Rh3BG265700

CDK5RAP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0014295
Physical Location & Seq
Reverse (-)
25687139 .. 25698367
11229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG265700.1

Sequence Viewer

Length: 1497 bp
ATGTTTAGGAGTCTTTCACAGTTTCAAGTTTCAACTCTTATCACAGAGAGAAGAGAAGAGAGAGGCTTGGTTCCCCTGAAAATGCCGACCGCCGATGACATTGGGAACCTCCCAATTGACATCACCTTTTCTCGTCTCGGAGAATGGCTTGTTGATCGGAAAAGAATACCGGGAGATTGGAGGAAACGATTGGCAGCCGTGAGAGCTCGGATTTCCAAGGAGTTCGCTTCACTTCCCAAAGATATCAATCCCTATTTTCATACCTTAGACCCTGATGGAATTGGCTACTTAGAGGCCAAGCAGATATATGATATTCTGGTCAAGTCTGCTCCAGAAAGTCGAAATATATTCGGCCGGCTCTCCGGTGCTGCTGGTACTTGGGAATCAATTGTGCGTGCCTTCGAGAAGGATTATATCTATCTTGGCGAAGCTGCGCAGATAATGGTTCAAAATGTGAACTATGAGATTCCGTATCAGAAAAAGCAGGTGCAGAAGATTCAGCAACAACTAGCTGAACTAGAGCACAAGGAAGCTGATATTAAGAGAACTGCAGCTCTATCGGCAGCCAAGTATGTGGAGGCTTGTCAAGAGCTTGGGTTGAAGGGAAATAATGTAAGGGCGGAACTTTTAGAGACTGCAAAATCACTTCCTACCACATTCAGCAGGATATTGGAAGTGATAAATGGTGATTCTGTGTCGAGGGCTCTGGAGTATTATTCTAACTTTGTGAGAGATGCTCACACTGAAAAGGATAAATCTTTGGTTGTGTTAACAAACTTGAAAGATTTACGTGAGCATCCCCCATCATTGGATGTTTCTGTAGACTTAGAGTTTGTTGATTCTGGGAATGTTCAATCAAGCCATAATGAGTCAAACAATGTGACAGTGGATGCAGATGCTAATGCTGACAGTATAGACTGGGATTTTTCTGTGGACAGTGCTCAGATTGATTGGGATATTGGTACTGTGGAAGAAACAGAAGATGCTGGTAACGGTTTGGGCCCTTACGAAATGGTTAGTGCCAGTGAGATCTTGCAGAGTTCTCCAAATGAAGTGGCAAAATCTGATCAGGTCCCAGAGGTTTCTGTGTCCGAGATATCTTGGGATATTAGTGTTGAAACTCCTCAAGTTGATGCAATTGATGATGTCAATTTGCCGAATGTTGTTGTTGACAATCAAACATCTGTTCTTCATACTTCAACTCCAACAACTGAAATCAAGGAAGGTAGGAGCCAACTTTTGGAAACAGAGTATAGGAACAAGATTCTGGATGATCTGAATGAGATCAAAGCTTTTCTAAATCAGCGGTTGGTTGAGTTGAAGAATGAAGAAACTTTGTCATTGCAACATCAAGTCCAGGCAGTTGCTCCCTTTGTGCTGCAGCAATATGCTCATGATGCCATTGAGACAATGCTATCTGATGTTTGCTTGGCCATTTCTTTGCTGACAAATAGGAAAACGCGGGACCTGATCATGATTCTCAACTCCAAAAGGTAA

Protein Analysis

498

Amino Acids

55.92

Weight (kDa)

