RLG00000027061

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0022620
Physical Location & Seq
Forward (+)
8071373 .. 8072442
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027061

Sequence Viewer

Length: 369 bp
ATGACTGCCGATGATGACGACGAGGATGATGAACTCGGTTTTCGATTCCCTGGTCAGCCGCCTCATGCAGGTACCGTTTTCTCACTCCAGCAATGGCGCCAATTGGCGGGAGCGGTCTTCCGGCGATTTCGACGACTCAGCCTACTAGATCTCCGTCTTTGGCGTATGATGGAGGTTCAAATCCAGGGATCAGTTGTTTGTAGGCCTCAGGTTAAGAAACATATTCTATCAGCAGAAACAAGGGCATTCTTCCTGAGCATGGACATATTGAAGGACTTCATCACCAAACACAATTTTCCTAATCATGTCCCTGATGAACTTGTTGAAGATTGTTCTAACTGGATTACTGTAAATCCTCTTGTAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

14.2

Weight (kDa)

5.74

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022620)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0325571
rosa_laevigata RLG00000027061
rosa_samantha Rh1AG068700 Rh1CG069000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 59
Acc65I GGTACC 1 cut(s) 71
AccB1I GGYRCC 2 cut(s) 71, 96
AccBSI CCGCTC 1 cut(s) 113
AciI CCGC 3 cut(s) 59, 107, 113
AclWI GGATC 1 cut(s) 196
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 3 cut(s) 68, 106, 259
AgsI TTSAA 3 cut(s) 179, 271, 326
AjnI CCWGG 2 cut(s) 49, 183
AlwI GGATC 1 cut(s) 196
AoxI GGCC 1 cut(s) 203
Asp700I GAANNNNTTC 1 cut(s) 275
Asp718I GGTACC 1 cut(s) 71
AspLEI GCGC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 274
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 2 cut(s) 71, 96
BbsI GAAGAC 1 cut(s) 109
BccI CCATC 1 cut(s) 163
BciT130I CCWGG 2 cut(s) 51, 185
BfaI CTAG 1 cut(s) 146
BfoI RGCGCY 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 59
BglII AGATCT 1 cut(s) 148
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 2 cut(s) 51, 185
BmiI GGNNCC 2 cut(s) 73, 98
BmrFI CCNGG 2 cut(s) 51, 185
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 1 cut(s) 71
Bpu10I CCTNAGC 1 cut(s) 254
BsaHI GRCGYC 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 49, 184
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 106, 259
Bse1I ACTGG 1 cut(s) 344
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 98
BseBI CCWGG 2 cut(s) 51, 185
BseDI CCNNGG 2 cut(s) 49, 184
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 3 cut(s) 68, 106, 259
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 3 cut(s) 151, 221, 245
BseNI ACTGG 1 cut(s) 344
BshFI GGCC 1 cut(s) 205
BshNI GGYRCC 2 cut(s) 71, 96
BsiSI CCGG 1 cut(s) 121
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 3 cut(s) 68, 106, 259
BsmFI GGGAC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 1 cut(s) 205
Bsp143I GATC 2 cut(s) 148, 188
BspACI CCGC 3 cut(s) 59, 107, 113
BspANI GGCC 1 cut(s) 205
BspCNI CTCAG 3 cut(s) 150, 220, 246
BspLI GGNNCC 2 cut(s) 73, 98
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 1 cut(s) 196
BspT107I GGYRCC 2 cut(s) 71, 96
BsrBI CCGCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 98
BsrI ACTGG 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 49, 184
BssMI GATC 2 cut(s) 148, 188
BssNI GRCGYC 1 cut(s) 97
Bst2UI CCWGG 2 cut(s) 51, 185
Bst4CI ACNGT 2 cut(s) 76, 349
BstACI GRCGYC 1 cut(s) 97
BstDEI CTNAG 3 cut(s) 137, 207, 254
BstENI CCTNNNNNAGG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 31
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 2 cut(s) 151, 191
