Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0014295

Physical Location & Seq

Genomic Coordinates Forward (+)

24728168 .. 24731740

Gene Structure 3573 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023508

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGACATTGAACTCTTTGTTTCGATACTATTCGCACTCTGGTGGAATTGTAATGCTAGTGTTGTTAAGTTTTTGGTTTATATTTTGTGGGCAGGGTGCTTGGGAATCAATTGTGCGTGCTTTCGAGAAGGATTATATCTATCTTGGCGAAGCTGCGCAGATAATGGTTCAAAATGTGAACTATGAGATTCCGTATCAGAAAAAGCAGGTGCAGAAGATTCAGCAACAACTAGCTGAACTAGAGCACAAGGAAGCTGATATTAAGAGAACTGCAGCTCTATCGGCAGCCAAGTATGTGGAGGCTTGTCAAGAGCTTGGATTGAAGGGAAATAATGTAAGGGCGGAACTTTTAGAGACTGCAAAATCACTTCCTACCACATTCAGCAGGATATTGGAAGTGATAAATGGTGATTCTGTGTCGAGGGCTCTGGAGTATTATTCTAACTTTGTGAGAGATGCTCACACTGAAAAGGATAAAAATTTGGTTGTGTTAACAAACTTGAAAGATTTACGTGAGCATCCCCCATCATTGGATGTTTCTGTAGACTTGGAGGTTGTTGATTCTGGGAATGTTCAATCAAGCCATAATGAGTCAAACAATGTGACAGTGGATGCAGATGCTAATGCCAACAGTATAGACTGGGATTTTTCTGTGGACAGTGCTCAGATTGATTGGGATATTGGTACTGTGGAAGAAACAGAAGATGCTGGTAATGGTTTGGGCCCTTACGAAATGGTTAGTGCCAGCGAGATCTTGCAGAGTTCTCCAAATGAAGCAGCAAAATCCGATCAGGTCCCAGAGGTTTCTGTATCTGAGATATCTTGGGATATTAGTGTTGAAACTCCTCAAGTTGATGCAATTGATGATGTCAATTTGCTGAATGTTGTTGTTGACAATCAAACATCTGTTCTTCATACTTCAACTCCAACAACTGAAATCAAGGAAGGTAGGAGCCAACTTTTGGAAACAGAGTATAGGAACAAGATTCTGGATGATCTGAATGAGATCAAAGCTTTTCTAAATCAGCGGTTGGTTGAGTTGAAGAATGAAGAAACTTTGTCATTGCAACATCAAGTCCAGGCAGTTGCTCCCTTTGTGCTGCAGCAATATGCTCATGATGCCATTGAGACAATGCTATCTGATGTTTGCTTGGCCATTTCTTTGCTGACAAATAGGAAAACGCGGGACTTGATCATGATTCTCAACTCCAAAAGATTTCTAGACAGACTAGTGAGTTCATTGGAAGAAAAGAAGCATCACGAAGTGAAGTTGAAAGAGGGTTTGAAGGGCTTAGCTACCAAACGCATGGAGCTGCACAACTCGTTATCTTCTTCCTGGCCAAAGCAGGAAGCAGCTCTTGCAAAAACACGCGAGCTTAAGAAGCTGTGTGAGAGCACCCTTTCCTCCATGTTTGATGGTAGACCTGTTCATATAATTGGAGAGATCAATGCCCTTTTGAATAGTGGTGTTGCTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000079 GO:0001932 GO:0005575 GO:0005623 GO:0007346 GO:0008150 GO:0012505 GO:0019220 GO:0019222 GO:0031323 GO:0031399 GO:0032268 GO:0042325 GO:0043549 GO:0044464 GO:0045859 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051174 GO:0051246 GO:0051338 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071900 GO:0080090 GO:1904029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF773

Protein Analysis

493

Amino Acids

54.99

Weight (kDa)

4.88

Isoelectric Point (pI)

