Rorug02G0610700

At4g13230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017676
Physical Location & Seq
Reverse (-)
72721928 .. 72723689
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0610700.1

Sequence Viewer

Length: 1059 bp
ATGGCGCCTTGTACATGGGGTTATGTGTTGCCTTATGCAGCTATGGTAATGGTGCAGTTCATAGATATAGGTGCAGCAACTATAAACAAAGCAGCCATGTCGAAAGGAATGAGCAGTTATGTTTTAGTTGTCTACTCTAGTGCGCTTGCTACCATTTTTCTCCTTCCTTGTTTCATCTTACAAAAGAAGATTTCACTTACTTTTTCATTCCTTGGTGGACAATTCCTTCTTGGCCTAATTGGGAGTTCAAATATGTTATTGGCATATACTGGCATCAACTACAGCTCACCAACACTTGCTTCAGCTATGGGAAACGTCATTCCAATCTTTACTTTCATACTGGCAATCATTTTCAGGATGGAAAAGCTGGATTTGAGGAGGTCAAGCTGTCAAGCCAAAGTGTTAGGGACTATTGTATCAGTATCTGGAGCATTTATAGTAATTCTATACAAGGGATCAGCAATCTTTTCACCATCCAACTCTCCTGACCAGAACTTCATGATGACCTTGCAGCAGTCAAAGTGGGTCTTTGGAGGTCTTATGCTTGCAATGGCGAGCGTTTTGAAGGCATCATGGAATATTCTTCAGACATCAATTGTTAAGAACTGTCCATCAATGATTACCGTACTCTTCTTCTACACCTTCTTTGTGACAATTCAATCCACAGTGTTCTCTCTATTTTTGGAAGGGAATCCAAATGCTTGGGTACTTAGGCCTGACATTGAGATGATCTCCATTGTCTTCTCAGCTATCTTCGGGAGTCTGTTCCATATTGGTGTTGAAACCTGGTGCTTGCACCAAAAGGGACCTATCTTTGTAGCCATGTTCAGGCCCTTGGGAGTTGCCATTGCTGCAGTCATGGTAGTTATCTTCCTTGGCGACGCACTTCACCTTGGCAGTGTGATTGGATCTATCATTATTGCAATTGGATTTTATGCTATGATGTGGGCACAAGTCAAAGAAAGTAGCACCAGTGTTATAGATAATGAAGTTCCAAGCTTGGCATCCACTCAAAAGACCCCTCTTCTGCAATGCAGAACCTCAGTAGAAGATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

352

Amino Acids

38.31

Weight (kDa)

