RLG00000019627

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019887
Physical Location & Seq
Reverse (-)
54473230 .. 54474507
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019627

Sequence Viewer

Length: 396 bp
ATGGGAGCATATGTCGAAGCCCCGCAAGCTGTTACAGAAGATGCAGGTCCTTGTCAGTGTGGGCCCTATAAAGTAGACTTTGGGATTTTGGGGTTTTCAGCAAGACGACGCAATCTCACACTAGCTCACTCTCTTTTTAACTCTACAATTGTGCATATATGGCATGATTGCTCAGACCCAGGAATTGCTCCTTGCCGCCCATCTCAAGTAGCAAACCATCGATGTATGTCTTGTTTCAAATCAGATATTTTTACCGCTAGTGATGGAAAGATGAGGCTAAAGGCGATGTTCAACTCACATCTAAGTTCTTATGCTATCTCTGGGAGTATTCAGAACCTGGGCCTCATTCGTCTTCTTGATTACACTATTAATGGCATTCCAAACATGCCCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

132

Amino Acids

14.32

Weight (kDa)

7.65

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 84 - 127 5.3e-09 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019887)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0106871 RchiOBHm_Chr4g0402291 RchiOBHm_Chr5g0032071 RchiOBHm_Chr5g0038981
rosa_laevigata RLG00000019627
rosa_samantha Rh2BG201800 Rh5BG223600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 35
AccI GTMKAC 1 cut(s) 75
AciI CCGC 3 cut(s) 23, 196, 255
AgsI TTSAA 2 cut(s) 238, 292
AjnI CCWGG 2 cut(s) 178, 336
AluBI AGCT 2 cut(s) 29, 125
AluI AGCT 2 cut(s) 29, 125
AlwNI CAGNNNCTG 1 cut(s) 337
AoxI GGCC 2 cut(s) 62, 340
ApaI GGGCCC 1 cut(s) 66
AseI ATTAAT 1 cut(s) 369
AspS9I GGNCC 4 cut(s) 47, 62, 63, 340
AvaII GGWCC 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 344
BccI CCATC 3 cut(s) 208, 225, 257
BciT130I CCWGG 2 cut(s) 180, 338
BfaI CTAG 2 cut(s) 122, 258
BfuAI ACCTGC 1 cut(s) 35
BisI GCNGC 1 cut(s) 196
BlsI GCNGC 1 cut(s) 197
Bme1390I CCNGG 2 cut(s) 180, 338
Bme18I GGWCC 1 cut(s) 47
BmgT120I GGNCC 4 cut(s) 47, 62, 63, 340
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 2 cut(s) 180, 338
BmsI GCATC 1 cut(s) 31
BpiI GAAGAC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 178, 337
BsaXI ACNNNNNCTCC 1 cut(s) 27
BseBI CCWGG 2 cut(s) 180, 338
BseCI ATCGAT 1 cut(s) 220
BseDI CCNNGG 2 cut(s) 178, 337
BseMII CTCAG 1 cut(s) 186
BseSI GKGCMC 1 cut(s) 66
BshFI GGCC 2 cut(s) 64, 342
BshVI ATCGAT 1 cut(s) 220
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 2 cut(s) 64, 342
Bsp120I GGGCCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 66
BspACI CCGC 3 cut(s) 23, 196, 255
BspANI GGCC 2 cut(s) 64, 342
BspCNI CTCAG 1 cut(s) 185
BspDI ATCGAT 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 64
BspMI ACCTGC 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 178, 337
Bst2UI CCWGG 2 cut(s) 180, 338
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 172, 302
BstMWI GCNNNNNNNGC 2 cut(s) 26, 160
BstNI CCWGG 2 cut(s) 180, 338
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 178, 336
BstSLI GKGCMC 1 cut(s) 66
BstV2I GAAGAC 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 220
BsuRI GGCC 2 cut(s) 64, 342
BsuTUI ATCGAT 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 299
