RLG00000018326

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021553
Physical Location & Seq
Reverse (-)
28323825 .. 28327960
4136 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018326

Sequence Viewer

Length: 3243 bp
ATGCTTTCAGAGACCACTGAAGGAGATTCTTGCTCTTCTTCACTCACTCCTCAATGGAAGTACGATGTCTTTCTAAGTTTTAAAGGTGAAGATACGCGCTACAATTTCACAGACCATTTGTACAATGCTTTGCTTCAGAGGGGAATCAATGCCTTCATAGATAATGATCTTGCTAGAGGAGAAGAAATATCGGGAGAGCTTCTCAAAACAATTGAGGATTCAAGGATTTCTGTCATCATATTCTCTGAAAACTATGCATCGTCAAAGTGGTGTTTGGACGAACTTGTTAAGATCCTTGAATGTAAAGAATCAAAGAAACAAATGGTTTGGCCAATCTTTTACAAGGTGGATCCATCTGATGTGCGGAACCAAAGAGGGAGTTTTGGTGAGGCAATTGTTCAGCATGAACACAAGTTTAGTGATGACATTGAGAAAGTCAACAGATGGAGGGCAGCTCTTTCAAAAGCAGCAGATTTGTCAGGCAACCATTTCTTTGACGGTTATGAATCCAAATTTATCCATGAGGTGGTTGAGGAGATTTCGGTCAAGGTATTGCGCCGCACATATTTGAATGTGGCTAAGCATTCGGTTGGGATACAGCATTGTCAACAATGTGTGGAAGAGCTCTTAGGTGTTGGGGAAGATGATGTTCGAATGGTAGGGATATGGGGCACCGGTGGAATAGGTAAGACAACACTTGCTAAAGCAATTTATAATTCAAATGCTGATAAGTTTGAAGGTAGCTATTTTATGTATAATGTTAGAGAACATTCAATGATGGTTGGAGGCCTAGTCCAACTGCAGCAGGCCTTTCTATTTGAGGTCCTAGGAAAAAAAAGACTAGAGGTTGCTAATGTTGATAGAGGAATCAATGTGATAAAGGAAAGAATGAGAGATAAAAAAGTTCTCTTAATTCTAGATGATGTGAATCAACTGGATCAATTAAAAAACTTAGCCGGAGGGTGTGATTGGTTTGGTTTGGGCAGTAGAATTATCATAACGACTAGAGACAAGCGTTTGCTAGAAGTTCATCAGGTTCATTCAATATATGAGGTTGAGGGATTAGATTTTGATGAAGCCCTTGAGCTCTTCAGTTGGAATGCCTTCAAAAGAAATGCACCTTTCAATGATTATATGCAACTCGCAAAACTTGCAGTAATCAGTCCCCAGTGCCTTCCATTGGCTTTGACAATTTTAGGATCTCATCTATGTGGTAGAAGCATAGATCAATGGAAGGCTGCACTAGAGAGTTATAAAAGAGTTCCTAACAAAGAAATTCAAGGCATTCTCAAATTCAGTTATGATGGATTGGAATACTCTGAGAAGGAAGTGTTTCTTGATATTGCATGTTTCTTGAAAGGGAGGAATATGGACTATGTGAAGCAAATGCTAGAATCTTGTGACCTTGACCCTGTTGTTAGTATTGAAGTACTTAAAGAAAAGGCCCTCATAACAATTACTAAAATAGGCAATGGTGTTGAGATCGTTTGGATGCATGACTTGTTAGAAGAATTGGGAAAAGAGATAGTGCGCCAAGAATCACCCACTGAGCCAGGAAAGCGTAGCAGATTGTGGTTTCATGAAGATGTATACCATGTTCTAACAGAAAATACTGGAACAAAATCAGTTAAAGGCATCATGATTCAGTTGCCGCAACCAGAGGTGATATGTTTGAAATATATGCAAAATCTGAAGTCCATAAACTTTAAAGGTTGCAAGTTTCTCACAAGAATCCCCGACTTCTCTGGAATCCCAAGTTTAGAGAGATTGAACCTAAATCTTTGTACAGAATTACTTGAAGTTCATCCTTCTGTTGGATTCCTAGCCAAGCTTGTTAGCTTGAGTCTTTTGGACTGCTTCAACCTCGAAATCTTTCCAAGAAGACTCGATTTAAAATCTCTAGAAAGTATTGTTCTTCAAGGTTGCAGAAAACTTGAAACTTTCTCAGAAATTGTGGGGAAGATGGACAATCTGAGATATATGAATCTACAAGGCACTGCCATAAAAGGATTGCCTCTCTCTGTTGGAGATCTTAGTGGGCTTGAAATATTAATTCTCGATATTTGTGAAAGCCTTACAACCCTTCCATGCAGCATTTATGAGTTACATCATCTACGGGTTCTTCATCTCTCTAGGTGCTCAAATCTTTTTGCATTTCCTAGTAGAATGAACTATGAAGCATCATCCAGTGCGGTGCTGCTTCCTTTGCAGCTTTCAACTAACCAAAGCACTTTGGATGATACTTGTGGGTCAGCTGTTTCACATTGTGAACGAGATCACTACAATCACCAAAAAGGCTTGTCCACATTAGAATCACTTGATCTATCAAGAATCAATTTTGTTAGTCTTCCAGCATGGGTTAGCAAGTGTTCTAACTTGTTGCGGCTTAACTTGTGTTGGTGCAAAAGACTTCAAGAAATATCAGAACTTCCACCAAAGATAAGATGGTTGAGCGTCAGTGGTTGTAGCTCACTGGAAATATTGTCAATATTGTCAAACTTGGTGGAACGCAATGAGTTGCAAGGCCTTAGGTGGATCGATCTGTCTCATTGCCACAGGCTCCTTGACAATCTGTGCTATGATATAATAAAGATTGATAACGCTATTCGACGAAATCAGGTGCTATCTTCTGTGGATTTTGCTGTTTTACTTCCAGGAAGTAGAGTTCCACAACGGTTCAACCCTTTGACGAGTCGTAAGAGTGATGATTATGAGACTGAAAATGGTTATGATATTTGGGTTACAACATCTGCACCCTCGAAATTGGAAAACACAAGATTGACTCTATGTGCTGTTATAGGAATAACACAAAATGAGTCTGGTCCTGGTAGAATTTCAGTATCAAGTTGCATCAACAATCTAGAGATTGATGAATTTGAAGAATATATTTATCCCATAGACTGGTCAAGTACTGATCATGTGTGGCTGCGCTATATTTCATTATCAGGTATTGCTAATGATGACATGAGGGTTATCTCTAAAGTCAGATTTTCTTCTAAAGGGTCCGTGTCATTAAAAAGCTGTGGGATCCGTCTAGTATGCCCCCAGGATGATGATGATGATGATGGTGAGGACGACGATGACGAAGAGCAATGGCTTCCTAGTACTTCCTTGTCTGTGGAGCAAATGAATCCTCGAAAGCGGAAACATGTAGAGGAGGATCATAACCATGTCGTAGTCAGTAGTAGTACTGTTAATGTTGATGATGCTCCGCAGAAAAAGAAGTGGCTTTCTTTGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1081

Amino Acids

122.6

Weight (kDa)

5.48

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 184 3.6e-55 TIR domain
TIR_2 PF13676 23 - 118 5e-15 TIR domain
NB-ARC PF00931 204 - 372 9.7e-20 NB-ARC domain
WHD_ROQ1 PF23282 440 - 514 1.3e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 626 - 716 2.5e-09 Leucine-rich repeat region
LRR_13 PF23286 631 - 837 4.6e-17 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021553)

Species Orthologous Gene IDs
rosa_laevigata RLG00000018326
rosa_roxburghii Rroxscaffold_2G00126760 Rroxscaffold_2G00126780
rosa_samantha Rh2AG265800 Rh2DG273100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 714, 1254
AccB1I GGYRCC 1 cut(s) 671
AccB7I CCANNNNNTGG 2 cut(s) 526, 2901
AccI GTMKAC 1 cut(s) 1590
AccII CGCG 1 cut(s) 97
AciI CCGC 7 cut(s) 364, 559, 1652, 2192, 2383, 3142, 3212
AclWI GGATC 9 cut(s) 286, 344, 357, 945, 1207, 2543, 3022, 3035, 3168
AcoI YGGCCR 1 cut(s) 329
AcsI RAATTY 5 cut(s) 512, 1275, 1292, 2832, 2873
AcuI CTGAAG 4 cut(s) 39, 119, 1075, 1712
AdeI CACNNNGTG 1 cut(s) 2267
AfaI GTAC 7 cut(s) 62, 122, 1431, 1786, 2911, 3106, 3190
AfiI CCNNNNNNNGG 4 cut(s) 526, 1180, 1814, 2901
AflIII ACRYGT 1 cut(s) 3148
AgeI ACCGGT 1 cut(s) 674
