Rh2AG200400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018776
Physical Location & Seq
Reverse (-)
20252736 .. 20253155
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG200400.1

Sequence Viewer

Length: 420 bp
ATGGCTACTCTAAGATCAAGGCCGTGGAGTGATTACAACACAAGCCATGGCCACGAAGCTTGGTCTTCTTCATGTCCCTGCAAACCCGGAATTCCCAATTACAGTTTGAACTTGAAGAATCCGAGAATTGGGACAGCCGGGTTGGCTCATCTGTGGCAGAAAATGGGAAAGAGATCAAGAGAAAGGTACTTGGTAGCTCAAGGGTACGGTTCAGAAAGTCATGAAGCTTATGTTATTTCCCACAAAGACAAGAGATTCGTGAACGCTCTTCGGGAAGTTCAGCCGTATATTTTCCTCAACAGAGGCCGGACATTTGTCGTGGTGGTCTCCGGCGAGATTGTTGCTAGTCCTTACTTCGATGCCATAATTCAGGTGAATATTTTTCTCCTAATTACACGTTTTGTTAAACTAATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.85

Weight (kDa)

9.89

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018776)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17830
rosa_laevigata RLG00000017655 RLG00000017656
rosa_multiflora Rmu_co8228899.1_g000001 Rmu_sc0007406.1_g000003
rosa_roxburghii Rroxscaffold_2G00135360
rosa_samantha Rh2AG200400 Rh2BG213100 Rh2CG205100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 1 cut(s) 90
AfaI GTAC 2 cut(s) 188, 206
AfiI CCNNNNNNNGG 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 395
AgsI TTSAA 2 cut(s) 109, 115
AluBI AGCT 3 cut(s) 59, 197, 227
AluI AGCT 3 cut(s) 59, 197, 227
Alw26I GTCTC 1 cut(s) 331
AoxI GGCC 3 cut(s) 20, 49, 304
ApoI RAATTY 1 cut(s) 90
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AseI ATTAAT 1 cut(s) 414
AsuC2I CCSGG 2 cut(s) 87, 139
AsuHPI GGTGA 1 cut(s) 385
BalI TGGCCA 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 57
BceAI ACGGC 2 cut(s) 7, 268
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BcnI CCSGG 2 cut(s) 87, 139
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 1 cut(s) 345
BglI GCCNNNNNGGC 1 cut(s) 143
Bme1390I CCNGG 2 cut(s) 87, 139
BmrFI CCNGG 2 cut(s) 87, 139
BmsI GCATC 1 cut(s) 349
BoxI GACNNNNGTC 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 183
BpuMI CCSGG 2 cut(s) 87, 139
BsaI GGTCTC 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 23, 46
Bsc4I CCNNNNNNNGG 1 cut(s) 128
BseDI CCNNGG 2 cut(s) 23, 46
BseLI CCNNNNNNNGG 1 cut(s) 128
BshFI GGCC 3 cut(s) 22, 51, 306
BsiSI CCGG 4 cut(s) 87, 138, 307, 330
BslFI GGGAC 2 cut(s) 60, 145
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 60, 145
BsnI GGCC 3 cut(s) 22, 51, 306
Bso31I GGTCTC 1 cut(s) 331
Bsp143I GATC 2 cut(s) 14, 173
Bsp19I CCATGG 1 cut(s) 46
BspANI GGCC 3 cut(s) 22, 51, 306
BspHI TCATGA 1 cut(s) 220
BspQI GCTCTTC 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 23, 46
BssMI GATC 2 cut(s) 14, 173
BssT1I CCWWGG 1 cut(s) 46
Bst4CI ACNGT 2 cut(s) 104, 209
Bst6I CTCTTC 1 cut(s) 273
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 2 cut(s) 23, 46
BstKTI GATC 2 cut(s) 17, 176
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 2 cut(s) 14, 173
