Rroxscaffold_2G00126760

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0021553
Physical Location & Seq
Forward (+)
62134616 .. 62138288
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126760.1

Sequence Viewer

Length: 1500 bp
ATGGACTATGTGAAGCAAATGCTAGAATCTTGTGACCTTGACCCCGTTATTAGTATTGAAGTACTTAAAGAAAAGGCCCTCATAACAATTACTAAAATAGGCAATGGTGTTGAGATCATTTGGATGCATGACTTGTTAGAAGAATTGGGAAAAGAGATAGTGCGCCAAGAATCACCCACCGAGCCAGAAAGCGTAGCGTATTGTGGTTTCATGAAGATAAATTGTGGGGAAGATGGACAATCTGAGATATATGAATCTACAAGGCACTGCCATAACAGGATTGCCTCTCTCTCTGTTGGAGATCTTAGTGGGCTTGAAATGTTAATTCTCGATATTTGTGAAAGCCTTACAACCCTTCCATGCAGCATTTATGAGTTACATCATCTACGGGTTCTTCATCTCTCTAGGTGCTCAAATCTTTTTGCATTTCCTAGTAGAATGAACTATGAAGCATCATCCAGTGCGGTGCTGCTTCCTTTGCAGCTTTCAACTAACCCTTTGGATGATACTTGTGGGTCAGCTGTTTCACATTGTGAACGAGATCACTACAATCACCAAAAAGGCTTGTCCACATTAGAATCACTTGATCTATCAAGAATCAATTTTGTTAGTCTTCCTGCATGGGTTAGAAAGTGTTCTAACTTGTTGCGGCTTAACTTGTGTTGGTGCAAAAGACTTCAAGAAATATCAGAACTTCCACCAAAGATAAGATGGTTGAGCGTCAGTGGTTGTAGCTCACTGGAAATATTGTCAATATTGTCAAACTTGGTGGAACACAACGAGTTGCAAGGCCTTAGGTGGATCGATCTGTCTCATTGCCACAGGCTCCTTGACAATCTGTGCTATGATATAATAAAGATTGATAACGCTATTCGACGAAATCAGGTGCTATCTTGTGTGGATTTTGCTGTTTTACTTCCAGGAAGTAGAGTTCCACAGCGGTTCAACCCTTTGACGAGTCGTAAGAGTGATGATTATGAGACTGAAAATGGTTATGATATTTGGGTTACAACATCTGCACCCTCGAAGTTGGAAAACACAAGATTGACTCTATGTGCTGTTATAGGAATAACACAAAATGAGTCTGGTCCTGGTAGAATTTCAGTTTCAAGTTGCATCAACAATCTAGAGATTGATGAATTTGAAGAATATATTTATCCCACAGACGGGTCAAGTACTGATCATCTATGGCTGCGCTATATTTCATTATCAGGTATTGCTAATGATGATGACAAGAGGGTTATCTCTAAAGTGAGATTTTCTTCTAAAGGGTCCGTGTCATTAAAAAGCTGTGGGATCCGTCTAGTATGCCCCCAGGATGATGATGAGGACGACGATGACGAAGAGCAATGGCTTCCTAGTACTTCCTTGTCTTTGGAGCAAATGAATCCTCGAAAGCGGAAACATGTAGAGGAGGATCATAACCATGTCGTAGTCAGCAGTAGTACTGTTAATGTTGATGCTGCTCCGCAGAAAAAGAAGTGGCTTTCTTTGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

499

Amino Acids

56.32

Weight (kDa)

5.14

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 1 - 58 4.9e-09 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 98 - 258 6.5e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021553)