34.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDK5RAP3	PF05600	32 - 480	5.8e-132	CDK5 regulatory subunit-associated protein 3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014295)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06830
fragaria_vesca	FvH4_6g22670 FvH4_6g22670
malus_domestica	MD14G1060600.v1.1
prunus_persica	Prupe.7G073900_v2.0.a1 Prupe.7G073900_v2.0.a1
pyrus_communis	pycom14g04910
rosa_chinensis	RchiOBHm_Chr3g0480241
rosa_laevigata	RLG00000023508
rosa_multiflora	Rmu_sc0006148.1_g000001
rosa_roxburghii	Rroxscaffold_6G00401680
rosa_rugosa	Rorug03G0181400
rosa_samantha	Rh3AG233100 Rh3BG265700 Rh3CG263000 Rh3DG258900
rosa_wichuraiana	Rw3G020940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	196
Acc16I	TGCGCA	1 cut(s)	156
Acc36I	ACCTGC	1 cut(s)	196
AccB7I	CCANNNNNTGG	1 cut(s)	961
AccI	GTMKAC	2 cut(s)	543, 1420
AccII	CGCG	2 cut(s)	1183, 1371
AciI	CCGC	3 cut(s)	341, 1027, 1183
AcoI	YGGCCR	2 cut(s)	1152, 1337
AcsI	RAATTY	1 cut(s)	478
AdeI	CACNNNGTG	1 cut(s)	1264
AfaI	GTAC	1 cut(s)	685
AfiI	CCNNNNNNNGG	2 cut(s)	529, 961
AflII	CTTAAG	1 cut(s)	1376
AhlI	ACTAGT	1 cut(s)	1228
AjnI	CCWGG	2 cut(s)	1077, 1334
AleI	CACNNNNGTG	1 cut(s)	39
Alw21I	GWGCWC	3 cut(s)	246, 664, 1397
Alw26I	GTCTC	2 cut(s)	347, 1121
AoxI	GGCC	3 cut(s)	721, 1152, 1337
ApaI	GGGCCC	1 cut(s)	725
ApeKI	GCWGC	9 cut(s)	152, 272, 284, 776, 1099, 1102, 1312, 1352, 1472
ApoI	RAATTY	1 cut(s)	478
AspLEI	GCGC	1 cut(s)	157
AspS9I	GGNCC	3 cut(s)	721, 722, 793
AsuHPI	GGTGA	1 cut(s)	419
AvaII	GGWCC	1 cut(s)	793
BaeGI	GKGCMC	1 cut(s)	725
BalI	TGGCCA	2 cut(s)	1154, 1339
BanII	GRGCYC	2 cut(s)	427, 725
Bbv12I	GWGCWC	3 cut(s)	246, 664, 1397
BbvI	GCAGC	9 cut(s)	139, 284, 296, 788, 1086, 1114, 1299, 1364, 1459
BccI	CCATC	2 cut(s)	532, 1409
BcgI	CGANNNNNNTGC	2 cut(s)	261, 295
BciT130I	CCWGG	2 cut(s)	1079, 1336
BclI	TGATCA	1 cut(s)	1191
BcoDI	GTCTC	2 cut(s)	347, 1121
BcuI	ACTAGT	1 cut(s)	1228
BfaI	CTAG	5 cut(s)	56, 230, 239, 1220, 1229
BfmI	CTRYAG	3 cut(s)	270, 540, 1100
BfrI	CTTAAG	1 cut(s)	1376
BfuAI	ACCTGC	1 cut(s)	196
BglII	AGATCT	1 cut(s)	750
BisI	GCNGC	9 cut(s)	153, 273, 285, 777, 1100, 1103, 1313, 1353, 1473
BlpI	GCTNAGC	1 cut(s)	1291