BstMBI GATC 2 cut(s) 148, 188
BstNI CCWGG 2 cut(s) 51, 185
BstSCI CCNGG 2 cut(s) 49, 183
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 1 cut(s) 205
BtsCI GGATG 1 cut(s) 31
BveI ACCTGC 1 cut(s) 59
CfoI GCGC 1 cut(s) 99
Csp6I GTAC 1 cut(s) 72
CviAII CATG 3 cut(s) 65, 259, 305
CviJI RGCY 3 cut(s) 58, 141, 205
CviKI_1 RGCY 3 cut(s) 58, 141, 205
CviQI GTAC 1 cut(s) 72
DdeI CTNAG 3 cut(s) 137, 207, 254
DinI GGCGCC 1 cut(s) 98
DpnI GATC 2 cut(s) 150, 190
DpnII GATC 2 cut(s) 148, 188
Eco147I AGGCCT 1 cut(s) 205
Eco81I CCTNAGG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 49, 183
EgeI GGCGCC 1 cut(s) 98
EheI GGCGCC 1 cut(s) 98
FaeI CATG 3 cut(s) 68, 262, 308
FaiI YATR 7 cut(s) 66, 167, 222, 260, 266, 306, 367
FaqI GGGAC 1 cut(s) 293
FatI CATG 3 cut(s) 64, 258, 304
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 1 cut(s) 146
GlaI GCGC 1 cut(s) 98
GluI GCNGC 1 cut(s) 59
GsuI CTGGAG 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 1 cut(s) 205
HapII CCGG 1 cut(s) 121
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 97
Hin1II CATG 3 cut(s) 68, 262, 308
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 2 cut(s) 45, 135
HpaII CCGG 1 cut(s) 121
HphI GGTGA 1 cut(s) 274
Hpy188III TCNNGA 1 cut(s) 253
Hpy99I CGWCG 2 cut(s) 23, 135
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 76, 349
HpyCH4V TGCA 1 cut(s) 68
HpyF3I CTNAG 3 cut(s) 137, 207, 254
Hsp92I GRCGYC 1 cut(s) 97
Hsp92II CATG 3 cut(s) 68, 262, 308
HspAI GCGC 1 cut(s) 97
KasI GGCGCC 1 cut(s) 96
KpnI GGTACC 1 cut(s) 75
Kzo9I GATC 2 cut(s) 148, 188
LmnI GCTCC 1 cut(s) 110
MaeI CTAG 1 cut(s) 146
MalI GATC 2 cut(s) 150, 190
MbiI CCGCTC 1 cut(s) 113
MboI GATC 2 cut(s) 148, 188
MboII GAAGA 3 cut(s) 109, 241, 338
MfeI CAATTG 1 cut(s) 101
MflI RGATCY 1 cut(s) 148
MluCI AATT 2 cut(s) 101, 292
Mly113I GGCGCC 1 cut(s) 97
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 5 cut(s) 16, 72, 166, 216, 366
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 1 cut(s) 213
MspI CCGG 1 cut(s) 121
MspR9I CCNGG 2 cut(s) 51, 185
MunI CAATTG 1 cut(s) 101
Mva1269I GAATGC 1 cut(s) 245
MvaI CCWGG 2 cut(s) 51, 185
NarI GGCGCC 1 cut(s) 97
NdeII GATC 2 cut(s) 148, 188
NlaIII CATG 3 cut(s) 68, 262, 308
NlaIV GGNNCC 2 cut(s) 73, 98
PceI AGGCCT 1 cut(s) 205
PctI GAATGC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 45
PkrI GCNGC 1 cut(s) 60
PleI GAGTC 1 cut(s) 129
PluTI GGCGCC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 129
Psp6I CCWGG 2 cut(s) 49, 183
PspGI CCWGG 2 cut(s) 49, 183
PspN4I GGNNCC 2 cut(s) 73, 98
PsuI RGATCY 1 cut(s) 148
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 2 cut(s) 148, 188
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 2 cut(s) 51, 185
SetI ASST 3 cut(s) 73, 177, 213
SfoI GGCGCC 1 cut(s) 98
Sse9I AATT 2 cut(s) 101, 292
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 3 cut(s) 59, 107, 113
SspDI GGCGCC 1 cut(s) 96
SspMI CTAG 1 cut(s) 146
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 49, 183
TaaI ACNGT 2 cut(s) 76, 349
TaqI TCGA 2 cut(s) 43, 130
TasI AATT 2 cut(s) 101, 292
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TspDTI ATGAA 3 cut(s) 45, 268, 330
TspGWI ACGGA 1 cut(s) 143
XagI CCTNNNNNAGG 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.