RLG00000016183

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020025
Physical Location & Seq
Reverse (-)
4539470 .. 4540653
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016183

Sequence Viewer

Length: 1143 bp
ATGCCTCTTCCCATTTCTGTAGTGGGTAATTTTCTTCACGGTCGGAGCGATCTGTCTCCACTCCATTGGCATAAGCTGGATATTAACATCGCCTCCGAATTGTGTACAAAGGGTTCAGATATTTCCATCATTCGCAGTAATTTGGAGCTGAGTTATAGAGGTCTTCCGAGCCATCTCAGAAATTGTTTCTTGTACATCAGCATGTTTCCTGAAGGCTACGATATCAGCCTTGAGAGGCTCAAACGATTGTGGATTGCAGAGAATTTCATCGTTGCAGGAAAACCACAGGTCACAGAGGAATGGCATGCAAAAAATTATCTCAATGAACTGGTGGAAAGAAATTTGGTTCCGGTGTGCAATGCGAATGGCATAGATTTAAGGCGGAAGGGATTCCTCACCATTTCCGAGCCAGAATACACACCTGCAGATAAAAGAAGCATTCGATGGCTGTCTGCTTACGAGGGTGGTATTGATTCTCTAAAGGGTTTCATTGGTGGCGTTCGCAGCTTTCTAGGGTCCAAAGAGGCAAGTAATCCTGAGATTGAAATGGTCCTAAAATCAAGTAAGTTTTTAAGGATTTTAGATTTGGAAGGTCTACCTGATTTGAAGGATTTCCCCAAATATGTTTTTCATCTGATTCTGTTAAAATATTTGAGTCTAAGGAAGACCGGTGTTGAAAAAGTTCCCAAGGACATCAGAAAGCTTGTGCTTTTAGAAACCTTCGATCTTAAAGACACTATGGTGACTGAATTGCCTGTAGAGACTAAGAGTCTCTCAAAGTTGCGACATCTTCTAGTCAATACCTCTAATGGCGAAGGAGTAAAGATTTTGGAAGATGTTGGTGGTTCACCTGGTACTCTCCACAAGCTGTCACTGATTAAGGTGCCAAACATAGATTTCATTAAATCCTTGGGAAAACTGAGACTTCTCAAGAAGTTGGGGCTGGAAGACCTTCAAACAACAGATGGTAAAGAATTGTGCAATGTCCTTGAGCAAGTGAAGGGGTTTCTTAACCTTGAATGTAAGAGCGAAAGACAAGAAGTGTCTCGATTTAGATCATTATGCCAGCCCTCCTTTATTTCTCTGGCATCTATGTTTGAGAGGCCCCCTAGCAGGGATACCAAACTGGATCTCCAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

381

Amino Acids

43.02

Weight (kDa)

8.84

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 68 - 117 5.8e-10 Disease resistance protein Winged helix domain
LRR_14 PF23598 167 - 349 4.8e-23 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020025)

