RLG00000023164

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0022960
Physical Location & Seq
Reverse (-)
19282872 .. 19286015
3144 bp
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UTR
Exon/CDS
Intron
RLM00000023164

Sequence Viewer

Length: 564 bp
ATGCGGACCCTCCATGACCAGCTTGAGGCTCCCAGATCTCCTGATCAACAACTCCGGTTGAGACACAAGTTAGCAGTTGCTGCTACCGTCGTGGTGGCTGTGCTGCTTCTTCTGACGAAGCAGACCCGACATGGGGGAGCCGGAGCAAACGGGGCTGGTGGCGCAGGAGCGAGAGGGGATGAGGCTGCGAACCACTTCGTCTTTAAGAGCCTTACTTCTTCTCCATTTCTAGAGAGTCGGTTTCAGACTTTAGCCTTATTCTTTCCTCATGACCCGGTTTCTTGGTTGAAAGGGGAAATGGATTTCAATGGGAGTCTTGAGCTTGTAGATGGTAAGCTGAATTCAGTGTGGTCAACCAATGTTACTACACAGGTTCCATCATCTAATACTAGTTCATTTGCTGCATTTCTTTCAGATGAAGGAAACTTTGTTGTTATTAGAGCTGGTGAATCAATATGGCAGAGCTTTTATTATGTTGGTGAAACTATGCTACCGACCCAGCAGGTGGGATACGATAGTAAATCTAAAACGGGCCATTTCTTGACTGCATGCCTGGAAAAGTGA

Protein Analysis

188

Amino Acids

20.33

Weight (kDa)

6.58

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 100 - 183 8.6e-13 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0022960)

