Rroxscaffold_2G00126780

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0021553
Physical Location & Seq
Forward (+)
62168458 .. 62172723
4266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126780.1

Sequence Viewer

Length: 3039 bp
ATGCTTTCAGATACCACTGTAGGAGATTCCTCCTCTTCTTCACTCACTCCTCAATGGAAGTACGTTGTCTGTCTAAGTTTTAGAGGTGAAGATACGCGCTATAATTTCACAGACCATTTGTACAATGCATTGCTTCAGAGGGGAATCAACACCTTTATAGATAATGATCTTGCTAGAGGAGAAGACATATCAGGAGAGCTTCTCAAAACAATTGAGGATTCAAGGATTTCAGTCATTGTATTCTCTGAAAACTATGCATCGTCAAAGTGGTGTTTGGACGAACTTGTGGATCCATCTGATGTGCGGAACCAAAGAGGTAGTTTTGGTGAGGCAATTATGCAGCATGAACACAAGTTTAAGGATGACATTGAAAAAGTCAACAGATGGAGGGAGCTCTCTCAAAAGCAGCAGATTTGTCAGGCAGCCATTTCTTTGACGGGTATGTTTGTACAAGAATACAGTTATGAATCCAGATTTATCCATGAGGTGGTTGAGGAGATTTCGGTCAAGGTACTACACCGCACCTATTTGAATGTGGCTAAGCATCCAGTTGGGATACAACGTCGTCATCAATGTGTGGAAGACCTCTTAGGTGTTGGGGGAAATGATGTTCGAATGGTAGGGATATGGGGCATCGGTGGAATTGGTAAGACAACACTTGCTAAAGCTATTTATAATTCAAATGCGGATAAGTTTGAAGGTAGCTATTTTATGTATAATGTTAGAGAACATTCAACCATGGTTGGAGGCCTGGTCCAACTACAGCAGTCTTTTCTATCTGAGATTATGGGAAAAAAGACGCTAGCAGTGGCTAATGTTGACAGAGGAATCAATGTGATAAAGGAAAGAGTGAGAAATAAAAAAGTTCTCTTAATTCTTGATGATGTCAATCAATTGGACCAGTTAAACAACTTAGCCGGAGGGTGTGATTGGTTTGGTTCTGGCAGTAGAATTATCATAACAACTAGAGATAAGCATTTGCTAGATGTTCATCAAGTTCATTCAATCTATGAGGTTGAGGGGTTAGATTTTGATGAAGCTCTCGAGCTCTTCAGTTGGAATGCCTTCAAAAGAAATATACCTTTAGATGATTATATGGAACTTTCAGAACATGCAGTAAACAATGCCCAACGCCTTCCATTGGCTTTGACAATTTTAGGTTCTCATCTTTGTGGTAGAAGCATAGATCAATGGAAGGCTGCACTAGAGAGTTACAAAAGAGTTCCTAACAAAGACATTCAAGGCATTCTCAAAATAAGTTATGATGGATTGGATTACTCTGAGAAGGAAGTGTTTCTTGATATTGCATGTTTCTTGAAAGGGAGGAATATGGACTATGTGAAGCAAATACTAGAATCGTGTGACCTCGACCCTGTCATTGGTATTGAAGTACTCAAAGAAAAGGCCCTCATAACTATTACTAAATGGAGCCATGGTATTGAGATCATTTGGATGCATGACTTGTTAGAAGAATTGGGAAAAGAGATAAATATGCAATATCTGAAGTCTATTAACTTGAGATTTTGTAAGTACCTCACAAGAGTCCCCGACTTTTCTGGAATCCCAAGTTTAGAGAGGTTGAACCTAAATCTTTGTAGACAATTAGTTGAAGTTCATCCTTCTGTTGGAGTCCTAGCTAAGCTTGTTAGCTTGAGTCTTGTGAAGTGCTCCAACCTTGAGAAGTTCCCAAGAAGACTCAAGTTAAGATCTCTAGAAAGTATTGTTCTTCAAGGTTGCAGAAAGCTTGAGAGTTTCTCAGAAATTGTGGGGAAGATGGACAATTTGAGATATATGAATCTACAGGGAACTTCAATAAAAGAATTGCCTCCCTCGATTGGATATCTCAGTGGGCTTGAAATATTAATTCTCGATACTTGTGAAAGCCTTACAAATCTTCCATGCAGCATTTATGAATTGCATCATCTAAGGGTTCTTCGTCTCTCTAGGTGCTCAAACCTTGTTGAATTTCCAAGTAGAATGAACTCTGGAGCTTCGTCAAGTGCAGGGCCACTTCCATTGCTTCTTTCAAATAATCAAAGCCATGATATTAGTGACTCAGCTGTGTCACCTTGTGAGCAAGTTCATTTCAATCCCAAAAGAGGCTTGTCCACATTAGAATCACTTGATCTATCAAGAAGCAATTTTGTTAGTCTTCCTGTATGGATTAGCAAGTGTTCTAACTTGTTGCGGCTTAACTTGTGTCGTTGCAAGAAACTTCAAGAAATTTCAGAACTTCCACCAAAGATTAGATGGCTAAGCGTCGGTGGTTGTGACTCACTGGAAATATTGCCAATATTGTCCAACTTGGTGGAACACAATGTGTTGCAAGGCCTTCAGTGGATCGACCTGTCTCATTGCCACAGGCTCCTTGACAATCTTTGCTATGACACAACAAAGATCGATAACGCTATTCGACAAAATCAGGTGCTATCTTGTCTGGATTTTGGTGTTATACTTCCAGGAAGTGAAGTTCCCCACCGGTTCAACCCTTTGAGGAGTTGCAGGAGTTATGAGATTAAAAATTATTGTGACATTTGCATTATAATACCTCCACCTTTGATTTGGAAAAACGCAAGATTGACTCTATGTGCTGTTATAGGAATAACACAAAATGAGTCTGGTCCCGGTAGAATTTCAGCCTCAAGTTACATCAACCAGCTAGTGATCGATGAATTTGAAGAATATATCTATCCCTCAGACGAGTCAAGTACTGATCATGTGTGGTTGCGCTATATTTCGTTATCAGGTATTGCTTGTGATGACATGATCCTTAACTGTCAAGCTATATTTTCTTCTGAAGGGTCCGTGGCGTTAAAAAGCTGTGGAATTCGTCTAGTCTATCCCCAGGATGATGATGATGATGATGATGATGGGAATGACGAAGAGCAATGGCTTCCTAGTACTTCTTTGTCTTTGGAACCAATGAATCCTCGAAAGCGGAAACATATAGAGGAGGATCATAACCTCGTCACAGTCAGCAGTCGTACTGCTAATGTTGATGATGCTCCACAGAAAGAGAAGTGGCTTTCGTTGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1012

Amino Acids

114.52

Weight (kDa)

5.57

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 96 3.3e-27 TIR domain
TIR_2 PF13676 23 - 95 2.3e-11 TIR domain
TIR PF01582 96 - 173 5.4e-07 TIR domain
NB-ARC PF00931 201 - 359 6.9e-19 NB-ARC domain
ATPase_2 PF01637 207 - 304 3.5e-06 ATPase domain predominantly from Archaea
WHD_ROQ1 PF23282 427 - 496 2.8e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 550 - 653 7.6e-12 Leucine-rich repeat region
LRR_13 PF23286 568 - 768 2.9e-17 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021553)

Species Orthologous Gene IDs
rosa_laevigata RLG00000018326
rosa_roxburghii Rroxscaffold_2G00126760 Rroxscaffold_2G00126780
rosa_samantha Rh2AG265800 Rh2DG273100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 675, 2543
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 1 cut(s) 1597
AccII CGCG 1 cut(s) 97
AciI CCGC 5 cut(s) 304, 520, 686, 2188, 2938
AclWI GGATC 5 cut(s) 284, 297, 2348, 2761, 2964
AcsI RAATTY 5 cut(s) 1964, 2223, 2631, 2672, 2826
AcuI CTGAAG 5 cut(s) 119, 1036, 1523, 2318, 2817
AdeI CACNNNGTG 2 cut(s) 2072, 2320
AfaI GTAC 9 cut(s) 62, 122, 450, 513, 1392, 1532, 2710, 2902, 2986
AfiI CCNNNNNNNGG 6 cut(s) 487, 1141, 1379, 1625, 1835, 2099
AgeI ACCGGT 1 cut(s) 2478
AjnI CCWGG 3 cut(s) 750, 2458, 2844
AloI GAACNNNNNNTCC 4 cut(s) 594, 626, 2454, 2486
Alw21I GWGCWC 4 cut(s) 396, 1050, 1670, 1952
