RLG00000025653

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0019717
Physical Location & Seq
Forward (+)
47380048 .. 47380616
569 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025653

Sequence Viewer

Length: 471 bp
ATGGTGACTACTAGAGGAATGAACAAGAAGGCACCAGAATCTTTGAAAGCCACCACCACCATTCGGCCAGCAAAGCAGGGCAAGAAGGCTCCCCAAGCTAACAAAAACATATCATGTGAGCTAGAGCTGAAGACGAATGTATGTGTCATTCACTGTTATGAATCTCGCGATGATGTTACTATATGTGATGGGGATGAGGGACTTCATGAGTGTGGTGTTCAAAACACACCTGCTGCTGCTGCTGATGCAGGAGGAGCATCATCAAGCCGCAGCAGGCTTGACACCTCATCATTGAAGCAGAGGGCTGAAGCGATAGAAGCGCTGCTTGAGCTTTGTGCAAGATTGTTCCAAGATGGAAAATATCAAGAGCTTGAGGTTGTGCTTAAGCCTTTTGGGCCTGGGGATGTTTGCCCTAGGGAAACTGCTATTTGGTTGACTAAGATCATTAAGGAAAATACTACTGTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

157

Amino Acids

16.93

Weight (kDa)

7.54

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03290
rosa_laevigata RLG00000025653
rosa_multiflora Rmu_sc0004924.1_g000002 Rmu_sc0009360.1_g000002
rosa_samantha Rh3BG038600 Rh3CG037100 Rh3DG037800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 238
Acc36I ACCTGC 1 cut(s) 238
AccB1I GGYRCC 1 cut(s) 31
AccII CGCG 1 cut(s) 168
AciI CCGC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 65
AcuI CTGAAG 2 cut(s) 149, 327
AfeI AGCGCT 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 63
AflII CTTAAG 1 cut(s) 383
AgsI TTSAA 3 cut(s) 46, 221, 295
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 5 cut(s) 98, 121, 127, 331, 370
AluI AGCT 5 cut(s) 98, 121, 127, 331, 370
Aor51HI AGCGCT 1 cut(s) 321
AoxI GGCC 2 cut(s) 65, 395
ApeKI GCWGC 5 cut(s) 233, 236, 239, 270, 322
AspA2I CCTAGG 1 cut(s) 413
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 16
AvrII CCTAGG 1 cut(s) 413
BanI GGYRCC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 137
BbvI GCAGC 5 cut(s) 220, 223, 226, 282, 309
BccI CCATC 2 cut(s) 182, 347
BciT130I CCWGG 1 cut(s) 399
BfaI CTAG 3 cut(s) 12, 122, 414
BfoI RGCGCY 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 238
BglI GCCNNNNNGGC 1 cut(s) 394
BisI GCNGC 6 cut(s) 234, 237, 240, 268, 271, 323
BlnI CCTAGG 1 cut(s) 413
BlsI GCNGC 6 cut(s) 235, 238, 241, 269, 272, 324
Bme1390I CCNGG 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 2 cut(s) 33, 90
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 2 cut(s) 235, 266
BpiI GAAGAC 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 347, 392
BsaJI CCNNGG 2 cut(s) 398, 413
Bsc4I CCNNNNNNNGG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 398, 413
BseGI GGATG 2 cut(s) 199, 409
BseLI CCNNNNNNNGG 1 cut(s) 63
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 5 cut(s) 220, 223, 226, 282, 309
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 67, 397
BshNI GGYRCC 1 cut(s) 31
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 2 cut(s) 67, 397
Bsp143I GATC 1 cut(s) 441
Bsp68I TCGCGA 1 cut(s) 168
BspACI CCGC 1 cut(s) 268
BspANI GGCC 2 cut(s) 67, 397
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 33, 90
BspMI ACCTGC 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 31
BspTI CTTAAG 1 cut(s) 383
BssECI CCNNGG 2 cut(s) 398, 413
BssMI GATC 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 413
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 155, 463
BstAFI CTTAAG 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 69, 275
BstDEI CTNAG 1 cut(s) 438
BstF5I GGATG 2 cut(s) 199, 409
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 1 cut(s) 444
BstMBI GATC 1 cut(s) 441
BstMWI GCNNNNNNNGC 8 cut(s) 73, 95, 239, 245, 254, 317, 328, 394
BstNI CCWGG 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 397