Species Orthologous Gene IDs
rosa_laevigata RLG00000018326
rosa_roxburghii Rroxscaffold_2G00126760 Rroxscaffold_2G00126780
rosa_samantha Rh2AG265800 Rh2DG273100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 464, 649, 940, 1399, 1469
AclWI GGATC 4 cut(s) 809, 1291, 1304, 1425
AcsI RAATTY 2 cut(s) 1098, 1139
AdeI CACNNNGTG 1 cut(s) 533
AfaI GTAC 4 cut(s) 63, 1177, 1363, 1447
AfiI CCNNNNNNNGG 2 cut(s) 1166, 1167
AflIII ACRYGT 1 cut(s) 1405
AgsI TTSAA 7 cut(s) 59, 317, 489, 680, 946, 1110, 1145
AjnI CCWGG 3 cut(s) 919, 1090, 1314
AloI GAACNNNNNNTCC 2 cut(s) 915, 947
AluBI AGCT 4 cut(s) 484, 521, 735, 1290
AluI AGCT 4 cut(s) 484, 521, 735, 1290
Alw21I GWGCWC 1 cut(s) 413
Alw26I GTCTC 2 cut(s) 816, 974
AlwI GGATC 4 cut(s) 809, 1291, 1304, 1425
AoxI GGCC 2 cut(s) 75, 790
ApeKI GCWGC 5 cut(s) 363, 469, 481, 1192, 1463
ApoI RAATTY 2 cut(s) 1098, 1139
Asp700I GAANNNNTTC 1 cut(s) 634
AspLEI GCGC 2 cut(s) 165, 1197
AspS9I GGNCC 3 cut(s) 76, 1088, 1272
AsuHPI GGTGA 2 cut(s) 165, 545
AvaII GGWCC 2 cut(s) 1088, 1272
AxyI CCTNAGG 1 cut(s) 794
BamHI GGATCC 1 cut(s) 1296
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 413
BbvI GCAGC 5 cut(s) 375, 456, 493, 1179, 1450
BccI CCATC 2 cut(s) 227, 705
BciT130I CCWGG 3 cut(s) 921, 1092, 1316
BclI TGATCA 1 cut(s) 1180
BcoDI GTCTC 2 cut(s) 816, 974
BfaI CTAG 6 cut(s) 23, 405, 432, 1127, 1304, 1359
BglII AGATCT 1 cut(s) 301
BisI GCNGC 6 cut(s) 364, 470, 482, 650, 1193, 1464
BlsI GCNGC 6 cut(s) 365, 471, 483, 651, 1194, 1465
BmcAI AGTACT 4 cut(s) 63, 1177, 1363, 1447
Bme1390I CCNGG 3 cut(s) 921, 1092, 1316
Bme18I GGWCC 2 cut(s) 1088, 1272
BmgT120I GGNCC 3 cut(s) 76, 1088, 1272
BmiI GGNNCC 3 cut(s) 827, 1273, 1298
BmrFI CCNGG 3 cut(s) 921, 1092, 1316
BmsI GCATC 4 cut(s) 114, 461, 1125, 1450
BpiI GAAGAC 1 cut(s) 605
Bsa29I ATCGAT 1 cut(s) 804
BsaJI CCNNGG 1 cut(s) 1314
Bsc4I CCNNNNNNNGG 2 cut(s) 1166, 1167
Bse1I ACTGG 2 cut(s) 459, 744
Bse21I CCTNAGG 1 cut(s) 794
Bse3DI GCAATG 3 cut(s) 109, 814, 1355
BseBI CCWGG 3 cut(s) 921, 1092, 1316
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 1 cut(s) 1314
BseGI GGATG 4 cut(s) 129, 455, 508, 1324
BseLI CCNNNNNNNGG 2 cut(s) 1166, 1167
BseMI GCAATG 3 cut(s) 109, 814, 1355
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 2 cut(s) 459, 744
BseRI GAGGAG 1 cut(s) 1427
BseXI GCAGC 5 cut(s) 375, 456, 493, 1179, 1450
BsgI GTGCAG 1 cut(s) 1002
BshFI GGCC 2 cut(s) 77, 792
BshVI ATCGAT 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 413