Alw26I GTCTC 2 cut(s) 1943, 2355
AlwI GGATC 5 cut(s) 284, 297, 2348, 2761, 2964
Ama87I CYCGRG 1 cut(s) 1043
AoxI GGCC 4 cut(s) 748, 1404, 2006, 2329
ApeKI GCWGC 5 cut(s) 340, 406, 422, 1199, 1902
ApoI RAATTY 5 cut(s) 1964, 2223, 2631, 2672, 2826
AseI ATTAAT 1 cut(s) 1862
AsiGI ACCGGT 1 cut(s) 2478
Asp700I GAANNNNTTC 2 cut(s) 1064, 1293
AspLEI GCGC 2 cut(s) 99, 2730
AspS9I GGNCC 6 cut(s) 754, 898, 1405, 2006, 2621, 2802
AsuC2I CCSGG 1 cut(s) 2625
AsuHPI GGTGA 3 cut(s) 98, 338, 2058
AsuII TTCGAA 1 cut(s) 613
AsuNHI GCTAGC 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 1043
AvaII GGWCC 4 cut(s) 754, 898, 2621, 2802
BaeI ACNNNNGTAYC 1 cut(s) 36
BamHI GGATCC 1 cut(s) 289
BanII GRGCYC 2 cut(s) 396, 1050
BbsI GAAGAC 4 cut(s) 189, 588, 1699, 2144
Bbv12I GWGCWC 4 cut(s) 396, 1050, 1670, 1952
BbvI GCAGC 5 cut(s) 352, 418, 434, 1186, 1914
BccI CCATC 6 cut(s) 301, 378, 1259, 1768, 2244, 2864
BciT130I CCWGG 3 cut(s) 752, 2460, 2846
BciVI GTATCC 1 cut(s) 549
BclI TGATCA 1 cut(s) 2713
BcnI CCSGG 1 cut(s) 2625
BcoDI GTCTC 2 cut(s) 1943, 2355
BfmI CTRYAG 3 cut(s) 18, 761, 1799
BfuI GTATCC 1 cut(s) 549
BglII AGATCT 1 cut(s) 1706
BisI GCNGC 6 cut(s) 341, 407, 423, 1200, 1903, 2189
BlpI GCTNAGC 3 cut(s) 540, 1638, 2255
BlsI GCNGC 6 cut(s) 342, 408, 424, 1201, 1904, 2190
BmcAI AGTACT 3 cut(s) 1392, 2710, 2902
Bme1390I CCNGG 4 cut(s) 752, 2460, 2625, 2846
Bme18I GGWCC 4 cut(s) 754, 898, 2621, 2802
BmeT110I CYCGRG 1 cut(s) 1043
BmgT120I GGNCC 6 cut(s) 754, 898, 1405, 2006, 2621, 2802
BmiI GGNNCC 7 cut(s) 291, 308, 1430, 2366, 2623, 2803, 2919
BmrFI CCNGG 4 cut(s) 752, 2460, 2625, 2846
BmsI GCATC 6 cut(s) 266, 553, 642, 1443, 1927, 2992
BmtI GCTAGC 1 cut(s) 806
BpiI GAAGAC 4 cut(s) 189, 588, 1699, 2144
BplI GAGNNNNNCTC 4 cut(s) 186, 218, 1739, 1771
BpmI CTGGAG 1 cut(s) 2007
Bpu1102I GCTNAGC 3 cut(s) 540, 1638, 2255
Bpu14I TTCGAA 1 cut(s) 613
BpuEI CTTGAG 6 cut(s) 1537, 1672, 1682, 1697, 1766, 2626
BpuMI CCSGG 1 cut(s) 2625
Bsa29I ATCGAT 2 cut(s) 2400, 2667
BsaBI GATNNNNATC 3 cut(s) 165, 888, 990
BsaJI CCNNGG 4 cut(s) 738, 1432, 2805, 2844
BsaWI WCCGGW 1 cut(s) 2478
BsaXI ACNNNNNCTCC 4 cut(s) 738, 768, 1420, 1450
Bsc4I CCNNNNNNNGG 6 cut(s) 487, 1141, 1379, 1625, 1835, 2099
Bse118I RCCGGY 1 cut(s) 2478
Bse1I ACTGG 3 cut(s) 548, 901, 2283
Bse3DI GCAATG 4 cut(s) 128, 2015, 2353, 2894
Bse8I GATNNNNATC 3 cut(s) 165, 888, 990