Species Orthologous Gene IDs
rosa_laevigata RLG00000016183
rosa_roxburghii Rroxscaffold_2G00150510
rosa_rugosa Rorug02G0013300 Rorug02G0013400
rosa_samantha Rh2AG059500 Rh2BG058200 Rh2CG060000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 1 cut(s) 430
AccB1I GGYRCC 1 cut(s) 883
AccI GTMKAC 1 cut(s) 595
AciI CCGC 1 cut(s) 382
AclWI GGATC 1 cut(s) 1137
AcsI RAATTY 2 cut(s) 262, 340
AcuI CTGAAG 1 cut(s) 231
AfaI GTAC 3 cut(s) 106, 194, 856
AfiI CCNNNNNNNGG 2 cut(s) 1113, 1114
AgeI ACCGGT 1 cut(s) 668
AgsI TTSAA 5 cut(s) 545, 607, 677, 956, 1019
AhdI GACNNNNNGTC 1 cut(s) 768
AjnI CCWGG 1 cut(s) 850
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 6 cut(s) 76, 148, 507, 703, 868, 1139
AluI AGCT 6 cut(s) 76, 148, 507, 703, 868, 1139
Alw26I GTCTC 5 cut(s) 60, 755, 776, 916, 1050
AlwI GGATC 1 cut(s) 1137
AoxI GGCC 1 cut(s) 1103
ApeKI GCWGC 1 cut(s) 504
ApoI RAATTY 2 cut(s) 262, 340
AsiGI ACCGGT 1 cut(s) 668
Asp700I GAANNNNTTC 4 cut(s) 389, 611, 681, 951
AspS9I GGNCC 3 cut(s) 516, 550, 1104
AsuHPI GGTGA 3 cut(s) 388, 754, 840
AvaII GGWCC 2 cut(s) 516, 550
BanI GGYRCC 1 cut(s) 883
BbsI GAAGAC 3 cut(s) 155, 671, 954
BbvI GCAGC 1 cut(s) 516
BccI CCATC 4 cut(s) 134, 180, 438, 959
BciT130I CCWGG 1 cut(s) 852
BciVI GTATCC 1 cut(s) 1111
BcoDI GTCTC 5 cut(s) 60, 755, 776, 916, 1050
BfaI CTAG 3 cut(s) 512, 794, 1110
BfmI CTRYAG 3 cut(s) 18, 423, 756
BfuAI ACCTGC 1 cut(s) 430
BfuI GTATCC 1 cut(s) 1111
BisI GCNGC 1 cut(s) 505
BlsI GCNGC 1 cut(s) 506
Bme1390I CCNGG 1 cut(s) 852
Bme18I GGWCC 2 cut(s) 516, 550
BmeRI GACNNNNNGTC 1 cut(s) 768
BmgT120I GGNCC 3 cut(s) 516, 550, 1104
BmiI GGNNCC 4 cut(s) 348, 517, 885, 1106
BmrFI CCNGG 1 cut(s) 852
BmsI GCATC 1 cut(s) 1097
BpiI GAAGAC 3 cut(s) 155, 671, 954
BpuEI CTTGAG 3 cut(s) 251, 914, 1010
BsaBI GATNNNNATC 1 cut(s) 1054
BsaJI CCNNGG 2 cut(s) 687, 909
BsaWI WCCGGW 2 cut(s) 349, 668
BsaXI ACNNNNNCTCC 3 cut(s) 77, 107, 1116
Bsc4I CCNNNNNNNGG 2 cut(s) 1113, 1114
Bse118I RCCGGY 1 cut(s) 668
Bse1I ACTGG 2 cut(s) 333, 1131
Bse3DI GCAATG 2 cut(s) 364, 988
Bse8I GATNNNNATC 1 cut(s) 1054
BseBI CCWGG 1 cut(s) 852
BseDI CCNNGG 2 cut(s) 687, 909
BseJI GATNNNNATC 1 cut(s) 1054
BseLI CCNNNNNNNGG 2 cut(s) 1113, 1114
BseMI GCAATG 2 cut(s) 364, 988
BseMII CTCAG 4 cut(s) 140, 190, 528, 911
BseNI ACTGG 2 cut(s) 333, 1131
BseXI GCAGC 1 cut(s) 516
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 1 cut(s) 1105
BshNI GGYRCC 1 cut(s) 883
BshTI ACCGGT 1 cut(s) 668
BsiEI CGRYCG 1 cut(s) 43
BsiSI CCGG 2 cut(s) 350, 669
BslI CCNNNNNNNGG 2 cut(s) 1113, 1114
BsmAI GTCTC 5 cut(s) 60, 755, 776, 916, 1050
BsmI GAATGC 1 cut(s) 438
BsnI GGCC 1 cut(s) 1105
Bsp1407I TGTACA 2 cut(s) 104, 192
Bsp143I GATC 4 cut(s) 49, 724, 1055, 1129
BspACI CCGC 1 cut(s) 382
BspANI GGCC 1 cut(s) 1105
BspCNI CTCAG 4 cut(s) 141, 189, 529, 912
BspLI GGNNCC 4 cut(s) 348, 517, 885, 1106
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 1 cut(s) 1137