AjnI CCWGG 4 cut(s) 1552, 2653, 2824, 3045
AloI GAACNNNNNNTCC 2 cut(s) 2649, 2681
Alw21I GWGCWC 3 cut(s) 627, 1089, 2141
Alw26I GTCTC 4 cut(s) 5, 1002, 2550, 2708
AlwI GGATC 9 cut(s) 286, 344, 357, 945, 1207, 2543, 3022, 3035, 3168
AoxI GGCC 5 cut(s) 329, 787, 807, 1443, 2524
ApeKI GCWGC 8 cut(s) 452, 467, 802, 1238, 2091, 2197, 2209, 2926
ApoI RAATTY 5 cut(s) 512, 1275, 1292, 2832, 2873
AseI ATTAAT 1 cut(s) 2051
AsiGI ACCGGT 1 cut(s) 674
Asp700I GAANNNNTTC 3 cut(s) 1103, 1332, 1872
AspA2I CCTAGG 1 cut(s) 826
AspLEI GCGC 4 cut(s) 99, 558, 1533, 2931
AspS9I GGNCC 4 cut(s) 823, 1444, 2822, 3003
AsuHPI GGTGA 6 cut(s) 98, 398, 1533, 1675, 2279, 3080
AsuII TTCGAA 1 cut(s) 652
AvaII GGWCC 3 cut(s) 823, 2822, 3003
AvrII CCTAGG 1 cut(s) 826
AxyI CCTNAGG 1 cut(s) 2528
BaeGI GKGCMC 1 cut(s) 674
BalI TGGCCA 1 cut(s) 331
BamHI GGATCC 2 cut(s) 349, 3027
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 2 cut(s) 627, 1089
BbsI GAAGAC 2 cut(s) 1888, 2339
Bbv12I GWGCWC 3 cut(s) 627, 1089, 2141
BbvI GCAGC 8 cut(s) 464, 479, 814, 1225, 2103, 2184, 2221, 2913
BccI CCATC 7 cut(s) 361, 438, 772, 1298, 1957, 2439, 3059
BciT130I CCWGG 4 cut(s) 1554, 2655, 2826, 3047
BciVI GTATCC 1 cut(s) 588
BclI TGATCA 1 cut(s) 2914
BcoDI GTCTC 4 cut(s) 5, 1002, 2550, 2708
BfmI CTRYAG 1 cut(s) 800
BfuI GTATCC 1 cut(s) 588
BglII AGATCT 1 cut(s) 2029
BlnI CCTAGG 1 cut(s) 826
BlpI GCTNAGC 1 cut(s) 579
BmcAI AGTACT 4 cut(s) 1431, 2911, 3106, 3190
Bme1390I CCNGG 4 cut(s) 1554, 2655, 2826, 3047
Bme18I GGWCC 3 cut(s) 823, 2822, 3003
BmgT120I GGNCC 4 cut(s) 823, 1444, 2822, 3003
BmiI GGNNCC 6 cut(s) 351, 368, 673, 2561, 3004, 3029
BmrFI CCNGG 4 cut(s) 1554, 2655, 2826, 3047
BmrI ACTGGG 1 cut(s) 1162
BmsI GCATC 6 cut(s) 266, 1482, 1644, 2189, 2859, 3196
BmuI ACTGGG 1 cut(s) 1162
BpiI GAAGAC 2 cut(s) 1888, 2339
BplI GAGNNNNNCTC 4 cut(s) 186, 218, 439, 471
Bpu1102I GCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 652
BpuEI CTTGAG 2 cut(s) 1103, 1861
Bsa29I ATCGAT 1 cut(s) 2538
BsaBI GATNNNNATC 2 cut(s) 165, 927
BsaI GGTCTC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 826, 3045
BsaWI WCCGGW 1 cut(s) 674
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 777, 807
Bsc4I CCNNNNNNNGG 4 cut(s) 526, 1180, 1814, 2901
Bse118I RCCGGY 1 cut(s) 674
Bse1I ACTGG 6 cut(s) 939, 1168, 1618, 2187, 2478, 2906
Bse21I CCTNAGG 1 cut(s) 2528
Bse3DI GCAATG 4 cut(s) 1477, 2518, 2548, 3098
