Rh2AG169300

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0020657
Physical Location & Seq
Forward (+)
16032204 .. 16033659
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG169300.1

Sequence Viewer

Length: 624 bp
ATGTCAGGAGTTGAAGGGCTTATTGCAGGGGCTGCTATAAGTTTGCCATTTAATGCGTTGTATGATGTCCTTAAGGGAGTGATGAACAACATGAGGATGTTTAGAGCCCAGCTCGAAGCTCTCCGTTTGAAGATAAACCTTCTTGGACCTCTGATCGCTCAGATAGAGGAGTTAGACAAGCGGTTGGGCCGTGACCAGAAGGAAGCTACGGAACTACTAGAAATTTCAGAAAAAATGAACAAGGGGAAAGAGCTAGTTGGCAAGTGCTCCGAGGTTGGCTGGTGGAATATCCTGAAGAAGAATTCATACACCAACAAACTTGTGGAATTGGATGGCACCCTCCAAAGACTATTCGAAATACTGAAACTGCAAGGGTTGGTGGTTGGGAGGCAGACCTTGATTGTAGCTAAGAGGATAGACGCCCAGATGAATGCGCTTGTACAAAATCAGAATGAGATGCAGATGTTGGTGAAAGAGATTCATGATTGGATGGCCAACCTAGGTCCATGGGCGGTCATGTTTGTAGCTGTGTTGTTGGTGAGTATGGTCATGGTTATTGCTTCCAAAGACACAAAGTGGTTTTTGTTCAATGTTGGGAATAATACTTTAACATTCAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

207

Amino Acids

23.38

Weight (kDa)

8.97

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 120 9.8e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020657)

