RLG00000021663

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019832
Physical Location & Seq
Reverse (-)
77088771 .. 77089342
572 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021663

Sequence Viewer

Length: 465 bp
ATGCTATCGTTGTACGAAGAACACTTGGAGCTCCCAGGGAGGAATGAGAGTGATGAACACATTAAAAAAAGAGCAGCCTTCTGGTCAACAGTGAAGCCAGCTCATTTCGGGGTGAAGATAGGCACCAAGTCTCTGTTGGGTATTTTTCGAATCATGACAGTTGGGATCTTTATTGGGCTCTTGGGTCAGTTTTCCTTTGGGAGCGACAGGGGTCTTGCATCTCAGGGAAACACAAAACCTGATATGGAAATAGTAACAAGAGTTATGGGACCTGAAATTGGCTACTTAACAACCCCGATAATTCTGCTTGAGTGTGCACTTAGTGGTCTGAGCCAGCGCGACAATCTTCCAAAGGGAGGAGTTTTCCCAGCTGGGATTGTATTTGGCCCAACTGATCTCCAAGAAAGGCGACAAGTAAATGGAGTTACTTTTGATGTTATTTTGAAGAATGCTATTTCTCATTAG

Protein Analysis

155

Amino Acids

16.84

Weight (kDa)

7.92

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019832)