BspT107I GGYRCC 1 cut(s) 883
BsrDI GCAATG 2 cut(s) 364, 988
BsrFI RCCGGY 1 cut(s) 668
BsrGI TGTACA 2 cut(s) 104, 192
BsrI ACTGG 2 cut(s) 333, 1131
BssAI RCCGGY 1 cut(s) 668
BssECI CCNNGG 2 cut(s) 687, 909
BssMI GATC 4 cut(s) 49, 724, 1055, 1129
BssT1I CCWWGG 2 cut(s) 687, 909
Bst2UI CCWGG 1 cut(s) 852
Bst4CI ACNGT 1 cut(s) 41
Bst6I CTCTTC 1 cut(s) 12
BstAUI TGTACA 2 cut(s) 104, 192
BstC8I GCNNGC 2 cut(s) 306, 1067
BstDEI CTNAG 7 cut(s) 149, 176, 537, 659, 765, 920, 1140
BstKTI GATC 4 cut(s) 52, 727, 1058, 1132
BstMAI GTCTC 5 cut(s) 60, 755, 776, 916, 1050
BstMBI GATC 4 cut(s) 49, 724, 1055, 1129
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 1 cut(s) 504
BstNI CCWGG 1 cut(s) 852
BstNSI RCATGY 2 cut(s) 205, 308
BstSCI CCNGG 1 cut(s) 850
BstSFI CTRYAG 3 cut(s) 18, 423, 756
BstV1I GCAGC 1 cut(s) 516
BstV2I GAAGAC 3 cut(s) 155, 671, 954
BstX2I RGATCY 1 cut(s) 1129
BstXI CCANNNNNNTGG 1 cut(s) 66
BstYI RGATCY 1 cut(s) 1129
BsuI GTATCC 1 cut(s) 1111
BsuRI GGCC 1 cut(s) 1105
BtgZI GCGATG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 872
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 2 cut(s) 306, 1067
Cfr10I RCCGGY 1 cut(s) 668
Cfr13I GGNCC 3 cut(s) 516, 550, 1104
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 3 cut(s) 105, 193, 855
CspAI ACCGGT 1 cut(s) 668
CviAII CATG 2 cut(s) 202, 305
CviQI GTAC 3 cut(s) 105, 193, 855
DdeI CTNAG 7 cut(s) 149, 176, 537, 659, 765, 920, 1140
DpnI GATC 4 cut(s) 51, 726, 1057, 1131
DpnII GATC 4 cut(s) 49, 724, 1055, 1129
DriI GACNNNNNGTC 1 cut(s) 768
Eam1104I CTCTTC 1 cut(s) 12
Eam1105I GACNNNNNGTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 687, 909
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 516, 550
Eco57I CTGAAG 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 1104
EcoRII CCWGG 1 cut(s) 850
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 687, 909
ErhI CCWWGG 2 cut(s) 687, 909
FaeI CATG 2 cut(s) 205, 308
FatI CATG 2 cut(s) 201, 304
FblI GTMKAC 1 cut(s) 595
Fnu4HI GCNGC 1 cut(s) 505
Fsp4HI GCNGC 1 cut(s) 505
FspBI CTAG 3 cut(s) 512, 794, 1110
GluI GCNGC 1 cut(s) 505
HaeIII GGCC 1 cut(s) 1105
HapII CCGG 2 cut(s) 350, 669
Hin1II CATG 2 cut(s) 205, 308
HindIII AAGCTT 2 cut(s) 701, 1137
HinfI GANTC 5 cut(s) 390, 473, 637, 655, 769
HpaII CCGG 2 cut(s) 350, 669
HphI GGTGA 3 cut(s) 388, 754, 840
Hpy166II GTNNAC 3 cut(s) 105, 596, 848
Hpy188I TCNGA 8 cut(s) 45, 97, 118, 168, 179, 406, 636, 698
Hpy188III TCNNGA 4 cut(s) 209, 536, 931, 1047
Hpy8I GTNNAC 3 cut(s) 105, 596, 848
HpyAV CCTTC 8 cut(s) 206, 379, 584, 601, 730, 809, 962, 994
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4V TGCA 6 cut(s) 257, 275, 308, 357, 425, 981
HpyF10VI GCNNNNNNNGC 1 cut(s) 504
HpyF3I CTNAG 7 cut(s) 149, 176, 537, 659, 765, 920, 1140
Hsp92II CATG 2 cut(s) 205, 308
Kzo9I GATC 4 cut(s) 49, 724, 1055, 1129
LmnI GCTCC 2 cut(s) 45, 145
Lsp1109I GCAGC 1 cut(s) 516
LweI GCATC 1 cut(s) 1097
