Rh2CG484300

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0020656
Physical Location & Seq
Reverse (-)
65004991 .. 65005278
288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG484300.1

Sequence Viewer

Length: 288 bp
ATGCAAGTTCATGATCATACAACAGTAATCATTACGTTCGATCTTATTTATATGCTGCATAAGTACACCTGCAGCATCATCATCAGAATTAGCACCACCGCCGTTGGCAAAGCCAATTTGCATGTACAACGCATTGCATGCCGAGGTGTTCCAGTCTCATACTCATTCGGAATCGCACCTCATAGATATTGCTACTTTGACGAGTGGTTTCATATTGACTGTAACGAGTCTACAGGGAACAAGCCTTTCTTGAGGCTCACTCAACTGGAGGGTCCTGAATATTTCTAA

Protein Analysis

95

Amino Acids

11.04

Weight (kDa)

6.99

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 46 - 87 7.8e-06 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020656)

Species Orthologous Gene IDs
rosa_laevigata RLG00000020658
rosa_multiflora Rmu_ssc0000489.1_g000005
rosa_samantha Rh2AG498200 Rh2BG509400 Rh2CG484300 Rh2DG521100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 77
AccI GTMKAC 1 cut(s) 230
AciI CCGC 1 cut(s) 99
AfaI GTAC 2 cut(s) 65, 126
Alw26I GTCTC 1 cut(s) 160
ApeKI GCWGC 2 cut(s) 55, 72
AspS9I GGNCC 1 cut(s) 272
AvaII GGWCC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 42, 84
BceAI ACGGC 1 cut(s) 86
BclI TGATCA 1 cut(s) 13
BcoDI GTCTC 1 cut(s) 160
BfmI CTRYAG 2 cut(s) 70, 231
BfuAI ACCTGC 1 cut(s) 77
BisI GCNGC 2 cut(s) 56, 73
BlsI GCNGC 2 cut(s) 57, 74
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 273
BmsI GCATC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 244, 276
BpmI CTGGAG 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 152, 270
Bse3DI GCAATG 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 132
BseNI ACTGG 2 cut(s) 152, 270
BseXI GCAGC 2 cut(s) 42, 84
BsmAI GTCTC 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 2 cut(s) 13, 40
BspACI CCGC 1 cut(s) 99
BspHI TCATGA 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 74
BspMI ACCTGC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 132
BsrGI TGTACA 1 cut(s) 124
BsrI ACTGG 2 cut(s) 152, 270
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 2 cut(s) 13, 40
Bst4CI ACNGT 2 cut(s) 25, 221
BstAPI GCANNNNNTGC 1 cut(s) 138
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 139
BstKTI GATC 2 cut(s) 16, 43
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 2 cut(s) 13, 40
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstNSI RCATGY 2 cut(s) 125, 141
BstSFI CTRYAG 2 cut(s) 70, 231
BstV1I GCAGC 2 cut(s) 42, 84
BveI ACCTGC 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 139
CciI TCATGA 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 272
Csp6I GTAC 2 cut(s) 64, 125
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 3 cut(s) 11, 122, 138
CviJI RGCY 3 cut(s) 113, 244, 256
CviKI_1 RGCY 3 cut(s) 113, 244, 256
CviQI GTAC 2 cut(s) 64, 125
DpnI GATC 2 cut(s) 15, 42
DpnII GATC 2 cut(s) 13, 40
Eco47I GGWCC 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 272
FaeI CATG 3 cut(s) 14, 125, 141
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 3 cut(s) 10, 121, 137
FbaI TGATCA 1 cut(s) 13
FblI GTMKAC 1 cut(s) 230
Fnu4HI GCNGC 2 cut(s) 56, 73
Fsp4HI GCNGC 2 cut(s) 56, 73
GluI GCNGC 2 cut(s) 56, 73
GsuI CTGGAG 1 cut(s) 287
Hin1II CATG 3 cut(s) 14, 125, 141
HinfI GANTC 2 cut(s) 171, 227
Hpy166II GTNNAC 2 cut(s) 66, 231
Hpy188I TCNGA 2 cut(s) 86, 170
Hpy188III TCNNGA 3 cut(s) 11, 250, 275
Hpy8I GTNNAC 2 cut(s) 66, 231
HpyCH4III ACNGT 2 cut(s) 25, 221
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 5 cut(s) 4, 58, 72, 121, 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 3 cut(s) 14, 125, 141
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 2 cut(s) 13, 40
LpnPI CCDG 4 cut(s) 82, 165, 219, 251
Lsp1109I GCAGC 2 cut(s) 42, 84
LweI GCATC 1 cut(s) 84
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 1 cut(s) 221
MalI GATC 2 cut(s) 15, 42
MboI GATC 2 cut(s) 13, 40
MluCI AATT 2 cut(s) 87, 115
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 4 cut(s) 137, 189, 246, 262
MwoI GCNNNNNNNGC 1 cut(s) 138
NdeII GATC 2 cut(s) 13, 40
NlaIII CATG 3 cut(s) 14, 125, 141
NlaIV GGNNCC 1 cut(s) 273
NmeAIII GCCGAG 1 cut(s) 167
NspI RCATGY 2 cut(s) 125, 141
PaeI GCATGC 1 cut(s) 141
PagI TCATGA 1 cut(s) 10
PaqCI CACCTGC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 171
PkrI GCNGC 2 cut(s) 57, 74
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
PpuMI RGGWCCY 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 272
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 1 cut(s) 272
PspPPI RGGWCCY 1 cut(s) 272
PstI CTGCAG 1 cut(s) 74
RsaI GTAC 2 cut(s) 65, 126
RsaNI GTAC 2 cut(s) 64, 125
SatI GCNGC 2 cut(s) 56, 73
Sau3AI GATC 2 cut(s) 13, 40
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 236
SetI ASST 4 cut(s) 38, 71, 148, 181
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 2 cut(s) 70, 231
SinI GGWCC 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
SphI GCATGC 1 cut(s) 141
Sse9I AATT 2 cut(s) 87, 115
SsiI CCGC 1 cut(s) 99
SspI AATATT 1 cut(s) 281
TaaI ACNGT 2 cut(s) 25, 221
TaiI ACGT 1 cut(s) 38
TaqI TCGA 1 cut(s) 39
TasI AATT 2 cut(s) 87, 115
TatI WGTACW 2 cut(s) 63, 124
TfiI GAWTC 1 cut(s) 171
TseI GCWGC 2 cut(s) 55, 72
TspDTI ATGAA 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 272
XceI RCATGY 2 cut(s) 125, 141
XmiI GTMKAC 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.