Bse8I GATNNNNATC 2 cut(s) 165, 927
BseBI CCWGG 4 cut(s) 1554, 2655, 2826, 3047
BseCI ATCGAT 1 cut(s) 2538
BseDI CCNNGG 2 cut(s) 826, 3045
BseGI GGATG 5 cut(s) 1497, 1804, 2183, 2242, 3055
BseJI GATNNNNATC 2 cut(s) 165, 927
BseLI CCNNNNNNNGG 4 cut(s) 526, 1180, 1814, 2901
BseMI GCAATG 4 cut(s) 1477, 2518, 2548, 3098
BseMII CTCAG 4 cut(s) 1311, 1539, 1959, 1964
BseNI ACTGG 6 cut(s) 939, 1168, 1618, 2187, 2478, 2906
BseRI GAGGAG 4 cut(s) 39, 192, 548, 3170
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 8 cut(s) 464, 479, 814, 1225, 2103, 2184, 2221, 2913
BsgI GTGCAG 2 cut(s) 1224, 2736
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 5 cut(s) 331, 789, 809, 1445, 2526
BshNI GGYRCC 1 cut(s) 671
BshTI ACCGGT 1 cut(s) 674
BshVI ATCGAT 1 cut(s) 2538
BsiHKAI GWGCWC 3 cut(s) 627, 1089, 2141
BsiSI CCGG 2 cut(s) 675, 957
BslFI GGGAC 1 cut(s) 1149
BslI CCNNNNNNNGG 4 cut(s) 526, 1180, 1814, 2901
BsmAI GTCTC 4 cut(s) 5, 1002, 2550, 2708
BsmFI GGGAC 1 cut(s) 1149
BsmI GAATGC 3 cut(s) 583, 1105, 1284
BsnI GGCC 5 cut(s) 331, 789, 809, 1445, 2526
Bso31I GGTCTC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 652
Bsp1286I GDGCHC 4 cut(s) 627, 674, 1089, 2141
Bsp1407I TGTACA 2 cut(s) 120, 1784
Bsp1720I GCTNAGC 1 cut(s) 579
BspACI CCGC 7 cut(s) 364, 559, 1652, 2192, 2383, 3142, 3212
BspANI GGCC 5 cut(s) 331, 789, 809, 1445, 2526
BspCNI CTCAG 4 cut(s) 1312, 1540, 1958, 1965
BspDI ATCGAT 1 cut(s) 2538
BspFNI CGCG 1 cut(s) 97
BspHI TCATGA 2 cut(s) 1579, 1638
BspLI GGNNCC 6 cut(s) 351, 368, 673, 2561, 3004, 3029
BspMAI CTGCAG 1 cut(s) 804
BspPI GGATC 9 cut(s) 286, 344, 357, 945, 1207, 2543, 3022, 3035, 3168
BspQI GCTCTTC 4 cut(s) 40, 615, 1094, 3081
BspT104I TTCGAA 1 cut(s) 652
BspT107I GGYRCC 1 cut(s) 671
BspTNI GGTCTC 1 cut(s) 5
BsrDI GCAATG 4 cut(s) 1477, 2518, 2548, 3098
BsrFI RCCGGY 1 cut(s) 674
BsrGI TGTACA 2 cut(s) 120, 1784
BsrI ACTGG 6 cut(s) 939, 1168, 1618, 2187, 2478, 2906
BssAI RCCGGY 1 cut(s) 674
BssECI CCNNGG 2 cut(s) 826, 3045
BssNAI GTATAC 1 cut(s) 1591
BssT1I CCWWGG 1 cut(s) 826
Bst1107I GTATAC 1 cut(s) 1591
Bst2UI CCWGG 4 cut(s) 1554, 2655, 2826, 3047
Bst4CI ACNGT 3 cut(s) 500, 2676, 3193
Bst6I CTCTTC 4 cut(s) 40, 615, 1094, 3081
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstAUI TGTACA 2 cut(s) 120, 1784
BstBI TTCGAA 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 807
BstF5I GGATG 5 cut(s) 1497, 1804, 2183, 2242, 3055
