Rh2BG201800

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0019887
Physical Location & Seq
Reverse (-)
18587589 .. 18589064
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG201800.1

Sequence Viewer

Length: 435 bp
ATGTTCAACTCACATCTGAGTTCTTATGTTATCTCTGGGAGTATTCAGAACCTGGGCCTCATTCGTCTTCTTGATTACACTGTTAATGACATTCCAAACATGCCCGATAATGGTCTGCTAAAGGAAGTTGAGACTTTTACCAAAAGCCTTACACAAGCCTCGGAAGATTTGAGATGGCTATTCTCTTCAAGTTCTAGTGTAATTTATAGATCCACCATTCAACATTTACAAGCTCCCATATTCCTTAACAAGGGAACCATGCGTACTTACACGAATGCTAGAGGTCAAGGCCGTGTCTTCAATGTGGAGTTTACAGACGAAGATGGAACTAAAATTCAAGCTACAATGTTTAATGAAGCTGCAAGGAAGTTTTTTGAAAAGTTTCAACTAAGGCCGTGTTTTCAATGTGGAGTTAATAGACGAAGATGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

144

Amino Acids

16.61

Weight (kDa)

9.3

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019887)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0106871 RchiOBHm_Chr4g0402291 RchiOBHm_Chr5g0032071 RchiOBHm_Chr5g0038981
rosa_laevigata RLG00000019627
rosa_samantha Rh2BG201800 Rh5BG223600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 2 cut(s) 110, 250
AgsI TTSAA 8 cut(s) 7, 189, 221, 301, 338, 377, 386, 404
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 3 cut(s) 233, 341, 359
AluI AGCT 3 cut(s) 233, 341, 359
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 1 cut(s) 204
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 3 cut(s) 55, 289, 392
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 381
AspS9I GGNCC 1 cut(s) 55
BbsI GAAGAC 2 cut(s) 59, 289
BbvI GCAGC 1 cut(s) 346
BccI CCATC 3 cut(s) 168, 317, 420
BceAI ACGGC 2 cut(s) 276, 379
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 2 cut(s) 195, 279
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 53
BpiI GAAGAC 2 cut(s) 59, 289
BsaJI CCNNGG 2 cut(s) 52, 159
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 250
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 52, 159
BseLI CCNNNNNNNGG 2 cut(s) 110, 250
BseMII CTCAG 1 cut(s) 8
BseXI GCAGC 1 cut(s) 346
BshFI GGCC 3 cut(s) 57, 291, 394
BslI CCNNNNNNNGG 2 cut(s) 110, 250
BsmAI GTCTC 1 cut(s) 125
BsmI GAATGC 1 cut(s) 280
BsnI GGCC 3 cut(s) 57, 291, 394
Bsp143I GATC 1 cut(s) 209
BspANI GGCC 3 cut(s) 57, 291, 394
BspCNI CTCAG 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 52, 159
BssMI GATC 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 190
BstDEI CTNAG 2 cut(s) 17, 389
BstENI CCTNNNNNAGG 1 cut(s) 248
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 1 cut(s) 209
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 346
BstV2I GAAGAC 2 cut(s) 59, 289
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BsuRI GGCC 3 cut(s) 57, 291, 394
BtsIMutI CAGTG 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 55
Csp6I GTAC 1 cut(s) 264
CviAII CATG 2 cut(s) 100, 259
CviJI RGCY 9 cut(s) 57, 147, 158, 178, 233, 291, 341, 359, 394
CviKI_1 RGCY 9 cut(s) 57, 147, 158, 178, 233, 291, 341, 359, 394
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 2 cut(s) 17, 389
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 103, 262
FaiI YATR 5 cut(s) 27, 101, 207, 239, 260
FatI CATG 2 cut(s) 99, 258
Fnu4HI GCNGC 1 cut(s) 360
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 2 cut(s) 195, 279
GluI GCNGC 1 cut(s) 360
HaeIII GGCC 3 cut(s) 57, 291, 394
Hin1II CATG 2 cut(s) 103, 262
Hpy166II GTNNAC 1 cut(s) 312
Hpy188I TCNGA 3 cut(s) 18, 48, 163
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 1 cut(s) 312
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4V TGCA 1 cut(s) 362
HpyF3I CTNAG 2 cut(s) 17, 389
Hsp92II CATG 2 cut(s) 103, 262
Kzo9I GATC 1 cut(s) 209
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 3 cut(s) 21, 38, 65
Lsp1109I GCAGC 1 cut(s) 346
MaeI CTAG 2 cut(s) 195, 279
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MboII GAAGA 6 cut(s) 59, 176, 177, 289, 332, 435
MflI RGATCY 1 cut(s) 209
MluCI AATT 2 cut(s) 201, 333
MnlI CCTC 3 cut(s) 68, 169, 275
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 4 cut(s) 84, 246, 351, 414
MspR9I CCNGG 1 cut(s) 53
Mva1269I GAATGC 1 cut(s) 280
MvaI CCWGG 1 cut(s) 53
NdeII GATC 1 cut(s) 209
NlaIII CATG 2 cut(s) 103, 262
NlaIV GGNNCC 1 cut(s) 256
NspI RCATGY 1 cut(s) 103
PctI GAATGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 381
PkrI GCNGC 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 55
PsrI GAACNNNNNNTAC 2 cut(s) 247, 279
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 1 cut(s) 209
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 4 cut(s) 84, 246, 351, 414
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 1 cut(s) 55
ScrFI CCNGG 1 cut(s) 53
SetI ASST 5 cut(s) 54, 235, 286, 343, 361
Sse9I AATT 2 cut(s) 201, 333
SspMI CTAG 2 cut(s) 195, 279
StyD4I CCNGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 82
TasI AATT 2 cut(s) 201, 333
Tru1I TTAA 4 cut(s) 84, 246, 351, 414
Tru9I TTAA 4 cut(s) 84, 246, 351, 414
TscAI CASTG 1 cut(s) 85
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 1 cut(s) 369
TspRI CASTG 1 cut(s) 85
XagI CCTNNNNNAGG 1 cut(s) 248
XapI RAATTY 1 cut(s) 333
XceI RCATGY 1 cut(s) 103
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 2 cut(s) 195, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.