Rroxscaffold_6G00401680

CDK5RAP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014295
Physical Location & Seq
Forward (+)
24069811 .. 24076825
7015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00401680.1

Sequence Viewer

Length: 1677 bp
ATGCCGACCGCCGATGACATTGGGAACCTCCCAATTGACATCACCTTTTCTCGTCTCGGAGAATGGCTTGTTGATCGGAAAAGAATACCGGGAGATTGGAGGAAACGATTAGCAGCCGTGAGAGCTCGGATTTCCAAGGAGTTCGCTTCCCTTCCCAAAGATATCAATCCCTATTTTCATACCTTAGACCCTGATGGAATTGGTTACTTGGAGGCCAAACAGATATATGATATTCTGGTCAAGTCTGCTCCAGAAAGTCGAAATATATTCGGCCGGCTCTCCGGTGCTGCTGGTGCTTGGGAATCAATTGTGCGTGCTTTCGAGAAGGATTATATCTATCTTGGCGAAGCTGCGCAGATAATGGTTCAAAATGTGAACTATGAGATTCCGTATCAGAAAAAGCAGGTGCAGAAGATTCAGCAACAATTAGCTGAACTAGAGCACAAGGAAGCTGATATTAAGAGAACTGCAGCTCTATCGGCAGCCAAGTATGTGGAGGCTTGTCAAGAGCTTGGATTGAAGGGAAATAATGTAAGGGCAGAACTTTTGGAGACTGCAAAATCACTTCCTACCACATTCAGCAGGATAATGGAAGTGATAAATGGTGATTCTGTGTCGAGGGCTCTGGAGTATTATTCTAACTTTGTGAGAGATGCTCACACTGAAAAGGATAAATCTTTGGTTGTGTTAACAAACTTGAAAGATTTACGTGAGCATCCCCCATCATTGGATGTTTCTGTAGACTTGGAGGTTATTGATTCTGGGAATGTTCAATCAAGCCATAATGAGTCAAACAATGTGACAGTGGATGCAGATGCTAATGCCGACAGTATAGACTGGGATTTTTCTGTGGACAGTGCTCAGATTGATTGGGATATTGGTACTGTGGAAGAAACAGAAGATGCTGGTAATGGTTTGGGCCCTTACGAAATGGTTAGTGCCAGTGAGATCTTGCAGAGTTCTCCAAATGAAGTGGCAAAATCTGATCAGGTCCCAGAGGTTTCTGTGTCCCAGATATCTTGGGATATTAGTGTTGAAACTCCTCAAGTTGATGCAATTGATGATGTCAATTTGCCGAATGTTGTTGTTGACAATCAAACATCTGTTCTTCATACTTCAACTCCAACAACTGAAATCAAGGAGGGTAGGAGCCAACTTTTGGAAACAGAGTATAGGAACAAGATTCTGGATGATCTGAATGAGATCAAAGCTTTTCTAAATCAGCGGTTGGTTGAGTTGAAGAATGAAGAAACTTTGTCATTGCAACATCAAGTCCAGGCAGTTGCTCCCTTTGTGCTGCAGCAATATGCTCATGATGCCATTGAGACAATGCTATCTGACGTTTGCTTGGCCATTTCTTTGCTGACAAATAGGAAAACGCGGGACTTGATCATGATTCTCAACTCCAAAAGATTTCTAGACAGACTAGTGAGTTCATTGGAAGAAAAGAAGCATCACGAAGTGAAGTTGAAAGAGGGTTTGAAGGGCTTAGCTACCAAACGCATGGAGCTGCACAACTCGTTATCTTCTTCCTGGCCAAAGCAGGAAGCAGCTCTAGCAAAAACACACGAGCTTAAGAAGCTGTGTGAGAGCACCCTATCCTCCATGTTTGATGGTAGACCTGTTCATATAATTGGAGAGATCAATGCCCTTTTGAATAGTGGTGTTGCTGCATAG

Protein Analysis

558

Amino Acids

62.29

Weight (kDa)

