RLG00000020030

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016488
Physical Location & Seq
Forward (+)
60321416 .. 60322344
929 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020030

Sequence Viewer

Length: 612 bp
ATGGCTAATTCTTTGTTTTCAGCAGCCTTCGAAGTGCTGGGTAATGGAACTGAAGGTGGATCTTGTGGATATGGGGATCCCTTCGCACAAGGATATGGGATTGCGACGACAGCTCTGAGCACTGCGCTGTACAATGGCGGCAAGACCTGCGGTGCTTGTTTTGAGAGTGCGCGGATGATAAATGGTGTCAGTCTGGTACCATCAAACCCTCCTCTGAAACACTTTGATTTGACCATGCCAATGTTCTTAAGGATAGCGATAGCCCAACAAGCTGAGACTGTTCCGATACGGTTTCGAAGGAGCTGGTGCAGTAGGAGAGGAGGGATTAGGTTTACGCTTGAGGGTAACCCGAACTTCCTATTACTTGTGTTGGTGTCTATTAATGTTGGAGGCGATGGTGATGTGGTTGATGTCAAAATCAAGGGCTCAAGGGAACCAACTTGGTGGCAAATGAAGCGTGACTGGGGACAAAAATGGCGGACTGAAGCATACTTGGTAGGGCAAAGCTTGTCGTTCCAAGTGACTATTAGTGATGGGAGCTCGCTTTGCTCCGAGAGTGCTGCCCCTGACAATTGGGAATTTGGTCAAACGTATGCGAGAAAAAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

204

Amino Acids

22.17

Weight (kDa)