BlsI	GCNGC	9 cut(s)	154, 274, 286, 778, 1101, 1104, 1314, 1354, 1474
Bme1390I	CCNGG	2 cut(s)	1079, 1336
Bme18I	GGWCC	1 cut(s)	793
BmgT120I	GGNCC	3 cut(s)	721, 722, 793
BmiI	GGNNCC	3 cut(s)	723, 795, 953
BmrFI	CCNGG	2 cut(s)	1079, 1336
BmrI	ACTGGG	1 cut(s)	649
BmsI	GCATC	8 cut(s)	445, 526, 601, 607, 694, 844, 1108, 1264
BmuI	ACTGGG	1 cut(s)	649
BplI	GAGNNNNNCTC	2 cut(s)	442, 474
BpmI	CTGGAG	1 cut(s)	449
Bpu1102I	GCTNAGC	1 cut(s)	1291
BpuEI	CTTGAG	1 cut(s)	831
BsaAI	YACGTR	1 cut(s)	512
BsaXI	ACNNNNNCTCC	2 cut(s)	907, 937
Bsc4I	CCNNNNNNNGG	2 cut(s)	529, 961
Bse1I	ACTGG	1 cut(s)	644
Bse3DI	GCAATG	1 cut(s)	1061
BseBI	CCWGG	2 cut(s)	1079, 1336
BseGI	GGATG	4 cut(s)	517, 538, 616, 997
BseLI	CCNNNNNNNGG	2 cut(s)	529, 961
BseMI	GCAATG	1 cut(s)	1061
BseMII	CTCAG	2 cut(s)	677, 804
BseNI	ACTGG	1 cut(s)	644
BseRI	GAGGAG	1 cut(s)	834
BseSI	GKGCMC	1 cut(s)	725
BseXI	GCAGC	9 cut(s)	139, 284, 296, 788, 1086, 1114, 1299, 1364, 1459
BsgI	GTGCAG	2 cut(s)	230, 1298
Bsh1236I	CGCG	2 cut(s)	1183, 1371
BshFI	GGCC	3 cut(s)	723, 1154, 1339
BsiHKAI	GWGCWC	3 cut(s)	246, 664, 1397
BslFI	GGGAC	2 cut(s)	779, 1199
BslI	CCNNNNNNNGG	2 cut(s)	529, 961
BsmAI	GTCTC	2 cut(s)	347, 1121
BsmFI	GGGAC	2 cut(s)	779, 1199
BsnI	GGCC	3 cut(s)	723, 1154, 1339
Bsp120I	GGGCCC	1 cut(s)	721
Bsp1286I	GDGCHC	5 cut(s)	246, 427, 664, 725, 1397
Bsp143I	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
Bsp1720I	GCTNAGC	1 cut(s)	1291
BspACI	CCGC	3 cut(s)	341, 1027, 1183
BspANI	GGCC	3 cut(s)	723, 1154, 1339
BspCNI	CTCAG	2 cut(s)	676, 805
BspFNI	CGCG	2 cut(s)	1183, 1371
BspHI	TCATGA	2 cut(s)	1114, 1194
BspLI	GGNNCC	3 cut(s)	723, 795, 953
BspMAI	CTGCAG	2 cut(s)	274, 1104
BspMI	ACCTGC	1 cut(s)	196
BspTI	CTTAAG	1 cut(s)	1376
BsrDI	GCAATG	1 cut(s)	1061
BsrI	ACTGG	1 cut(s)	644
BssMI	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
Bst2UI	CCWGG	2 cut(s)	1079, 1336
Bst4CI	ACNGT	4 cut(s)	607, 632, 659, 688
BstAFI	CTTAAG	1 cut(s)	1376
BstAPI	GCANNNNNTGC	1 cut(s)	1358
BstBAI	YACGTR	1 cut(s)	512
BstC8I	GCNNGC	3 cut(s)	117, 745, 1373
BstDEI	CTNAG	3 cut(s)	663, 813, 1291
BstF5I	GGATG	4 cut(s)	517, 538, 616, 997
BstFNI	CGCG	2 cut(s)	1183, 1371
BstHHI	GCGC	1 cut(s)	157
BstKTI	GATC	6 cut(s)	753, 790, 997, 1008, 1194, 1446
