RLG00000024027

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018053
Physical Location & Seq
Reverse (-)
30962156 .. 30963363
1208 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024027

Sequence Viewer

Length: 831 bp
ATGTGGAGAGGAATTCGTCTACAACTCTGCCTCTTACTAGCCTACCCCATTCTCTGGTTCATCTTTTGTCCCAAAGATCCCAAATTCATCATCACAGATGCCTCTCTAACCCAATTCAATTTCACCAACGACAACAAGACCCTCGACTACAACCTTGCGCTCAACATCACCATCACCAACCCCAACAGAAAGGCTGCCATATTCTACGGTAAAATACAAGTCGCGTCTACCTACAGAAACAAAGTATTTTCTCTCGTGACTTTGCCTTTTGAACCATTTGGCCAATGCGGCAAGAACAGAACCATTTTGCATCACGTACCTCTTAAAGGGAGCAAGTCTGTGGTGTTTGGAGAATGGGAGATTTCCCAGTTCAACTCGGAGACTGTTGCTGGCATTTACAGTATTGACGTGAGGCTTTATGTTGGGATAAATGTATTCGGCGGGTACTTCAAGACAAGACATTCCAACCCATCACACAAGATCGACTGCAAGCTGAAAGTTCCTTTGCAGTCTAGTGAAACATCTGCAAATCGCTTCACTTTCAAGACTACCAAGTGCAGAAATGATATATCATTGCAGACCCTGAAGCAGCGGGCTGAATCTGTCCAATTTTTCCTTGGTGACCCGACTGTCCAAGTGTTGCTTGAAGTTCCTCCTCAATTCAGCTTTGACATGGACCTTTTTGTCAGGGCGATTTCTGCCAAAATGCTATTCCCCAAGTCTTACTTGGATCTGCAGGCTAGCCAACATAACACAACCATACGGATAATTCGTATACCTCACAACCAAAGACTGCCGAGTGCAGAAGTGCTACATCACTGCAGAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

277

Amino Acids

31.62

Weight (kDa)

