RLG00000017292

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020657
Physical Location & Seq
Forward (+)
15036032 .. 15036766
735 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017292

Sequence Viewer

Length: 528 bp
ATGTCAGGGGTTGAGGGGCTTATTGCAGGGGGTGCTATAAGTTCGCCATTTAATGCGCTGTATGATGTCCTTAAGGGAGTGGTGAACAACGTGAGGATGTTTAGAGGCGAGCTCGAAGCTCTCCGTTCGAAGATAAACCTTCTCGGACCTCTGATCGCTCAGATAGAGGAGTTAGACAAGCGGTTGGGCCGTGACCAGAAGGAAGCTAAAGAGCTACTAGAAATTTCAGAAAAAATGAACAAGGGGAAAGAGCTCGTTGGCAAGTGCTCCAAGGTTAGCTGGTGGAATATCCTGAAGAAGAATTCATATACCAACAAGCTTGTGGAATTGGATGGCTCCTTCCAAAGACTCTCGGAAATACTGAAACTGCAAGGATTGGTGGTTGGGAAGGAGACCTTGGTTACAGCAAAGAAGATAGAAGCCGGGATGGATGTGATGGGGTGTTTGATCGATGTAATTCTCCGGAGCTACGTGGGGGGCCAATGGCCGTCCATCTCCATATCCGCCGTGGCACTTCCAGTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

176

Amino Acids

19.17

Weight (kDa)

9.04

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 139 3.6e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020657)