BstFNI CGCG 1 cut(s) 97
BstHHI GCGC 4 cut(s) 99, 558, 1533, 2931
BstMAI GTCTC 4 cut(s) 5, 1002, 2550, 2708
BstMWI GCNNNNNNNGC 3 cut(s) 1151, 1558, 2206
BstNI CCWGG 4 cut(s) 1554, 2655, 2826, 3047
BstNSI RCATGY 2 cut(s) 1350, 3152
BstSCI CCNGG 4 cut(s) 1552, 2653, 2824, 3045
BstSFI CTRYAG 1 cut(s) 800
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 8 cut(s) 464, 479, 814, 1225, 2103, 2184, 2221, 2913
BstV2I GAAGAC 2 cut(s) 1888, 2339
BstX2I RGATCY 5 cut(s) 291, 349, 1199, 2029, 3027
BstYI RGATCY 5 cut(s) 291, 349, 1199, 2029, 3027
BstZ17I GTATAC 1 cut(s) 1591
Bsu15I ATCGAT 1 cut(s) 2538
Bsu36I CCTNAGG 1 cut(s) 2528
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 5 cut(s) 331, 789, 809, 1445, 2526
BsuTUI ATCGAT 1 cut(s) 2538
BtsCI GGATG 5 cut(s) 1497, 1804, 2183, 2242, 3055
BtsI GCAGTG 1 cut(s) 1995
BtsIMutI CAGTG 7 cut(s) 15, 1175, 1545, 1995, 2194, 2464, 2471
Cac8I GCNNGC 1 cut(s) 807
CciI TCATGA 2 cut(s) 1579, 1638
CfoI GCGC 4 cut(s) 99, 558, 1533, 2931
Cfr10I RCCGGY 1 cut(s) 674
Cfr13I GGNCC 4 cut(s) 823, 1444, 2822, 3003
ClaI ATCGAT 1 cut(s) 2538
CseI GACGC 1 cut(s) 2443
Csp6I GTAC 7 cut(s) 61, 121, 1430, 1785, 2910, 3105, 3189
CspAI ACCGGT 1 cut(s) 674
CspCI CAANNNNNGTGG 2 cut(s) 2484, 2519
CviQI GTAC 7 cut(s) 61, 121, 1430, 1785, 2910, 3105, 3189
DraI TTTAAA 3 cut(s) 82, 1708, 1893
DraIII CACNNNGTG 1 cut(s) 2267
EaeI YGGCCR 1 cut(s) 329
Eam1104I CTCTTC 4 cut(s) 40, 615, 1094, 3081
EarI CTCTTC 4 cut(s) 40, 615, 1094, 3081
Ecl136II GAGCTC 2 cut(s) 625, 1087
Eco130I CCWWGG 1 cut(s) 826
Eco147I AGGCCT 3 cut(s) 789, 809, 2526
Eco24I GRGCYC 2 cut(s) 627, 1089
Eco31I GGTCTC 1 cut(s) 5
Eco47I GGWCC 3 cut(s) 823, 2822, 3003
Eco53kI GAGCTC 2 cut(s) 625, 1087
Eco57I CTGAAG 4 cut(s) 39, 119, 1075, 1712
Eco81I CCTNAGG 1 cut(s) 2528
EcoICRI GAGCTC 2 cut(s) 625, 1087
EcoO109I RGGNCCY 2 cut(s) 823, 1444
EcoRII CCWGG 4 cut(s) 1552, 2653, 2824, 3045
EcoT14I CCWWGG 1 cut(s) 826
EcoT22I ATGCAT 2 cut(s) 259, 1497
EcoT38I GRGCYC 2 cut(s) 627, 1089
ErhI CCWWGG 1 cut(s) 826
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FaqI GGGAC 1 cut(s) 1149
FbaI TGATCA 1 cut(s) 2914
FblI GTMKAC 1 cut(s) 1590
FokI GGATG 5 cut(s) 1504, 1791, 2170, 2249, 3062
FriOI GRGCYC 2 cut(s) 627, 1089
GlaI GCGC 4 cut(s) 98, 557, 1532, 2930
HaeIII GGCC 5 cut(s) 331, 789, 809, 1445, 2526
HapII CCGG 2 cut(s) 675, 957
HgaI GACGC 1 cut(s) 2443
HhaI GCGC 4 cut(s) 99, 558, 1533, 2931