BslI CCNNNNNNNGG 2 cut(s) 1166, 1167
BsmAI GTCTC 2 cut(s) 816, 974
BsnI GGCC 2 cut(s) 77, 792
Bsp1286I GDGCHC 1 cut(s) 413
Bsp143I GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
BspACI CCGC 5 cut(s) 464, 649, 940, 1399, 1469
BspANI GGCC 2 cut(s) 77, 792
BspCNI CTCAG 1 cut(s) 235
BspDI ATCGAT 1 cut(s) 804
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 3 cut(s) 827, 1273, 1298
BspPI GGATC 4 cut(s) 809, 1291, 1304, 1425
BspQI GCTCTTC 1 cut(s) 1338
BsrDI GCAATG 3 cut(s) 109, 814, 1355
BsrI ACTGG 2 cut(s) 459, 744
BssECI CCNNGG 1 cut(s) 1314
BssMI GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
Bst2UI CCWGG 3 cut(s) 921, 1092, 1316
Bst4CI ACNGT 1 cut(s) 1450
Bst6I CTCTTC 1 cut(s) 1338
BstDEI CTNAG 3 cut(s) 243, 305, 794
BstF5I GGATG 4 cut(s) 129, 455, 508, 1324
BstHHI GCGC 2 cut(s) 165, 1197
BstKTI GATC 9 cut(s) 117, 304, 544, 589, 804, 808, 1183, 1299, 1420
BstMAI GTCTC 2 cut(s) 816, 974
BstMBI GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
BstMWI GCNNNNNNNGC 1 cut(s) 478
BstNI CCWGG 3 cut(s) 921, 1092, 1316
BstNSI RCATGY 1 cut(s) 1409
BstSCI CCNGG 3 cut(s) 919, 1090, 1314
BstV1I GCAGC 5 cut(s) 375, 456, 493, 1179, 1450
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 301, 1296
BstYI RGATCY 2 cut(s) 301, 1296
Bsu15I ATCGAT 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 794
BsuRI GGCC 2 cut(s) 77, 792
BsuTUI ATCGAT 1 cut(s) 804
BtsCI GGATG 4 cut(s) 129, 455, 508, 1324
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 4 cut(s) 265, 466, 730, 737
CciI TCATGA 1 cut(s) 210
CfoI GCGC 2 cut(s) 165, 1197
Cfr13I GGNCC 3 cut(s) 76, 1088, 1272
ClaI ATCGAT 1 cut(s) 804
CseI GACGC 1 cut(s) 709
Csp6I GTAC 4 cut(s) 62, 1176, 1362, 1446
CspCI CAANNNNNGTGG 2 cut(s) 750, 785
CviAII CATG 6 cut(s) 128, 211, 360, 621, 1406, 1427
CviQI GTAC 4 cut(s) 62, 1176, 1362, 1446
DdeI CTNAG 3 cut(s) 243, 305, 794
DpnI GATC 9 cut(s) 116, 303, 543, 588, 803, 807, 1182, 1298, 1419
DpnII GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
DraIII CACNNNGTG 1 cut(s) 533
Eam1104I CTCTTC 1 cut(s) 1338
EarI CTCTTC 1 cut(s) 1338
Eco147I AGGCCT 1 cut(s) 792
Eco47I GGWCC 2 cut(s) 1088, 1272
Eco81I CCTNAGG 1 cut(s) 794
EcoO109I RGGNCCY 1 cut(s) 76
EcoRII CCWGG 3 cut(s) 919, 1090, 1314
EcoT22I ATGCAT 1 cut(s) 129
FaeI CATG 6 cut(s) 131, 214, 363, 624, 1409, 1430
FatI CATG 6 cut(s) 127, 210, 359, 620, 1405, 1426
FbaI TGATCA 1 cut(s) 1180