BseBI CCWGG 3 cut(s) 752, 2460, 2846
BseCI ATCGAT 2 cut(s) 2400, 2667
BseDI CCNNGG 4 cut(s) 738, 1432, 2805, 2844
BseGI GGATG 5 cut(s) 367, 544, 1458, 1615, 2854
BseJI GATNNNNATC 3 cut(s) 165, 888, 990
BseLI CCNNNNNNNGG 6 cut(s) 487, 1141, 1379, 1625, 1835, 2099
BseMI GCAATG 4 cut(s) 128, 2015, 2353, 2894
BseMII CTCAG 6 cut(s) 771, 1272, 1770, 1858, 2070, 2709
BseNI ACTGG 3 cut(s) 548, 901, 2283
BseRI GAGGAG 6 cut(s) 22, 39, 192, 509, 2509, 2966
BseXI GCAGC 5 cut(s) 352, 418, 434, 1186, 1914
BsgI GTGCAG 2 cut(s) 1185, 2022
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 4 cut(s) 750, 1406, 2008, 2331
BshTI ACCGGT 1 cut(s) 2478
BshVI ATCGAT 2 cut(s) 2400, 2667
BsiHKAI GWGCWC 4 cut(s) 396, 1050, 1670, 1952
BsiHKCI CYCGRG 1 cut(s) 1043
BsiSI CCGG 3 cut(s) 918, 2479, 2625
BslFI GGGAC 2 cut(s) 1529, 2607
BslI CCNNNNNNNGG 6 cut(s) 487, 1141, 1379, 1625, 1835, 2099
BsmAI GTCTC 2 cut(s) 1943, 2355
BsmBI CGTCTC 1 cut(s) 1943
BsmFI GGGAC 2 cut(s) 1529, 2607
BsmI GAATGC 2 cut(s) 1066, 1245
BsnI GGCC 4 cut(s) 750, 1406, 2008, 2331
BsoBI CYCGRG 1 cut(s) 1043
Bsp119I TTCGAA 1 cut(s) 613
Bsp1286I GDGCHC 4 cut(s) 396, 1050, 1670, 1952
Bsp1407I TGTACA 2 cut(s) 120, 448
Bsp1720I GCTNAGC 3 cut(s) 540, 1638, 2255
Bsp19I CCATGG 2 cut(s) 738, 1432
BspACI CCGC 5 cut(s) 304, 520, 686, 2188, 2938
BspANI GGCC 4 cut(s) 750, 1406, 2008, 2331
BspCNI CTCAG 6 cut(s) 772, 1273, 1769, 1857, 2069, 2708
BspDI ATCGAT 2 cut(s) 2400, 2667
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 7 cut(s) 291, 308, 1430, 2366, 2623, 2803, 2919
BspOI GCTAGC 1 cut(s) 806
BspPI GGATC 5 cut(s) 284, 297, 2348, 2761, 2964
BspQI GCTCTTC 2 cut(s) 1055, 2877
BspT104I TTCGAA 1 cut(s) 613
BsrDI GCAATG 4 cut(s) 128, 2015, 2353, 2894
BsrFI RCCGGY 1 cut(s) 2478
BsrGI TGTACA 2 cut(s) 120, 448
BsrI ACTGG 3 cut(s) 548, 901, 2283
BssAI RCCGGY 1 cut(s) 2478
BssECI CCNNGG 4 cut(s) 738, 1432, 2805, 2844
BssT1I CCWWGG 2 cut(s) 738, 1432
Bst2UI CCWGG 3 cut(s) 752, 2460, 2846
Bst4CI ACNGT 4 cut(s) 19, 461, 2777, 2974
Bst6I CTCTTC 3 cut(s) 40, 1055, 2877
BstAUI TGTACA 2 cut(s) 120, 448
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 1 cut(s) 804
BstDSI CCRYGG 3 cut(s) 738, 1432, 2805
BstF5I GGATG 5 cut(s) 367, 544, 1458, 1615, 2854
BstFNI CGCG 1 cut(s) 97
BstHHI GCGC 2 cut(s) 99, 2730
BstMAI GTCTC 2 cut(s) 1943, 2355
BstNI CCWGG 3 cut(s) 752, 2460, 2846
BstNSI RCATGY 2 cut(s) 1115, 1311