4.97

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDK5RAP3 PF05600 9 - 546 1.7e-170 CDK5 regulatory subunit-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06830
fragaria_vesca FvH4_6g22670 FvH4_6g22670
malus_domestica MD14G1060600.v1.1
prunus_persica Prupe.7G073900_v2.0.a1 Prupe.7G073900_v2.0.a1
pyrus_communis pycom14g04910
rosa_chinensis RchiOBHm_Chr3g0480241
rosa_laevigata RLG00000023508
rosa_multiflora Rmu_sc0006148.1_g000001
rosa_roxburghii Rroxscaffold_6G00401680
rosa_rugosa Rorug03G0181400
rosa_samantha Rh3AG233100 Rh3BG265700 Rh3CG263000 Rh3DG258900
rosa_wichuraiana Rw3G020940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc16I TGCGCA 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 394
AccB7I CCANNNNNTGG 1 cut(s) 1159
AccI GTMKAC 2 cut(s) 741, 1618
AccII CGCG 1 cut(s) 1381
AciI CCGC 3 cut(s) 9, 1225, 1381
AcoI YGGCCR 3 cut(s) 271, 1350, 1535
AdeI CACNNNGTG 1 cut(s) 1462
AfaI GTAC 1 cut(s) 883
AfiI CCNNNNNNNGG 2 cut(s) 727, 1159
AflII CTTAAG 1 cut(s) 1574
AhlI ACTAGT 1 cut(s) 1426
AjnI CCWGG 2 cut(s) 1275, 1532
Alw21I GWGCWC 4 cut(s) 127, 444, 862, 1595
Alw26I GTCTC 3 cut(s) 59, 545, 1319
AoxI GGCC 5 cut(s) 213, 271, 919, 1350, 1535
ApaI GGGCCC 1 cut(s) 923
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 3 cut(s) 919, 920, 991
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 2 cut(s) 34, 617
AvaII GGWCC 1 cut(s) 991
BaeGI GKGCMC 1 cut(s) 923
BalI TGGCCA 2 cut(s) 1352, 1537
BanII GRGCYC 3 cut(s) 127, 625, 923
BauI CACGAG 1 cut(s) 1568
Bbv12I GWGCWC 4 cut(s) 127, 444, 862, 1595
BccI CCATC 3 cut(s) 188, 730, 1607
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 459, 493
BciT130I CCWGG 2 cut(s) 1277, 1534
BclI TGATCA 2 cut(s) 985, 1389
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 3 cut(s) 59, 545, 1319
BcuI ACTAGT 1 cut(s) 1426
BfaI CTAG 4 cut(s) 437, 1418, 1427, 1556
BfmI CTRYAG 3 cut(s) 468, 738, 1298
BfrI CTTAAG 1 cut(s) 1574
BfuAI ACCTGC 1 cut(s) 394
BglII AGATCT 1 cut(s) 948
BlpI GCTNAGC 1 cut(s) 1489
Bme1390I CCNGG 3 cut(s) 90, 1277, 1534
Bme18I GGWCC 1 cut(s) 991
BmgT120I GGNCC 3 cut(s) 919, 920, 991
BmiI GGNNCC 4 cut(s) 26, 921, 993, 1151
BmrFI CCNGG 3 cut(s) 90, 1277, 1534
BmrI ACTGGG 1 cut(s) 847
BmsI GCATC 8 cut(s) 643, 724, 799, 805, 892, 1042, 1306, 1462
BmuI ACTGGG 1 cut(s) 847
BplI GAGNNNNNCTC 2 cut(s) 640, 672
BpmI CTGGAG 2 cut(s) 234, 647
Bpu1102I GCTNAGC 1 cut(s) 1489
BpuEI CTTGAG 1 cut(s) 1029
BpuMI CCSGG 1 cut(s) 90
BsaAI YACGTR 1 cut(s) 710
BsaBI GATNNNNATC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 1105, 1135
Bsc4I CCNNNNNNNGG 2 cut(s) 727, 1159
Bse118I RCCGGY 1 cut(s) 273
Bse1I ACTGG 2 cut(s) 842, 942
Bse3DI GCAATG 1 cut(s) 1259
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 2 cut(s) 1277, 1534
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 4 cut(s) 715, 736, 814, 1195
