RLG00000025926

At4g13230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017676
Physical Location & Seq
Reverse (-)
49262747 .. 49263373
627 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025926

Sequence Viewer

Length: 369 bp
ATGGCAAGCCTCAGCACCAAACTTGGAGCAATTGCAAGGACTCCAACTTCTAGGATTTTTTCGAGACGGATTCAAGCGGGTTCGCAGCAAGGAAGAGACGCACTACAACAAGGAGCAAATGAAGCTAGGAATACTGGTGAACAGGTGGCAAATCAGAGCAATGATATGGCTGGGAAGGTGTCTGCAACAGCACAAGATATGACTGAGAAAGCAAAGCAGACAGTGCAGGATGCATGGGGCTCAGCTAAGGACACTGCCCAGAAGGCGGCCGACAACGTCATGGGAAAGGCACAAGAATCAAAGGAGTTCGTCAAAGAAAATGCAGAGCAGGTCAAGAAAAGCATGAACACCAAGGGCCCTATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

13.03

Weight (kDa)

9.69

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017676)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g00850
rosa_chinensis RchiOBHm_Chr3g0448341
rosa_laevigata RLG00000025926
rosa_multiflora Rmu_sc0005582.1_g000008 Rmu_sc0015167.1_g000006
rosa_roxburghii Rroxscaffold_6G00426470
rosa_rugosa Rorug02G0610700
rosa_samantha Rh3AG010200 Rh3BG009800 Rh3CG009700 Rh3DG010300
rosa_wichuraiana Rw3G000820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 319
AciI CCGC 2 cut(s) 77, 266
AcoI YGGCCR 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 1 cut(s) 74
AluBI AGCT 2 cut(s) 125, 245
AluI AGCT 2 cut(s) 125, 245
Alw26I GTCTC 2 cut(s) 58, 90
AoxI GGCC 2 cut(s) 267, 355
ApaI GGGCCC 1 cut(s) 359
ApeKI GCWGC 1 cut(s) 85
AspS9I GGNCC 2 cut(s) 355, 356
AsuHPI GGTGA 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 359
BanII GRGCYC 2 cut(s) 242, 359
BbvCI CCTCAGC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 58, 90
BfaI CTAG 2 cut(s) 51, 126
BfuAI ACCTGC 1 cut(s) 319
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 2 cut(s) 86, 267
BlpI GCTNAGC 1 cut(s) 241
BlsI GCNGC 2 cut(s) 87, 268
BmgT120I GGNCC 2 cut(s) 355, 356
BmiI GGNNCC 1 cut(s) 357
BmsI GCATC 1 cut(s) 220
Bpu10I CCTNAGC 2 cut(s) 11, 246
Bpu1102I GCTNAGC 1 cut(s) 241
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 3 cut(s) 25, 195, 255
BseNI ACTGG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 359
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 97
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 245
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 2 cut(s) 269, 357
BsiEI CGRYCG 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 2 cut(s) 58, 90
BsmBI CGTCTC 2 cut(s) 58, 90
BsnI GGCC 2 cut(s) 269, 357
Bsp120I GGGCCC 1 cut(s) 355
Bsp1286I GDGCHC 2 cut(s) 242, 359
Bsp1720I GCTNAGC 1 cut(s) 241
BspACI CCGC 2 cut(s) 77, 266
BspANI GGCC 2 cut(s) 269, 357
BspCNI CTCAG 3 cut(s) 24, 196, 254
BspLI GGNNCC 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 166
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 4 cut(s) 11, 204, 241, 246
BstF5I GGATG 1 cut(s) 235
BstMAI GTCTC 2 cut(s) 58, 90
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 3 cut(s) 122, 223, 263
BstSLI GKGCMC 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 97
BstZI CGGCCG 1 cut(s) 267
BsuRI GGCC 2 cut(s) 269, 357
BtsCI GGATG 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 228, 252
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 355, 356
CseI GACGC 1 cut(s) 107
CviAII CATG 3 cut(s) 234, 280, 343
CviJI RGCY 7 cut(s) 9, 125, 170, 240, 245, 269, 357
CviKI_1 RGCY 7 cut(s) 9, 125, 170, 240, 245, 269, 357
DdeI CTNAG 4 cut(s) 11, 204, 241, 246
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
EclXI CGGCCG 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 242, 359
Eco52I CGGCCG 1 cut(s) 267
EcoO109I RGGNCCY 2 cut(s) 355, 356
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 2 cut(s) 242, 359
ErhI CCWWGG 1 cut(s) 351
Esp3I CGTCTC 2 cut(s) 58, 90
FaeI CATG 3 cut(s) 237, 283, 346
FaiI YATR 5 cut(s) 167, 200, 235, 281, 344
FatI CATG 3 cut(s) 233, 279, 342
FauI CCCGC 1 cut(s) 70
Fnu4HI GCNGC 2 cut(s) 86, 267
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 2 cut(s) 242, 359
Fsp4HI GCNGC 2 cut(s) 86, 267
FspBI CTAG 2 cut(s) 51, 126
GluI GCNGC 2 cut(s) 86, 267
GsaI CCCAGC 1 cut(s) 174
HaeIII GGCC 2 cut(s) 269, 357
HgaI GACGC 1 cut(s) 107
Hin1II CATG 3 cut(s) 237, 283, 346
HinfI GANTC 3 cut(s) 40, 70, 296
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 63, 334
Hpy8I GTNNAC 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 169, 256
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 5 cut(s) 35, 185, 226, 233, 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 223, 263
HpyF3I CTNAG 4 cut(s) 11, 204, 241, 246
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 3 cut(s) 237, 283, 346
LmnI GCTCC 2 cut(s) 26, 113
LpnPI CCDG 6 cut(s) 120, 128, 156, 212, 272, 314
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 51, 126
MaeII ACGT 1 cut(s) 276
MboII GAAGA 1 cut(s) 105
MfeI CAATTG 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 242, 359
MluCI AATT 2 cut(s) 30, 364
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 68
MnlI CCTC 1 cut(s) 20
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 1 cut(s) 367
MunI CAATTG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 122, 223, 263
NlaIII CATG 3 cut(s) 237, 283, 346
NlaIV GGNNCC 1 cut(s) 357
NsiI ATGCAT 1 cut(s) 235
PfeI GAWTC 2 cut(s) 70, 296
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 2 cut(s) 87, 268
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 357
PspOMI GGGCCC 1 cut(s) 355
PspPI GGNCC 2 cut(s) 355, 356
PsyI GACNNNGTC 1 cut(s) 275
SaqAI TTAA 1 cut(s) 367
SatI GCNGC 2 cut(s) 86, 267
Sau96I GGNCC 2 cut(s) 355, 356
SchI GAGTC 1 cut(s) 34
SduI GDGCHC 2 cut(s) 242, 359
SetI ASST 6 cut(s) 127, 147, 180, 247, 279, 333
SfaNI GCATC 1 cut(s) 220
Sse9I AATT 2 cut(s) 30, 364
SsiI CCGC 2 cut(s) 77, 266
SspMI CTAG 2 cut(s) 51, 126
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 62
TasI AATT 2 cut(s) 30, 364
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 2 cut(s) 70, 296
Tru1I TTAA 1 cut(s) 367
Tru9I TTAA 1 cut(s) 367
TscAI CASTG 2 cut(s) 228, 259
TseI GCWGC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 135, 359
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 2 cut(s) 228, 259
Tth111I GACNNNGTC 1 cut(s) 275
XspI CTAG 2 cut(s) 51, 126
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.