Rw0G008070

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00301
OG0018053
Physical Location & Seq
Forward (+)
12337 .. 13026
690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008070.1

Sequence Viewer

Length: 690 bp
ATGTGGAGAGGAATTCGTCTACAACTCTGCCTCTTACTAGCCTACCCCACACTCTGGTTCATCTTTTGTCCCAAAGATCCCAAATTCATCATCACAGATGCCTCTCTAACCCAATTCAATTTCACCAACCACAACAAGACCCTCGACTACAACCTTGCGCTCAACATCACCATCACCAACCCCAACAGAAAGGCTGCCATATTCTACGGTAAAATACAAGTCGCGTCTACCTACAGAAACAAAGTATTTTCTATCGTGACTTTACCTTTTGAACCATTTGGCCAATGCGGCAAGAACAGAACCATTTCGCATCACATACCTCTTAAAGGGAGCAAGTCTCTGGTGTTTGGAGAACGGGAGATTTCCCAGTTTAACTCGGAGACTGTTGCTGGCGTTTACAGTATTGACGTGAGGCTTTATGTTGGGATAAGAGTATTCGGCGGGTACTTCAAGACAAGACTGTCAAGACATTCCAACCCATCGCACAAGATCGACTGCAAGCTGAAAGTTCCTTTGCAGTCTAGTGAAACATCTGCAAATCGCTTCACTTTCAAGACTACCAAGTGCAGAAATGATATATCACTGCAGACCCTGAAGCAGCGGGCTGAATCTGTCCAGTTTTTCCTTGGTGACTCGACTGTCCAAGTGTTGCTTGTTTGTATCTATATTTTTGCAGCAGTGCGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

229

Amino Acids

26.1

Weight (kDa)

