Rmu_ssc0000257.1_g000001

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000257.1
OG0020657
Physical Location & Seq
Reverse (-)
1 .. 763
763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000257.1_g000001.1.cds

Sequence Viewer

Length: 456 bp
atgaacaacgtgaggatctttagagccgagctcgaagctctccatttgaagataaaccttctcgaacctctgatcgctcagatagaggagttagacaagcggttgggccgtgaccagaaggaagctaaggaactactagaaatttcagaaaaaatgaacgggggggaagacctagttggcaagtgctccaaggttggctggtggaatatcttgaagaagaattcatacaccaacaaacttgtggaattggatggcaccctccaaagactattcgaaatactgaaactgcaagggttggtggttgggaggcagaccttgattgtagcaaagaggatagatgcccagatgaacgagcttgtacaaaatcagaatgagatgcagattttggtgaaagagattcatgattggatggccaacctaggtctatgggtagtcatgtttacagctatgttgttg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

152

Amino Acids

17.58

Weight (kDa)

6.16

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020657)

Species Orthologous Gene IDs
rosa_laevigata RLG00000017292 RLG00000017310
rosa_multiflora Rmu_ssc0000257.1_g000001
rosa_roxburghii Rroxscaffold_2G00139080
rosa_samantha Rh2AG169300 Rh2DG174700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AciI CCGC 1 cut(s) 100
AclWI GGATC 1 cut(s) 23
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 2 cut(s) 141, 220
AfaI GTAC 1 cut(s) 360
AgsI TTSAA 2 cut(s) 49, 214
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 5 cut(s) 31, 38, 125, 355, 446
AluI AGCT 5 cut(s) 31, 38, 125, 355, 446
Alw21I GWGCWC 2 cut(s) 33, 188
AlwI GGATC 1 cut(s) 23
AoxI GGCC 2 cut(s) 106, 411
ApoI RAATTY 2 cut(s) 141, 220
AspA2I CCTAGG 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 400
AsuII TTCGAA 1 cut(s) 273
AvrII CCTAGG 1 cut(s) 418
BalI TGGCCA 1 cut(s) 413
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 1 cut(s) 33
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 2 cut(s) 33, 188
BccI CCATC 2 cut(s) 245, 403
BceAI ACGGC 1 cut(s) 93
BfaI CTAG 3 cut(s) 137, 173, 419
BlnI CCTAGG 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 256
BmsI GCATC 2 cut(s) 328, 366
BpiI GAAGAC 1 cut(s) 174
BplI GAGNNNNNCTC 2 cut(s) 15, 47
Bpu10I CCTNAGC 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 189, 418
BseDI CCNNGG 2 cut(s) 189, 418
BseGI GGATG 2 cut(s) 256, 414
BseMII CTCAG 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 101
BshFI GGCC 2 cut(s) 108, 413
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 2 cut(s) 33, 188
BsnI GGCC 2 cut(s) 108, 413
Bsp119I TTCGAA 1 cut(s) 273
Bsp1286I GDGCHC 2 cut(s) 33, 188
Bsp1407I TGTACA 1 cut(s) 358
Bsp143I GATC 2 cut(s) 15, 72
BspACI CCGC 1 cut(s) 100
BspANI GGCC 2 cut(s) 108, 413
BspCNI CTCAG 1 cut(s) 91
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 23
BspT104I TTCGAA 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 358
BssECI CCNNGG 2 cut(s) 189, 418
BssMI GATC 2 cut(s) 15, 72
BssT1I CCWWGG 2 cut(s) 189, 418
BstAUI TGTACA 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 78, 126
BstF5I GGATG 2 cut(s) 256, 414
BstKTI GATC 2 cut(s) 18, 75
BstMBI GATC 2 cut(s) 15, 72
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BsuRI GGCC 2 cut(s) 108, 413