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstPAI GACNNNNGTC 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 85, 137
BstV2I GAAGAC 1 cut(s) 57
BsuRI GGCC 3 cut(s) 22, 51, 306
BtgI CCRYGG 2 cut(s) 23, 46
CciI TCATGA 1 cut(s) 220
Csp6I GTAC 2 cut(s) 187, 205
CspCI CAANNNNNGTGG 2 cut(s) 41, 76
CviAII CATG 3 cut(s) 47, 72, 221
CviQI GTAC 2 cut(s) 187, 205
DdeI CTNAG 1 cut(s) 11
DpnI GATC 2 cut(s) 16, 175
DpnII GATC 2 cut(s) 14, 173
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 3 cut(s) 50, 75, 224
FaiI YATR 6 cut(s) 48, 73, 222, 231, 288, 365
FaqI GGGAC 2 cut(s) 60, 145
FatI CATG 3 cut(s) 46, 71, 220
FspBI CTAG 1 cut(s) 345
HaeIII GGCC 3 cut(s) 22, 51, 306
HapII CCGG 4 cut(s) 87, 138, 307, 330
Hin1II CATG 3 cut(s) 50, 75, 224
HindIII AAGCTT 2 cut(s) 57, 225
HinfI GANTC 2 cut(s) 118, 255
HpaII CCGG 4 cut(s) 87, 138, 307, 330
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 123, 214
Hpy188III TCNNGA 4 cut(s) 177, 221, 259, 272
Hpy8I GTNNAC 1 cut(s) 262
HpyCH4III ACNGT 2 cut(s) 104, 209
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 397
Hsp92II CATG 3 cut(s) 50, 75, 224
Kzo9I GATC 2 cut(s) 14, 173
LguI GCTCTTC 1 cut(s) 273
LpnPI CCDG 6 cut(s) 91, 100, 151, 320, 343, 356
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 345
MaeII ACGT 1 cut(s) 397
MalI GATC 2 cut(s) 16, 175
MboI GATC 2 cut(s) 14, 173
MboII GAAGA 4 cut(s) 57, 60, 127, 260
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 7 cut(s) 90, 97, 126, 366, 390, 411, 415
MluNI TGGCCA 1 cut(s) 51
MnlI CCTC 2 cut(s) 296, 305
Mox20I TGGCCA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 51
MseI TTAA 3 cut(s) 405, 414, 418
Msp20I TGGCCA 1 cut(s) 51
MspI CCGG 4 cut(s) 87, 138, 307, 330
MspR9I CCNGG 2 cut(s) 87, 139
MwoI GCNNNNNNNGC 1 cut(s) 143
NciI CCSGG 2 cut(s) 87, 139
NcoI CCATGG 1 cut(s) 46
NdeII GATC 2 cut(s) 14, 173
NlaIII CATG 3 cut(s) 50, 75, 224
PacI TTAATTAA 1 cut(s) 418
PagI TCATGA 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 118, 255
PshAI GACNNNNGTC 1 cut(s) 314
PshBI ATTAAT 1 cut(s) 414
RsaI GTAC 2 cut(s) 188, 206
RsaNI GTAC 2 cut(s) 187, 205
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 3 cut(s) 405, 414, 418
Sau3AI GATC 2 cut(s) 14, 173
ScrFI CCNGG 2 cut(s) 87, 139
SetI ASST 6 cut(s) 61, 188, 199, 229, 375, 400
SfaNI GCATC 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 7 cut(s) 90, 97, 126, 366, 390, 411, 415
SspI AATATT 1 cut(s) 379
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 2 cut(s) 85, 137
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 2 cut(s) 104, 209
TaiI ACGT 1 cut(s) 400
TaqI TCGA 1 cut(s) 357
TasI AATT 7 cut(s) 90, 97, 126, 366, 390, 411, 415
TfiI GAWTC 2 cut(s) 118, 255
Tru1I TTAA 3 cut(s) 405, 414, 418
Tru9I TTAA 3 cut(s) 405, 414, 418
TspDTI ATGAA 2 cut(s) 60, 237
VspI ATTAAT 1 cut(s) 414
XapI RAATTY 1 cut(s) 90
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.