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 727, 1159
BseMI GCAATG 1 cut(s) 1259
BseMII CTCAG 1 cut(s) 875
BseNI ACTGG 2 cut(s) 842, 942
BseRI GAGGAG 1 cut(s) 1032
BseSI GKGCMC 1 cut(s) 923
BseX3I CGGCCG 1 cut(s) 271
BsgI GTGCAG 2 cut(s) 428, 1496
Bsh1236I CGCG 1 cut(s) 1381
Bsh1285I CGRYCG 2 cut(s) 9, 274
BshFI GGCC 5 cut(s) 215, 273, 921, 1352, 1537
BsiEI CGRYCG 2 cut(s) 9, 274
BsiHKAI GWGCWC 4 cut(s) 127, 444, 862, 1595
BsiSI CCGG 3 cut(s) 89, 274, 282
BslFI GGGAC 3 cut(s) 977, 994, 1397
BslI CCNNNNNNNGG 2 cut(s) 727, 1159
BsmAI GTCTC 3 cut(s) 59, 545, 1319
BsmBI CGTCTC 1 cut(s) 59
BsmFI GGGAC 3 cut(s) 977, 994, 1397
BsnI GGCC 5 cut(s) 215, 273, 921, 1352, 1537
Bsp120I GGGCCC 1 cut(s) 919
Bsp1286I GDGCHC 6 cut(s) 127, 444, 625, 862, 923, 1595
Bsp143I GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
Bsp1720I GCTNAGC 1 cut(s) 1489
BspACI CCGC 3 cut(s) 9, 1225, 1381
BspANI GGCC 5 cut(s) 215, 273, 921, 1352, 1537
BspCNI CTCAG 1 cut(s) 874
BspFNI CGCG 1 cut(s) 1381
BspHI TCATGA 2 cut(s) 1312, 1392
BspLI GGNNCC 4 cut(s) 26, 921, 993, 1151
BspMAI CTGCAG 2 cut(s) 472, 1302
BspMI ACCTGC 1 cut(s) 394
BspTI CTTAAG 1 cut(s) 1574
BsrDI GCAATG 1 cut(s) 1259
BsrFI RCCGGY 1 cut(s) 273
BsrI ACTGG 2 cut(s) 842, 942
BssAI RCCGGY 1 cut(s) 273
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
BssSI CACGAG 1 cut(s) 1568
BssT1I CCWWGG 1 cut(s) 135
Bst2BI CACGAG 1 cut(s) 1568
Bst2UI CCWGG 2 cut(s) 1277, 1534
Bst4CI ACNGT 4 cut(s) 805, 830, 857, 886
BstAFI CTTAAG 1 cut(s) 1574
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 275, 315
BstDEI CTNAG 3 cut(s) 184, 861, 1489
BstF5I GGATG 4 cut(s) 715, 736, 814, 1195
BstFNI CGCG 1 cut(s) 1381
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 7 cut(s) 76, 951, 988, 1195, 1206, 1392, 1644
BstMAI GTCTC 3 cut(s) 59, 545, 1319
BstMBI GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
BstMCI CGRYCG 2 cut(s) 9, 274
BstMWI GCNNNNNNNGC 6 cut(s) 122, 293, 479, 1316, 1556, 1579
BstNI CCWGG 2 cut(s) 1277, 1534
BstSCI CCNGG 3 cut(s) 88, 1275, 1532
BstSFI CTRYAG 3 cut(s) 468, 738, 1298
BstSLI GKGCMC 1 cut(s) 923
BstUI CGCG 1 cut(s) 1381
BstX2I RGATCY 1 cut(s) 948
BstXI CCANNNNNNTGG 2 cut(s) 493, 1504
BstYI RGATCY 1 cut(s) 948
BstZI CGGCCG 1 cut(s) 271
BsuRI GGCC 5 cut(s) 215, 273, 921, 1352, 1537
BtsCI GGATG 4 cut(s) 715, 736, 814, 1195
BtsIMutI CAGTG 4 cut(s) 660, 810, 862, 949
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 275, 315
CciI TCATGA 2 cut(s) 1312, 1392
CfoI GCGC 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 273
Cfr13I GGNCC 3 cut(s) 919, 920, 991
Csp6I GTAC 1 cut(s) 882
CspCI CAANNNNNGTGG 2 cut(s) 954, 989
CviAII CATG 4 cut(s) 1313, 1393, 1504, 1606
CviQI GTAC 1 cut(s) 882
DdeI CTNAG 3 cut(s) 184, 861, 1489
DpnI GATC 7 cut(s) 75, 950, 987, 1194, 1205, 1391, 1643
DpnII GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