Species Orthologous Gene IDs
pyrus_communis pycom17g05430
rosa_chinensis RchiOBHm_Chr4g0415101
rosa_laevigata RLG00000021663 RLG00000021666
rosa_multiflora Rmu_sc0021992.1_g000008
rosa_samantha Rh2DG614100 Rh4CG202800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccII CGCG 1 cut(s) 339
AclWI GGATC 1 cut(s) 173
AdeI CACNNNGTG 1 cut(s) 323
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 2 cut(s) 278, 356
AgsI TTSAA 1 cut(s) 445
AjnI CCWGG 1 cut(s) 34
AluBI AGCT 3 cut(s) 31, 101, 371
AluI AGCT 3 cut(s) 31, 101, 371
Alw21I GWGCWC 2 cut(s) 33, 319
Alw26I GTCTC 1 cut(s) 135
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 1 cut(s) 173
AoxI GGCC 1 cut(s) 385
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 2 cut(s) 269, 386
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 2 cut(s) 33, 180
Bbv12I GWGCWC 2 cut(s) 33, 319
BbvI GCAGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciT130I CCWGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 135
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 2 cut(s) 269, 386
BmiI GGNNCC 2 cut(s) 124, 270
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 227
BoxI GACNNNNGTC 1 cut(s) 210
Bpu14I TTCGAA 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 329
BsaJI CCNNGG 2 cut(s) 34, 35
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 356
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 34, 35
BseLI CCNNNNNNNGG 2 cut(s) 278, 356
BseMII CTCAG 2 cut(s) 236, 320
BseRI GAGGAG 1 cut(s) 372
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 1 cut(s) 86
BseYI CCCAGC 2 cut(s) 367, 371
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 1 cut(s) 387
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 2 cut(s) 33, 319
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 278, 356
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 1 cut(s) 387
Bsp119I TTCGAA 1 cut(s) 148
Bsp1286I GDGCHC 3 cut(s) 33, 180, 319
Bsp143I GATC 2 cut(s) 165, 394
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 2 cut(s) 235, 321
BspFNI CGCG 1 cut(s) 339
BspHI TCATGA 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 124, 270
BspPI GGATC 1 cut(s) 173
BspT104I TTCGAA 1 cut(s) 148
BspT107I GGYRCC 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 34, 35
BssMI GATC 2 cut(s) 165, 394
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 91, 160
BstBI TTCGAA 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 99, 335
BstDEI CTNAG 3 cut(s) 222, 320, 329
BstFNI CGCG 1 cut(s) 339
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 2 cut(s) 168, 397
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 165, 394
BstNI CCWGG 1 cut(s) 36
BstPAI GACNNNNGTC 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 34
BstSLI GKGCMC 1 cut(s) 319
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuRI GGCC 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 2 cut(s) 99, 335
CciI TCATGA 1 cut(s) 153
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 2 cut(s) 269, 386
Csp6I GTAC 1 cut(s) 13
CviAII CATG 1 cut(s) 154
CviJI RGCY 9 cut(s) 31, 77, 97, 101, 178, 282, 333, 371, 387
CviKI_1 RGCY 9 cut(s) 31, 77, 97, 101, 178, 282, 333, 371, 387
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 3 cut(s) 222, 320, 329
DpnI GATC 2 cut(s) 167, 396
DpnII GATC 2 cut(s) 165, 394
DraIII CACNNNGTG 1 cut(s) 323
Ecl136II GAGCTC 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 33, 180
Eco47I GGWCC 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 34
EcoT38I GRGCYC 2 cut(s) 33, 180
FaeI CATG 1 cut(s) 157
FaiI YATR 3 cut(s) 155, 245, 266
FaqI GGGAC 1 cut(s) 282
FatI CATG 1 cut(s) 153
Fnu4HI GCNGC 1 cut(s) 75
FriOI GRGCYC 2 cut(s) 33, 180
Fsp4HI GCNGC 1 cut(s) 75
GlaI GCGC 1 cut(s) 338
GluI GCNGC 1 cut(s) 75
GsaI CCCAGC 2 cut(s) 371, 375
HaeIII GGCC 1 cut(s) 387
HhaI GCGC 1 cut(s) 339
Hin1II CATG 1 cut(s) 157
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 1 cut(s) 150
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 2 cut(s) 87, 317
Hpy188I TCNGA 1 cut(s) 330
Hpy188III TCNNGA 1 cut(s) 154
Hpy8I GTNNAC 2 cut(s) 87, 317
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 2 cut(s) 91, 160
HpyCH4V TGCA 2 cut(s) 218, 317
HpyF3I CTNAG 3 cut(s) 222, 320, 329
Hsp92II CATG 1 cut(s) 157
HspAI GCGC 1 cut(s) 337
Kzo9I GATC 2 cut(s) 165, 394
LmnI GCTCC 3 cut(s) 28, 36, 201
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 1 cut(s) 227
MaeIII GTNAC 2 cut(s) 253, 424
MalI GATC 2 cut(s) 167, 396
MboI GATC 2 cut(s) 165, 394
MboII GAAGA 4 cut(s) 29, 127, 338, 457
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 3 cut(s) 33, 180, 319
MluCI AATT 2 cut(s) 276, 300
MnlI CCTC 2 cut(s) 33, 350
MseI TTAA 2 cut(s) 63, 287
MspA1I CMGCKG 1 cut(s) 371
MspR9I CCNGG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 1 cut(s) 339
NdeII GATC 2 cut(s) 165, 394
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 2 cut(s) 124, 270
NspV TTCGAA 1 cut(s) 148
PagI TCATGA 1 cut(s) 153
PasI CCCWGGG 1 cut(s) 35
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 150
PkrI GCNGC 1 cut(s) 76
PpuMI RGGWCCY 1 cut(s) 269
PshAI GACNNNNGTC 1 cut(s) 210
Psp124BI GAGCTC 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 34
PspFI CCCAGC 2 cut(s) 367, 371
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 124, 270
PspPI GGNCC 2 cut(s) 269, 386
PspPPI RGGWCCY 1 cut(s) 269
PsuI RGATCY 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 63, 287
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 2 cut(s) 165, 394
Sau96I GGNCC 2 cut(s) 269, 386
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 3 cut(s) 33, 180, 319
SetI ASST 5 cut(s) 33, 103, 241, 274, 373
SfaNI GCATC 1 cut(s) 227
SfuI TTCGAA 1 cut(s) 148
SinI GGWCC 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 2 cut(s) 276, 300
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 34
TaaI ACNGT 2 cut(s) 91, 160
TaqI TCGA 1 cut(s) 148
TasI AATT 2 cut(s) 276, 300
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 2 cut(s) 63, 287
Tru9I TTAA 2 cut(s) 63, 287
TscAI CASTG 1 cut(s) 96
TseI GCWGC 1 cut(s) 74
TspDTI ATGAA 1 cut(s) 69
TspRI CASTG 1 cut(s) 96
VneI GTGCAC 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 269
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.