BstMAI	GTCTC	2 cut(s)	347, 1121
BstMBI	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
BstMWI	GCNNNNNNNGC	4 cut(s)	281, 1118, 1358, 1381
BstNI	CCWGG	2 cut(s)	1079, 1336
BstSCI	CCNGG	2 cut(s)	1077, 1334
BstSFI	CTRYAG	3 cut(s)	270, 540, 1100
BstSLI	GKGCMC	1 cut(s)	725
BstUI	CGCG	2 cut(s)	1183, 1371
BstV1I	GCAGC	9 cut(s)	139, 284, 296, 788, 1086, 1114, 1299, 1364, 1459
BstX2I	RGATCY	1 cut(s)	750
BstXI	CCANNNNNNTGG	2 cut(s)	295, 1306
BstYI	RGATCY	1 cut(s)	750
BsuRI	GGCC	3 cut(s)	723, 1154, 1339
BtsCI	GGATG	4 cut(s)	517, 538, 616, 997
BtsIMutI	CAGTG	3 cut(s)	462, 612, 664
BveI	ACCTGC	1 cut(s)	196
Cac8I	GCNNGC	3 cut(s)	117, 745, 1373
CciI	TCATGA	2 cut(s)	1114, 1194
CfoI	GCGC	1 cut(s)	157
Cfr13I	GGNCC	3 cut(s)	721, 722, 793
Csp6I	GTAC	1 cut(s)	684
CviAII	CATG	4 cut(s)	1115, 1195, 1306, 1408
CviQI	GTAC	1 cut(s)	684
DdeI	CTNAG	3 cut(s)	663, 813, 1291
DpnI	GATC	6 cut(s)	752, 789, 996, 1007, 1193, 1445
DpnII	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
DraIII	CACNNNGTG	1 cut(s)	1264
EaeI	YGGCCR	2 cut(s)	1152, 1337
EciI	GGCGGA	1 cut(s)	356
Eco24I	GRGCYC	2 cut(s)	427, 725
Eco32I	GATATC	1 cut(s)	819
Eco47I	GGWCC	1 cut(s)	793
EcoO109I	RGGNCCY	2 cut(s)	722, 793
EcoRII	CCWGG	2 cut(s)	1077, 1334
EcoRV	GATATC	1 cut(s)	819
EcoT38I	GRGCYC	2 cut(s)	427, 725
FaeI	CATG	4 cut(s)	1118, 1198, 1309, 1411
FalI	AAGNNNNNCTT	2 cut(s)	1341, 1373
FaqI	GGGAC	2 cut(s)	779, 1199
FatI	CATG	4 cut(s)	1114, 1194, 1305, 1407
FauI	CCCGC	1 cut(s)	1176
FbaI	TGATCA	1 cut(s)	1191
FblI	GTMKAC	2 cut(s)	543, 1420
Fnu4HI	GCNGC	9 cut(s)	153, 273, 285, 777, 1100, 1103, 1313, 1353, 1473
FokI	GGATG	4 cut(s)	504, 545, 623, 1004
FriOI	GRGCYC	2 cut(s)	427, 725
Fsp4HI	GCNGC	9 cut(s)	153, 273, 285, 777, 1100, 1103, 1313, 1353, 1473
FspBI	CTAG	5 cut(s)	56, 230, 239, 1220, 1229
FspI	TGCGCA	1 cut(s)	156
GlaI	GCGC	1 cut(s)	156
GluI	GCNGC	9 cut(s)	153, 273, 285, 777, 1100, 1103, 1313, 1353, 1473
GsuI	CTGGAG	1 cut(s)	449
HaeIII	GGCC	3 cut(s)	723, 1154, 1339
HhaI	GCGC	1 cut(s)	157
Hin1II	CATG	4 cut(s)	1118, 1198, 1309, 1411
Hin6I	GCGC	1 cut(s)	155
HinP1I	GCGC	1 cut(s)	155
HincII	GTYRAC	2 cut(s)	492, 892
HindII	GTYRAC	2 cut(s)	492, 892
