2 cut(s) 109, 274
HapII CCGG 2 cut(s) 56, 196
HhaI GCGC 1 cut(s) 440
Hin1II CATG 4 cut(s) 15, 50, 341, 438
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HinfI GANTC 2 cut(s) 356, 361
HpaII CCGG 2 cut(s) 56, 196
Hpy166II GTNNAC 2 cut(s) 288, 421
Hpy188I TCNGA 2 cut(s) 191, 327
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 2 cut(s) 288, 421
HpyAV CCTTC 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 2 cut(s) 15, 403
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 1 cut(s) 235
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 4 cut(s) 15, 50, 341, 438
HspAI GCGC 1 cut(s) 438
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 2 cut(s) 39, 391
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 1 cut(s) 72
LpnPI CCDG 8 cut(s) 7, 69, 194, 209, 240, 398, 413, 440
Lsp1109I GCAGC 1 cut(s) 70
MaeI CTAG 2 cut(s) 90, 457
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 1 cut(s) 158
MalI GATC 2 cut(s) 41, 393
MboI GATC 2 cut(s) 39, 391
MboII GAAGA 5 cut(s) 22, 133, 139, 236, 311
MluCI AATT 1 cut(s) 212
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 113
MnlI CCTC 8 cut(s) 109, 117, 129, 196, 232, 241, 314, 406
MseI TTAA 3 cut(s) 210, 215, 444
MspI CCGG 2 cut(s) 56, 196
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 1 cut(s) 280
NcoI CCATGG 1 cut(s) 46
NdeII GATC 2 cut(s) 39, 391
NlaIII CATG 4 cut(s) 15, 50, 341, 438
NlaIV GGNNCC 1 cut(s) 275
NmeAIII GCCGAG 2 cut(s) 85, 379
NspI RCATGY 1 cut(s) 438
PaeI GCATGC 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 219
PctI GAATGC 1 cut(s) 403
PfeI GAWTC 1 cut(s) 361
PfoI TCCNGGA 1 cut(s) 426
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PshBI ATTAAT 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 2 cut(s) 273, 382
SalI GTCGAC 1 cut(s) 286
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 3 cut(s) 210, 215, 444
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 39, 391
Sau96I GGNCC 2 cut(s) 273, 382
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 1 cut(s) 428
SetI ASST 9 cut(s) 69, 160, 207, 236, 306, 315, 337, 420, 458
SinI GGWCC 1 cut(s) 382
SphI GCATGC 1 cut(s) 438
Sse9I AATT 1 cut(s) 212
SspMI CTAG 2 cut(s) 90, 457
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 2 cut(s) 46, 277
TaiI ACGT 1 cut(s) 420
TaqI TCGA 4 cut(s) 287, 308, 359, 412
TasI AATT 1 cut(s) 212
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 3 cut(s) 210, 215, 444
Tru9I TTAA 3 cut(s) 210, 215, 444
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 1 cut(s) 156
TspGWI ACGGA 1 cut(s) 388
VpaK11BI GGWCC 1 cut(s) 382
VspI ATTAAT 1 cut(s) 215
XceI RCATGY 1 cut(s) 438
XcmI CCANNNNNNNNNTGG 1 cut(s) 18
XmiI GTMKAC 2 cut(s) 287, 420
XspI CTAG 2 cut(s) 90, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.