BtsIMutI CAGTG 1 cut(s) 62
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 27
CaiI CAGNNNCTG 1 cut(s) 337
Cfr13I GGNCC 4 cut(s) 47, 62, 63, 340
ClaI ATCGAT 1 cut(s) 220
CseI GACGC 1 cut(s) 117
CviAII CATG 2 cut(s) 164, 385
CviJI RGCY 6 cut(s) 20, 29, 64, 125, 277, 342
CviKI_1 RGCY 6 cut(s) 20, 29, 64, 125, 277, 342
DdeI CTNAG 2 cut(s) 172, 302
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 47
EcoO109I RGGNCCY 2 cut(s) 47, 63
EcoRII CCWGG 2 cut(s) 178, 336
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 2 cut(s) 167, 388
FatI CATG 2 cut(s) 163, 384
FauI CCCGC 1 cut(s) 30
FauNDI CATATG 1 cut(s) 10
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 1 cut(s) 196
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 196
FspBI CTAG 2 cut(s) 122, 258
GluI GCNGC 1 cut(s) 196
HaeIII GGCC 2 cut(s) 64, 342
HgaI GACGC 1 cut(s) 117
Hin1II CATG 2 cut(s) 167, 388
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 3 cut(s) 175, 244, 333
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 111
HpyCH4V TGCA 2 cut(s) 44, 154
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 160
HpyF3I CTNAG 2 cut(s) 172, 302
Hsp92II CATG 2 cut(s) 167, 388
LmnI GCTCC 2 cut(s) 5, 193
LpnPI CCDG 6 cut(s) 30, 165, 192, 306, 323, 350
LweI GCATC 1 cut(s) 31
MaeI CTAG 2 cut(s) 122, 258
MaeIII GTNAC 1 cut(s) 31
MboII GAAGA 2 cut(s) 50, 344
MfeI CAATTG 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 2 cut(s) 147, 183
MnlI CCTC 2 cut(s) 267, 353
MseI TTAA 2 cut(s) 138, 369
MspR9I CCNGG 2 cut(s) 180, 338
MunI CAATTG 1 cut(s) 147
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 2 cut(s) 180, 338
MwoI GCNNNNNNNGC 2 cut(s) 26, 160
NdeI CATATG 1 cut(s) 10
NlaIII CATG 2 cut(s) 167, 388
NlaIV GGNNCC 1 cut(s) 64
NspI RCATGY 1 cut(s) 388
PctI GAATGC 1 cut(s) 375
PkrI GCNGC 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 47
PshBI ATTAAT 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 2 cut(s) 178, 336
PspGI CCWGG 2 cut(s) 178, 336
PspN4I GGNNCC 1 cut(s) 64
PspOMI GGGCCC 1 cut(s) 62
PspPI GGNCC 4 cut(s) 47, 62, 63, 340
PspPPI RGGWCCY 1 cut(s) 47
PstNI CAGNNNCTG 1 cut(s) 337
SaqAI TTAA 2 cut(s) 138, 369
SatI GCNGC 1 cut(s) 196
Sau96I GGNCC 4 cut(s) 47, 62, 63, 340
ScrFI CCNGG 2 cut(s) 180, 338
SduI GDGCHC 1 cut(s) 66
SetI ASST 4 cut(s) 31, 49, 127, 339
SfaNI GCATC 1 cut(s) 31
SinI GGWCC 1 cut(s) 47
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
Sse9I AATT 2 cut(s) 147, 183
SsiI CCGC 3 cut(s) 23, 196, 255
SspMI CTAG 2 cut(s) 122, 258
StyD4I CCNGG 2 cut(s) 178, 336
TaqI TCGA 2 cut(s) 15, 220
TasI AATT 2 cut(s) 147, 183
TauI GCSGC 1 cut(s) 198
Tru1I TTAA 2 cut(s) 138, 369
Tru9I TTAA 2 cut(s) 138, 369
TscAI CASTG 1 cut(s) 62
TspRI CASTG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 47
VspI ATTAAT 1 cut(s) 369
XceI RCATGY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 2 cut(s) 122, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.