RLG00000017656

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018776
Physical Location & Seq
Reverse (-)
19507075 .. 19508399
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017656

Sequence Viewer

Length: 441 bp
ATGGCTACTCTAAGATCAAGGCCATGGAGTGATTACAACACAAGCCATGGCCACGAAGCTTGGTCTTCTTCATGTCCCTGCAAACCCGGAATTCCCAATTACAGTTTGAACCTAAAGAATCAGAGATTTGGGACAGCTGGGTTAGCTCATCTGTGGCAGAAAATGGGAAAGAGATCAAGAGAAAGGTACTCGGTAGCTCAAGGATACGGTTCAGAAAGTCGTGAAACTTATGTTATTTCTGATCGAGACAAGAGATTCGTGAACGCTCTCCGGGAAGTTCAGCCGTATATTTTCCTCAACAGAGACCGGACATTTGTCGTGGTGGTCTCCGGTGAGATTGTTGCTAGTCCTTACTTCGATGCCATACTTCAGGATTTACTGGCCGAGATCAAGTATTTGATGTCTAGAGCAGACGCTCTGCGAGACTGGGAATTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.78

Weight (kDa)

9.17

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018776)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17830
rosa_laevigata RLG00000017655 RLG00000017656
rosa_multiflora Rmu_co8228899.1_g000001 Rmu_sc0007406.1_g000003
rosa_roxburghii Rroxscaffold_2G00135360
rosa_samantha Rh2AG200400 Rh2BG213100 Rh2CG205100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 49, 381
AcsI RAATTY 1 cut(s) 90
AcuI CTGAAG 1 cut(s) 353
AfaI GTAC 1 cut(s) 188
AgsI TTSAA 1 cut(s) 109
AluBI AGCT 4 cut(s) 59, 137, 146, 197
AluI AGCT 4 cut(s) 59, 137, 146, 197
Alw26I GTCTC 4 cut(s) 240, 297, 331, 417
AoxI GGCC 3 cut(s) 20, 49, 381
ApoI RAATTY 1 cut(s) 90
ArsI GACNNNNNNTTYG 4 cut(s) 239, 271, 296, 328
AsuC2I CCSGG 2 cut(s) 87, 272
AsuHPI GGTGA 1 cut(s) 344
BalI TGGCCA 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 57
BceAI ACGGC 1 cut(s) 268
BciVI GTATCC 1 cut(s) 197
BcnI CCSGG 2 cut(s) 87, 272
BcoDI GTCTC 4 cut(s) 240, 297, 331, 417
BfaI CTAG 2 cut(s) 345, 405
BfuI GTATCC 1 cut(s) 197
Bme1390I CCNGG 2 cut(s) 87, 272
BmrFI CCNGG 2 cut(s) 87, 272
BmrI ACTGGG 1 cut(s) 436
BmsI GCATC 1 cut(s) 349
BmuI ACTGGG 1 cut(s) 436
BoxI GACNNNNGTC 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 183
BpuMI CCSGG 2 cut(s) 87, 272
BsaI GGTCTC 2 cut(s) 297, 331
BsaJI CCNNGG 2 cut(s) 23, 46
BsaWI WCCGGW 2 cut(s) 306, 329
Bse1I ACTGG 2 cut(s) 384, 431
BseDI CCNNGG 2 cut(s) 23, 46
BseNI ACTGG 2 cut(s) 384, 431
BseYI CCCAGC 1 cut(s) 137
BshFI GGCC 3 cut(s) 22, 51, 383
BsiSI CCGG 4 cut(s) 87, 271, 307, 330
BslFI GGGAC 2 cut(s) 60, 145
BsmAI GTCTC 4 cut(s) 240, 297, 331, 417
BsmFI GGGAC 2 cut(s) 60, 145
BsnI GGCC 3 cut(s) 22, 51, 383
Bso31I GGTCTC 2 cut(s) 297, 331
Bsp143I GATC 4 cut(s) 14, 173, 241, 387
Bsp19I CCATGG 2 cut(s) 23, 46
BspANI GGCC 3 cut(s) 22, 51, 383
BspTNI GGTCTC 2 cut(s) 297, 331
BsrI ACTGG 2 cut(s) 384, 431
BssECI CCNNGG 2 cut(s) 23, 46
BssMI GATC 4 cut(s) 14, 173, 241, 387
BssT1I CCWWGG 2 cut(s) 23, 46
Bst4CI ACNGT 2 cut(s) 104, 209
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 2 cut(s) 23, 46
BstKTI GATC 4 cut(s) 17, 176, 244, 390
BstMAI GTCTC 4 cut(s) 240, 297, 331, 417
