RLG00000017310

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020657
Physical Location & Seq
Forward (+)
15124101 .. 15124882
782 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017310

Sequence Viewer

Length: 651 bp
ATGTCAGGAGTTGAAGGGCTTGTTGCAGGGGCTGCTATAAGTTTGCCATTTAATGCGTTGTATGATGTCCTTAAGGGAGTGATGAACAACGTCAGGATGTTTAGAGCCGAGCTCGAAGCTCTCCGTTTGAAGATAAACCTTCTCGGACCTCTGATCGCTCAGATAGAGGAGTTAGACAAGCGGTTGGGCCGTGACCAGAAGGAAGCTAAGGAACTACTAGAAATTTCAGAAAAAATGAACAAGGGGAAAGAGCTAGTTGGCAAGTGCTCCGAGGTTGGCTGGTGGAATATCCTGAAGAAGAATTCATACACCAACAAACTTGTGGAATTGGATGGCACCCTCCAAAGACTATTCGAAATACTGAAACTGCAAGGGTTGGTGGTTGGGAGGCAGACCTTGATTGTAGCTAAGAGGATAGACGCCCAGATGAATGCGCTTGTACAAAATCAGAATGAGATGCAGATGTTGGTGAAAGAGATTCATGATTGGATGGCCAACCTAGGTCCATGGGCGGTCATGTTTGCAGCTGTGTTGTTGAGCCATGTGGGGGCCAATGGCCGTCCATCTCCATATCCGTCTTGGCACTTCCAGCATTGTGGTCAGTTGTGTCTTTGTTTCAAGCCCATTGTGTTTTGGATGTTAGAGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

217

Amino Acids

24.47

Weight (kDa)

8.87

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 120 2.7e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020657)

