RLG00000020658

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020656
Physical Location & Seq
Reverse (-)
67222870 .. 67223699
830 bp
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UTR
Exon/CDS
Intron
RLM00000020658

Sequence Viewer

Length: 477 bp
ATGCTTGCCCGACTGGAGCTTTCCCATGAAGTTCGGACTGATAACCTGTCGTTGTTTGGCATCACCGAGAAGGAGGATCCGAGAAGAGTAACCACAATGCAAGTTCATGATCATACAGCAGTAATCATTACGTTCGATCTTATTTATATGCTGCGTAAGTACACCTGCAGCATCATCATCAGAATTAGCACCACCGCCAATGGCAAAGCCAAATTGCATGTACAACGCATTGCATGCCGAGGTGTTCCAGTCCCATACTCATTCGGAATCGCACCTCATAGATATTGCTACTTTGACGAGTGGTTTCATATTGACTGTAACGAGTCTACAGGGAACAAGTCTTTCTTGAGGCTCACTCAACTGGAGGTCCTGAATATTTCTAACGAAGGCAAGCTTCCGGTGAAAAGCCCTGTTACTTTCTTAAAGTGCAAGGGAAAACAAACTCGCCAAGCTGCACTAGACCGGAAGCCCTTTTGA

Protein Analysis

159

Amino Acids

18.17

Weight (kDa)

9.42

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 78 - 130 3.8e-07 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020656)

