Rh2AG265800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0021553
Physical Location & Seq
Reverse (-)
31022711 .. 31023340
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG265800.1

Sequence Viewer

Length: 630 bp
ATGGATTATTGTGTTACACAGGTGCTATCTTGTCTGGATTTTGGTGTTATACTTTCAGGAAGTGAAGTTCCCCACCGGTTCAACCCTTTGAGGAGTTGCAGGAGTTATGAGATTAAAAATTATTGTGACATTTGCATTATAATACCTCCACCTTTGAATTGGAAAAACGCAAGATTGACTCTATGTGCTGTTATAGGAATAACACAAAATGAGTCTGGTCCCGGTAGAATTTCAGCCTCAAGTTACATCAACCAGCTAATGATCGATGAATTTGAAGAATATATCTATCCCTCAGACGCATCAAGTACTGATCATGTGTGGTTGCGCTATATTTCGTTATCAGGTATTGCTTGTGATGACATGATCCTTAACTGTCAAGCTATATTTTCTTCTGAAGGGTCCGTGGCATTAAAAAGCTGTGGAATTCGTCTAGTATATCCCCAAGATGATGATGATGATGATGAGAATGACGAAGAGCAATGGCTTCCTAGTACTTCTTTGTCTTTGGAACCAATGAATCCTCGAAAGCAGAAACATATAGAGGAGGATCATAACCTCGTCGCAGTCAGCAGTAGTACTGCTAATGTTCATGATGCTCCACAGAATGAGAAGTGGCTTTCGTTGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

209

Amino Acids

23.45

Weight (kDa)

4.44

Isoelectric Point (pI)

53.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021553)