HindIII	AAGCTT	1 cut(s)	1011
HinfI	GANTC	8 cut(s)	104, 187, 217, 410, 560, 590, 985, 1198
HpaI	GTTAAC	1 cut(s)	492
HphI	GGTGA	1 cut(s)	419
Hpy166II	GTNNAC	6 cut(s)	178, 492, 544, 655, 892, 1421
Hpy188I	TCNGA	6 cut(s)	198, 666, 787, 814, 999, 1141
Hpy188III	TCNNGA	8 cut(s)	124, 308, 428, 989, 1115, 1195, 1220, 1259
Hpy8I	GTNNAC	6 cut(s)	178, 492, 544, 655, 892, 1421
HpyAV	CCTTC	4 cut(s)	121, 316, 938, 1279
HpyCH4III	ACNGT	4 cut(s)	607, 632, 659, 688
HpyCH4IV	ACGT	1 cut(s)	511
HpyF10VI	GCNNNNNNNGC	4 cut(s)	281, 1118, 1358, 1381
HpyF3I	CTNAG	3 cut(s)	663, 813, 1291
HpySE526I	ACGT	1 cut(s)	511
Hsp92II	CATG	4 cut(s)	1118, 1198, 1309, 1411
HspAI	GCGC	1 cut(s)	155
Ksp22I	TGATCA	1 cut(s)	1191
KspAI	GTTAAC	1 cut(s)	492
Kzo9I	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
LmnI	GCTCC	3 cut(s)	951, 1093, 1309
Lsp1109I	GCAGC	9 cut(s)	139, 284, 296, 788, 1086, 1114, 1299, 1364, 1459
LweI	GCATC	8 cut(s)	445, 526, 601, 607, 694, 844, 1108, 1264
MaeI	CTAG	5 cut(s)	56, 230, 239, 1220, 1229
MaeII	ACGT	1 cut(s)	511
MaeIII	GTNAC	1 cut(s)	601
MalI	GATC	6 cut(s)	752, 789, 996, 1007, 1193, 1445
MboI	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
MboII	GAAGA	9 cut(s)	226, 704, 713, 902, 1054, 1061, 1256, 1320, 1323
MfeI	CAATTG	2 cut(s)	108, 858
MflI	RGATCY	1 cut(s)	750
MhlI	GDGCHC	5 cut(s)	246, 427, 664, 725, 1397
MlsI	TGGCCA	2 cut(s)	1154, 1339
MluCI	AATT	6 cut(s)	45, 108, 478, 858, 871, 1434
MluNI	TGGCCA	2 cut(s)	1154, 1339
MlyI	GAGTC	1 cut(s)	599
MmeI	TCCRAC	1 cut(s)	950
MnlI	CCTC	7 cut(s)	292, 414, 544, 793, 855, 1270, 1414
Mox20I	TGGCCA	2 cut(s)	1154, 1339
MscI	TGGCCA	2 cut(s)	1154, 1339
MseI	TTAA	4 cut(s)	65, 261, 491, 1377
MslI	CAYNNNNRTG	1 cut(s)	39
Msp20I	TGGCCA	2 cut(s)	1154, 1339
MspA1I	CMGCKG	1 cut(s)	1027
MspCI	CTTAAG	1 cut(s)	1376
MspR9I	CCNGG	2 cut(s)	1079, 1336
MunI	CAATTG	2 cut(s)	108, 858
MvaI	CCWGG	2 cut(s)	1079, 1336
MvnI	CGCG	2 cut(s)	1183, 1371
MwoI	GCNNNNNNNGC	4 cut(s)	281, 1118, 1358, 1381
NdeII	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
NlaIII	CATG	4 cut(s)	1118, 1198, 1309, 1411
NlaIV	GGNNCC	3 cut(s)	723, 795, 953
NmuCI	GTSAC	1 cut(s)	601
NsbI	TGCGCA	1 cut(s)	156
OliI	CACNNNNGTG	1 cut(s)	39
PagI	TCATGA	2 cut(s)	1114, 1194