DraIII CACNNNGTG 1 cut(s) 1462
EaeI YGGCCR 3 cut(s) 271, 1350, 1535
EagI CGGCCG 1 cut(s) 271
Ecl136II GAGCTC 1 cut(s) 125
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 135
Eco24I GRGCYC 3 cut(s) 127, 625, 923
Eco32I GATATC 2 cut(s) 163, 1017
Eco47I GGWCC 1 cut(s) 991
Eco52I CGGCCG 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 125
EcoO109I RGGNCCY 2 cut(s) 920, 991
EcoRII CCWGG 2 cut(s) 1275, 1532
EcoRV GATATC 2 cut(s) 163, 1017
EcoT14I CCWWGG 1 cut(s) 135
EcoT38I GRGCYC 3 cut(s) 127, 625, 923
ErhI CCWWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 4 cut(s) 1316, 1396, 1507, 1609
FaqI GGGAC 3 cut(s) 977, 994, 1397
FatI CATG 4 cut(s) 1312, 1392, 1503, 1605
FauI CCCGC 1 cut(s) 1374
FbaI TGATCA 2 cut(s) 985, 1389
FblI GTMKAC 2 cut(s) 741, 1618
FokI GGATG 4 cut(s) 702, 743, 821, 1202
FriOI GRGCYC 3 cut(s) 127, 625, 923
FspBI CTAG 4 cut(s) 437, 1418, 1427, 1556
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 1 cut(s) 354
GsuI CTGGAG 2 cut(s) 234, 647
HaeIII GGCC 5 cut(s) 215, 273, 921, 1352, 1537
HapII CCGG 3 cut(s) 89, 274, 282
HhaI GCGC 1 cut(s) 355
Hin1II CATG 4 cut(s) 1316, 1396, 1507, 1609
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 2 cut(s) 690, 1090
HindII GTYRAC 2 cut(s) 690, 1090
HindIII AAGCTT 1 cut(s) 1209
HinfI GANTC 8 cut(s) 302, 385, 415, 608, 758, 788, 1183, 1396
HpaI GTTAAC 1 cut(s) 690
HpaII CCGG 3 cut(s) 89, 274, 282
HphI GGTGA 2 cut(s) 34, 617
Hpy166II GTNNAC 6 cut(s) 376, 690, 742, 853, 1090, 1619
Hpy188I TCNGA 8 cut(s) 59, 78, 129, 396, 864, 985, 1197, 1339
Hpy188III TCNNGA 9 cut(s) 251, 322, 506, 626, 1187, 1313, 1393, 1418, 1457
Hpy8I GTNNAC 6 cut(s) 376, 690, 742, 853, 1090, 1619
HpyAV CCTTC 4 cut(s) 161, 319, 514, 1477
HpyCH4III ACNGT 4 cut(s) 805, 830, 857, 886
HpyCH4IV ACGT 2 cut(s) 709, 1341
HpyF10VI GCNNNNNNNGC 6 cut(s) 122, 293, 479, 1316, 1556, 1579
HpyF3I CTNAG 3 cut(s) 184, 861, 1489
HpySE526I ACGT 2 cut(s) 709, 1341
Hsp92II CATG 4 cut(s) 1316, 1396, 1507, 1609
HspAI GCGC 1 cut(s) 353
KroI GCCGGC 1 cut(s) 273
KroNI GCCGGC 1 cut(s) 275
Ksp22I TGATCA 2 cut(s) 985, 1389
KspAI GTTAAC 1 cut(s) 690
Kzo9I GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
LmnI GCTCC 4 cut(s) 253, 1149, 1291, 1507
LweI GCATC 8 cut(s) 643, 724, 799, 805, 892, 1042, 1306, 1462
MaeI CTAG 4 cut(s) 437, 1418, 1427, 1556
MaeII ACGT 2 cut(s) 709, 1341
MaeIII GTNAC 2 cut(s) 203, 799
MalI GATC 7 cut(s) 75, 950, 987, 1194, 1205, 1391, 1643
MboI GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
MboII GAAGA 9 cut(s) 424, 902, 911, 1100, 1252, 1259, 1454, 1518, 1521
MfeI CAATTG 3 cut(s) 33, 306, 1056
MflI RGATCY 1 cut(s) 948
MhlI GDGCHC 6 cut(s) 127, 444, 625, 862, 923, 1595
MlsI TGGCCA 2 cut(s) 1352, 1537
MluCI AATT 7 cut(s) 33, 198, 306, 425, 1056, 1069, 1632
MluNI TGGCCA 2 cut(s) 1352, 1537
MlyI GAGTC 1 cut(s) 797
MmeI TCCRAC 1 cut(s) 1148