9.65

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018053)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18560
rosa_chinensis RchiOBHm_Chr3g0473111
rosa_laevigata RLG00000024027
rosa_multiflora Rmu_co7972084.1_g000001 Rmu_sc0016115.1_g000005
rosa_rugosa Rorug03G0131800
rosa_samantha Rh3AG183100 Rh3BG209600 Rh3CG206600
rosa_wichuraiana Rw0G008070 Rw0G014700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 629, 683
AccB7I CCANNNNNTGG 1 cut(s) 54
AccI GTMKAC 3 cut(s) 19, 227, 775
AccII CGCG 1 cut(s) 224
AciI CCGC 3 cut(s) 288, 441, 592
AclWI GGATC 2 cut(s) 71, 738
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 2 cut(s) 12, 83
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 2 cut(s) 318, 446
AfiI CCNNNNNNNGG 2 cut(s) 54, 326
AgsI TTSAA 6 cut(s) 118, 272, 373, 451, 544, 647
AjiI CACGTC 1 cut(s) 409
AjuI GAANNNNNNNTTGG 2 cut(s) 695, 727
AluBI AGCT 2 cut(s) 493, 666
AluI AGCT 2 cut(s) 493, 666
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 2 cut(s) 71, 738
AlwNI CAGNNNCTG 2 cut(s) 583, 828
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 2 cut(s) 194, 589
ApoI RAATTY 2 cut(s) 12, 83
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 4 cut(s) 115, 160, 166, 632
AsuNHI GCTAGC 1 cut(s) 740
AvaII GGWCC 1 cut(s) 676
BalI TGGCCA 1 cut(s) 282
BauI CACGAG 1 cut(s) 254
BbvI GCAGC 2 cut(s) 181, 601
BccI CCATC 2 cut(s) 179, 478
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 3 cut(s) 38, 513, 741
BfmI CTRYAG 3 cut(s) 232, 734, 820
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 3 cut(s) 195, 289, 590
BlsI GCNGC 3 cut(s) 196, 290, 591
Bme18I GGWCC 1 cut(s) 676
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 676
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 2 cut(s) 88, 319
BmtI GCTAGC 1 cut(s) 744
BmuI ACTGGG 1 cut(s) 361
BsaAI YACGTR 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 616
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 326
Bse1I ACTGG 1 cut(s) 367
Bse3DI GCAATG 1 cut(s) 572
BseDI CCNNGG 1 cut(s) 616
BseLI CCNNNNNNNGG 2 cut(s) 54, 326
BseMI GCAATG 1 cut(s) 572
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 1 cut(s) 645
BseXI GCAGC 2 cut(s) 181, 601
BsgI GTGCAG 2 cut(s) 577, 822
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 1 cut(s) 282
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 54, 326
BsmAI GTCTC 1 cut(s) 374
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 1 cut(s) 282
Bsp143I GATC 3 cut(s) 76, 480, 730
BspACI CCGC 3 cut(s) 288, 441, 592
BspANI GGCC 1 cut(s) 282
BspFNI CGCG 1 cut(s) 224
BspMAI CTGCAG 2 cut(s) 738, 824
BspOI GCTAGC 1 cut(s) 744
BspPI GGATC 2 cut(s) 71, 738
BsrDI GCAATG 1 cut(s) 572
BsrI ACTGG 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 616
BssMI GATC 3 cut(s) 76, 480, 730
BssNAI GTATAC 1 cut(s) 776
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 1 cut(s) 616
Bst1107I GTATAC 1 cut(s) 776
Bst2BI CACGAG 1 cut(s) 254
Bst4CI ACNGT 4 cut(s) 209, 385, 401, 631
BstBAI YACGTR 1 cut(s) 316
BstC8I GCNNGC 5 cut(s) 391, 491, 594, 738, 742
BstEII GGTNACC 1 cut(s) 620
BstENI CCTNNNNNAGG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 79, 483, 733
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 3 cut(s) 76, 480, 730
BstMWI GCNNNNNNNGC 2 cut(s) 288, 698
BstPI GGTNACC 1 cut(s) 620
BstSFI CTRYAG 3 cut(s) 232, 734, 820
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 181, 601
BstX2I RGATCY 2 cut(s) 76, 730
BstYI RGATCY 2 cut(s) 76, 730
BstZ17I GTATAC 1 cut(s) 776
BsuRI GGCC 1 cut(s) 282
BtrI CACGTC 1 cut(s) 409
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 1 cut(s) 817
Cac8I GCNNGC 5 cut(s) 391, 491, 594, 738, 742
CaiI CAGNNNCTG 2 cut(s) 583, 828
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 676
CseI GACGC 1 cut(s) 213
Csp6I GTAC 2 cut(s) 317, 445
CviAII CATG 1 cut(s) 673
CviQI GTAC 2 cut(s) 317, 445
DpnI GATC 3 cut(s) 78, 482, 732