PaqCI	CACCTGC	1 cut(s)	196
PfeI	GAWTC	7 cut(s)	104, 187, 217, 410, 560, 985, 1198
PflMI	CCANNNNNTGG	1 cut(s)	961
PkrI	GCNGC	9 cut(s)	154, 274, 286, 778, 1101, 1104, 1314, 1354, 1474
PleI	GAGTC	1 cut(s)	598
PpsI	GAGTC	1 cut(s)	598
Ppu21I	YACGTR	1 cut(s)	512
PpuMI	RGGWCCY	1 cut(s)	793
Psp5II	RGGWCCY	1 cut(s)	793
Psp6I	CCWGG	2 cut(s)	1077, 1334
PspGI	CCWGG	2 cut(s)	1077, 1334
PspN4I	GGNNCC	3 cut(s)	723, 795, 953
PspOMI	GGGCCC	1 cut(s)	721
PspPI	GGNCC	3 cut(s)	721, 722, 793
PspPPI	RGGWCCY	1 cut(s)	793
PstI	CTGCAG	2 cut(s)	274, 1104
PsuI	RGATCY	1 cut(s)	750
RsaI	GTAC	1 cut(s)	685
RsaNI	GTAC	1 cut(s)	684
RseI	CAYNNNNRTG	1 cut(s)	39
SaqAI	TTAA	4 cut(s)	65, 261, 491, 1377
SatI	GCNGC	9 cut(s)	153, 273, 285, 777, 1100, 1103, 1313, 1353, 1473
Sau3AI	GATC	6 cut(s)	750, 787, 994, 1005, 1191, 1443
Sau96I	GGNCC	3 cut(s)	721, 722, 793
SchI	GAGTC	1 cut(s)	599
ScrFI	CCNGG	2 cut(s)	1079, 1336
SduI	GDGCHC	5 cut(s)	246, 427, 664, 725, 1397
SfaNI	GCATC	8 cut(s)	445, 526, 601, 607, 694, 844, 1108, 1264
SfcI	CTRYAG	3 cut(s)	270, 540, 1100
SinI	GGWCC	1 cut(s)	793
SmiMI	CAYNNNNRTG	1 cut(s)	39
SmlI	CTYRAG	2 cut(s)	846, 1376
SmoI	CTYRAG	2 cut(s)	846, 1376
SpeI	ACTAGT	1 cut(s)	1228
Sse9I	AATT	6 cut(s)	45, 108, 478, 858, 871, 1434
SsiI	CCGC	3 cut(s)	341, 1027, 1183
SspMI	CTAG	5 cut(s)	56, 230, 239, 1220, 1229
StyD4I	CCNGG	2 cut(s)	1077, 1334
TaaI	ACNGT	4 cut(s)	607, 632, 659, 688
TaiI	ACGT	1 cut(s)	514
TaqI	TCGA	3 cut(s)	22, 123, 419
TasI	AATT	6 cut(s)	45, 108, 478, 858, 871, 1434
TfiI	GAWTC	7 cut(s)	104, 187, 217, 410, 560, 985, 1198
Tru1I	TTAA	4 cut(s)	65, 261, 491, 1377
Tru9I	TTAA	4 cut(s)	65, 261, 491, 1377
TscAI	CASTG	3 cut(s)	469, 612, 664
TseFI	GTSAC	1 cut(s)	601
TseI	GCWGC	9 cut(s)	152, 272, 284, 776, 1099, 1102, 1312, 1352, 1472
Tsp45I	GTSAC	1 cut(s)	601
TspDTI	ATGAA	5 cut(s)	786, 902, 1062, 1227, 1418
TspGWI	ACGGA	1 cut(s)	180
TspRI	CASTG	3 cut(s)	469, 612, 664
Van91I	CCANNNNNTGG	1 cut(s)	961
Vha464I	CTTAAG	1 cut(s)	1376
VpaK11BI	GGWCC	1 cut(s)	793
XapI	RAATTY	1 cut(s)	478
XbaI	TCTAGA	1 cut(s)	1219
XmiI	GTMKAC	2 cut(s)	543, 1420
XspI	CTAG	5 cut(s)	56, 230, 239, 1220, 1229

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.