Species Orthologous Gene IDs
rosa_laevigata RLG00000020658
rosa_multiflora Rmu_ssc0000489.1_g000005
rosa_samantha Rh2AG498200 Rh2BG509400 Rh2CG484300 Rh2DG521100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 326
AciI CCGC 1 cut(s) 195
AclWI GGATC 2 cut(s) 71, 84
AfaI GTAC 2 cut(s) 161, 222
AluBI AGCT 3 cut(s) 19, 394, 452
AluI AGCT 3 cut(s) 19, 394, 452
AlwI GGATC 2 cut(s) 71, 84
ApeKI GCWGC 3 cut(s) 151, 168, 452
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 2 cut(s) 55, 412
AvaII GGWCC 1 cut(s) 367
BamHI GGATCC 1 cut(s) 76
BbvI GCAGC 3 cut(s) 138, 180, 439
BclI TGATCA 1 cut(s) 109
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 2 cut(s) 166, 327
BfuAI ACCTGC 1 cut(s) 173
BisI GCNGC 3 cut(s) 152, 169, 453
BlsI GCNGC 3 cut(s) 153, 170, 454
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 1 cut(s) 78
BmsI GCATC 2 cut(s) 69, 180
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 2 cut(s) 35, 383
BpuEI CTTGAG 1 cut(s) 367
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 2 cut(s) 397, 462
Bse1I ACTGG 3 cut(s) 18, 248, 366
Bse3DI GCAATG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 238
BseMI GCAATG 1 cut(s) 228
BseNI ACTGG 3 cut(s) 18, 248, 366
BseXI GCAGC 3 cut(s) 138, 180, 439
BsgI GTGCAG 1 cut(s) 438
BsiSI CCGG 2 cut(s) 398, 463
BslFI GGGAC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 236
Bsp1407I TGTACA 1 cut(s) 220
Bsp143I GATC 3 cut(s) 76, 109, 136
BspACI CCGC 1 cut(s) 195
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 78
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 173
BspPI GGATC 2 cut(s) 71, 84
BsrDI GCAATG 1 cut(s) 228
BsrGI TGTACA 1 cut(s) 220
BsrI ACTGG 3 cut(s) 18, 248, 366
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 3 cut(s) 76, 109, 136
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 79
BstAPI GCANNNNNTGC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 220
BstC8I GCNNGC 3 cut(s) 6, 235, 392
BstKTI GATC 3 cut(s) 79, 112, 139
BstMBI GATC 3 cut(s) 76, 109, 136
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstNSI RCATGY 2 cut(s) 221, 237
BstSFI CTRYAG 2 cut(s) 166, 327
BstV1I GCAGC 3 cut(s) 138, 180, 439
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BveI ACCTGC 1 cut(s) 173
Cac8I GCNNGC 3 cut(s) 6, 235, 392
CciI TCATGA 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 367
Csp6I GTAC 2 cut(s) 160, 221
CviAII CATG 4 cut(s) 26, 107, 218, 234
CviJI RGCY 7 cut(s) 19, 209, 352, 394, 408, 452, 469
CviKI_1 RGCY 7 cut(s) 19, 209, 352, 394, 408, 452, 469
CviQI GTAC 2 cut(s) 160, 221
DpnI GATC 3 cut(s) 78, 111, 138
DpnII GATC 3 cut(s) 76, 109, 136
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 367
FaeI CATG 4 cut(s) 29, 110, 221, 237
FalI AAGNNNNNCTT 4 cut(s) 329, 361, 378, 410
FaqI GGGAC 1 cut(s) 236
FatI CATG 4 cut(s) 25, 106, 217, 233
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 152, 169, 453
Fsp4HI GCNGC 3 cut(s) 152, 169, 453
FspBI CTAG 1 cut(s) 458
GluI GCNGC 3 cut(s) 152, 169, 453
GsuI CTGGAG 2 cut(s) 35, 383
HapII CCGG 2 cut(s) 398, 463
Hin1II CATG 4 cut(s) 29, 110, 221, 237
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 2 cut(s) 267, 323
HpaII CCGG 2 cut(s) 398, 463
HphI GGTGA 2 cut(s) 55, 412
Hpy166II GTNNAC 2 cut(s) 162, 327
Hpy188I TCNGA 4 cut(s) 36, 81, 182, 266
Hpy188III TCNNGA 3 cut(s) 107, 346, 370
Hpy8I GTNNAC 2 cut(s) 162, 327
HpyAV CCTTC 2 cut(s) 64, 380
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 6 cut(s) 100, 168, 217, 233, 429, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 4 cut(s) 29, 110, 221, 237
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 3 cut(s) 76, 109, 136
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 8 cut(s) 59, 178, 261, 315, 347, 383, 411, 423
Lsp1109I GCAGC 3 cut(s) 138, 180, 439
LweI GCATC 2 cut(s) 69, 180
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 88, 317, 412
MalI GATC 3 cut(s) 78, 111, 138
MboI GATC 3 cut(s) 76, 109, 136
MboII GAAGA 1 cut(s) 96
MflI RGATCY 1 cut(s) 76
MluCI AATT 2 cut(s) 183, 212
MlyI GAGTC 1 cut(s) 332
MnlI CCTC 5 cut(s) 67, 233, 285, 342, 358
MseI TTAA 1 cut(s) 422
MspI CCGG 2 cut(s) 398, 463
MwoI GCNNNNNNNGC 1 cut(s) 234
NdeII GATC 3 cut(s) 76, 109, 136
NlaIII CATG 4 cut(s) 29, 110, 221, 237
NlaIV GGNNCC 1 cut(s) 78
NmeAIII GCCGAG 1 cut(s) 263
NspI RCATGY 2 cut(s) 221, 237
PaeI GCATGC 1 cut(s) 237
PagI TCATGA 1 cut(s) 106
PaqCI CACCTGC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 267
PkrI GCNGC 3 cut(s) 153, 170, 454
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PpuMI RGGWCCY 1 cut(s) 367
Psp5II RGGWCCY 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 367
PspPPI RGGWCCY 1 cut(s) 367
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 2 cut(s) 161, 222
RsaNI GTAC 2 cut(s) 160, 221
SaqAI TTAA 1 cut(s) 422
SatI GCNGC 3 cut(s) 152, 169, 453
Sau3AI GATC 3 cut(s) 76, 109, 136
Sau96I GGNCC 1 cut(s) 367
SchI GAGTC 1 cut(s) 332
SetI ASST 9 cut(s) 21, 48, 134, 167, 244, 277, 369, 396, 454
SfaNI GCATC 2 cut(s) 69, 180
SfcI CTRYAG 2 cut(s) 166, 327
SinI GGWCC 1 cut(s) 367
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
SphI GCATGC 1 cut(s) 237
Sse9I AATT 2 cut(s) 183, 212
SsiI CCGC 1 cut(s) 195
SspI AATATT 1 cut(s) 376
SspMI CTAG 1 cut(s) 458
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 135
TasI AATT 2 cut(s) 183, 212
TatI WGTACW 2 cut(s) 159, 220
TfiI GAWTC 1 cut(s) 267
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TseI GCWGC 3 cut(s) 151, 168, 452
TspDTI ATGAA 3 cut(s) 42, 95, 296
VpaK11BI GGWCC 1 cut(s) 367
XceI RCATGY 2 cut(s) 221, 237
XmiI GTMKAC 1 cut(s) 326
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.