DpnII GATC 3 cut(s) 76, 480, 730
DrdI GACNNNNNNGTC 2 cut(s) 629, 683
DseDI GACNNNNNNGTC 2 cut(s) 629, 683
EaeI YGGCCR 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 676
Eco57I CTGAAG 1 cut(s) 605
Eco91I GGTNACC 1 cut(s) 620
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoO65I GGTNACC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 1 cut(s) 676
FaiI YATR 7 cut(s) 200, 420, 569, 674, 750, 761, 776
FalI AAGNNNNNCTT 4 cut(s) 627, 659, 710, 742
FaqI GGGAC 1 cut(s) 54
FatI CATG 1 cut(s) 672
FauI CCCGC 2 cut(s) 434, 585
FblI GTMKAC 3 cut(s) 19, 227, 775
Fnu4HI GCNGC 3 cut(s) 195, 289, 590
Fsp4HI GCNGC 3 cut(s) 195, 289, 590
FspBI CTAG 3 cut(s) 38, 513, 741
GlaI GCGC 1 cut(s) 159
GluI GCNGC 3 cut(s) 195, 289, 590
HaeIII GGCC 1 cut(s) 282
HgaI GACGC 1 cut(s) 213
HhaI GCGC 1 cut(s) 160
Hin1II CATG 1 cut(s) 676
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 1 cut(s) 599
HphI GGTGA 4 cut(s) 115, 160, 166, 632
Hpy166II GTNNAC 3 cut(s) 20, 228, 776
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 3 cut(s) 256, 451, 544
Hpy8I GTNNAC 3 cut(s) 20, 228, 776
HpyCH4III ACNGT 4 cut(s) 209, 385, 401, 631
HpyCH4IV ACGT 2 cut(s) 315, 408
HpyCH4V TGCA 9 cut(s) 310, 489, 508, 527, 558, 577, 736, 803, 822
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 698
HpySE526I ACGT 2 cut(s) 315, 408
Hsp92II CATG 1 cut(s) 676
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 3 cut(s) 76, 480, 730
LmnI GCTCC 1 cut(s) 330
LpnPI CCDG 6 cut(s) 40, 375, 380, 596, 673, 722
Lsp1109I GCAGC 2 cut(s) 181, 601
LweI GCATC 2 cut(s) 88, 319
MaeI CTAG 3 cut(s) 38, 513, 741
MaeII ACGT 2 cut(s) 315, 408
MaeIII GTNAC 2 cut(s) 256, 620
MalI GATC 3 cut(s) 78, 482, 732
MboI GATC 3 cut(s) 76, 480, 730
MflI RGATCY 2 cut(s) 76, 730
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 7 cut(s) 12, 83, 113, 118, 608, 659, 768
MluNI TGGCCA 1 cut(s) 282
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 8 cut(s) 41, 112, 152, 330, 405, 663, 666, 789
Mox20I TGGCCA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 282
MseI TTAA 1 cut(s) 324
Msp20I TGGCCA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 592
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 288, 698
NdeII GATC 3 cut(s) 76, 480, 730
NheI GCTAGC 1 cut(s) 740
NlaIII CATG 1 cut(s) 676
NmeAIII GCCGAG 1 cut(s) 822
NmuCI GTSAC 2 cut(s) 256, 620
PcsI WCGNNNNNNNCGW 1 cut(s) 769
PfeI GAWTC 1 cut(s) 599
PflMI CCANNNNNTGG 1 cut(s) 54
PkrI GCNGC 3 cut(s) 196, 290, 591
Ppu21I YACGTR 1 cut(s) 316
PspEI GGTNACC 1 cut(s) 620
PspPI GGNCC 1 cut(s) 676
PstI CTGCAG 2 cut(s) 738, 824
PstNI CAGNNNCTG 2 cut(s) 583, 828
PsuI RGATCY 2 cut(s) 76, 730
RsaI GTAC 2 cut(s) 318, 446
RsaNI GTAC 2 cut(s) 317, 445
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 3 cut(s) 195, 289, 590
Sau3AI GATC 3 cut(s) 76, 480, 730
Sau96I GGNCC 1 cut(s) 676
SetI ASST 9 cut(s) 156, 233, 318, 322, 411, 495, 668, 681, 781
SfaNI GCATC 2 cut(s) 88, 319
SfcI CTRYAG 3 cut(s) 232, 734, 820
SinI GGWCC 1 cut(s) 676
Sse9I AATT 7 cut(s) 12, 83, 113, 118, 608, 659, 768
SsiI CCGC 3 cut(s) 288, 441, 592
SspMI CTAG 3 cut(s) 38, 513, 741
StyI CCWWGG 1 cut(s) 616
TaaI ACNGT 4 cut(s) 209, 385, 401, 631
TaiI ACGT 2 cut(s) 318, 411
TaqI TCGA 2 cut(s) 144, 483
TasI AATT 7 cut(s) 12, 83, 113, 118, 608, 659, 768
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 1 cut(s) 599
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 824
TseFI GTSAC 2 cut(s) 256, 620
TseI GCWGC 2 cut(s) 194, 589
Tsp45I GTSAC 2 cut(s) 256, 620
TspDTI ATGAA 2 cut(s) 49, 76
TspGWI ACGGA 1 cut(s) 778
TspRI CASTG 1 cut(s) 824
Van91I CCANNNNNTGG 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 676
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 2 cut(s) 12, 83
XcmI CCANNNNNNNNNTGG 2 cut(s) 614, 724
XmiI GTMKAC 3 cut(s) 19, 227, 775
XspI CTAG 3 cut(s) 38, 513, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.