4.75

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDK5RAP3 PF05600 36 - 498 1.7e-140 CDK5 regulatory subunit-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06830
fragaria_vesca FvH4_6g22670 FvH4_6g22670
malus_domestica MD14G1060600.v1.1
prunus_persica Prupe.7G073900_v2.0.a1 Prupe.7G073900_v2.0.a1
pyrus_communis pycom14g04910
rosa_chinensis RchiOBHm_Chr3g0480241
rosa_laevigata RLG00000023508
rosa_multiflora Rmu_sc0006148.1_g000001
rosa_roxburghii Rroxscaffold_6G00401680
rosa_rugosa Rorug03G0181400
rosa_samantha Rh3AG233100 Rh3BG265700 Rh3CG263000 Rh3DG258900
rosa_wichuraiana Rw3G020940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 475
Acc16I TGCGCA 1 cut(s) 435
Acc36I ACCTGC 1 cut(s) 475
AccB7I CCANNNNNTGG 1 cut(s) 1240
AccI GTMKAC 1 cut(s) 822
AccII CGCG 1 cut(s) 1462
AciI CCGC 4 cut(s) 90, 620, 1306, 1462
AcoI YGGCCR 2 cut(s) 352, 1431
AfaI GTAC 2 cut(s) 376, 964
AfiI CCNNNNNNNGG 2 cut(s) 808, 1240
AgsI TTSAA 9 cut(s) 26, 33, 449, 601, 781, 854, 1118, 1200, 1321
AjnI CCWGG 1 cut(s) 1356
AluBI AGCT 7 cut(s) 206, 431, 512, 533, 554, 592, 1292
AluI AGCT 7 cut(s) 206, 431, 512, 533, 554, 592, 1292
Alw21I GWGCWC 3 cut(s) 208, 525, 943
Alw26I GTCTC 3 cut(s) 140, 626, 1400
AoxI GGCC 4 cut(s) 294, 352, 1000, 1431
ApaI GGGCCC 1 cut(s) 1004
ApeKI GCWGC 7 cut(s) 194, 368, 431, 551, 563, 1378, 1381
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 4 cut(s) 1000, 1001, 1072, 1465
AsuC2I CCSGG 1 cut(s) 171
AsuHPI GGTGA 2 cut(s) 115, 698
AvaII GGWCC 2 cut(s) 1072, 1465
BaeGI GKGCMC 1 cut(s) 1004
BalI TGGCCA 1 cut(s) 1433
BanII GRGCYC 3 cut(s) 208, 706, 1004
Bbv12I GWGCWC 3 cut(s) 208, 525, 943
BbvI GCAGC 7 cut(s) 206, 355, 418, 563, 575, 1365, 1393
BccI CCATC 2 cut(s) 269, 811
BceAI ACGGC 1 cut(s) 182
BcgI CGANNNNNNTGC 2 cut(s) 540, 574
BciT130I CCWGG 1 cut(s) 1358
BclI TGATCA 2 cut(s) 1066, 1470
BcnI CCSGG 1 cut(s) 171
BcoDI GTCTC 3 cut(s) 140, 626, 1400
BfaI CTAG 2 cut(s) 509, 518
BfmI CTRYAG 3 cut(s) 549, 819, 1379
BfuAI ACCTGC 1 cut(s) 475
BglII AGATCT 1 cut(s) 1029
BisI GCNGC 7 cut(s) 195, 369, 432, 552, 564, 1379, 1382
BlsI GCNGC 7 cut(s) 196, 370, 433, 553, 565, 1380, 1383
Bme1390I CCNGG 2 cut(s) 171, 1358
Bme18I GGWCC 2 cut(s) 1072, 1465
BmgT120I GGNCC 4 cut(s) 1000, 1001, 1072, 1465
BmiI GGNNCC 6 cut(s) 72, 107, 1002, 1074, 1232, 1466
BmrFI CCNGG 2 cut(s) 171, 1358
BmrI ACTGGG 1 cut(s) 928
BmsI GCATC 7 cut(s) 724, 805, 880, 886, 973, 1123, 1387
BmuI ACTGGG 1 cut(s) 928
BplI GAGNNNNNCTC 2 cut(s) 721, 753
BpmI CTGGAG 2 cut(s) 315, 728
BpuEI CTTGAG 1 cut(s) 1110
BpuMI CCSGG 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 791
BsaBI GATNNNNATC 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 362
BsaXI ACNNNNNCTCC 2 cut(s) 1186, 1216
Bsc4I CCNNNNNNNGG 2 cut(s) 808, 1240
Bse118I RCCGGY 1 cut(s) 354
Bse1I ACTGG 2 cut(s) 923, 1023
Bse3DI GCAATG 1 cut(s) 1340
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 1 cut(s) 1358