Hin6I GCGC 4 cut(s) 97, 556, 1531, 2929
HinP1I GCGC 4 cut(s) 97, 556, 1531, 2929
HincII GTYRAC 2 cut(s) 439, 608
HindII GTYRAC 2 cut(s) 439, 608
HindIII AAGCTT 1 cut(s) 1829
HpaII CCGG 2 cut(s) 675, 957
HphI GGTGA 6 cut(s) 98, 398, 1533, 1675, 2279, 3080
Hpy166II GTNNAC 5 cut(s) 439, 608, 1591, 2270, 2304
Hpy8I GTNNAC 5 cut(s) 439, 608, 1591, 2270, 2304
Hpy99I CGWCG 2 cut(s) 2613, 3080
HpyAV CCTTC 9 cut(s) 14, 163, 731, 1114, 1184, 1228, 1318, 1818, 2093
HpyCH4III ACNGT 3 cut(s) 500, 2676, 3193
HpyF10VI GCNNNNNNNGC 3 cut(s) 1151, 1558, 2206
HspAI GCGC 4 cut(s) 97, 556, 1531, 2929
Ksp22I TGATCA 1 cut(s) 2914
LguI GCTCTTC 4 cut(s) 40, 615, 1094, 3081
LmnI GCTCC 3 cut(s) 2565, 3121, 3214
Lsp1109I GCAGC 8 cut(s) 464, 479, 814, 1225, 2103, 2184, 2221, 2913
LweI GCATC 6 cut(s) 266, 1482, 1644, 2189, 2859, 3196
MaeIII GTNAC 3 cut(s) 1400, 2103, 2740
MfeI CAATTG 2 cut(s) 210, 393
MflI RGATCY 5 cut(s) 291, 349, 1199, 2029, 3027
MhlI GDGCHC 4 cut(s) 627, 674, 1089, 2141
MlsI TGGCCA 1 cut(s) 331
MluNI TGGCCA 1 cut(s) 331
MlyI GAGTC 5 cut(s) 1852, 1878, 2701, 2776, 2825
MmeI TCCRAC 5 cut(s) 763, 820, 1076, 1795, 2005
Mox20I TGGCCA 1 cut(s) 331
Mph1103I ATGCAT 2 cut(s) 259, 1497
MroXI GAANNNNTTC 3 cut(s) 1103, 1332, 1872
MscI TGGCCA 1 cut(s) 331
MslI CAYNNNNRTG 3 cut(s) 1209, 1584, 3168
Msp20I TGGCCA 1 cut(s) 331
MspA1I CMGCKG 1 cut(s) 2255
MspI CCGG 2 cut(s) 675, 957
MspR9I CCNGG 4 cut(s) 1554, 2655, 2826, 3047
MunI CAATTG 2 cut(s) 210, 393
Mva1269I GAATGC 3 cut(s) 583, 1105, 1284
MvaI CCWGG 4 cut(s) 1554, 2655, 2826, 3047
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 3 cut(s) 1151, 1558, 2206
NlaIV GGNNCC 6 cut(s) 351, 368, 673, 2561, 3004, 3029
NmuCI GTSAC 1 cut(s) 1400
NsiI ATGCAT 2 cut(s) 259, 1497
NspI RCATGY 2 cut(s) 1350, 3152
NspV TTCGAA 1 cut(s) 652
PagI TCATGA 2 cut(s) 1579, 1638
PceI AGGCCT 3 cut(s) 789, 809, 2526
PciI ACATGT 1 cut(s) 3148
PciSI GCTCTTC 4 cut(s) 40, 615, 1094, 3081
PcsI WCGNNNNNNNCGW 1 cut(s) 3081
PctI GAATGC 3 cut(s) 583, 1105, 1284
PdmI GAANNNNTTC 3 cut(s) 1103, 1332, 1872
PflMI CCANNNNNTGG 2 cut(s) 526, 2901
PfoI TCCNGGA 1 cut(s) 2653
PinAI ACCGGT 1 cut(s) 674
PleI GAGTC 5 cut(s) 1851, 1878, 2700, 2776, 2824
PpsI GAGTC 5 cut(s) 1851, 1878, 2700, 2776, 2824
PpuMI RGGWCCY 1 cut(s) 823
PscI ACATGT 1 cut(s) 3148
PshBI ATTAAT 1 cut(s) 2051
PsiI TTATAA 2 cut(s) 714, 1254
Psp124BI GAGCTC 2 cut(s) 627, 1089