Fnu4HI GCNGC 6 cut(s) 364, 470, 482, 650, 1193, 1464
FokI GGATG 4 cut(s) 136, 442, 515, 1331
Fsp4HI GCNGC 6 cut(s) 364, 470, 482, 650, 1193, 1464
FspBI CTAG 6 cut(s) 23, 405, 432, 1127, 1304, 1359
GlaI GCGC 2 cut(s) 164, 1196
GluI GCNGC 6 cut(s) 364, 470, 482, 650, 1193, 1464
HaeIII GGCC 2 cut(s) 77, 792
HgaI GACGC 1 cut(s) 709
HhaI GCGC 2 cut(s) 165, 1197
Hin1II CATG 6 cut(s) 131, 214, 363, 624, 1409, 1430
Hin6I GCGC 2 cut(s) 163, 1195
HinP1I GCGC 2 cut(s) 163, 1195
HinfI GANTC 9 cut(s) 26, 170, 254, 578, 597, 958, 1048, 1082, 1387
HphI GGTGA 2 cut(s) 165, 545
Hpy166II GTNNAC 2 cut(s) 536, 570
Hpy188I TCNGA 2 cut(s) 244, 691
Hpy188III TCNNGA 5 cut(s) 211, 329, 594, 680, 1127
Hpy8I GTNNAC 2 cut(s) 536, 570
Hpy99I CGWCG 2 cut(s) 879, 1337
HpyAV CCTTC 1 cut(s) 365
HpyCH4III ACNGT 1 cut(s) 1450
HpyCH4V TGCA 9 cut(s) 127, 363, 425, 481, 620, 669, 787, 1019, 1116
HpyF10VI GCNNNNNNNGC 1 cut(s) 478
HpyF3I CTNAG 3 cut(s) 243, 305, 794
Hsp92II CATG 6 cut(s) 131, 214, 363, 624, 1409, 1430
HspAI GCGC 2 cut(s) 163, 1195
Ksp22I TGATCA 1 cut(s) 1180
Kzo9I GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
LguI GCTCTTC 1 cut(s) 1338
LmnI GCTCC 3 cut(s) 831, 1378, 1471
Lsp1109I GCAGC 5 cut(s) 375, 456, 493, 1179, 1450
LweI GCATC 4 cut(s) 114, 461, 1125, 1450
MaeI CTAG 6 cut(s) 23, 405, 432, 1127, 1304, 1359
MaeIII GTNAC 3 cut(s) 32, 375, 1006
MalI GATC 9 cut(s) 116, 303, 543, 588, 803, 807, 1182, 1298, 1419
MboI GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
MboII GAAGA 8 cut(s) 152, 226, 242, 386, 605, 1157, 1254, 1355
MflI RGATCY 2 cut(s) 301, 1296
MhlI GDGCHC 1 cut(s) 413
MluCI AATT 7 cut(s) 87, 143, 220, 324, 601, 1098, 1139
MlyI GAGTC 3 cut(s) 967, 1042, 1091
MmeI TCCRAC 2 cut(s) 277, 1011
MnlI CCTC 8 cut(s) 89, 295, 1033, 1230, 1321, 1401, 1405, 1408
Mph1103I ATGCAT 1 cut(s) 129
MroXI GAANNNNTTC 1 cut(s) 634
MseI TTAA 5 cut(s) 66, 323, 654, 1283, 1452
MslI CAYNNNNRTG 2 cut(s) 122, 1425
MspA1I CMGCKG 2 cut(s) 521, 940
MspR9I CCNGG 3 cut(s) 921, 1092, 1316
MvaI CCWGG 3 cut(s) 921, 1092, 1316
MwoI GCNNNNNNNGC 1 cut(s) 478
NdeII GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
NlaIII CATG 6 cut(s) 131, 214, 363, 624, 1409, 1430
NlaIV GGNNCC 3 cut(s) 827, 1273, 1298
NmuCI GTSAC 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 129
NspI RCATGY 1 cut(s) 1409
PagI TCATGA 1 cut(s) 210
PceI AGGCCT 1 cut(s) 792
PciI ACATGT 1 cut(s) 1405
PciSI GCTCTTC 1 cut(s) 1338
PcsI WCGNNNNNNNCGW 1 cut(s) 1338