8.29

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Expansin_C PF01357 109 - 189 4.9e-22 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56320 AT5G56320
fragaria_vesca FvH4_3g45970 FvH4_6g33460
rosa_chinensis RchiOBHm_Chr5g0084081
rosa_laevigata RLG00000020030 RLG00000037080
rosa_multiflora Rmu_ssc0000372.1_g000092
rosa_roxburghii Rroxscaffold_1G00000560
rosa_rugosa Rorug05G0489000
rosa_samantha Rh5BG567100 Rh5CG588900 Rh5DG575300
rosa_wichuraiana Rw5G050370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 155
Acc65I GGTACC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 196
AccII CGCG 1 cut(s) 172
AciI CCGC 4 cut(s) 138, 150, 172, 478
AclWI GGATC 3 cut(s) 67, 71, 84
AcsI RAATTY 1 cut(s) 578
AcuI CTGAAG 2 cut(s) 72, 504
AfaI GTAC 2 cut(s) 131, 198
AflII CTTAAG 1 cut(s) 247
AluBI AGCT 5 cut(s) 113, 272, 303, 507, 540
AluI AGCT 5 cut(s) 113, 272, 303, 507, 540
Alw21I GWGCWC 2 cut(s) 122, 542
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 67, 71, 84
ApeKI GCWGC 2 cut(s) 23, 560
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 381
Asp718I GGTACC 1 cut(s) 196
AspLEI GCGC 2 cut(s) 127, 172
AsuHPI GGTGA 1 cut(s) 410
AsuII TTCGAA 2 cut(s) 30, 295
BamHI GGATCC 1 cut(s) 76
BanI GGYRCC 1 cut(s) 196
BanII GRGCYC 2 cut(s) 428, 542
Bbv12I GWGCWC 2 cut(s) 122, 542
BbvI GCAGC 2 cut(s) 35, 547
BccI CCATC 3 cut(s) 208, 389, 527
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BcoDI GTCTC 1 cut(s) 269
BfrI CTTAAG 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 155
BisI GCNGC 3 cut(s) 24, 139, 561
BlsI GCNGC 3 cut(s) 25, 140, 562
BmiI GGNNCC 3 cut(s) 78, 198, 435
BmrI ACTGGG 1 cut(s) 472
BmuI ACTGGG 1 cut(s) 472
Bpu14I TTCGAA 2 cut(s) 30, 295
BpuEI CTTGAG 2 cut(s) 359, 412
Bse1I ACTGG 1 cut(s) 467
BseGI GGATG 1 cut(s) 180
BseMII CTCAG 2 cut(s) 107, 264
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 2 cut(s) 201, 333
BseXI GCAGC 2 cut(s) 35, 547
BseYI CCCAGC 1 cut(s) 37
BsgI GTGCAG 1 cut(s) 328
Bsh1236I CGCG 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 196
BsiHKAI GWGCWC 2 cut(s) 122, 542
BslFI GGGAC 1 cut(s) 480
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 480
Bsp119I TTCGAA 2 cut(s) 30, 295
Bsp1286I GDGCHC 3 cut(s) 122, 428, 542
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 2 cut(s) 59, 76
BspACI CCGC 4 cut(s) 138, 150, 172, 478
BspCNI CTCAG 2 cut(s) 108, 265
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 3 cut(s) 78, 198, 435
BspMI ACCTGC 1 cut(s) 155
BspPI GGATC 3 cut(s) 67, 71, 84
BspT104I TTCGAA 2 cut(s) 30, 295
BspT107I GGYRCC 1 cut(s) 196
BspTI CTTAAG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 1 cut(s) 467
BssMI GATC 2 cut(s) 59, 76
Bst4CI ACNGT 2 cut(s) 280, 291
BstAFI CTTAAG 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 129
BstBI TTCGAA 2 cut(s) 30, 295
BstC8I GCNNGC 1 cut(s) 542
BstDEI CTNAG 2 cut(s) 116, 273
BstEII GGTNACC 1 cut(s) 344
BstF5I GGATG 1 cut(s) 180
BstFNI CGCG 1 cut(s) 172
BstHHI GCGC 2 cut(s) 127, 172
BstKTI GATC 2 cut(s) 62, 79
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 2 cut(s) 59, 76
BstMWI GCNNNNNNNGC 5 cut(s) 110, 147, 269, 454, 546
BstPI GGTNACC 1 cut(s) 344
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 35, 547
BstX2I RGATCY 2 cut(s) 59, 76
BstXI CCANNNNNNTGG 1 cut(s) 444
BstYI RGATCY 2 cut(s) 59, 76
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 1 cut(s) 542
CfoI GCGC 2 cut(s) 127, 172
Csp6I GTAC 2 cut(s) 130, 197
CviAII CATG 1 cut(s) 235
CviJI RGCY 9 cut(s) 5, 26, 113, 263, 272, 303, 426, 507, 540