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 4 cut(s) 796, 817, 895, 1276
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 2 cut(s) 808, 1240
BseMI GCAATG 1 cut(s) 1340
BseMII CTCAG 1 cut(s) 956
BseNI ACTGG 2 cut(s) 923, 1023
BseRI GAGGAG 1 cut(s) 1113
BseSI GKGCMC 1 cut(s) 1004
BseX3I CGGCCG 1 cut(s) 352
BseXI GCAGC 7 cut(s) 206, 355, 418, 563, 575, 1365, 1393
BsgI GTGCAG 1 cut(s) 509
Bsh1236I CGCG 1 cut(s) 1462
Bsh1285I CGRYCG 2 cut(s) 90, 355
BshFI GGCC 4 cut(s) 296, 354, 1002, 1433
BsiEI CGRYCG 2 cut(s) 90, 355
BsiHKAI GWGCWC 3 cut(s) 208, 525, 943
BsiSI CCGG 3 cut(s) 170, 355, 363
BslFI GGGAC 2 cut(s) 1058, 1478
BslI CCNNNNNNNGG 2 cut(s) 808, 1240
BsmAI GTCTC 3 cut(s) 140, 626, 1400
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 2 cut(s) 1058, 1478
BsnI GGCC 4 cut(s) 296, 354, 1002, 1433
Bsp120I GGGCCC 1 cut(s) 1000
Bsp1286I GDGCHC 5 cut(s) 208, 525, 706, 943, 1004
Bsp143I GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
BspACI CCGC 4 cut(s) 90, 620, 1306, 1462
BspANI GGCC 4 cut(s) 296, 354, 1002, 1433
BspCNI CTCAG 1 cut(s) 955
BspFNI CGCG 1 cut(s) 1462
BspHI TCATGA 2 cut(s) 1393, 1473
BspLI GGNNCC 6 cut(s) 72, 107, 1002, 1074, 1232, 1466
BspMAI CTGCAG 2 cut(s) 553, 1383
BspMI ACCTGC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 1340
BsrFI RCCGGY 1 cut(s) 354
BsrI ACTGG 2 cut(s) 923, 1023
BssAI RCCGGY 1 cut(s) 354
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 1358
Bst4CI ACNGT 6 cut(s) 21, 886, 911, 938, 967, 995
Bst6I CTCTTC 2 cut(s) 46, 51
BstBAI YACGTR 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 356, 396
BstDEI CTNAG 4 cut(s) 265, 289, 826, 942
BstF5I GGATG 4 cut(s) 796, 817, 895, 1276
BstFNI CGCG 1 cut(s) 1462
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 6 cut(s) 157, 1032, 1069, 1276, 1287, 1473
BstMAI GTCTC 3 cut(s) 140, 626, 1400
BstMBI GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
BstMCI CGRYCG 2 cut(s) 90, 355
BstMWI GCNNNNNNNGC 3 cut(s) 203, 560, 1397
BstNI CCWGG 1 cut(s) 1358
BstSCI CCNGG 2 cut(s) 169, 1356
BstSFI CTRYAG 3 cut(s) 549, 819, 1379
BstSLI GKGCMC 1 cut(s) 1004
BstUI CGCG 1 cut(s) 1462
BstV1I GCAGC 7 cut(s) 206, 355, 418, 563, 575, 1365, 1393
BstX2I RGATCY 1 cut(s) 1029
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 1 cut(s) 1029
BstZI CGGCCG 1 cut(s) 352
BsuRI GGCC 4 cut(s) 296, 354, 1002, 1433
BtsCI GGATG 4 cut(s) 796, 817, 895, 1276
BtsIMutI CAGTG 4 cut(s) 741, 891, 943, 1030
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 356, 396
CciI TCATGA 2 cut(s) 1393, 1473
CfoI GCGC 1 cut(s) 436
Cfr10I RCCGGY 1 cut(s) 354
Cfr13I GGNCC 4 cut(s) 1000, 1001, 1072, 1465
Csp6I GTAC 2 cut(s) 375, 963
CspCI CAANNNNNGTGG 2 cut(s) 1035, 1070
CviAII CATG 2 cut(s) 1394, 1474
CviQI GTAC 2 cut(s) 375, 963
DdeI CTNAG 4 cut(s) 265, 289, 826, 942
DpnI GATC 6 cut(s) 156, 1031, 1068, 1275, 1286, 1472
DpnII GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
EaeI YGGCCR 2 cut(s) 352, 1431