Species Orthologous Gene IDs
rosa_laevigata RLG00000017292 RLG00000017310
rosa_multiflora Rmu_ssc0000257.1_g000001
rosa_roxburghii Rroxscaffold_2G00139080
rosa_samantha Rh2AG169300 Rh2DG174700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 462
AciI CCGC 2 cut(s) 181, 504
AcoI YGGCCR 1 cut(s) 485
AcsI RAATTY 2 cut(s) 222, 301
AcuI CTGAAG 1 cut(s) 314
AflII CTTAAG 1 cut(s) 71
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 8 cut(s) 112, 119, 206, 214, 253, 279, 319, 468
AluI AGCT 8 cut(s) 112, 119, 206, 214, 253, 279, 319, 468
Alw21I GWGCWC 3 cut(s) 114, 255, 269
Alw26I GTCTC 1 cut(s) 386
Aor13HI TCCGGA 1 cut(s) 462
AoxI GGCC 3 cut(s) 187, 478, 485
ApoI RAATTY 2 cut(s) 222, 301
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 3 cut(s) 146, 187, 478
AsuC2I CCSGG 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 128
AvaII GGWCC 1 cut(s) 146
BanII GRGCYC 2 cut(s) 114, 255
Bbv12I GWGCWC 3 cut(s) 114, 255, 269
BccI CCATC 4 cut(s) 326, 421, 430, 500
BceAI ACGGC 3 cut(s) 174, 472, 491
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 1 cut(s) 218
BfrI CTTAAG 1 cut(s) 71
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 3 cut(s) 146, 187, 478
BmiI GGNNCC 2 cut(s) 337, 479
BmrFI CCNGG 1 cut(s) 424
BplI GAGNNNNNCTC 2 cut(s) 96, 128
Bpu14I TTCGAA 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 424
Bsa29I ATCGAT 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 472
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 270, 396, 507
BsaWI WCCGGW 1 cut(s) 462
Bse1I ACTGG 1 cut(s) 518
BseAI TCCGGA 1 cut(s) 462
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 3 cut(s) 270, 396, 507
BseGI GGATG 4 cut(s) 102, 337, 432, 436
BseMII CTCAG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 1 cut(s) 182
BshFI GGCC 3 cut(s) 189, 480, 487
BshVI ATCGAT 1 cut(s) 450
BsiHKAI GWGCWC 3 cut(s) 114, 255, 269
BsiSI CCGG 2 cut(s) 423, 463
BsmAI GTCTC 1 cut(s) 386
BsnI GGCC 3 cut(s) 189, 480, 487
Bso31I GGTCTC 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 3 cut(s) 114, 255, 269
Bsp13I TCCGGA 1 cut(s) 462
Bsp143I GATC 2 cut(s) 153, 447
BspACI CCGC 2 cut(s) 181, 504
BspANI GGCC 3 cut(s) 189, 480, 487
BspCNI CTCAG 1 cut(s) 172
BspDI ATCGAT 1 cut(s) 450
BspEI TCCGGA 1 cut(s) 462
BspLI GGNNCC 2 cut(s) 337, 479
BspT104I TTCGAA 1 cut(s) 128
BspTI CTTAAG 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 386
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 3 cut(s) 270, 396, 507
BssMI GATC 2 cut(s) 153, 447
BssT1I CCWWGG 2 cut(s) 270, 396
BstAFI CTTAAG 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 472
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 1 cut(s) 159
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 4 cut(s) 102, 337, 432, 436
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 2 cut(s) 156, 450
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 2 cut(s) 153, 447
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 450
BsuRI GGCC 3 cut(s) 189, 480, 487
BsuTUI ATCGAT 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 4 cut(s) 102, 337, 432, 436
Cac8I GCNNGC 1 cut(s) 110
CfoI GCGC 1 cut(s) 58
Cfr13I GGNCC 3 cut(s) 146, 187, 478
ClaI ATCGAT 1 cut(s) 450
DdeI CTNAG 1 cut(s) 159
DpnI GATC 2 cut(s) 155, 449
DpnII GATC 2 cut(s) 153, 447
EaeI YGGCCR 1 cut(s) 485
EciI GGCGGA 1 cut(s) 493
Ecl136II GAGCTC 2 cut(s) 112, 253
Eco130I CCWWGG 2 cut(s) 270, 396
Eco24I GRGCYC 2 cut(s) 114, 255
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 2 cut(s) 112, 253
Eco57I CTGAAG 1 cut(s) 314
EcoICRI GAGCTC 2 cut(s) 112, 253
EcoRI GAATTC 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 270, 396
EcoT38I GRGCYC 2 cut(s) 114, 255
ErhI CCWWGG 2 cut(s) 270, 396
FaiI YATR 5 cut(s) 38, 63, 307, 309, 500
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FokI GGATG 4 cut(s) 109, 344, 439, 443
FriOI GRGCYC 2 cut(s) 114, 255
FspBI CTAG 1 cut(s) 218
GlaI GCGC 1 cut(s) 57
HaeIII GGCC 3 cut(s) 189, 480, 487
HapII CCGG 2 cut(s) 423, 463
HhaI GCGC 1 cut(s) 58
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 1 cut(s) 348
HpaII CCGG 2 cut(s) 423, 463
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 5 cut(s) 146, 153, 162, 229, 355
Hpy188III TCNNGA 2 cut(s) 292, 463
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 149, 193, 349, 382
HpyCH4IV ACGT 2 cut(s) 90, 471
HpyCH4V TGCA 2 cut(s) 26, 370
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 2 cut(s) 90, 471
HspAI GCGC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 462
Kzo9I GATC 2 cut(s) 153, 447
LmnI GCTCC 3 cut(s) 272, 341, 465
LpnPI CCDG 6 cut(s) 12, 209, 265, 305, 436, 476
MaeI CTAG 1 cut(s) 218
MaeII ACGT 2 cut(s) 90, 471
MaeIII GTNAC 2 cut(s) 191, 400
MalI GATC 2 cut(s) 155, 449
MboI GATC 2 cut(s) 153, 447
MboII GAAGA 4 cut(s) 142, 307, 310, 424
MhlI GDGCHC 3 cut(s) 114, 255, 269
MluCI AATT 4 cut(s) 222, 301, 326, 456
MlyI GAGTC 1 cut(s) 342
MnlI CCTC 5 cut(s) 7, 87, 98, 159, 160
MroI TCCGGA 1 cut(s) 462
MseI TTAA 2 cut(s) 51, 72
MspCI CTTAAG 1 cut(s) 71
MspI CCGG 2 cut(s) 423, 463
MspR9I CCNGG 1 cut(s) 424
MwoI GCNNNNNNNGC 1 cut(s) 32
NciI CCSGG 1 cut(s) 424
NdeII GATC 2 cut(s) 153, 447
NlaIV GGNNCC 2 cut(s) 337, 479
NmuCI GTSAC 1 cut(s) 191
NspV TTCGAA 1 cut(s) 128
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
Ppu21I YACGTR 1 cut(s) 472
Psp124BI GAGCTC 2 cut(s) 114, 255
PspN4I GGNNCC 2 cut(s) 337, 479
PspPI GGNCC 3 cut(s) 146, 187, 478
SacI GAGCTC 2 cut(s) 114, 255
SaqAI TTAA 2 cut(s) 51, 72
Sau3AI GATC 2 cut(s) 153, 447
Sau96I GGNCC 3 cut(s) 146, 187, 478
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 1 cut(s) 424
SduI GDGCHC 3 cut(s) 114, 255, 269
SfuI TTCGAA 1 cut(s) 128
SinI GGWCC 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 4 cut(s) 222, 301, 326, 456
SsiI CCGC 2 cut(s) 181, 504
SspMI CTAG 1 cut(s) 218
SstI GAGCTC 2 cut(s) 114, 255
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 2 cut(s) 270, 396
TaiI ACGT 2 cut(s) 93, 474
TaqI TCGA 3 cut(s) 114, 128, 450
TasI AATT 4 cut(s) 222, 301, 326, 456
Tru1I TTAA 2 cut(s) 51, 72
Tru9I TTAA 2 cut(s) 51, 72
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 2 cut(s) 251, 294
TspGWI ACGGA 1 cut(s) 113
Vha464I CTTAAG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 2 cut(s) 222, 301
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 505
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.