BstMBI GATC 4 cut(s) 14, 173, 241, 387
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstPAI GACNNNNGTC 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 85, 270
BstV2I GAAGAC 1 cut(s) 57
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 3 cut(s) 22, 51, 383
BtgI CCRYGG 2 cut(s) 23, 46
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 187
CspCI CAANNNNNGTGG 2 cut(s) 41, 76
CviAII CATG 3 cut(s) 24, 47, 72
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 1 cut(s) 11
DpnI GATC 4 cut(s) 16, 175, 243, 389
DpnII GATC 4 cut(s) 14, 173, 241, 387
EaeI YGGCCR 2 cut(s) 49, 381
Eco130I CCWWGG 2 cut(s) 23, 46
Eco31I GGTCTC 2 cut(s) 297, 331
Eco57I CTGAAG 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 90
EcoT14I CCWWGG 2 cut(s) 23, 46
ErhI CCWWGG 2 cut(s) 23, 46
FaeI CATG 3 cut(s) 27, 50, 75
FaiI YATR 6 cut(s) 25, 48, 73, 231, 288, 365
FaqI GGGAC 2 cut(s) 60, 145
FatI CATG 3 cut(s) 23, 46, 71
FspBI CTAG 2 cut(s) 345, 405
GsaI CCCAGC 1 cut(s) 141
HaeIII GGCC 3 cut(s) 22, 51, 383
HapII CCGG 4 cut(s) 87, 271, 307, 330
HgaI GACGC 1 cut(s) 422
Hin1II CATG 3 cut(s) 27, 50, 75
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 2 cut(s) 118, 255
HpaII CCGG 4 cut(s) 87, 271, 307, 330
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 3 cut(s) 123, 214, 241
Hpy188III TCNNGA 6 cut(s) 177, 221, 245, 259, 371, 405
Hpy8I GTNNAC 1 cut(s) 262
HpyCH4III ACNGT 2 cut(s) 104, 209
HpyCH4V TGCA 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 11
Hsp92II CATG 3 cut(s) 27, 50, 75
Kzo9I GATC 4 cut(s) 14, 173, 241, 387
LpnPI CCDG 9 cut(s) 91, 100, 123, 284, 320, 343, 356, 365, 412
LweI GCATC 1 cut(s) 349
MaeI CTAG 2 cut(s) 345, 405
MalI GATC 4 cut(s) 16, 175, 243, 389
MboI GATC 4 cut(s) 14, 173, 241, 387
MboII GAAGA 2 cut(s) 57, 60
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 3 cut(s) 90, 97, 431
MluNI TGGCCA 1 cut(s) 51
MnlI CCTC 1 cut(s) 305
Mox20I TGGCCA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 4 cut(s) 87, 271, 307, 330
MspR9I CCNGG 2 cut(s) 87, 272
MwoI GCNNNNNNNGC 1 cut(s) 143
NciI CCSGG 2 cut(s) 87, 272
NcoI CCATGG 2 cut(s) 23, 46
NdeII GATC 4 cut(s) 14, 173, 241, 387
NlaIII CATG 3 cut(s) 27, 50, 75
NmeAIII GCCGAG 1 cut(s) 409
PfeI GAWTC 2 cut(s) 118, 255
PfoI TCCNGGA 1 cut(s) 270
PshAI GACNNNNGTC 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
Sau3AI GATC 4 cut(s) 14, 173, 241, 387
ScrFI CCNGG 2 cut(s) 87, 272
SetI ASST 6 cut(s) 61, 114, 139, 148, 188, 199
SfaNI GCATC 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 3 cut(s) 90, 97, 431
SspMI CTAG 2 cut(s) 345, 405
StyD4I CCNGG 2 cut(s) 85, 270
StyI CCWWGG 2 cut(s) 23, 46
TaaI ACNGT 2 cut(s) 104, 209
TaqI TCGA 2 cut(s) 244, 357
TasI AATT 3 cut(s) 90, 97, 431
TfiI GAWTC 2 cut(s) 118, 255
TspDTI ATGAA 1 cut(s) 60
XapI RAATTY 1 cut(s) 90
XbaI TCTAGA 1 cut(s) 404
XspI CTAG 2 cut(s) 345, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.