EagI CGGCCG 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 46, 51
EarI CTCTTC 2 cut(s) 46, 51
EciI GGCGGA 1 cut(s) 635
Ecl136II GAGCTC 1 cut(s) 206
EclXI CGGCCG 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 3 cut(s) 208, 706, 1004
Eco32I GATATC 2 cut(s) 244, 1098
Eco47I GGWCC 2 cut(s) 1072, 1465
Eco52I CGGCCG 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 206
EcoO109I RGGNCCY 3 cut(s) 1001, 1072, 1465
EcoRII CCWGG 1 cut(s) 1356
EcoRV GATATC 2 cut(s) 244, 1098
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 3 cut(s) 208, 706, 1004
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 2 cut(s) 1397, 1477
FaqI GGGAC 2 cut(s) 1058, 1478
FatI CATG 2 cut(s) 1393, 1473
FauI CCCGC 1 cut(s) 1455
FbaI TGATCA 2 cut(s) 1066, 1470
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 7 cut(s) 195, 369, 432, 552, 564, 1379, 1382
FokI GGATG 4 cut(s) 783, 824, 902, 1283
FriOI GRGCYC 3 cut(s) 208, 706, 1004
Fsp4HI GCNGC 7 cut(s) 195, 369, 432, 552, 564, 1379, 1382
FspBI CTAG 2 cut(s) 509, 518
FspI TGCGCA 1 cut(s) 435
GlaI GCGC 1 cut(s) 435
GluI GCNGC 7 cut(s) 195, 369, 432, 552, 564, 1379, 1382
GsuI CTGGAG 2 cut(s) 315, 728
HaeIII GGCC 4 cut(s) 296, 354, 1002, 1433
HapII CCGG 3 cut(s) 170, 355, 363
HhaI GCGC 1 cut(s) 436
Hin1II CATG 2 cut(s) 1397, 1477
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HincII GTYRAC 2 cut(s) 771, 1171
HindII GTYRAC 2 cut(s) 771, 1171
HindIII AAGCTT 1 cut(s) 1290
HinfI GANTC 9 cut(s) 10, 383, 466, 496, 689, 839, 869, 1264, 1477
HpaI GTTAAC 1 cut(s) 771
HpaII CCGG 3 cut(s) 170, 355, 363
HphI GGTGA 2 cut(s) 115, 698
Hpy166II GTNNAC 5 cut(s) 457, 771, 823, 934, 1171
Hpy188I TCNGA 9 cut(s) 140, 159, 210, 477, 945, 1066, 1093, 1278, 1420
Hpy188III TCNNGA 7 cut(s) 332, 403, 587, 707, 1268, 1394, 1474
Hpy8I GTNNAC 5 cut(s) 457, 771, 823, 934, 1171
HpyAV CCTTC 4 cut(s) 400, 409, 595, 1217
HpyCH4III ACNGT 6 cut(s) 21, 886, 911, 938, 967, 995
HpyCH4IV ACGT 1 cut(s) 790
HpyCH4V TGCA 8 cut(s) 490, 551, 638, 893, 1036, 1136, 1345, 1381
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 560, 1397
HpyF3I CTNAG 4 cut(s) 265, 289, 826, 942
HpySE526I ACGT 1 cut(s) 790
Hsp92II CATG 2 cut(s) 1397, 1477
HspAI GCGC 1 cut(s) 434
KroI GCCGGC 1 cut(s) 354
KroNI GCCGGC 1 cut(s) 356
Ksp22I TGATCA 2 cut(s) 1066, 1470
KspAI GTTAAC 1 cut(s) 771
Kzo9I GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
LmnI GCTCC 3 cut(s) 334, 1230, 1372
Lsp1109I GCAGC 7 cut(s) 206, 355, 418, 563, 575, 1365, 1393
LweI GCATC 7 cut(s) 724, 805, 880, 886, 973, 1123, 1387
MaeI CTAG 2 cut(s) 509, 518
MaeII ACGT 1 cut(s) 790
MaeIII GTNAC 2 cut(s) 880, 989
MalI GATC 6 cut(s) 156, 1031, 1068, 1275, 1286, 1472
MboI GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
MboII GAAGA 8 cut(s) 63, 68, 505, 983, 992, 1181, 1333, 1340
MfeI CAATTG 3 cut(s) 114, 387, 1137
MflI RGATCY 1 cut(s) 1029
MhlI GDGCHC 5 cut(s) 208, 525, 706, 943, 1004
MlsI TGGCCA 1 cut(s) 1433
MluCI AATT 5 cut(s) 114, 279, 387, 1137, 1150