MabI ACCWGGT 1 cut(s) 850
MaeI CTAG 3 cut(s) 512, 794, 1110
MaeIII GTNAC 3 cut(s) 289, 742, 870
MalI GATC 4 cut(s) 51, 726, 1057, 1131
MboI GATC 4 cut(s) 49, 724, 1055, 1129
MboII GAAGA 6 cut(s) 26, 155, 676, 782, 845, 959
MflI RGATCY 1 cut(s) 1129
MluCI AATT 9 cut(s) 28, 98, 139, 181, 262, 313, 340, 749, 974
MlyI GAGTC 2 cut(s) 664, 778
MmeI TCCRAC 1 cut(s) 23
MroXI GAANNNNTTC 4 cut(s) 389, 611, 681, 951
MseI TTAA 8 cut(s) 84, 377, 572, 644, 729, 879, 903, 1011
MslI CAYNNNNRTG 2 cut(s) 200, 740
MspI CCGG 2 cut(s) 350, 669
MspR9I CCNGG 1 cut(s) 852
Mva1269I GAATGC 1 cut(s) 438
MvaI CCWGG 1 cut(s) 852
MwoI GCNNNNNNNGC 1 cut(s) 504
NdeII GATC 4 cut(s) 49, 724, 1055, 1129
NlaIII CATG 2 cut(s) 205, 308
NlaIV GGNNCC 4 cut(s) 348, 517, 885, 1106
NmuCI GTSAC 3 cut(s) 289, 742, 870
NspI RCATGY 2 cut(s) 205, 308
OliI CACNNNNGTG 1 cut(s) 740
PaeI GCATGC 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 438
PdmI GAANNNNTTC 4 cut(s) 389, 611, 681, 951
PfeI GAWTC 3 cut(s) 390, 473, 637
PinAI ACCGGT 1 cut(s) 668
PkrI GCNGC 1 cut(s) 506
PleI GAGTC 2 cut(s) 663, 777
PpsI GAGTC 2 cut(s) 663, 777
Psp6I CCWGG 1 cut(s) 850
PspGI CCWGG 1 cut(s) 850
PspN4I GGNNCC 4 cut(s) 348, 517, 885, 1106
PspPI GGNCC 3 cut(s) 516, 550, 1104
PsrI GAACNNNNNNTAC 2 cut(s) 97, 129
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 1129
RsaI GTAC 3 cut(s) 106, 194, 856
RsaNI GTAC 3 cut(s) 105, 193, 855
RseI CAYNNNNRTG 2 cut(s) 200, 740
SaqAI TTAA 8 cut(s) 84, 377, 572, 644, 729, 879, 903, 1011
SatI GCNGC 1 cut(s) 505
Sau3AI GATC 4 cut(s) 49, 724, 1055, 1129
Sau96I GGNCC 3 cut(s) 516, 550, 1104
SchI GAGTC 2 cut(s) 664, 778
ScrFI CCNGG 1 cut(s) 852
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 1 cut(s) 1097
SfcI CTRYAG 3 cut(s) 18, 423, 756
SinI GGWCC 2 cut(s) 516, 550
SmiMI CAYNNNNRTG 2 cut(s) 200, 740
SmlI CTYRAG 3 cut(s) 230, 929, 989
SmoI CTYRAG 3 cut(s) 230, 929, 989
SphI GCATGC 1 cut(s) 308
Sse9I AATT 9 cut(s) 28, 98, 139, 181, 262, 313, 340, 749, 974
SsiI CCGC 1 cut(s) 382
SspI AATATT 1 cut(s) 650
SspMI CTAG 3 cut(s) 512, 794, 1110
StyD4I CCNGG 1 cut(s) 850
StyI CCWWGG 2 cut(s) 687, 909
TaaI ACNGT 1 cut(s) 41
TaqI TCGA 3 cut(s) 442, 723, 1048
TasI AATT 9 cut(s) 28, 98, 139, 181, 262, 313, 340, 749, 974
TatI WGTACW 2 cut(s) 104, 192
TfiI GAWTC 3 cut(s) 390, 473, 637
Tru1I TTAA 8 cut(s) 84, 377, 572, 644, 729, 879, 903, 1011
Tru9I TTAA 8 cut(s) 84, 377, 572, 644, 729, 879, 903, 1011
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 3 cut(s) 289, 742, 870
TseI GCWGC 1 cut(s) 504
Tsp45I GTSAC 3 cut(s) 289, 742, 870
TspDTI ATGAA 5 cut(s) 256, 339, 478, 620, 889
TspRI CASTG 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 516, 550
XapI RAATTY 2 cut(s) 262, 340
XceI RCATGY 2 cut(s) 205, 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 595
XmnI GAANNNNTTC 4 cut(s) 389, 611, 681, 951
XspI CTAG 3 cut(s) 512, 794, 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.