CviKI_1 RGCY 9 cut(s) 5, 26, 113, 263, 272, 303, 426, 507, 540
CviQI GTAC 2 cut(s) 130, 197
DdeI CTNAG 2 cut(s) 116, 273
DpnI GATC 2 cut(s) 61, 78
DpnII GATC 2 cut(s) 59, 76
EciI GGCGGA 1 cut(s) 493
Ecl136II GAGCTC 1 cut(s) 540
Eco24I GRGCYC 2 cut(s) 428, 542
Eco53kI GAGCTC 1 cut(s) 540
Eco57I CTGAAG 2 cut(s) 72, 504
Eco91I GGTNACC 1 cut(s) 344
EcoICRI GAGCTC 1 cut(s) 540
EcoO65I GGTNACC 1 cut(s) 344
EcoT38I GRGCYC 2 cut(s) 428, 542
FaeI CATG 1 cut(s) 238
FaiI YATR 5 cut(s) 72, 96, 236, 490, 594
FaqI GGGAC 1 cut(s) 480
FatI CATG 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 24, 139, 561
FokI GGATG 1 cut(s) 187
FriOI GRGCYC 2 cut(s) 428, 542
Fsp4HI GCNGC 3 cut(s) 24, 139, 561
GlaI GCGC 2 cut(s) 126, 171
GluI GCNGC 3 cut(s) 24, 139, 561
GsaI CCCAGC 1 cut(s) 41
HhaI GCGC 2 cut(s) 127, 172
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 2 cut(s) 125, 170
HinP1I GCGC 2 cut(s) 125, 170
HindIII AAGCTT 1 cut(s) 505
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 4 cut(s) 117, 216, 285, 553
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 4 cut(s) 37, 47, 91, 291
HpyCH4III ACNGT 2 cut(s) 280, 291
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 147, 269, 454, 546
HpyF3I CTNAG 2 cut(s) 116, 273
HpySE526I ACGT 1 cut(s) 590
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 2 cut(s) 125, 170
KpnI GGTACC 1 cut(s) 200
Kzo9I GATC 2 cut(s) 59, 76
LmnI GCTCC 3 cut(s) 300, 537, 554
LpnPI CCDG 6 cut(s) 23, 160, 179, 289, 448, 579
Lsp1109I GCAGC 2 cut(s) 35, 547
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 3 cut(s) 344, 458, 520
MalI GATC 2 cut(s) 61, 78
MboI GATC 2 cut(s) 59, 76
MfeI CAATTG 1 cut(s) 571
MflI RGATCY 2 cut(s) 59, 76
MhlI GDGCHC 3 cut(s) 122, 428, 542
MluCI AATT 3 cut(s) 7, 571, 578
MmeI TCCRAC 1 cut(s) 367
MnlI CCTC 6 cut(s) 219, 222, 311, 314, 334, 383
MseI TTAA 2 cut(s) 248, 381
MslI CAYNNNNRTG 1 cut(s) 239
MspCI CTTAAG 1 cut(s) 247
MunI CAATTG 1 cut(s) 571
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 5 cut(s) 110, 147, 269, 454, 546
NdeII GATC 2 cut(s) 59, 76
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 3 cut(s) 78, 198, 435
NmuCI GTSAC 2 cut(s) 458, 520
NspV TTCGAA 2 cut(s) 30, 295
PkrI GCNGC 3 cut(s) 25, 140, 562
PshBI ATTAAT 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 542
PspEI GGTNACC 1 cut(s) 344
PspFI CCCAGC 1 cut(s) 37
PspN4I GGNNCC 3 cut(s) 78, 198, 435
PsuI RGATCY 2 cut(s) 59, 76
RsaI GTAC 2 cut(s) 131, 198
RsaNI GTAC 2 cut(s) 130, 197
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 542
SaqAI TTAA 2 cut(s) 248, 381
SatI GCNGC 3 cut(s) 24, 139, 561
Sau3AI GATC 2 cut(s) 59, 76
SduI GDGCHC 3 cut(s) 122, 428, 542
SetI ASST 9 cut(s) 58, 115, 149, 274, 305, 332, 509, 542, 593
SfuI TTCGAA 2 cut(s) 30, 295
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 3 cut(s) 247, 338, 427
SmoI CTYRAG 3 cut(s) 247, 338, 427
Sse9I AATT 3 cut(s) 7, 571, 578
SsiI CCGC 4 cut(s) 138, 150, 172, 478
SstI GAGCTC 1 cut(s) 542
TaaI ACNGT 2 cut(s) 280, 291
TaiI ACGT 1 cut(s) 593
TaqI TCGA 2 cut(s) 30, 295
TasI AATT 3 cut(s) 7, 571, 578
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 141
Tru1I TTAA 2 cut(s) 248, 381
Tru9I TTAA 2 cut(s) 248, 381
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 2 cut(s) 458, 520
TseI GCWGC 2 cut(s) 23, 560
Tsp45I GTSAC 2 cut(s) 458, 520
TspDTI ATGAA 1 cut(s) 467
TspRI CASTG 1 cut(s) 127
Vha464I CTTAAG 1 cut(s) 247
VspI ATTAAT 1 cut(s) 381
XapI RAATTY 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.