Species Orthologous Gene IDs
rosa_laevigata RLG00000017292 RLG00000017310
rosa_multiflora Rmu_ssc0000257.1_g000001
rosa_roxburghii Rroxscaffold_2G00139080
rosa_samantha Rh2AG169300 Rh2DG174700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 335
AciI CCGC 2 cut(s) 181, 512
AcoI YGGCCR 2 cut(s) 492, 556
AcsI RAATTY 2 cut(s) 222, 301
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 1 cut(s) 441
AfeI AGCGCT 1 cut(s) 647
AfiI CCNNNNNNNGG 1 cut(s) 547
AflII CTTAAG 1 cut(s) 71
AgsI TTSAA 3 cut(s) 14, 130, 619
AluBI AGCT 6 cut(s) 112, 119, 206, 253, 407, 527
AluI AGCT 6 cut(s) 112, 119, 206, 253, 407, 527
Alw21I GWGCWC 2 cut(s) 114, 269
AlwNI CAGNNNCTG 1 cut(s) 32
Aor51HI AGCGCT 1 cut(s) 647
AoxI GGCC 4 cut(s) 187, 492, 549, 556
ApeKI GCWGC 2 cut(s) 32, 524
ApoI RAATTY 2 cut(s) 222, 301
AspA2I CCTAGG 1 cut(s) 499
AspLEI GCGC 2 cut(s) 436, 648
AspS9I GGNCC 4 cut(s) 146, 187, 503, 549
AsuHPI GGTGA 1 cut(s) 481
AsuII TTCGAA 1 cut(s) 354
AvaII GGWCC 2 cut(s) 146, 503
AvrII CCTAGG 1 cut(s) 499
BalI TGGCCA 1 cut(s) 494
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 114
BarI GAAGNNNNNNTAC 2 cut(s) 290, 322
Bbv12I GWGCWC 2 cut(s) 114, 269
BbvI GCAGC 2 cut(s) 19, 536
BccI CCATC 3 cut(s) 326, 484, 571
BceAI ACGGC 2 cut(s) 174, 543
BfaI CTAG 4 cut(s) 218, 254, 500, 649
BfoI RGCGCY 1 cut(s) 649
BfrI CTTAAG 1 cut(s) 71
BisI GCNGC 2 cut(s) 33, 525
BlnI CCTAGG 1 cut(s) 499
BlsI GCNGC 2 cut(s) 34, 526
Bme18I GGWCC 2 cut(s) 146, 503
BmgT120I GGNCC 4 cut(s) 146, 187, 503, 549
BmiI GGNNCC 2 cut(s) 337, 550
BmsI GCATC 1 cut(s) 447
BplI GAGNNNNNCTC 2 cut(s) 96, 128
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 3 cut(s) 270, 499, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 270, 499, 506
BseGI GGATG 4 cut(s) 102, 337, 495, 642
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 2 cut(s) 19, 536
BshFI GGCC 4 cut(s) 189, 494, 551, 558
BshNI GGYRCC 1 cut(s) 335
BsiHKAI GWGCWC 2 cut(s) 114, 269
BslI CCNNNNNNNGG 1 cut(s) 547
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 4 cut(s) 189, 494, 551, 558
Bsp119I TTCGAA 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 114, 269
Bsp1407I TGTACA 1 cut(s) 439
Bsp143I GATC 1 cut(s) 153
Bsp19I CCATGG 1 cut(s) 506
BspACI CCGC 2 cut(s) 181, 512
BspANI GGCC 4 cut(s) 189, 494, 551, 558
BspCNI CTCAG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 2 cut(s) 337, 550
BspT104I TTCGAA 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 335
BspTI CTTAAG 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 439
BssECI CCNNGG 3 cut(s) 270, 499, 506
BssMI GATC 1 cut(s) 153
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 499, 506
BstACI GRCGYC 1 cut(s) 420
BstAFI CTTAAG 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 439
BstBI TTCGAA 1 cut(s) 354
BstDEI CTNAG 3 cut(s) 159, 207, 408
BstDSI CCRYGG 1 cut(s) 506
BstF5I GGATG 4 cut(s) 102, 337, 495, 642
BstH2I RGCGCY 1 cut(s) 649
BstHHI GCGC 2 cut(s) 436, 648
BstKTI GATC 1 cut(s) 156
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 2 cut(s) 32, 589
BstV1I GCAGC 2 cut(s) 19, 536
BstXI CCANNNNNNTGG 1 cut(s) 596
BsuRI GGCC 4 cut(s) 189, 494, 551, 558
BtgI CCRYGG 1 cut(s) 506
BtsCI GGATG 4 cut(s) 102, 337, 495, 642
CaiI CAGNNNCTG 1 cut(s) 32
CciI TCATGA 1 cut(s) 481
CfoI GCGC 2 cut(s) 436, 648
Cfr13I GGNCC 4 cut(s) 146, 187, 503, 549
CseI GACGC 1 cut(s) 428
Csp6I GTAC 1 cut(s) 440
CviAII CATG 4 cut(s) 482, 507, 517, 542
CviQI GTAC 1 cut(s) 440
DdeI CTNAG 3 cut(s) 159, 207, 408
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 492, 556
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 2 cut(s) 499, 506
Eco24I GRGCYC 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 146, 503
Eco47III AGCGCT 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 499, 506
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 2 cut(s) 499, 506
FaeI CATG 4 cut(s) 485, 510, 520, 545
FaiI YATR 8 cut(s) 38, 63, 307, 483, 508, 518, 543, 571
FatI CATG 4 cut(s) 481, 506, 516, 541
Fnu4HI GCNGC 2 cut(s) 33, 525
FokI GGATG 3 cut(s) 109, 344, 502
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 2 cut(s) 33, 525
FspBI CTAG 4 cut(s) 218, 254, 500, 649
GlaI GCGC 2 cut(s) 435, 647
GluI GCNGC 2 cut(s) 33, 525
HaeII RGCGCY 1 cut(s) 649
HaeIII GGCC 4 cut(s) 189, 494, 551, 558
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 436, 648
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 4 cut(s) 485, 510, 520, 545
Hin6I GCGC 2 cut(s) 434, 646
HinP1I GCGC 2 cut(s) 434, 646
HinfI GANTC 1 cut(s) 478
HphI GGTGA 1 cut(s) 481
Hpy188I TCNGA 6 cut(s) 146, 153, 162, 229, 271, 450
Hpy188III TCNNGA 4 cut(s) 6, 94, 292, 482
HpyAV CCTTC 3 cut(s) 8, 149, 193
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 4 cut(s) 26, 370, 460, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 589
HpyF3I CTNAG 3 cut(s) 159, 207, 408
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 4 cut(s) 485, 510, 520, 545
HspAI GCGC 2 cut(s) 434, 646
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 7 cut(s) 12, 79, 209, 265, 305, 437, 602
Lsp1109I GCAGC 2 cut(s) 19, 536
LweI GCATC 1 cut(s) 447
MaeI CTAG 4 cut(s) 218, 254, 500, 649
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 191
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 3 cut(s) 142, 307, 310
MhlI GDGCHC 2 cut(s) 114, 269
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 3 cut(s) 222, 301, 326
MluNI TGGCCA 1 cut(s) 494
MnlI CCTC 6 cut(s) 159, 160, 265, 350, 381, 405
Mox20I TGGCCA 1 cut(s) 494
MscI TGGCCA 1 cut(s) 494
MseI TTAA 2 cut(s) 51, 72
Msp20I TGGCCA 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 527
MspCI CTTAAG 1 cut(s) 71
Mva1269I GAATGC 1 cut(s) 436
MwoI GCNNNNNNNGC 2 cut(s) 32, 589
NcoI CCATGG 1 cut(s) 506
NdeII GATC 1 cut(s) 153
NlaIII CATG 4 cut(s) 485, 510, 520, 545
NlaIV GGNNCC 2 cut(s) 337, 550
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 191
NspV TTCGAA 1 cut(s) 354
PagI TCATGA 1 cut(s) 481
PctI GAATGC 1 cut(s) 436
PfeI GAWTC 1 cut(s) 478
PkrI GCNGC 2 cut(s) 34, 526
Psp124BI GAGCTC 1 cut(s) 114
PspN4I GGNNCC 2 cut(s) 337, 550
PspPI GGNCC 4 cut(s) 146, 187, 503, 549
PstNI CAGNNNCTG 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 441
RsaNI GTAC 1 cut(s) 440
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 2 cut(s) 51, 72
SatI GCNGC 2 cut(s) 33, 525
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 4 cut(s) 146, 187, 503, 549
SduI GDGCHC 2 cut(s) 114, 269
SfaNI GCATC 1 cut(s) 447
SfuI TTCGAA 1 cut(s) 354
SinI GGWCC 2 cut(s) 146, 503
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 3 cut(s) 222, 301, 326
SsiI CCGC 2 cut(s) 181, 512
SspMI CTAG 4 cut(s) 218, 254, 500, 649
SstI GAGCTC 1 cut(s) 114
StyI CCWWGG 2 cut(s) 499, 506
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 114, 354
TasI AATT 3 cut(s) 222, 301, 326
TatI WGTACW 1 cut(s) 439
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 2 cut(s) 51, 72
Tru9I TTAA 2 cut(s) 51, 72
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 2 cut(s) 32, 524
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 5 cut(s) 98, 251, 294, 443, 470
TspGWI ACGGA 2 cut(s) 113, 564
Vha464I CTTAAG 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 146, 503
XapI RAATTY 2 cut(s) 222, 301
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 576
XmaJI CCTAGG 1 cut(s) 499
XspI CTAG 4 cut(s) 218, 254, 500, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.