BtsCI GGATG 2 cut(s) 256, 414
CciI TCATGA 1 cut(s) 400
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 1 cut(s) 359
CviAII CATG 2 cut(s) 401, 436
CviJI RGCY 9 cut(s) 26, 31, 38, 108, 125, 198, 355, 413, 446
CviKI_1 RGCY 9 cut(s) 26, 31, 38, 108, 125, 198, 355, 413, 446
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 2 cut(s) 78, 126
DpnI GATC 2 cut(s) 17, 74
DpnII GATC 2 cut(s) 15, 72
EaeI YGGCCR 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 2 cut(s) 189, 418
Eco24I GRGCYC 1 cut(s) 33
Eco53kI GAGCTC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 220
EcoT14I CCWWGG 2 cut(s) 189, 418
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 189, 418
FaeI CATG 2 cut(s) 404, 439
FaiI YATR 5 cut(s) 226, 402, 427, 437, 449
FatI CATG 2 cut(s) 400, 435
FokI GGATG 2 cut(s) 263, 421
FriOI GRGCYC 1 cut(s) 33
FspBI CTAG 3 cut(s) 137, 173, 419
HaeIII GGCC 2 cut(s) 108, 413
Hin1II CATG 2 cut(s) 404, 439
HinfI GANTC 1 cut(s) 397
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 1 cut(s) 441
Hpy188I TCNGA 4 cut(s) 72, 81, 148, 369
Hpy188III TCNNGA 3 cut(s) 62, 211, 401
Hpy8I GTNNAC 1 cut(s) 441
HpyAV CCTTC 2 cut(s) 68, 112
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 289, 379
HpyF3I CTNAG 2 cut(s) 78, 126
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 2 cut(s) 404, 439
Kzo9I GATC 2 cut(s) 15, 72
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 3 cut(s) 128, 184, 356
LweI GCATC 2 cut(s) 328, 366
MaeI CTAG 3 cut(s) 137, 173, 419
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 1 cut(s) 110
MalI GATC 2 cut(s) 17, 74
MboI GATC 2 cut(s) 15, 72
MboII GAAGA 4 cut(s) 61, 179, 226, 229
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 2 cut(s) 33, 188
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 3 cut(s) 141, 220, 245
MluNI TGGCCA 1 cut(s) 413
MnlI CCTC 6 cut(s) 6, 78, 79, 269, 300, 324
Mox20I TGGCCA 1 cut(s) 413
MscI TGGCCA 1 cut(s) 413
Msp20I TGGCCA 1 cut(s) 413
NdeII GATC 2 cut(s) 15, 72
NlaIII CATG 2 cut(s) 404, 439
NlaIV GGNNCC 1 cut(s) 256
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 110
NspV TTCGAA 1 cut(s) 273
PagI TCATGA 1 cut(s) 400
PfeI GAWTC 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 15
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SacI GAGCTC 1 cut(s) 33
Sau3AI GATC 2 cut(s) 15, 72
Sau96I GGNCC 1 cut(s) 106
SduI GDGCHC 2 cut(s) 33, 188
SfaNI GCATC 2 cut(s) 328, 366
SfuI TTCGAA 1 cut(s) 273
Sse9I AATT 3 cut(s) 141, 220, 245
SsiI CCGC 1 cut(s) 100
SspMI CTAG 3 cut(s) 137, 173, 419
SstI GAGCTC 1 cut(s) 33
StyI CCWWGG 2 cut(s) 189, 418
TaiI ACGT 1 cut(s) 12
TaqI TCGA 3 cut(s) 33, 63, 273
TasI AATT 3 cut(s) 141, 220, 245
TatI WGTACW 1 cut(s) 358
TfiI GAWTC 1 cut(s) 397
TseFI GTSAC 1 cut(s) 110
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 5 cut(s) 17, 170, 213, 362, 389
XapI RAATTY 2 cut(s) 141, 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmaJI CCTAGG 1 cut(s) 418
XspI CTAG 3 cut(s) 137, 173, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.