BstSCI CCNGG 4 cut(s) 750, 2458, 2623, 2844
BstSFI CTRYAG 3 cut(s) 18, 761, 1799
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 5 cut(s) 352, 418, 434, 1186, 1914
BstV2I GAAGAC 4 cut(s) 189, 588, 1699, 2144
BstX2I RGATCY 2 cut(s) 289, 1706
BstXI CCANNNNNNTGG 1 cut(s) 2308
BstYI RGATCY 2 cut(s) 289, 1706
Bsu15I ATCGAT 2 cut(s) 2400, 2667
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 4 cut(s) 750, 1406, 2008, 2331
BsuTUI ATCGAT 2 cut(s) 2400, 2667
BtgI CCRYGG 3 cut(s) 738, 1432, 2805
BtsCI GGATG 5 cut(s) 367, 544, 1458, 1615, 2854
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 5 cut(s) 15, 813, 1852, 2276, 2342
Cac8I GCNNGC 1 cut(s) 804
CfoI GCGC 2 cut(s) 99, 2730
Cfr10I RCCGGY 1 cut(s) 2478
Cfr13I GGNCC 6 cut(s) 754, 898, 1405, 2006, 2621, 2802
ClaI ATCGAT 2 cut(s) 2400, 2667
CseI GACGC 2 cut(s) 808, 2248
Csp6I GTAC 9 cut(s) 61, 121, 449, 512, 1391, 1531, 2709, 2901, 2985
CspAI ACCGGT 1 cut(s) 2478
CspCI CAANNNNNGTGG 2 cut(s) 1998, 2033
CviQI GTAC 9 cut(s) 61, 121, 449, 512, 1391, 1531, 2709, 2901, 2985
DraIII CACNNNGTG 2 cut(s) 2072, 2320
Eam1104I CTCTTC 3 cut(s) 40, 1055, 2877
EarI CTCTTC 3 cut(s) 40, 1055, 2877
Ecl136II GAGCTC 2 cut(s) 394, 1048
Eco130I CCWWGG 2 cut(s) 738, 1432
Eco147I AGGCCT 2 cut(s) 750, 2331
Eco24I GRGCYC 2 cut(s) 396, 1050
Eco32I GATATC 1 cut(s) 1841
Eco47I GGWCC 4 cut(s) 754, 898, 2621, 2802
Eco53kI GAGCTC 2 cut(s) 394, 1048
Eco57I CTGAAG 5 cut(s) 119, 1036, 1523, 2318, 2817
Eco88I CYCGRG 1 cut(s) 1043
EcoICRI GAGCTC 2 cut(s) 394, 1048
EcoO109I RGGNCCY 1 cut(s) 1405
EcoRI GAATTC 1 cut(s) 2826
EcoRII CCWGG 3 cut(s) 750, 2458, 2844
EcoRV GATATC 1 cut(s) 1841
EcoT14I CCWWGG 2 cut(s) 738, 1432
EcoT22I ATGCAT 3 cut(s) 130, 259, 1458
EcoT38I GRGCYC 2 cut(s) 396, 1050
ErhI CCWWGG 2 cut(s) 738, 1432
Esp3I CGTCTC 1 cut(s) 1943
FalI AAGNNNNNCTT 2 cut(s) 1281, 1313
FaqI GGGAC 2 cut(s) 1529, 2607
FbaI TGATCA 1 cut(s) 2713
FblI GTMKAC 1 cut(s) 1597
Fnu4HI GCNGC 6 cut(s) 341, 407, 423, 1200, 1903, 2189
FokI GGATG 5 cut(s) 374, 531, 1465, 1602, 2861
FriOI GRGCYC 2 cut(s) 396, 1050
Fsp4HI GCNGC 6 cut(s) 341, 407, 423, 1200, 1903, 2189
GlaI GCGC 2 cut(s) 98, 2729
GluI GCNGC 6 cut(s) 341, 407, 423, 1200, 1903, 2189
GsuI CTGGAG 1 cut(s) 2007
HaeIII GGCC 4 cut(s) 750, 1406, 2008, 2331
HapII CCGG 3 cut(s) 918, 2479, 2625
HgaI GACGC 2 cut(s) 808, 2248
HhaI GCGC 2 cut(s) 99, 2730