8.86

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 27 - 148 3.8e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017676)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g00850
rosa_chinensis RchiOBHm_Chr3g0448341
rosa_laevigata RLG00000025926
rosa_multiflora Rmu_sc0005582.1_g000008 Rmu_sc0015167.1_g000006
rosa_roxburghii Rroxscaffold_6G00426470
rosa_rugosa Rorug02G0610700
rosa_samantha Rh3AG010200 Rh3BG009800 Rh3CG009700 Rh3DG010300
rosa_wichuraiana Rw3G000820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 132
AclWI GGATC 2 cut(s) 463, 916
AcuI CTGAAG 2 cut(s) 285, 569
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 13, 627, 708
AfiI CCNNNNNNNGG 1 cut(s) 828
AgsI TTSAA 4 cut(s) 249, 565, 659, 782
AjnI CCWGG 1 cut(s) 785
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 7 cut(s) 41, 285, 305, 367, 387, 749, 999
AluI AGCT 7 cut(s) 41, 285, 305, 367, 387, 749, 999
AlwI GGATC 2 cut(s) 463, 916
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 3 cut(s) 232, 713, 830
ApeKI GCWGC 5 cut(s) 38, 74, 92, 511, 851
AspLEI GCGC 2 cut(s) 7, 145
AspS9I GGNCC 2 cut(s) 806, 831
AsuHPI GGTGA 3 cut(s) 279, 462, 881
AvaII GGWCC 1 cut(s) 806
BaeGI GKGCMC 1 cut(s) 952
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 733
BbvI GCAGC 5 cut(s) 50, 86, 104, 523, 838
BccI CCATC 3 cut(s) 352, 481, 619
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BciT130I CCWGG 1 cut(s) 787
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 2 cut(s) 280, 852
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 5 cut(s) 39, 75, 93, 512, 852
BlsI GCNGC 5 cut(s) 40, 76, 94, 513, 853
Bme1390I CCNGG 1 cut(s) 787
Bme18I GGWCC 1 cut(s) 806
BmgT120I GGNCC 2 cut(s) 806, 831
BmiI GGNNCC 2 cut(s) 6, 807
BmrFI CCNGG 1 cut(s) 787
BmsI GCATC 3 cut(s) 282, 578, 1013
BpiI GAAGAC 1 cut(s) 733
BplI GAGNNNNNCTC 2 cut(s) 716, 748
BpmI CTGGAG 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 211, 834, 874, 892
Bsc4I CCNNNNNNNGG 1 cut(s) 828
Bse1I ACTGG 3 cut(s) 274, 345, 972
Bse3DI GCAATG 3 cut(s) 555, 846, 1037
BseBI CCWGG 1 cut(s) 787
BseDI CCNNGG 4 cut(s) 211, 834, 874, 892
BseGI GGATG 3 cut(s) 363, 473, 1004
BseLI CCNNNNNNNGG 1 cut(s) 828
BseMI GCAATG 3 cut(s) 555, 846, 1037
BseMII CTCAG 2 cut(s) 759, 1056
BseNI ACTGG 3 cut(s) 274, 345, 972
BseRI GAGGAG 1 cut(s) 391
BseSI GKGCMC 1 cut(s) 952
BseXI GCAGC 5 cut(s) 50, 86, 104, 523, 838
BsgI GTGCAG 2 cut(s) 74, 93
BshFI GGCC 3 cut(s) 234, 715, 832
BshNI GGYRCC 1 cut(s) 4
BslFI GGGAC 2 cut(s) 421, 819
BslI CCNNNNNNNGG 1 cut(s) 828
BsmFI GGGAC 2 cut(s) 421, 819
BsnI GGCC 3 cut(s) 234, 715, 832
Bsp1286I GDGCHC 1 cut(s) 952
Bsp1407I TGTACA 1 cut(s) 11
Bsp143I GATC 3 cut(s) 455, 729, 908
BspANI GGCC 3 cut(s) 234, 715, 832
BspCNI CTCAG 2 cut(s) 758, 1055
BspHI TCATGA 1 cut(s) 498
BspLI GGNNCC 2 cut(s) 6, 807
BspMAI CTGCAG 1 cut(s) 856
BspPI GGATC 2 cut(s) 463, 916
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 3 cut(s) 555, 846, 1037
BsrGI TGTACA 1 cut(s) 11
BsrI ACTGG 3 cut(s) 274, 345, 972
BssECI CCNNGG 4 cut(s) 211, 834, 874, 892
BssMI GATC 3 cut(s) 455, 729, 908
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 4 cut(s) 211, 834, 874, 892
Bst2UI CCWGG 1 cut(s) 787
Bst4CI ACNGT 3 cut(s) 608, 625, 667
Bst6I CTCTTC 2 cut(s) 635, 1029
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 11
BstC8I GCNNGC 4 cut(s) 147, 546, 556, 794
BstDEI CTNAG 3 cut(s) 710, 745, 1042
BstF5I GGATG 3 cut(s) 363, 473, 1004
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 145
BstKTI GATC 3 cut(s) 458, 732, 911
BstMBI GATC 3 cut(s) 455, 729, 908
BstMWI GCNNNNNNNGC 1 cut(s) 851
BstNI CCWGG 1 cut(s) 787
BstSCI CCNGG 1 cut(s) 785
BstSFI CTRYAG 2 cut(s) 280, 852
BstSLI GKGCMC 1 cut(s) 952
BstV1I GCAGC 5 cut(s) 50, 86, 104, 523, 838
BstV2I GAAGAC 1 cut(s) 733
BstX2I RGATCY 1 cut(s) 908
BstXI CCANNNNNNTGG 1 cut(s) 702
BstYI RGATCY 1 cut(s) 908
BsuRI GGCC 3 cut(s) 234, 715, 832
BtsCI GGATG 3 cut(s) 363, 473, 1004
BtsI GCAGTG 1 cut(s) 904
BtsIMutI CAGTG 3 cut(s) 672, 904, 979
Cac8I GCNNGC 4 cut(s) 147, 546, 556, 794
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 498
CfoI GCGC 2 cut(s) 7, 145
Cfr13I GGNCC 2 cut(s) 806, 831
CseI GACGC 1 cut(s) 890
CsiI ACCWGGT 1 cut(s) 785
Csp6I GTAC 3 cut(s) 12, 626, 707
CviAII CATG 6 cut(s) 15, 97, 499, 573, 823, 859
CviQI GTAC 3 cut(s) 12, 626, 707
DdeI CTNAG 3 cut(s) 710, 745, 1042
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 457, 731, 910