PdmI GAANNNNTTC 1 cut(s) 634
PfeI GAWTC 6 cut(s) 26, 170, 254, 578, 597, 1387
PfoI TCCNGGA 1 cut(s) 919
PkrI GCNGC 6 cut(s) 365, 471, 483, 651, 1194, 1465
PleI GAGTC 3 cut(s) 966, 1042, 1090
PpsI GAGTC 3 cut(s) 966, 1042, 1090
PscI ACATGT 1 cut(s) 1405
Psp6I CCWGG 3 cut(s) 919, 1090, 1314
PspGI CCWGG 3 cut(s) 919, 1090, 1314
PspN4I GGNNCC 3 cut(s) 827, 1273, 1298
PspPI GGNCC 3 cut(s) 76, 1088, 1272
PsuI RGATCY 2 cut(s) 301, 1296
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 4 cut(s) 63, 1177, 1363, 1447
RsaNI GTAC 4 cut(s) 62, 1176, 1362, 1446
RseI CAYNNNNRTG 2 cut(s) 122, 1425
SapI GCTCTTC 1 cut(s) 1338
SaqAI TTAA 5 cut(s) 66, 323, 654, 1283, 1452
SatI GCNGC 6 cut(s) 364, 470, 482, 650, 1193, 1464
Sau3AI GATC 9 cut(s) 114, 301, 541, 586, 801, 805, 1180, 1296, 1417
Sau96I GGNCC 3 cut(s) 76, 1088, 1272
ScaI AGTACT 4 cut(s) 63, 1177, 1363, 1447
SchI GAGTC 3 cut(s) 967, 1042, 1091
ScrFI CCNGG 3 cut(s) 921, 1092, 1316
SduI GDGCHC 1 cut(s) 413
SetI ASST 9 cut(s) 39, 410, 486, 523, 737, 800, 888, 1216, 1292
SfaNI GCATC 4 cut(s) 114, 461, 1125, 1450
SinI GGWCC 2 cut(s) 1088, 1272
SmiMI CAYNNNNRTG 2 cut(s) 122, 1425
Sse9I AATT 7 cut(s) 87, 143, 220, 324, 601, 1098, 1139
SseBI AGGCCT 1 cut(s) 792
SsiI CCGC 5 cut(s) 464, 649, 940, 1399, 1469
SspI AATATT 2 cut(s) 747, 756
SspMI CTAG 6 cut(s) 23, 405, 432, 1127, 1304, 1359
StuI AGGCCT 1 cut(s) 792
StyD4I CCNGG 3 cut(s) 919, 1090, 1314
TaaI ACNGT 1 cut(s) 1450
TaqI TCGA 5 cut(s) 330, 804, 874, 1025, 1393
TasI AATT 7 cut(s) 87, 143, 220, 324, 601, 1098, 1139
TatI WGTACW 4 cut(s) 61, 1175, 1361, 1445
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 6 cut(s) 26, 170, 254, 578, 597, 1387
Tru1I TTAA 5 cut(s) 66, 323, 654, 1283, 1452
Tru9I TTAA 5 cut(s) 66, 323, 654, 1283, 1452
TscAI CASTG 4 cut(s) 272, 466, 730, 744
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 5 cut(s) 363, 469, 481, 1192, 1463
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 9 cut(s) 199, 227, 267, 386, 455, 462, 1152, 1194, 1400
TspGWI ACGGA 2 cut(s) 1264, 1289
TspRI CASTG 4 cut(s) 272, 466, 730, 744
VpaK11BI GGWCC 2 cut(s) 1088, 1272
XapI RAATTY 2 cut(s) 1098, 1139
XbaI TCTAGA 1 cut(s) 1126
XceI RCATGY 1 cut(s) 1409
XcmI CCANNNNNNNNNTGG 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 634
XspI CTAG 6 cut(s) 23, 405, 432, 1127, 1304, 1359
ZrmI AGTACT 4 cut(s) 63, 1177, 1363, 1447
Zsp2I ATGCAT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.