MluNI TGGCCA 1 cut(s) 1433
MlyI GAGTC 2 cut(s) 19, 878
MmeI TCCRAC 1 cut(s) 1229
MnlI CCTC 8 cut(s) 56, 119, 174, 286, 571, 693, 1072, 1134
Mox20I TGGCCA 1 cut(s) 1433
MroNI GCCGGC 1 cut(s) 354
MscI TGGCCA 1 cut(s) 1433
MseI TTAA 2 cut(s) 540, 770
Msp20I TGGCCA 1 cut(s) 1433
MspA1I CMGCKG 1 cut(s) 1306
MspI CCGG 3 cut(s) 170, 355, 363
MspR9I CCNGG 2 cut(s) 171, 1358
MunI CAATTG 3 cut(s) 114, 387, 1137
MvaI CCWGG 1 cut(s) 1358
MvnI CGCG 1 cut(s) 1462
MwoI GCNNNNNNNGC 3 cut(s) 203, 560, 1397
NaeI GCCGGC 1 cut(s) 356
NciI CCSGG 1 cut(s) 171
NdeII GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
NgoMIV GCCGGC 1 cut(s) 354
NlaIII CATG 2 cut(s) 1397, 1477
NlaIV GGNNCC 6 cut(s) 72, 107, 1002, 1074, 1232, 1466
NmuCI GTSAC 1 cut(s) 880
NsbI TGCGCA 1 cut(s) 435
PagI TCATGA 2 cut(s) 1393, 1473
PaqCI CACCTGC 1 cut(s) 475
PdiI GCCGGC 1 cut(s) 356
PfeI GAWTC 7 cut(s) 383, 466, 496, 689, 839, 1264, 1477
PflMI CCANNNNNTGG 1 cut(s) 1240
PkrI GCNGC 7 cut(s) 196, 370, 433, 553, 565, 1380, 1383
PleI GAGTC 2 cut(s) 18, 877
PpsI GAGTC 2 cut(s) 18, 877
Ppu21I YACGTR 1 cut(s) 791
PpuMI RGGWCCY 2 cut(s) 1072, 1465
Psp124BI GAGCTC 1 cut(s) 208
Psp5II RGGWCCY 2 cut(s) 1072, 1465
Psp6I CCWGG 1 cut(s) 1356
PspGI CCWGG 1 cut(s) 1356
PspN4I GGNNCC 6 cut(s) 72, 107, 1002, 1074, 1232, 1466
PspOMI GGGCCC 1 cut(s) 1000
PspPI GGNCC 4 cut(s) 1000, 1001, 1072, 1465
PspPPI RGGWCCY 2 cut(s) 1072, 1465
PstI CTGCAG 2 cut(s) 553, 1383
PsuI RGATCY 1 cut(s) 1029
RsaI GTAC 2 cut(s) 376, 964
RsaNI GTAC 2 cut(s) 375, 963
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 2 cut(s) 540, 770
SatI GCNGC 7 cut(s) 195, 369, 432, 552, 564, 1379, 1382
Sau3AI GATC 6 cut(s) 154, 1029, 1066, 1273, 1284, 1470
Sau96I GGNCC 4 cut(s) 1000, 1001, 1072, 1465
SchI GAGTC 2 cut(s) 19, 878
ScrFI CCNGG 2 cut(s) 171, 1358
SduI GDGCHC 5 cut(s) 208, 525, 706, 943, 1004
SfaNI GCATC 7 cut(s) 724, 805, 880, 886, 973, 1123, 1387
SfcI CTRYAG 3 cut(s) 549, 819, 1379
SinI GGWCC 2 cut(s) 1072, 1465
SmlI CTYRAG 1 cut(s) 1125
SmoI CTYRAG 1 cut(s) 1125
Sse9I AATT 5 cut(s) 114, 279, 387, 1137, 1150
SsiI CCGC 4 cut(s) 90, 620, 1306, 1462
SspMI CTAG 2 cut(s) 509, 518
SstI GAGCTC 1 cut(s) 208
StyD4I CCNGG 2 cut(s) 169, 1356
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 6 cut(s) 21, 886, 911, 938, 967, 995
TaiI ACGT 1 cut(s) 793
TaqI TCGA 3 cut(s) 340, 402, 698
TasI AATT 5 cut(s) 114, 279, 387, 1137, 1150
TfiI GAWTC 7 cut(s) 383, 466, 496, 689, 839, 1264, 1477
Tru1I TTAA 2 cut(s) 540, 770
Tru9I TTAA 2 cut(s) 540, 770
TscAI CASTG 4 cut(s) 748, 891, 943, 1030
TseFI GTSAC 1 cut(s) 880
TseI GCWGC 7 cut(s) 194, 368, 431, 551, 563, 1378, 1381
Tsp45I GTSAC 1 cut(s) 880
TspDTI ATGAA 4 cut(s) 248, 1065, 1181, 1341
TspGWI ACGGA 1 cut(s) 459
TspRI CASTG 4 cut(s) 748, 891, 943, 1030
Van91I CCANNNNNTGG 1 cut(s) 1240
VpaK11BI GGWCC 2 cut(s) 1072, 1465
XmiI GTMKAC 1 cut(s) 822
XspI CTAG 2 cut(s) 509, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.