Rh2CG060000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0020025
Physical Location & Seq
Reverse (-)
4874770 .. 4875462
693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG060000.1

Sequence Viewer

Length: 693 bp
ATGGCTGGTGCCTCCATTAACGTTGCATTAAACGCATTTCAGGCTGTCCTACCTGACGTATTGGGTTCAGGCGAAGTGTTAACAAATGCCCGAGATTGGTTGGAGAGGATGAAAGCTATTCTAGAGGATTCAGAAGATGAGGTTGAAGAAGGTAACAAGCAATTTATATCTTATGTGAAAGAGGTCGAGAATATAGCTGTTTCTATTGGGAATGTTCTTGACAGCTTTTTGGCTCGCGTGACTCGTCATCGATATCAACCAAACAGATTCGTGCAGAGGAAGATAGCAACCGTCTTACTGGGTAGTAAAATTAGATCTATTCAGACTAATAGAATTGATCATCTTCCAGCATTCCCGCCAACAAGTTCCAGCTCTAGCCGTTGGAGCTACAAAGATGTGCAAAATCTTATAGAATGTGATGGATTAGTGGGGTTTGAGAAGGTCGAAGAACAGCTTTTTAATGAGTTGCAGAAAGCAAGTCTAGAACGCTCATTTGTGTTGGTTGCAGGTCCCGCTGGTTCTGGGAAAACCGCTCTGGTATATAAATTGTACCAAAACGACGAGAGGGTTAGAGCCTTTAGTGTCCGTGAATGGCTTCAAGCCTCAGAGACGCTGGAATCAAGCCTTCAAGGAGAGAAGTTTCTAGTTGTTTTAGATGATGTTTGGAGCAAAGATTGTCGAATATATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

230

Amino Acids

25.92

Weight (kDa)

5.27

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 26 - 90 1.6e-06 Rx N-terminal domain
NB-ARC PF00931 146 - 206 4.6e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020025)