Species Orthologous Gene IDs
rosa_laevigata RLG00000018326
rosa_roxburghii Rroxscaffold_2G00126760 Rroxscaffold_2G00126780
rosa_samantha Rh2AG265800 Rh2DG273100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
AclWI GGATC 2 cut(s) 358, 555
AcsI RAATTY 3 cut(s) 228, 269, 423
AcuI CTGAAG 1 cut(s) 414
AfaI GTAC 3 cut(s) 307, 493, 577
AgeI ACCGGT 1 cut(s) 75
AgsI TTSAA 3 cut(s) 82, 157, 275
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
AluBI AGCT 3 cut(s) 256, 380, 417
AluI AGCT 3 cut(s) 256, 380, 417
AlwI GGATC 2 cut(s) 358, 555
ApoI RAATTY 3 cut(s) 228, 269, 423
AsiGI ACCGGT 1 cut(s) 75
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 2 cut(s) 218, 399
AsuC2I CCSGG 1 cut(s) 222
AvaII GGWCC 2 cut(s) 218, 399
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 1 cut(s) 222
BfaI CTAG 2 cut(s) 431, 489
BmcAI AGTACT 3 cut(s) 307, 493, 577
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 2 cut(s) 218, 399
BmgT120I GGNCC 2 cut(s) 218, 399
BmiI GGNNCC 3 cut(s) 220, 400, 510
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 308, 583
BpuEI CTTGAG 1 cut(s) 223
BpuMI CCSGG 1 cut(s) 222
Bsa29I ATCGAT 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 402
BsaWI WCCGGW 1 cut(s) 75
Bse118I RCCGGY 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 485
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 402
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 106, 557
BshTI ACCGGT 1 cut(s) 75
BshVI ATCGAT 1 cut(s) 264
BsiSI CCGG 2 cut(s) 76, 222
BslFI GGGAC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 204
Bsp143I GATC 4 cut(s) 261, 310, 363, 547
BspCNI CTCAG 1 cut(s) 305
BspDI ATCGAT 1 cut(s) 264
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 3 cut(s) 220, 400, 510
BspPI GGATC 2 cut(s) 358, 555
BspQI GCTCTTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 485
BsrFI RCCGGY 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 4 cut(s) 261, 310, 363, 547
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 1 cut(s) 468
BstDEI CTNAG 1 cut(s) 292
BstDSI CCRYGG 1 cut(s) 402
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 4 cut(s) 264, 313, 366, 550
BstMBI GATC 4 cut(s) 261, 310, 363, 547
BstSCI CCNGG 1 cut(s) 220
Bsu15I ATCGAT 1 cut(s) 264
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 402
CciI TCATGA 1 cut(s) 589
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 218, 399
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 305
Csp6I GTAC 3 cut(s) 306, 492, 576
CspAI ACCGGT 1 cut(s) 75
CviAII CATG 3 cut(s) 314, 361, 590
CviJI RGCY 6 cut(s) 236, 256, 380, 417, 484, 616
CviKI_1 RGCY 6 cut(s) 236, 256, 380, 417, 484, 616
CviQI GTAC 3 cut(s) 306, 492, 576
DdeI CTNAG 1 cut(s) 292
DpnI GATC 4 cut(s) 263, 312, 365, 549
DpnII GATC 4 cut(s) 261, 310, 363, 547
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 218, 399
Eco57I CTGAAG 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 423
FaeI CATG 3 cut(s) 317, 364, 593
FaqI GGGAC 1 cut(s) 204
FatI CATG 3 cut(s) 313, 360, 589
FbaI TGATCA 1 cut(s) 310
FspBI CTAG 2 cut(s) 431, 489
GlaI GCGC 1 cut(s) 326
HapII CCGG 2 cut(s) 76, 222
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 327
Hin1II CATG 3 cut(s) 317, 364, 593
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HinfI GANTC 3 cut(s) 178, 212, 517
HpaII CCGG 2 cut(s) 76, 222
Hpy188I TCNGA 2 cut(s) 295, 394
Hpy188III TCNNGA 3 cut(s) 35, 57, 590
Hpy99I CGWCG 1 cut(s) 563
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4V TGCA 2 cut(s) 99, 135
HpyF3I CTNAG 1 cut(s) 292
Hsp92II CATG 3 cut(s) 317, 364, 593
HspAI GCGC 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 4 cut(s) 261, 310, 363, 547
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 1 cut(s) 601
LpnPI CCDG 9 cut(s) 5, 20, 42, 85, 89, 201, 235, 266, 327
LweI GCATC 2 cut(s) 308, 583
MaeI CTAG 2 cut(s) 431, 489
MaeIII GTNAC 3 cut(s) 13, 125, 242
MalI GATC 4 cut(s) 263, 312, 365, 549
MboI GATC 4 cut(s) 261, 310, 363, 547
MboII GAAGA 3 cut(s) 287, 381, 485
MluCI AATT 5 cut(s) 118, 157, 228, 269, 423
MlyI GAGTC 2 cut(s) 172, 221
MnlI CCTC 8 cut(s) 84, 156, 247, 301, 531, 535, 538, 566
MseI TTAA 3 cut(s) 114, 369, 410
MspI CCGG 2 cut(s) 76, 222
MspR9I CCNGG 1 cut(s) 222
NciI CCSGG 1 cut(s) 222
NdeII GATC 4 cut(s) 261, 310, 363, 547
NlaIII CATG 3 cut(s) 317, 364, 593
NlaIV GGNNCC 3 cut(s) 220, 400, 510
NmuCI GTSAC 1 cut(s) 125
PagI TCATGA 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 468
PfeI GAWTC 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 75
PleI GAGTC 2 cut(s) 172, 220
PpsI GAGTC 2 cut(s) 172, 220
PsiI TTATAA 1 cut(s) 140
PspN4I GGNNCC 3 cut(s) 220, 400, 510
PspPI GGNCC 2 cut(s) 218, 399
RsaI GTAC 3 cut(s) 307, 493, 577
RsaNI GTAC 3 cut(s) 306, 492, 576
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 3 cut(s) 114, 369, 410
Sau3AI GATC 4 cut(s) 261, 310, 363, 547
Sau96I GGNCC 2 cut(s) 218, 399
ScaI AGTACT 3 cut(s) 307, 493, 577
SchI GAGTC 2 cut(s) 172, 221
ScrFI CCNGG 1 cut(s) 222
SetI ASST 8 cut(s) 24, 148, 154, 258, 346, 382, 419, 558
SfaNI GCATC 2 cut(s) 308, 583
SinI GGWCC 2 cut(s) 218, 399
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 5 cut(s) 118, 157, 228, 269, 423
SspMI CTAG 2 cut(s) 431, 489
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 374
TaqI TCGA 2 cut(s) 264, 523
TasI AATT 5 cut(s) 118, 157, 228, 269, 423
TatI WGTACW 3 cut(s) 305, 491, 575
TfiI GAWTC 1 cut(s) 517
Tru1I TTAA 3 cut(s) 114, 369, 410
Tru9I TTAA 3 cut(s) 114, 369, 410
TseFI GTSAC 1 cut(s) 125
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 282, 530, 578
TspGWI ACGGA 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 218, 399
XapI RAATTY 3 cut(s) 228, 269, 423
XcmI CCANNNNNNNNNTGG 1 cut(s) 156
XspI CTAG 2 cut(s) 431, 489
ZrmI AGTACT 3 cut(s) 307, 493, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.