Hin6I GCGC 2 cut(s) 97, 2728
HinP1I GCGC 2 cut(s) 97, 2728
HincII GTYRAC 2 cut(s) 379, 820
HindII GTYRAC 2 cut(s) 379, 820
HindIII AAGCTT 2 cut(s) 1640, 1742
HpaII CCGG 3 cut(s) 918, 2479, 2625
HphI GGTGA 3 cut(s) 98, 338, 2058
Hpy166II GTNNAC 5 cut(s) 379, 820, 1120, 1598, 2109
Hpy8I GTNNAC 5 cut(s) 379, 820, 1120, 1598, 2109
Hpy99I CGWCG 2 cut(s) 567, 2264
HpyAV CCTTC 8 cut(s) 692, 1075, 1145, 1189, 1279, 1629, 2342, 2792
HpyCH4III ACNGT 4 cut(s) 19, 461, 2777, 2974
HpyCH4IV ACGT 2 cut(s) 63, 562
HpySE526I ACGT 2 cut(s) 63, 562
HspAI GCGC 2 cut(s) 97, 2728
Ksp22I TGATCA 1 cut(s) 2713
LguI GCTCTTC 2 cut(s) 1055, 2877
LmnI GCTCC 6 cut(s) 391, 1428, 1673, 1988, 2370, 3010
Lsp1109I GCAGC 5 cut(s) 352, 418, 434, 1186, 1914
LweI GCATC 6 cut(s) 266, 553, 642, 1443, 1927, 2992
MaeII ACGT 2 cut(s) 63, 562
MaeIII GTNAC 8 cut(s) 1211, 1361, 2051, 2064, 2270, 2528, 2645, 2968
MfeI CAATTG 2 cut(s) 210, 893
MflI RGATCY 2 cut(s) 289, 1706
MhlI GDGCHC 4 cut(s) 396, 1050, 1670, 1952
MlyI GAGTC 9 cut(s) 1551, 1638, 1663, 1689, 2048, 2267, 2575, 2624, 2711
MmeI TCCRAC 6 cut(s) 724, 781, 1037, 1606, 1695, 2325
Mph1103I ATGCAT 3 cut(s) 130, 259, 1458
MroXI GAANNNNTTC 2 cut(s) 1064, 1293
MslI CAYNNNNRTG 3 cut(s) 573, 1170, 1451
MspA1I CMGCKG 1 cut(s) 2060
MspI CCGG 3 cut(s) 918, 2479, 2625
MspR9I CCNGG 4 cut(s) 752, 2460, 2625, 2846
MunI CAATTG 2 cut(s) 210, 893
Mva1269I GAATGC 2 cut(s) 1066, 1245
MvaI CCWGG 3 cut(s) 752, 2460, 2846
MvnI CGCG 1 cut(s) 97
NciI CCSGG 1 cut(s) 2625
NcoI CCATGG 2 cut(s) 738, 1432
NheI GCTAGC 1 cut(s) 802
NlaIV GGNNCC 7 cut(s) 291, 308, 1430, 2366, 2623, 2803, 2919
NmuCI GTSAC 6 cut(s) 1361, 2051, 2064, 2270, 2528, 2968
NsiI ATGCAT 3 cut(s) 130, 259, 1458
NspI RCATGY 2 cut(s) 1115, 1311
NspV TTCGAA 1 cut(s) 613
PaeR7I CTCGAG 1 cut(s) 1043
PceI AGGCCT 2 cut(s) 750, 2331
PciSI GCTCTTC 2 cut(s) 1055, 2877
PctI GAATGC 2 cut(s) 1066, 1245
PdmI GAANNNNTTC 2 cut(s) 1064, 1293
PflFI GACNNNGTC 1 cut(s) 1373
PflMI CCANNNNNTGG 1 cut(s) 487
PfoI TCCNGGA 1 cut(s) 2458
PinAI ACCGGT 1 cut(s) 2478
PkrI GCNGC 6 cut(s) 342, 408, 424, 1201, 1904, 2190
PleI GAGTC 9 cut(s) 1550, 1637, 1662, 1689, 2048, 2267, 2575, 2623, 2710
PpsI GAGTC 9 cut(s) 1550, 1637, 1662, 1689, 2048, 2267, 2575, 2623, 2710
PshBI ATTAAT 1 cut(s) 1862
PsiI TTATAA 2 cut(s) 675, 2543
Psp124BI GAGCTC 2 cut(s) 396, 1050
Psp6I CCWGG 3 cut(s) 750, 2458, 2844