Species Orthologous Gene IDs
rosa_laevigata RLG00000023164
rosa_samantha Rh3DG315100 Rh6BG439000 Rh6CG476200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 493
Acc36I ACCTGC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 505
AciI CCGC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 340
AfiI CCNNNNNNNGG 4 cut(s) 25, 132, 133, 505
AgsI TTSAA 2 cut(s) 289, 307
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 1 cut(s) 552
AluBI AGCT 5 cut(s) 22, 322, 337, 443, 465
AluI AGCT 5 cut(s) 22, 322, 337, 443, 465
Alw26I GTCTC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 1 cut(s) 532
ApeKI GCWGC 4 cut(s) 80, 103, 185, 401
ApoI RAATTY 1 cut(s) 340
Asp700I GAANNNNTTC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 6, 532
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 458, 491
AvaII GGWCC 1 cut(s) 6
BaeI ACNNNNGTAYC 2 cut(s) 502, 535
BbvI GCAGC 4 cut(s) 67, 90, 172, 388
BccI CCATC 2 cut(s) 323, 385
BciT130I CCWGG 1 cut(s) 554
BciVI GTATCC 1 cut(s) 503
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 55
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 2 cut(s) 230, 390
BfuAI ACCTGC 1 cut(s) 493
BfuI GTATCC 1 cut(s) 503
BglII AGATCT 1 cut(s) 35
BisI GCNGC 4 cut(s) 81, 104, 186, 402
BlsI GCNGC 4 cut(s) 82, 105, 187, 403
Bme1390I CCNGG 2 cut(s) 275, 554
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 2 cut(s) 6, 532
BmiI GGNNCC 4 cut(s) 8, 30, 139, 375
BmrFI CCNGG 2 cut(s) 275, 554
BpuEI CTTGAG 2 cut(s) 44, 338
BpuMI CCSGG 1 cut(s) 275
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 132, 133, 505
BseBI CCWGG 1 cut(s) 554
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 4 cut(s) 25, 132, 133, 505
BseXI GCAGC 4 cut(s) 67, 90, 172, 388
BseYI CCCAGC 1 cut(s) 498
BshFI GGCC 1 cut(s) 534
BsiSI CCGG 3 cut(s) 55, 141, 275
BslI CCNNNNNNNGG 4 cut(s) 25, 132, 133, 505
BsmAI GTCTC 1 cut(s) 55
BsnI GGCC 1 cut(s) 534
Bsp143I GATC 2 cut(s) 35, 43
BspACI CCGC 1 cut(s) 4
BspANI GGCC 1 cut(s) 534
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 4 cut(s) 8, 30, 139, 375
BspMI ACCTGC 1 cut(s) 493
BssMI GATC 2 cut(s) 35, 43
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 550
BstF5I GGATG 1 cut(s) 184
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 2 cut(s) 38, 46
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 2 cut(s) 35, 43
BstMWI GCNNNNNNNGC 3 cut(s) 80, 152, 161
BstNI CCWGG 1 cut(s) 554
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 2 cut(s) 273, 552
BstV1I GCAGC 4 cut(s) 67, 90, 172, 388
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuI GTATCC 1 cut(s) 503
BsuRI GGCC 1 cut(s) 534
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 351
BveI ACCTGC 1 cut(s) 493
Cac8I GCNNGC 1 cut(s) 550
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 6, 532
CviAII CATG 4 cut(s) 14, 131, 269, 549
DpnI GATC 2 cut(s) 37, 45
DpnII GATC 2 cut(s) 35, 43
Eco47I GGWCC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 552
FaeI CATG 4 cut(s) 17, 134, 272, 552
FaiI YATR 7 cut(s) 15, 132, 270, 457, 474, 488, 550
FatI CATG 4 cut(s) 13, 130, 268, 548
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 81, 104, 186, 402
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 4 cut(s) 81, 104, 186, 402
FspBI CTAG 2 cut(s) 230, 390
GlaI GCGC 1 cut(s) 163
GluI GCNGC 4 cut(s) 81, 104, 186, 402
GsaI CCCAGC 1 cut(s) 502
HaeIII GGCC 1 cut(s) 534
HapII CCGG 3 cut(s) 55, 141, 275
HhaI GCGC 1 cut(s) 164
Hin1II CATG 4 cut(s) 17, 134, 272, 552
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 3 cut(s) 235, 313, 449
HpaII CCGG 3 cut(s) 55, 141, 275
HphI GGTGA 2 cut(s) 458, 491
Hpy166II GTNNAC 1 cut(s) 354
Hpy188I TCNGA 3 cut(s) 114, 246, 415
Hpy188III TCNNGA 5 cut(s) 41, 230, 269, 317, 541
Hpy8I GTNNAC 1 cut(s) 354
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4V TGCA 2 cut(s) 404, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 152, 161
Hsp92II CATG 4 cut(s) 17, 134, 272, 552
HspAI GCGC 1 cut(s) 162
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 2 cut(s) 35, 43
LmnI GCTCC 4 cut(s) 34, 137, 143, 167
Lsp1109I GCAGC 4 cut(s) 67, 90, 172, 388
MaeI CTAG 2 cut(s) 230, 390
MaeIII GTNAC 1 cut(s) 361
MalI GATC 2 cut(s) 37, 45
MboI GATC 2 cut(s) 35, 43
MboII GAAGA 2 cut(s) 101, 210
MflI RGATCY 1 cut(s) 35
MluCI AATT 1 cut(s) 340
MlyI GAGTC 2 cut(s) 244, 322
MnlI CCTC 5 cut(s) 19, 20, 167, 175, 276
MroXI GAANNNNTTC 1 cut(s) 194
MseI TTAA 1 cut(s) 204
MspI CCGG 3 cut(s) 55, 141, 275
MspR9I CCNGG 2 cut(s) 275, 554
MvaI CCWGG 1 cut(s) 554
MwoI GCNNNNNNNGC 3 cut(s) 80, 152, 161
NciI CCSGG 1 cut(s) 275
NdeII GATC 2 cut(s) 35, 43
NlaIII CATG 4 cut(s) 17, 134, 272, 552
NlaIV GGNNCC 4 cut(s) 8, 30, 139, 375
NspI RCATGY 1 cut(s) 552
PaeI GCATGC 1 cut(s) 552
PagI TCATGA 1 cut(s) 268
PaqCI CACCTGC 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 4 cut(s) 82, 105, 187, 403
PleI GAGTC 2 cut(s) 243, 321
PpsI GAGTC 2 cut(s) 243, 321
Psp6I CCWGG 1 cut(s) 552
PspFI CCCAGC 1 cut(s) 498
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 4 cut(s) 8, 30, 139, 375
PspPI GGNCC 2 cut(s) 6, 532
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 35
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 4 cut(s) 81, 104, 186, 402
Sau3AI GATC 2 cut(s) 35, 43
Sau96I GGNCC 2 cut(s) 6, 532
SchI GAGTC 2 cut(s) 244, 322
ScrFI CCNGG 2 cut(s) 275, 554
SetI ASST 7 cut(s) 24, 324, 339, 375, 445, 467, 507
SinI GGWCC 1 cut(s) 6
SmlI CTYRAG 2 cut(s) 23, 317
SmoI CTYRAG 2 cut(s) 23, 317
SpeI ACTAGT 1 cut(s) 389
SphI GCATGC 1 cut(s) 552
Sse9I AATT 1 cut(s) 340
SsiI CCGC 1 cut(s) 4
SspMI CTAG 2 cut(s) 230, 390
StyD4I CCNGG 2 cut(s) 273, 552
TaaI ACNGT 1 cut(s) 88
TasI AATT 1 cut(s) 340
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 1 cut(s) 351
TseI GCWGC 4 cut(s) 80, 103, 185, 401
TspDTI ATGAA 2 cut(s) 384, 432
TspRI CASTG 1 cut(s) 351
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 6
XapI RAATTY 1 cut(s) 340
XbaI TCTAGA 1 cut(s) 229
XceI RCATGY 1 cut(s) 552
XmnI GAANNNNTTC 1 cut(s) 194
XspI CTAG 2 cut(s) 230, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.