Mox20I TGGCCA 2 cut(s) 1352, 1537
MroNI GCCGGC 1 cut(s) 273
MscI TGGCCA 2 cut(s) 1352, 1537
MseI TTAA 3 cut(s) 459, 689, 1575
Msp20I TGGCCA 2 cut(s) 1352, 1537
MspA1I CMGCKG 1 cut(s) 1225
MspCI CTTAAG 1 cut(s) 1574
MspI CCGG 3 cut(s) 89, 274, 282
MspR9I CCNGG 3 cut(s) 90, 1277, 1534
MunI CAATTG 3 cut(s) 33, 306, 1056
MvaI CCWGG 2 cut(s) 1277, 1534
MvnI CGCG 1 cut(s) 1381
MwoI GCNNNNNNNGC 6 cut(s) 122, 293, 479, 1316, 1556, 1579
NaeI GCCGGC 1 cut(s) 275
NciI CCSGG 1 cut(s) 90
NdeII GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
NgoMIV GCCGGC 1 cut(s) 273
NlaIII CATG 4 cut(s) 1316, 1396, 1507, 1609
NlaIV GGNNCC 4 cut(s) 26, 921, 993, 1151
NmuCI GTSAC 1 cut(s) 799
NsbI TGCGCA 1 cut(s) 354
PagI TCATGA 2 cut(s) 1312, 1392
PaqCI CACCTGC 1 cut(s) 394
PdiI GCCGGC 1 cut(s) 275
PfeI GAWTC 7 cut(s) 302, 385, 415, 608, 758, 1183, 1396
PflMI CCANNNNNTGG 1 cut(s) 1159
PleI GAGTC 1 cut(s) 796
PpsI GAGTC 1 cut(s) 796
Ppu21I YACGTR 1 cut(s) 710
PpuMI RGGWCCY 1 cut(s) 991
Psp124BI GAGCTC 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 991
Psp6I CCWGG 2 cut(s) 1275, 1532
PspGI CCWGG 2 cut(s) 1275, 1532
PspN4I GGNNCC 4 cut(s) 26, 921, 993, 1151
PspOMI GGGCCC 1 cut(s) 919
PspPI GGNCC 3 cut(s) 919, 920, 991
PspPPI RGGWCCY 1 cut(s) 991
PstI CTGCAG 2 cut(s) 472, 1302
PsuI RGATCY 1 cut(s) 948
RsaI GTAC 1 cut(s) 883
RsaNI GTAC 1 cut(s) 882
SacI GAGCTC 1 cut(s) 127
SaqAI TTAA 3 cut(s) 459, 689, 1575
Sau3AI GATC 7 cut(s) 73, 948, 985, 1192, 1203, 1389, 1641
Sau96I GGNCC 3 cut(s) 919, 920, 991
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 3 cut(s) 90, 1277, 1534
SduI GDGCHC 6 cut(s) 127, 444, 625, 862, 923, 1595
SfaNI GCATC 8 cut(s) 643, 724, 799, 805, 892, 1042, 1306, 1462
SfcI CTRYAG 3 cut(s) 468, 738, 1298
SinI GGWCC 1 cut(s) 991
SmlI CTYRAG 2 cut(s) 1044, 1574
SmoI CTYRAG 2 cut(s) 1044, 1574
SpeI ACTAGT 1 cut(s) 1426
Sse9I AATT 7 cut(s) 33, 198, 306, 425, 1056, 1069, 1632
SsiI CCGC 3 cut(s) 9, 1225, 1381
SspMI CTAG 4 cut(s) 437, 1418, 1427, 1556
SstI GAGCTC 1 cut(s) 127
StyD4I CCNGG 3 cut(s) 88, 1275, 1532
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 4 cut(s) 805, 830, 857, 886
TaiI ACGT 2 cut(s) 712, 1344
TaqI TCGA 3 cut(s) 259, 321, 617
TasI AATT 7 cut(s) 33, 198, 306, 425, 1056, 1069, 1632
TfiI GAWTC 7 cut(s) 302, 385, 415, 608, 758, 1183, 1396
Tru1I TTAA 3 cut(s) 459, 689, 1575
Tru9I TTAA 3 cut(s) 459, 689, 1575
TscAI CASTG 4 cut(s) 667, 810, 862, 949
TseFI GTSAC 1 cut(s) 799
Tsp45I GTSAC 1 cut(s) 799
TspDTI ATGAA 6 cut(s) 167, 984, 1100, 1260, 1425, 1616
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 4 cut(s) 667, 810, 862, 949
Van91I CCANNNNNTGG 1 cut(s) 1159
Vha464I CTTAAG 1 cut(s) 1574
VpaK11BI GGWCC 1 cut(s) 991
XbaI TCTAGA 1 cut(s) 1417
XmiI GTMKAC 2 cut(s) 741, 1618
XspI CTAG 4 cut(s) 437, 1418, 1427, 1556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.