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 5 cut(s) 220, 223, 226, 282, 309
BstV2I GAAGAC 1 cut(s) 137
BsuRI GGCC 2 cut(s) 67, 397
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 199, 409
BtsIMutI CAGTG 1 cut(s) 151
BtuMI TCGCGA 1 cut(s) 168
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 2 cut(s) 69, 275
CciI TCATGA 1 cut(s) 205
CfoI GCGC 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 395
CviAII CATG 2 cut(s) 114, 206
DdeI CTNAG 1 cut(s) 438
DpnI GATC 1 cut(s) 443
DpnII GATC 1 cut(s) 441
EaeI YGGCCR 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 413
Eco47III AGCGCT 1 cut(s) 321
Eco57I CTGAAG 2 cut(s) 149, 327
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 2 cut(s) 117, 209
FaiI YATR 7 cut(s) 110, 115, 142, 159, 182, 184, 207
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 213
FatI CATG 2 cut(s) 113, 205
Fnu4HI GCNGC 6 cut(s) 234, 237, 240, 268, 271, 323
FokI GGATG 2 cut(s) 206, 416
Fsp4HI GCNGC 6 cut(s) 234, 237, 240, 268, 271, 323
FspBI CTAG 3 cut(s) 12, 122, 414
GlaI GCGC 1 cut(s) 321
GluI GCNGC 6 cut(s) 234, 237, 240, 268, 271, 323
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 2 cut(s) 67, 397
HhaI GCGC 1 cut(s) 322
Hin1II CATG 2 cut(s) 117, 209
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HinfI GANTC 2 cut(s) 38, 161
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 435
Hpy188III TCNNGA 3 cut(s) 167, 206, 365
Hpy8I GTNNAC 1 cut(s) 435
HpyAV CCTTC 2 cut(s) 22, 79
HpyCH4III ACNGT 2 cut(s) 155, 463
HpyCH4V TGCA 2 cut(s) 248, 338
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 95, 239, 245, 254, 317, 328, 394
HpyF3I CTNAG 1 cut(s) 438
Hsp92II CATG 2 cut(s) 117, 209
HspAI GCGC 1 cut(s) 320
Kzo9I GATC 1 cut(s) 441
LmnI GCTCC 2 cut(s) 94, 254
LpnPI CCDG 8 cut(s) 48, 62, 81, 234, 243, 259, 384, 411
Lsp1109I GCAGC 5 cut(s) 220, 223, 226, 282, 309
LweI GCATC 2 cut(s) 235, 266
MaeI CTAG 3 cut(s) 12, 122, 414
MaeIII GTNAC 2 cut(s) 4, 175
MalI GATC 1 cut(s) 443
MboI GATC 1 cut(s) 441
MboII GAAGA 1 cut(s) 142
MnlI CCTC 6 cut(s) 8, 190, 245, 294, 295, 367
MseI TTAA 2 cut(s) 384, 447
MslI CAYNNNNRTG 2 cut(s) 156, 210
MspCI CTTAAG 1 cut(s) 383
MspR9I CCNGG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 8 cut(s) 73, 95, 239, 245, 254, 317, 328, 394
NdeII GATC 1 cut(s) 441
NlaIII CATG 2 cut(s) 117, 209
NlaIV GGNNCC 2 cut(s) 33, 90
NmuCI GTSAC 1 cut(s) 4
NruI TCGCGA 1 cut(s) 168
PagI TCATGA 1 cut(s) 205
PaqCI CACCTGC 1 cut(s) 238
PfeI GAWTC 2 cut(s) 38, 161
PkrI GCNGC 6 cut(s) 235, 238, 241, 269, 272, 324
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 2 cut(s) 33, 90
PspPI GGNCC 1 cut(s) 395
RruI TCGCGA 1 cut(s) 168
RseI CAYNNNNRTG 2 cut(s) 156, 210
SaqAI TTAA 2 cut(s) 384, 447
SatI GCNGC 6 cut(s) 234, 237, 240, 268, 271, 323
Sau3AI GATC 1 cut(s) 441
Sau96I GGNCC 1 cut(s) 395
ScrFI CCNGG 1 cut(s) 399
SetI ASST 8 cut(s) 100, 123, 129, 232, 287, 333, 372, 378
SfaNI GCATC 2 cut(s) 235, 266
SmiMI CAYNNNNRTG 2 cut(s) 156, 210
SmlI CTYRAG 3 cut(s) 326, 371, 383
SmoI CTYRAG 3 cut(s) 326, 371, 383
SsiI CCGC 1 cut(s) 268
SspMI CTAG 3 cut(s) 12, 122, 414
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 2 cut(s) 155, 463
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 2 cut(s) 38, 161
Tru1I TTAA 2 cut(s) 384, 447
Tru9I TTAA 2 cut(s) 384, 447
TscAI CASTG 1 cut(s) 158
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 5 cut(s) 233, 236, 239, 270, 322
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 35, 174, 194
TspRI CASTG 1 cut(s) 158
Vha464I CTTAAG 1 cut(s) 383
XmaJI CCTAGG 1 cut(s) 413
XspI CTAG 3 cut(s) 12, 122, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.