RLG00000021617

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019022
Physical Location & Seq
Forward (+)
76755251 .. 76755980
730 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021617

Sequence Viewer

Length: 387 bp
ATGGCCCAGAGTCTTCACTTTACAGAAGAGGTTCGATCCACAAAATGTCATCTAGGTGATTTTAAGGTTAAGATAGAGTTTGACGACCTGAAGAATAAGGCCCTTCATACGTGCAGTTGCTTGAGTTCCCTTGTTAGTCAAATTGAGAGAACACTTAATTACCAGCAATATTTGGTTGCCAAAGAGTTGAAGAAGCAATCTTGGAGATACCACAGGAAATACAACACTTGGTTTCAACGACACAAAGAGCCAAAAGTTGCTACTGACGAATATAAACAGGGTACATATGTGTACTTTGATTTCCATATTGCCAATGATGATCTACAACATGGATGGTACGATTTAGAGTTTGTATTTGGGATTTCGGAATTCATTTGTGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

129

Amino Acids

15.34

Weight (kDa)

7.04

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOT2_3_5_C PF04153 53 - 102 1.2e-17 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019022)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0052311
rosa_laevigata RLG00000021617
rosa_roxburghii Rroxscaffold_2G00077060 Rroxscaffold_5G00364980 Rroxscaffold_5G00365010
rosa_samantha Rh1BG215100 Rh1DG241100 Rh5BG356500 Rh5DG371700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 30
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 110
AfaI GTAC 3 cut(s) 283, 293, 338
AgsI TTSAA 2 cut(s) 190, 236
AlwI GGATC 1 cut(s) 30
AoxI GGCC 2 cut(s) 3, 99
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 4, 100
AsuHPI GGTGA 1 cut(s) 68
BaeI ACNNNNGTAYC 2 cut(s) 273, 306
BbsI GAAGAC 1 cut(s) 5
BccI CCATC 1 cut(s) 327
BfaI CTAG 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 4, 100
BpiI GAAGAC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 142
BsaAI YACGTR 1 cut(s) 111
BseGI GGATG 1 cut(s) 338
BsgI GTGCAG 1 cut(s) 133
BshFI GGCC 2 cut(s) 5, 101
BsnI GGCC 2 cut(s) 5, 101
Bsp143I GATC 2 cut(s) 35, 319
BspANI GGCC 2 cut(s) 5, 101
BspPI GGATC 1 cut(s) 30
BssMI GATC 2 cut(s) 35, 319
Bst6I CTCTTC 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 111
BstF5I GGATG 1 cut(s) 338
BstKTI GATC 2 cut(s) 38, 322
BstMBI GATC 2 cut(s) 35, 319
BstV2I GAAGAC 1 cut(s) 5
BsuRI GGCC 2 cut(s) 5, 101
BtsCI GGATG 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 4, 100
Csp6I GTAC 3 cut(s) 282, 292, 337
CviAII CATG 1 cut(s) 329
CviJI RGCY 3 cut(s) 5, 101, 250
CviKI_1 RGCY 3 cut(s) 5, 101, 250
CviQI GTAC 3 cut(s) 282, 292, 337
DpnI GATC 2 cut(s) 37, 321
DpnII GATC 2 cut(s) 35, 319
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 368
FaeI CATG 1 cut(s) 332
FaiI YATR 6 cut(s) 108, 273, 286, 288, 306, 330
FatI CATG 1 cut(s) 328
FauNDI CATATG 1 cut(s) 286
FokI GGATG 1 cut(s) 345
FspBI CTAG 1 cut(s) 53
HaeIII GGCC 2 cut(s) 5, 101
Hin1II CATG 1 cut(s) 332
HinfI GANTC 1 cut(s) 10
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 1 cut(s) 113
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 110
Hsp92II CATG 1 cut(s) 332
Kzo9I GATC 2 cut(s) 35, 319
LpnPI CCDG 5 cut(s) 20, 101, 176, 199, 263
MaeI CTAG 1 cut(s) 53
MaeII ACGT 1 cut(s) 110
MalI GATC 2 cut(s) 37, 321
MboI GATC 2 cut(s) 35, 319
MboII GAAGA 4 cut(s) 5, 38, 103, 202
MluCI AATT 3 cut(s) 141, 157, 368
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 1 cut(s) 22
MroXI GAANNNNTTC 1 cut(s) 30
MseI TTAA 3 cut(s) 63, 69, 156
MslI CAYNNNNRTG 1 cut(s) 54
NdeI CATATG 1 cut(s) 286
NdeII GATC 2 cut(s) 35, 319
NlaIII CATG 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 30
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
Ppu21I YACGTR 1 cut(s) 111
PspPI GGNCC 2 cut(s) 4, 100
RsaI GTAC 3 cut(s) 283, 293, 338
RsaNI GTAC 3 cut(s) 282, 292, 337
RseI CAYNNNNRTG 1 cut(s) 54
SaqAI TTAA 3 cut(s) 63, 69, 156
Sau3AI GATC 2 cut(s) 35, 319
Sau96I GGNCC 2 cut(s) 4, 100
SchI GAGTC 1 cut(s) 19
SetI ASST 5 cut(s) 33, 58, 69, 90, 113
SmiMI CAYNNNNRTG 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 3 cut(s) 141, 157, 368
SspI AATATT 1 cut(s) 170
SspMI CTAG 1 cut(s) 53
TaiI ACGT 1 cut(s) 113
TaqI TCGA 1 cut(s) 34
TasI AATT 3 cut(s) 141, 157, 368
TatI WGTACW 1 cut(s) 291
Tru1I TTAA 3 cut(s) 63, 69, 156
Tru9I TTAA 3 cut(s) 63, 69, 156
TspDTI ATGAA 2 cut(s) 95, 361
XapI RAATTY 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 30
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.