Species Orthologous Gene IDs
rosa_laevigata RLG00000016183
rosa_roxburghii Rroxscaffold_2G00150510
rosa_rugosa Rorug02G0013300 Rorug02G0013400
rosa_samantha Rh2AG059500 Rh2BG058200 Rh2CG060000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 691
Acc36I ACCTGC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 8
AccBSI CCGCTC 1 cut(s) 533
AccII CGCG 1 cut(s) 237
AciI CCGC 3 cut(s) 356, 513, 531
AclI AACGTT 1 cut(s) 21
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 3 cut(s) 146, 599, 629
AluBI AGCT 6 cut(s) 116, 197, 225, 372, 387, 454
AluI AGCT 6 cut(s) 116, 197, 225, 372, 387, 454
Alw26I GTCTC 1 cut(s) 602
Ama87I CYCGRG 1 cut(s) 90
Asp700I GAANNNNTTC 1 cut(s) 594
AspS9I GGNCC 1 cut(s) 509
AvaI CYCGRG 1 cut(s) 90
AvaII GGWCC 1 cut(s) 509
BanI GGYRCC 1 cut(s) 8
BccI CCATC 1 cut(s) 413
BceAI ACGGC 1 cut(s) 363
BclI TGATCA 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 4 cut(s) 122, 375, 482, 644
BfuAI ACCTGC 1 cut(s) 497
BglII AGATCT 1 cut(s) 314
Bme18I GGWCC 1 cut(s) 509
BmeT110I CYCGRG 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 509
BmiI GGNNCC 2 cut(s) 10, 511
BmrI ACTGGG 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 303
BseCI ATCGAT 1 cut(s) 250
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 618
BseNI ACTGG 1 cut(s) 303
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 237
BshNI GGYRCC 1 cut(s) 8
BshVI ATCGAT 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 1 cut(s) 602
BsmBI CGTCTC 1 cut(s) 602
BsmFI GGGAC 1 cut(s) 495
BsmI GAATGC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 90
Bsp143I GATC 2 cut(s) 314, 337
BspACI CCGC 3 cut(s) 356, 513, 531
BspCNI CTCAG 1 cut(s) 617
BspDI ATCGAT 1 cut(s) 250
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 10, 511
BspMI ACCTGC 1 cut(s) 497
BspT107I GGYRCC 1 cut(s) 8
BsrBI CCGCTC 1 cut(s) 533
BsrI ACTGG 1 cut(s) 303
BssMI GATC 2 cut(s) 314, 337
Bst4CI ACNGT 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 1 cut(s) 604
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 237
BstKTI GATC 2 cut(s) 317, 340
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 2 cut(s) 314, 337
BstMWI GCNNNNNNNGC 4 cut(s) 32, 41, 384, 512
BstUI CGCG 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 314
BstYI RGATCY 1 cut(s) 314
Bsu15I ATCGAT 1 cut(s) 250
BsuTUI ATCGAT 1 cut(s) 250
BtsCI GGATG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 235
Cfr13I GGNCC 1 cut(s) 509
ClaI ATCGAT 1 cut(s) 250
CseI GACGC 1 cut(s) 619
Csp6I GTAC 1 cut(s) 550
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 1 cut(s) 604
DpnI GATC 2 cut(s) 316, 339
DpnII GATC 2 cut(s) 314, 337
Eco32I GATATC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 509
Eco88I CYCGRG 1 cut(s) 90
EcoO109I RGGNCCY 1 cut(s) 509
EcoRV GATATC 1 cut(s) 254
Esp3I CGTCTC 1 cut(s) 602
FaiI YATR 8 cut(s) 167, 174, 194, 410, 541, 543, 685, 691
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 1 cut(s) 495
FauI CCCGC 2 cut(s) 363, 520
FbaI TGATCA 1 cut(s) 337
FokI GGATG 1 cut(s) 121
FspBI CTAG 4 cut(s) 122, 375, 482, 644
HgaI GACGC 1 cut(s) 619
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 4 cut(s) 128, 241, 267, 617
HpaI GTTAAC 1 cut(s) 81
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 3 cut(s) 133, 324, 607
Hpy188III TCNNGA 4 cut(s) 122, 187, 218, 482
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 1 cut(s) 563
HpyAV CCTTC 3 cut(s) 143, 433, 635
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 2 cut(s) 21, 57
HpyCH4V TGCA 5 cut(s) 26, 274, 400, 469, 506
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 41, 384, 512
HpyF3I CTNAG 1 cut(s) 604
HpySE526I ACGT 2 cut(s) 21, 57
Ksp22I TGATCA 1 cut(s) 337
KspAI GTTAAC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 314, 337
LmnI GCTCC 2 cut(s) 384, 666
MaeI CTAG 4 cut(s) 122, 375, 482, 644
MaeII ACGT 2 cut(s) 21, 57
MaeIII GTNAC 2 cut(s) 152, 238
MalI GATC 2 cut(s) 316, 339
MbiI CCGCTC 1 cut(s) 533
MboI GATC 2 cut(s) 314, 337
MboII GAAGA 5 cut(s) 146, 158, 292, 335, 458
MflI RGATCY 1 cut(s) 314
MluCI AATT 4 cut(s) 161, 309, 333, 545
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 2 cut(s) 81, 362
MnlI CCTC 8 cut(s) 22, 99, 118, 133, 175, 270, 558, 613
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 4 cut(s) 18, 29, 80, 459
MspA1I CMGCKG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 350
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 4 cut(s) 32, 41, 384, 512
NdeII GATC 2 cut(s) 314, 337
NlaIV GGNNCC 2 cut(s) 10, 511
NmuCI GTSAC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PctI GAATGC 1 cut(s) 350
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 3 cut(s) 128, 267, 617
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
PpuMI RGGWCCY 1 cut(s) 509
PsiI TTATAA 1 cut(s) 691
Psp1406I AACGTT 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 509
PspN4I GGNNCC 2 cut(s) 10, 511
PspPI GGNCC 1 cut(s) 509
PspPPI RGGWCCY 1 cut(s) 509
PsuI RGATCY 1 cut(s) 314
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
SaqAI TTAA 4 cut(s) 18, 29, 80, 459
Sau3AI GATC 2 cut(s) 314, 337
Sau96I GGNCC 1 cut(s) 509
SchI GAGTC 1 cut(s) 235
SinI GGWCC 1 cut(s) 509
Sse9I AATT 4 cut(s) 161, 309, 333, 545
SsiI CCGC 3 cut(s) 356, 513, 531
SspMI CTAG 4 cut(s) 122, 375, 482, 644
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 2 cut(s) 24, 60
TaqI TCGA 4 cut(s) 186, 250, 444, 679
TasI AATT 4 cut(s) 161, 309, 333, 545
TfiI GAWTC 3 cut(s) 128, 267, 617
Tru1I TTAA 4 cut(s) 18, 29, 80, 459
Tru9I TTAA 4 cut(s) 18, 29, 80, 459
TseFI GTSAC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 575
VpaK11BI GGWCC 1 cut(s) 509
XbaI TCTAGA 2 cut(s) 121, 481
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 4 cut(s) 122, 375, 482, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.