9.9

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018053)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18560
rosa_chinensis RchiOBHm_Chr3g0473111
rosa_laevigata RLG00000024027
rosa_multiflora Rmu_co7972084.1_g000001 Rmu_sc0016115.1_g000005
rosa_rugosa Rorug03G0131800
rosa_samantha Rh3AG183100 Rh3BG209600 Rh3CG206600
rosa_wichuraiana Rw0G008070 Rw0G014700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 460, 638
AccB7I CCANNNNNTGG 1 cut(s) 54
AccI GTMKAC 2 cut(s) 19, 227
AccII CGCG 1 cut(s) 224
AciI CCGC 3 cut(s) 288, 441, 601
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 2 cut(s) 12, 83
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 2 cut(s) 54, 326
AgsI TTSAA 4 cut(s) 118, 272, 451, 553
AjiI CACGTC 1 cut(s) 409
AluBI AGCT 1 cut(s) 502
AluI AGCT 1 cut(s) 502
Alw26I GTCTC 2 cut(s) 342, 374
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 592
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 3 cut(s) 194, 598, 674
ApoI RAATTY 2 cut(s) 12, 83
Asp700I GAANNNNTTC 1 cut(s) 304
AspLEI GCGC 1 cut(s) 160
AsuHPI GGTGA 4 cut(s) 115, 160, 166, 641
BalI TGGCCA 1 cut(s) 282
BbvI GCAGC 3 cut(s) 181, 610, 686
BccI CCATC 2 cut(s) 179, 487
BcoDI GTCTC 2 cut(s) 342, 374
BfaI CTAG 2 cut(s) 38, 522
BfmI CTRYAG 2 cut(s) 232, 584
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 4 cut(s) 195, 289, 599, 675
BlsI GCNGC 4 cut(s) 196, 290, 600, 676
BmgBI CACGTC 1 cut(s) 409
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 2 cut(s) 88, 319
BmuI ACTGGG 1 cut(s) 361
BplI GAGNNNNNCTC 2 cut(s) 322, 354
BsaJI CCNNGG 1 cut(s) 625
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 326
Bse1I ACTGG 2 cut(s) 367, 616
BseDI CCNNGG 1 cut(s) 625
BseLI CCNNNNNNNGG 2 cut(s) 54, 326
BseNI ACTGG 2 cut(s) 367, 616
BseXI GCAGC 3 cut(s) 181, 610, 686
BsgI GTGCAG 1 cut(s) 586
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 1 cut(s) 282
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 54, 326
BsmAI GTCTC 2 cut(s) 342, 374
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 1 cut(s) 282
Bsp143I GATC 2 cut(s) 76, 489
BspACI CCGC 3 cut(s) 288, 441, 601
BspANI GGCC 1 cut(s) 282
BspFNI CGCG 1 cut(s) 224
BspMAI CTGCAG 1 cut(s) 588
BspPI GGATC 1 cut(s) 71
BsrI ACTGG 2 cut(s) 367, 616
BssECI CCNNGG 1 cut(s) 625
BssMI GATC 2 cut(s) 76, 489
BssT1I CCWWGG 1 cut(s) 625
Bst4CI ACNGT 5 cut(s) 209, 385, 401, 462, 640
BstC8I GCNNGC 3 cut(s) 391, 500, 603
BstENI CCTNNNNNAGG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 2 cut(s) 79, 492
BstMAI GTCTC 2 cut(s) 342, 374
BstMBI GATC 2 cut(s) 76, 489
BstMWI GCNNNNNNNGC 1 cut(s) 288
BstSFI CTRYAG 2 cut(s) 232, 584
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 3 cut(s) 181, 610, 686
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 465
BtrI CACGTC 1 cut(s) 409
BtsI GCAGTG 2 cut(s) 581, 684
BtsIMutI CAGTG 2 cut(s) 581, 684
Cac8I GCNNGC 3 cut(s) 391, 500, 603
CaiI CAGNNNCTG 1 cut(s) 592
CfoI GCGC 1 cut(s) 160
CseI GACGC 1 cut(s) 213
Csp6I GTAC 1 cut(s) 445
CviJI RGCY 6 cut(s) 41, 194, 282, 415, 502, 605
CviKI_1 RGCY 6 cut(s) 41, 194, 282, 415, 502, 605
CviQI GTAC 1 cut(s) 445
DpnI GATC 2 cut(s) 78, 491
DpnII GATC 2 cut(s) 76, 489
DrdI GACNNNNNNGTC 2 cut(s) 460, 638
DseDI GACNNNNNNGTC 2 cut(s) 460, 638
EaeI YGGCCR 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 614
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 625
FaiI YATR 5 cut(s) 200, 317, 420, 578, 666
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 1 cut(s) 54
FauI CCCGC 2 cut(s) 434, 594
FblI GTMKAC 2 cut(s) 19, 227
Fnu4HI GCNGC 4 cut(s) 195, 289, 599, 675
Fsp4HI GCNGC 4 cut(s) 195, 289, 599, 675
FspBI CTAG 2 cut(s) 38, 522
GlaI GCGC 1 cut(s) 159
GluI GCNGC 4 cut(s) 195, 289, 599, 675
HaeIII GGCC 1 cut(s) 282
HgaI GACGC 1 cut(s) 213
HhaI GCGC 1 cut(s) 160
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 2 cut(s) 608, 632
HphI GGTGA 4 cut(s) 115, 160, 166, 641
Hpy166II GTNNAC 3 cut(s) 20, 228, 397
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 4 cut(s) 256, 451, 465, 553
Hpy8I GTNNAC 3 cut(s) 20, 228, 397
HpyCH4III ACNGT 5 cut(s) 209, 385, 401, 462, 640
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 6 cut(s) 498, 517, 536, 567, 586, 674
HpyF10VI GCNNNNNNNGC 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 408
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 2 cut(s) 76, 489
LmnI GCTCC 1 cut(s) 330
LpnPI CCDG 6 cut(s) 40, 326, 375, 380, 605, 629
Lsp1109I GCAGC 3 cut(s) 181, 610, 686
LweI GCATC 2 cut(s) 88, 319
MaeI CTAG 2 cut(s) 38, 522
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 256, 629
MalI GATC 2 cut(s) 78, 491
MboI GATC 2 cut(s) 76, 489
MflI RGATCY 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 4 cut(s) 12, 83, 113, 118
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 5 cut(s) 41, 112, 152, 330, 405
Mox20I TGGCCA 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 304
MscI TGGCCA 1 cut(s) 282
MseI TTAA 3 cut(s) 324, 372, 688
Msp20I TGGCCA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 601
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 288
NdeII GATC 2 cut(s) 76, 489
NmuCI GTSAC 2 cut(s) 256, 629
PdmI GAANNNNTTC 1 cut(s) 304
PfeI GAWTC 1 cut(s) 608
PflMI CCANNNNNTGG 1 cut(s) 54
PkrI GCNGC 4 cut(s) 196, 290, 600, 676
PleI GAGTC 1 cut(s) 626
PpsI GAGTC 1 cut(s) 626
PstI CTGCAG 1 cut(s) 588
PstNI CAGNNNCTG 1 cut(s) 592
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SaqAI TTAA 3 cut(s) 324, 372, 688
SatI GCNGC 4 cut(s) 195, 289, 599, 675
Sau3AI GATC 2 cut(s) 76, 489
SchI GAGTC 1 cut(s) 626
SetI ASST 6 cut(s) 156, 233, 268, 322, 411, 504
SfaNI GCATC 2 cut(s) 88, 319
SfcI CTRYAG 2 cut(s) 232, 584
Sse9I AATT 4 cut(s) 12, 83, 113, 118
SsiI CCGC 3 cut(s) 288, 441, 601
SspMI CTAG 2 cut(s) 38, 522
StyI CCWWGG 1 cut(s) 625
TaaI ACNGT 5 cut(s) 209, 385, 401, 462, 640
TaiI ACGT 1 cut(s) 411
TaqI TCGA 3 cut(s) 144, 492, 635
TasI AATT 4 cut(s) 12, 83, 113, 118
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 3 cut(s) 324, 372, 688
Tru9I TTAA 3 cut(s) 324, 372, 688
TscAI CASTG 2 cut(s) 588, 684
TseFI GTSAC 2 cut(s) 256, 629
TseI GCWGC 3 cut(s) 194, 598, 674
Tsp45I GTSAC 2 cut(s) 256, 629
TspDTI ATGAA 2 cut(s) 49, 76
TspRI CASTG 2 cut(s) 588, 684
Van91I CCANNNNNTGG 1 cut(s) 54
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 2 cut(s) 12, 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XmiI GTMKAC 2 cut(s) 19, 227
XmnI GAANNNNTTC 1 cut(s) 304
XspI CTAG 2 cut(s) 38, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.