PspGI CCWGG 3 cut(s) 750, 2458, 2844
PspN4I GGNNCC 7 cut(s) 291, 308, 1430, 2366, 2623, 2803, 2919
PspPI GGNCC 6 cut(s) 754, 898, 1405, 2006, 2621, 2802
PsuI RGATCY 2 cut(s) 289, 1706
PsyI GACNNNGTC 1 cut(s) 1373
PvuII CAGCTG 1 cut(s) 2060
RsaI GTAC 9 cut(s) 62, 122, 450, 513, 1392, 1532, 2710, 2902, 2986
RsaNI GTAC 9 cut(s) 61, 121, 449, 512, 1391, 1531, 2709, 2901, 2985
RseI CAYNNNNRTG 3 cut(s) 573, 1170, 1451
SacI GAGCTC 2 cut(s) 396, 1050
SapI GCTCTTC 2 cut(s) 1055, 2877
SatI GCNGC 6 cut(s) 341, 407, 423, 1200, 1903, 2189
Sau96I GGNCC 6 cut(s) 754, 898, 1405, 2006, 2621, 2802
ScaI AGTACT 3 cut(s) 1392, 2710, 2902
SchI GAGTC 9 cut(s) 1551, 1638, 1663, 1689, 2048, 2267, 2575, 2624, 2711
ScrFI CCNGG 4 cut(s) 752, 2460, 2625, 2846
SduI GDGCHC 4 cut(s) 396, 1050, 1670, 1952
SfaNI GCATC 6 cut(s) 266, 553, 642, 1443, 1927, 2992
SfcI CTRYAG 3 cut(s) 18, 761, 1799
Sfr274I CTCGAG 1 cut(s) 1043
SfuI TTCGAA 1 cut(s) 613
SinI GGWCC 4 cut(s) 754, 898, 2621, 2802
SlaI CTCGAG 1 cut(s) 1043
SmiMI CAYNNNNRTG 3 cut(s) 573, 1170, 1451
SmlI CTYRAG 7 cut(s) 1043, 1516, 1651, 1676, 1697, 1745, 2641
SmoI CTYRAG 7 cut(s) 1043, 1516, 1651, 1676, 1697, 1745, 2641
SseBI AGGCCT 2 cut(s) 750, 2331
SsiI CCGC 5 cut(s) 304, 520, 686, 2188, 2938
SspI AATATT 3 cut(s) 1860, 2286, 2295
SstI GAGCTC 2 cut(s) 396, 1050
StuI AGGCCT 2 cut(s) 750, 2331
StyD4I CCNGG 4 cut(s) 750, 2458, 2623, 2844
StyI CCWWGG 2 cut(s) 738, 1432
TaaI ACNGT 4 cut(s) 19, 461, 2777, 2974
TaiI ACGT 2 cut(s) 66, 565
TaqII GACCGA 1 cut(s) 493
TatI WGTACW 5 cut(s) 120, 448, 1390, 2708, 2900
TauI GCSGC 1 cut(s) 2191
TscAI CASTG 5 cut(s) 22, 813, 1852, 2283, 2342
TseFI GTSAC 6 cut(s) 1361, 2051, 2064, 2270, 2528, 2968
TseI GCWGC 5 cut(s) 340, 406, 422, 1199, 1902
Tsp45I GTSAC 6 cut(s) 1361, 2051, 2064, 2270, 2528, 2968
TspGWI ACGGA 1 cut(s) 2794
TspRI CASTG 5 cut(s) 22, 813, 1852, 2283, 2342
Tth111I GACNNNGTC 1 cut(s) 1373
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 4 cut(s) 754, 898, 2621, 2802
VspI ATTAAT 1 cut(s) 1862
XapI RAATTY 5 cut(s) 1964, 2223, 2631, 2672, 2826
XbaI TCTAGA 1 cut(s) 1711
XceI RCATGY 2 cut(s) 1115, 1311
XcmI CCANNNNNNNNNTGG 2 cut(s) 2247, 2559
XhoI CTCGAG 1 cut(s) 1043
XmiI GTMKAC 1 cut(s) 1597
XmnI GAANNNNTTC 2 cut(s) 1064, 1293
ZrmI AGTACT 3 cut(s) 1392, 2710, 2902
Zsp2I ATGCAT 3 cut(s) 130, 259, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.