Rh2AG059500

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0020025
Physical Location & Seq
Reverse (-)
4780658 .. 4781221
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG059500.1

Sequence Viewer

Length: 564 bp
ATGGCTAGTGCCGCCATTAACGTTGCATTAAACGCATTTCAGGCTGTCCTACCTGACTTATTGGGTTCAGGCGAAGTGTTAACAAATGCCCAGGATTGGTTGGAGAGGATGAAAGCTATTCTAGAGGATTCAGAAGATGAGGTTGAAGAAGGTAACAAGCAATTTATATCTTATGTGAACGAGGTCGAGAATATAGCTGTTTCTGTTGGGAATGTTCTTGACAGCTTTTTGACTCGCGTGACTCGTCATCGACATCAACCAAACAGATTCGTGCAGAGGAAGATAGCAACCGTCTTACTGGGTAGTAAAATTAGATCTATTCAGACTAGTAGAATTGATCATCTTCCAGCATTCCCGCCAGCAAGTTCCAGCTCTAGCCGTTGGAGCTACAAAGATGTGCAAAATCTTACAGAATGTGATGGATTAGTGGGGTTTGAGAAGGTCGAAGAACAACTTTTTAATAAGTTGCAGAAAGCAAGTCTAGAACGCTCATTTGTGTTGGTTGCAGGTCCTGCTGGTTCTAGGAAAACCGCTCTGGTATATAAATTGTACCAAAACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

187

Amino Acids

20.84

Weight (kDa)

5.96

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 21 - 90 2.8e-07 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020025)

Species Orthologous Gene IDs
rosa_laevigata RLG00000016183
rosa_roxburghii Rroxscaffold_2G00150510
rosa_rugosa Rorug02G0013300 Rorug02G0013400
rosa_samantha Rh2AG059500 Rh2BG058200 Rh2CG060000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 497
AccBSI CCGCTC 1 cut(s) 533
AccII CGCG 1 cut(s) 237
AciI CCGC 3 cut(s) 12, 356, 531
AclI AACGTT 1 cut(s) 21
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 1 cut(s) 146
AhlI ACTAGT 1 cut(s) 326
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 5 cut(s) 116, 197, 225, 372, 387
AluI AGCT 5 cut(s) 116, 197, 225, 372, 387
AlwNI CAGNNNCTG 1 cut(s) 512
AspS9I GGNCC 1 cut(s) 509
AvaII GGWCC 1 cut(s) 509
BccI CCATC 1 cut(s) 413
BceAI ACGGC 1 cut(s) 363
BciT130I CCWGG 1 cut(s) 92
BclI TGATCA 1 cut(s) 337
BcuI ACTAGT 1 cut(s) 326
BfaI CTAG 7 cut(s) 6, 122, 327, 375, 482, 522, 562
BfuAI ACCTGC 1 cut(s) 497
BglII AGATCT 1 cut(s) 314
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 509
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 96
BseNI ACTGG 1 cut(s) 303
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 96
BsmI GAATGC 1 cut(s) 350
Bsp143I GATC 2 cut(s) 314, 337
BspACI CCGC 3 cut(s) 12, 356, 531
BspFNI CGCG 1 cut(s) 237
BspMI ACCTGC 1 cut(s) 497
BsrBI CCGCTC 1 cut(s) 533
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 2 cut(s) 314, 337
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 292
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 360
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 237
BstKTI GATC 2 cut(s) 317, 340
BstMBI GATC 2 cut(s) 314, 337
BstMWI GCNNNNNNNGC 5 cut(s) 11, 32, 41, 384, 512
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstUI CGCG 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 314
BstYI RGATCY 1 cut(s) 314
BtsCI GGATG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 360
CaiI CAGNNNCTG 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 550
CviJI RGCY 8 cut(s) 5, 44, 116, 197, 225, 372, 378, 387
CviKI_1 RGCY 8 cut(s) 5, 44, 116, 197, 225, 372, 378, 387
CviQI GTAC 1 cut(s) 550
DpnI GATC 2 cut(s) 316, 339
DpnII GATC 2 cut(s) 314, 337
Eco47I GGWCC 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 90
FaiI YATR 5 cut(s) 167, 174, 194, 541, 543
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FauI CCCGC 1 cut(s) 363
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 7 cut(s) 6, 122, 327, 375, 482, 522, 562
GluI GCNGC 1 cut(s) 12
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 4 cut(s) 128, 232, 241, 267
HpaI GTTAAC 1 cut(s) 81
Hpy166II GTNNAC 2 cut(s) 81, 178
Hpy188I TCNGA 2 cut(s) 133, 324
Hpy188III TCNNGA 4 cut(s) 122, 187, 218, 482
Hpy8I GTNNAC 2 cut(s) 81, 178
HpyAV CCTTC 2 cut(s) 143, 433
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 5 cut(s) 26, 274, 400, 469, 506
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 32, 41, 384, 512
HpySE526I ACGT 1 cut(s) 21
Ksp22I TGATCA 1 cut(s) 337
KspAI GTTAAC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 314, 337
LmnI GCTCC 1 cut(s) 384
MaeI CTAG 7 cut(s) 6, 122, 327, 375, 482, 522, 562
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 152, 238
MalI GATC 2 cut(s) 316, 339
MbiI CCGCTC 1 cut(s) 533
MboI GATC 2 cut(s) 314, 337
MboII GAAGA 5 cut(s) 146, 158, 292, 335, 458
MflI RGATCY 1 cut(s) 314
MluCI AATT 4 cut(s) 161, 309, 333, 545
MlyI GAGTC 2 cut(s) 226, 235
MmeI TCCRAC 2 cut(s) 81, 362
MnlI CCTC 5 cut(s) 99, 118, 133, 175, 270
MseI TTAA 4 cut(s) 18, 29, 80, 459
MspR9I CCNGG 1 cut(s) 92
Mva1269I GAATGC 1 cut(s) 350
MvaI CCWGG 1 cut(s) 92
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 5 cut(s) 11, 32, 41, 384, 512
NdeII GATC 2 cut(s) 314, 337
NmuCI GTSAC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PctI GAATGC 1 cut(s) 350
PfeI GAWTC 2 cut(s) 128, 267
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 2 cut(s) 226, 235
PpsI GAGTC 2 cut(s) 226, 235
PpuMI RGGWCCY 1 cut(s) 509
Psp1406I AACGTT 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspPI GGNCC 1 cut(s) 509
PspPPI RGGWCCY 1 cut(s) 509
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 314
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
SaqAI TTAA 4 cut(s) 18, 29, 80, 459
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 2 cut(s) 314, 337
Sau96I GGNCC 1 cut(s) 509
SchI GAGTC 2 cut(s) 226, 235
ScrFI CCNGG 1 cut(s) 92
SinI GGWCC 1 cut(s) 509
SpeI ACTAGT 1 cut(s) 326
Sse9I AATT 4 cut(s) 161, 309, 333, 545
SsiI CCGC 3 cut(s) 12, 356, 531
SspMI CTAG 7 cut(s) 6, 122, 327, 375, 482, 522, 562
StyD4I CCNGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 24
TaqI TCGA 3 cut(s) 186, 250, 444
TasI AATT 4 cut(s) 161, 309, 333, 545
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 128, 267
Tru1I TTAA 4 cut(s) 18, 29, 80, 459
Tru9I TTAA 4 cut(s) 18, 29, 80, 459
TseFI GTSAC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 509
XbaI TCTAGA 2 cut(s) 121, 481
XspI CTAG 7 cut(s) 6, 122, 327, 375, 482, 522, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.