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 4 cut(s) 1552, 2653, 2824, 3045
PspGI CCWGG 4 cut(s) 1552, 2653, 2824, 3045
PspN4I GGNNCC 6 cut(s) 351, 368, 673, 2561, 3004, 3029
PspPI GGNCC 4 cut(s) 823, 1444, 2822, 3003
PspPPI RGGWCCY 1 cut(s) 823
PsrI GAACNNNNNNTAC 2 cut(s) 1581, 1613
PstI CTGCAG 1 cut(s) 804
PsuI RGATCY 5 cut(s) 291, 349, 1199, 2029, 3027
PvuII CAGCTG 1 cut(s) 2255
RsaI GTAC 7 cut(s) 62, 122, 1431, 1786, 2911, 3106, 3190
RsaNI GTAC 7 cut(s) 61, 121, 1430, 1785, 2910, 3105, 3189
RseI CAYNNNNRTG 3 cut(s) 1209, 1584, 3168
SacI GAGCTC 2 cut(s) 627, 1089
SapI GCTCTTC 4 cut(s) 40, 615, 1094, 3081
Sau96I GGNCC 4 cut(s) 823, 1444, 2822, 3003
ScaI AGTACT 4 cut(s) 1431, 2911, 3106, 3190
SchI GAGTC 5 cut(s) 1852, 1878, 2701, 2776, 2825
ScrFI CCNGG 4 cut(s) 1554, 2655, 2826, 3047
SduI GDGCHC 4 cut(s) 627, 674, 1089, 2141
SfaNI GCATC 6 cut(s) 266, 1482, 1644, 2189, 2859, 3196
SfcI CTRYAG 1 cut(s) 800
SfuI TTCGAA 1 cut(s) 652
SgrAI CRCCGGYG 1 cut(s) 674
SinI GGWCC 3 cut(s) 823, 2822, 3003
SmiMI CAYNNNNRTG 3 cut(s) 1209, 1584, 3168
SmlI CTYRAG 2 cut(s) 1082, 1840
SmoI CTYRAG 2 cut(s) 1082, 1840
SseBI AGGCCT 3 cut(s) 789, 809, 2526
SsiI CCGC 7 cut(s) 364, 559, 1652, 2192, 2383, 3142, 3212
SspI AATATT 3 cut(s) 2049, 2481, 2490
SstI GAGCTC 2 cut(s) 627, 1089
StuI AGGCCT 3 cut(s) 789, 809, 2526
StyD4I CCNGG 4 cut(s) 1552, 2653, 2824, 3045
StyI CCWWGG 1 cut(s) 826
TaaI ACNGT 3 cut(s) 500, 2676, 3193
TaqI TCGA 8 cut(s) 652, 1866, 1887, 2058, 2538, 2608, 2759, 3136
TaqII GACCGA 1 cut(s) 532
TatI WGTACW 6 cut(s) 120, 1429, 1784, 2909, 3104, 3188
TauI GCSGC 3 cut(s) 561, 1654, 2386
TscAI CASTG 7 cut(s) 22, 1175, 1552, 2002, 2194, 2464, 2478
TseFI GTSAC 1 cut(s) 1400
TseI GCWGC 8 cut(s) 452, 467, 802, 1238, 2091, 2197, 2209, 2926
Tsp45I GTSAC 1 cut(s) 1400
TspGWI ACGGA 2 cut(s) 2995, 3020
TspRI CASTG 7 cut(s) 22, 1175, 1552, 2002, 2194, 2464, 2478
Van91I CCANNNNNTGG 2 cut(s) 526, 2901
VpaK11BI GGWCC 3 cut(s) 823, 2822, 3003
VspI ATTAAT 1 cut(s) 2051
XapI RAATTY 5 cut(s) 512, 1275, 1292, 2832, 2873
XbaI TCTAGA 3 cut(s) 916, 1900, 2860
XceI RCATGY 2 cut(s) 1350, 3152
XcmI CCANNNNNNNNNTGG 1 cut(s) 2442
XmaJI CCTAGG 1 cut(s) 826
XmiI GTMKAC 1 cut(s) 1590
XmnI GAANNNNTTC 3 cut(s) 1103, 1332, 1872
ZrmI AGTACT 4 cut(s) 1431, 2911, 3106, 3190
Zsp2I ATGCAT 2 cut(s) 259, 1497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.