Species Orthologous Gene IDs
rosa_laevigata RLG00000017292 RLG00000017310
rosa_multiflora Rmu_ssc0000257.1_g000001
rosa_roxburghii Rroxscaffold_2G00139080
rosa_samantha Rh2AG169300 Rh2DG174700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 335
AciI CCGC 2 cut(s) 181, 512
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 2 cut(s) 222, 301
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 420
AdeI CACNNNGTG 1 cut(s) 576
AfaI GTAC 1 cut(s) 441
AflII CTTAAG 1 cut(s) 71
AgsI TTSAA 4 cut(s) 14, 130, 589, 616
AluBI AGCT 6 cut(s) 112, 119, 206, 253, 407, 527
AluI AGCT 6 cut(s) 112, 119, 206, 253, 407, 527
Alw21I GWGCWC 1 cut(s) 269
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 2 cut(s) 187, 492
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 2 cut(s) 222, 301
AspA2I CCTAGG 1 cut(s) 499
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 3 cut(s) 146, 187, 503
AsuHPI GGTGA 2 cut(s) 481, 550
AsuII TTCGAA 1 cut(s) 354
AvaII GGWCC 2 cut(s) 146, 503
AvrII CCTAGG 1 cut(s) 499
BalI TGGCCA 1 cut(s) 494
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 109
BarI GAAGNNNNNNTAC 2 cut(s) 290, 322
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 1 cut(s) 19
BccI CCATC 2 cut(s) 326, 484
BceAI ACGGC 1 cut(s) 174
BfaI CTAG 3 cut(s) 218, 254, 500
BfrI CTTAAG 1 cut(s) 71
BisI GCNGC 1 cut(s) 33
BlnI CCTAGG 1 cut(s) 499
BlsI GCNGC 1 cut(s) 34
Bme18I GGWCC 2 cut(s) 146, 503
BmgT120I GGNCC 3 cut(s) 146, 187, 503
BmiI GGNNCC 1 cut(s) 337
BmsI GCATC 1 cut(s) 447
BplI GAGNNNNNCTC 2 cut(s) 96, 128
Bpu14I TTCGAA 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 3 cut(s) 270, 499, 506
BseDI CCNNGG 3 cut(s) 270, 499, 506
BseGI GGATG 3 cut(s) 102, 337, 495
BseMII CTCAG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 19
BseYI CCCAGC 1 cut(s) 108
BshFI GGCC 2 cut(s) 189, 494
BshNI GGYRCC 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 2 cut(s) 189, 494
Bsp119I TTCGAA 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 109, 269
Bsp1407I TGTACA 1 cut(s) 439
Bsp143I GATC 1 cut(s) 153
Bsp19I CCATGG 1 cut(s) 506
BspACI CCGC 2 cut(s) 181, 512
BspANI GGCC 2 cut(s) 189, 494
BspCNI CTCAG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 337
BspT104I TTCGAA 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 335
BspTI CTTAAG 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 439
BssECI CCNNGG 3 cut(s) 270, 499, 506
BssMI GATC 1 cut(s) 153
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 499, 506
BstACI GRCGYC 1 cut(s) 420
BstAFI CTTAAG 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 439
BstBI TTCGAA 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 159, 408
BstDSI CCRYGG 1 cut(s) 506
BstF5I GGATG 3 cut(s) 102, 337, 495
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 1 cut(s) 156
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 19
BsuRI GGCC 2 cut(s) 189, 494
BtgI CCRYGG 1 cut(s) 506
BtsCI GGATG 3 cut(s) 102, 337, 495
CaiI CAGNNNCTG 1 cut(s) 32
CciI TCATGA 1 cut(s) 481
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 146, 187, 503
CseI GACGC 1 cut(s) 428
Csp6I GTAC 1 cut(s) 440
CviAII CATG 5 cut(s) 91, 482, 507, 517, 550
CviQI GTAC 1 cut(s) 440
DdeI CTNAG 2 cut(s) 159, 408
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
DraIII CACNNNGTG 1 cut(s) 576
EaeI YGGCCR 1 cut(s) 492
Eco130I CCWWGG 2 cut(s) 499, 506
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 146, 503
Eco57I CTGAAG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 499, 506
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 2 cut(s) 499, 506
FaeI CATG 5 cut(s) 94, 485, 510, 520, 553
FaiI YATR 9 cut(s) 38, 63, 92, 307, 483, 508, 518, 545, 551
FatI CATG 5 cut(s) 90, 481, 506, 516, 549
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 3 cut(s) 109, 344, 502
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 3 cut(s) 218, 254, 500
GlaI GCGC 1 cut(s) 435
GluI GCNGC 1 cut(s) 33
GsaI CCCAGC 1 cut(s) 112
HaeIII GGCC 2 cut(s) 189, 494
HgaI GACGC 1 cut(s) 428
HhaI GCGC 1 cut(s) 436
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 5 cut(s) 94, 485, 510, 520, 553
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HinfI GANTC 1 cut(s) 478
HphI GGTGA 2 cut(s) 481, 550
Hpy188I TCNGA 5 cut(s) 153, 162, 229, 271, 450
Hpy188III TCNNGA 3 cut(s) 6, 292, 482
HpyAV CCTTC 3 cut(s) 8, 149, 193
HpyCH4V TGCA 3 cut(s) 26, 370, 460
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 2 cut(s) 159, 408
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 5 cut(s) 94, 485, 510, 520, 553
HspAI GCGC 1 cut(s) 434
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 6 cut(s) 12, 122, 209, 265, 305, 437
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 447
MaeI CTAG 3 cut(s) 218, 254, 500
MaeIII GTNAC 1 cut(s) 191
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 3 cut(s) 142, 307, 310
MhlI GDGCHC 2 cut(s) 109, 269
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 4 cut(s) 222, 301, 326, 619
MluNI TGGCCA 1 cut(s) 494
MnlI CCTC 7 cut(s) 87, 159, 160, 265, 350, 381, 405
Mox20I TGGCCA 1 cut(s) 494
MscI TGGCCA 1 cut(s) 494
MseI TTAA 3 cut(s) 51, 72, 608
MslI CAYNNNNRTG 1 cut(s) 95
Msp20I TGGCCA 1 cut(s) 494
MspCI CTTAAG 1 cut(s) 71
Mva1269I GAATGC 1 cut(s) 436
MwoI GCNNNNNNNGC 1 cut(s) 32
NcoI CCATGG 1 cut(s) 506
NdeII GATC 1 cut(s) 153
NlaIII CATG 5 cut(s) 94, 485, 510, 520, 553
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 1 cut(s) 191
NspV TTCGAA 1 cut(s) 354
PagI TCATGA 1 cut(s) 481
PctI GAATGC 1 cut(s) 436
PfeI GAWTC 1 cut(s) 478
PkrI GCNGC 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 3 cut(s) 146, 187, 503
PstNI CAGNNNCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 441
RsaNI GTAC 1 cut(s) 440
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 3 cut(s) 51, 72, 608
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 3 cut(s) 146, 187, 503
SduI GDGCHC 2 cut(s) 109, 269
SfaNI GCATC 1 cut(s) 447
SfuI TTCGAA 1 cut(s) 354
SinI GGWCC 2 cut(s) 146, 503
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 4 cut(s) 222, 301, 326, 619
SsiI CCGC 2 cut(s) 181, 512
SspMI CTAG 3 cut(s) 218, 254, 500
StyI CCWWGG 2 cut(s) 499, 506
TaqI TCGA 2 cut(s) 114, 354
TasI AATT 4 cut(s) 222, 301, 326, 619
TatI WGTACW 1 cut(s) 439
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 3 cut(s) 51, 72, 608
Tru9I TTAA 3 cut(s) 51, 72, 608
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 5 cut(s) 98, 251, 294, 443, 470
TspGWI ACGGA 2 cut(s) 113, 224
Vha464I CTTAAG 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 146, 503
XapI RAATTY 2 cut(s) 222, 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XmaJI CCTAGG 1 cut(s) 499
XspI CTAG 3 cut(s) 218, 254, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.