DpnII GATC 3 cut(s) 455, 729, 908
Eam1104I CTCTTC 2 cut(s) 635, 1029
EarI CTCTTC 2 cut(s) 635, 1029
Eco130I CCWWGG 4 cut(s) 211, 834, 874, 892
Eco147I AGGCCT 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 806
Eco57I CTGAAG 2 cut(s) 285, 569
EcoO109I RGGNCCY 2 cut(s) 806, 831
EcoRII CCWGG 1 cut(s) 785
EcoT14I CCWWGG 4 cut(s) 211, 834, 874, 892
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 211, 834, 874, 892
FaeI CATG 6 cut(s) 18, 100, 502, 576, 826, 862
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 2 cut(s) 421, 819
FatI CATG 6 cut(s) 14, 96, 498, 572, 822, 858
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 5 cut(s) 39, 75, 93, 512, 852
FokI GGATG 3 cut(s) 370, 460, 991
Fsp4HI GCNGC 5 cut(s) 39, 75, 93, 512, 852
FspBI CTAG 1 cut(s) 138
GlaI GCGC 2 cut(s) 6, 144
GluI GCNGC 5 cut(s) 39, 75, 93, 512, 852
GsuI CTGGAG 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 234, 715, 832
HgaI GACGC 1 cut(s) 890
HhaI GCGC 2 cut(s) 7, 145
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 18, 100, 502, 576, 826, 862
Hin6I GCGC 2 cut(s) 5, 143
HinP1I GCGC 2 cut(s) 5, 143
HindIII AAGCTT 1 cut(s) 997
HinfI GANTC 2 cut(s) 691, 760
HphI GGTGA 3 cut(s) 279, 462, 881
Hpy166II GTNNAC 2 cut(s) 133, 218
Hpy188I TCNGA 1 cut(s) 588
Hpy188III TCNNGA 5 cut(s) 355, 426, 485, 499, 757
Hpy8I GTNNAC 2 cut(s) 133, 218
Hpy99I CGWCG 1 cut(s) 884
HpyAV CCTTC 5 cut(s) 173, 236, 559, 652, 680
HpyCH4III ACNGT 3 cut(s) 608, 625, 667
HpyCH4IV ACGT 1 cut(s) 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 851
HpyF3I CTNAG 3 cut(s) 710, 745, 1042
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 18, 100, 502, 576, 826, 862
HspAI GCGC 2 cut(s) 5, 143
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 3 cut(s) 455, 729, 908
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 5 cut(s) 50, 86, 104, 523, 838
LweI GCATC 3 cut(s) 282, 578, 1013
MabI ACCWGGT 1 cut(s) 785
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 649
MalI GATC 3 cut(s) 457, 731, 910
MboI GATC 3 cut(s) 455, 729, 908
MboII GAAGA 8 cut(s) 199, 575, 622, 625, 733, 745, 862, 1016
MfeI CAATTG 2 cut(s) 594, 924
MflI RGATCY 1 cut(s) 908
MhlI GDGCHC 1 cut(s) 952
MluCI AATT 6 cut(s) 221, 237, 441, 594, 654, 924
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 769
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 5 cut(s) 369, 372, 527, 1032, 1051
MseI TTAA 1 cut(s) 600
MslI CAYNNNNRTG 2 cut(s) 725, 774
MspR9I CCNGG 1 cut(s) 787
MunI CAATTG 2 cut(s) 594, 924
MvaI CCWGG 1 cut(s) 787
MwoI GCNNNNNNNGC 1 cut(s) 851
NarI GGCGCC 1 cut(s) 5
NdeII GATC 3 cut(s) 455, 729, 908
NlaIII CATG 6 cut(s) 18, 100, 502, 576, 826, 862
NlaIV GGNNCC 2 cut(s) 6, 807
NmuCI GTSAC 1 cut(s) 649
PagI TCATGA 1 cut(s) 498
PceI AGGCCT 1 cut(s) 715
PfeI GAWTC 1 cut(s) 691
PkrI GCNGC 5 cut(s) 40, 76, 94, 513, 853
PleI GAGTC 1 cut(s) 768
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 768
PpuMI RGGWCCY 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 785
PspGI CCWGG 1 cut(s) 785
PspN4I GGNNCC 2 cut(s) 6, 807
PspPI GGNCC 2 cut(s) 806, 831
PspPPI RGGWCCY 1 cut(s) 806
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 908
RsaI GTAC 3 cut(s) 13, 627, 708
RsaNI GTAC 3 cut(s) 12, 626, 707
RseI CAYNNNNRTG 2 cut(s) 725, 774
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 5 cut(s) 39, 75, 93, 512, 852
Sau3AI GATC 3 cut(s) 455, 729, 908
Sau96I GGNCC 2 cut(s) 806, 831
SchI GAGTC 1 cut(s) 769
ScrFI CCNGG 1 cut(s) 787
SduI GDGCHC 1 cut(s) 952
SexAI ACCWGGT 1 cut(s) 785
SfaNI GCATC 3 cut(s) 282, 578, 1013
SfcI CTRYAG 2 cut(s) 280, 852
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 806
SmiMI CAYNNNNRTG 2 cut(s) 725, 774
Sse9I AATT 6 cut(s) 221, 237, 441, 594, 654, 924
SseBI AGGCCT 1 cut(s) 715
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 138
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 1 cut(s) 785
StyI CCWWGG 4 cut(s) 211, 834, 874, 892
TaaI ACNGT 3 cut(s) 608, 625, 667
TaiI ACGT 1 cut(s) 318
TaqI TCGA 1 cut(s) 101
TasI AATT 6 cut(s) 221, 237, 441, 594, 654, 924
TatI WGTACW 1 cut(s) 11
TfiI GAWTC 1 cut(s) 691
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 3 cut(s) 672, 904, 979
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 5 cut(s) 38, 74, 92, 511, 851
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 6 cut(s) 49, 163, 195, 325, 487, 1002
TspRI CASTG 3 cut(s) 672, 904, 979
VpaK11BI GGWCC 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.