RLG00000027114

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0020692
Physical Location & Seq
Forward (+)
8533872 .. 8534574
703 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027114

Sequence Viewer

Length: 489 bp
ATGGACCCGGAGGACTTTCTCCAGCTCAAGAACCAGTTGAAGATCACAACGGTGGACAATACGGAGTCGTTTTGCTTCAGGTCGAAGGGACTGGTACCGAACGTGCTGGGAGTGGAGGTGGACCCGATGGGCGGGCCGAGGATCCAGGAGGCGGCGATGCCGCTCTACTCGGAGAAAACCGGGTCGGAGAAGATCCATCTGCTGCAGGCGGCGGAGAAACGTTTCTTCTTTGCGTACAAGCAGCTGCTGGTGGTGTTGATGAGGAGCTTGGAGGCGAAAGGGAACCGGTTCGTGGTGGGCCCGGAATCCGGCGACGCTCTGAGCCAGATATTCCTGGAGCCGACGAAGTTTTCCGAGCTTGGAATCCGCGAAGACGTTTCTGGGGGACGGTTCGAGTCACGAGAACGTTTCGGAGAGACGGACGAGGAGGGGGTAGTTGTTGACTGGATTTTGGATGTGGAATCGGGTCTGACCCGGGGACTTGTGTAG

Protein Analysis

163

Amino Acids

18.12

Weight (kDa)

4.79

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_C PF07014 1 - 120 1.4e-39 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020692)

Species Orthologous Gene IDs
rosa_laevigata RLG00000027114
rosa_multiflora Rmu_sc0001154.1_g000011 Rmu_sc0001154.1_g000020
rosa_samantha Rh1BG323400 Rh1CG336700 Rh1DG354200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 94
AccBSI CCGCTC 1 cut(s) 163
AccII CGCG 1 cut(s) 369
AciI CCGC 6 cut(s) 132, 152, 161, 209, 212, 367
AclI AACGTT 2 cut(s) 220, 406
AclWI GGATC 3 cut(s) 136, 149, 187
AcuI CTGAAG 1 cut(s) 61
AfaI GTAC 2 cut(s) 96, 236
AfiI CCNNNNNNNGG 4 cut(s) 131, 151, 292, 308
AgeI ACCGGT 1 cut(s) 285
AgsI TTSAA 1 cut(s) 40
AjnI CCWGG 2 cut(s) 144, 333
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 4 cut(s) 25, 244, 267, 358
AluI AGCT 4 cut(s) 25, 244, 267, 358
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 3 cut(s) 136, 149, 187
AlwNI CAGNNNCTG 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 2 cut(s) 134, 298
ApaI GGGCCC 1 cut(s) 302
ApeKI GCWGC 3 cut(s) 202, 241, 244
AsiGI ACCGGT 1 cut(s) 285
Asp700I GAANNNNTTC 2 cut(s) 221, 287
Asp718I GGTACC 1 cut(s) 94
AspS9I GGNCC 5 cut(s) 4, 121, 134, 298, 299
AsuC2I CCSGG 5 cut(s) 8, 181, 302, 475, 476
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 2 cut(s) 4, 121
BaeGI GKGCMC 1 cut(s) 302
BamHI GGATCC 1 cut(s) 141
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 302
BauI CACGAG 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 189, 231, 253
BccI CCATC 2 cut(s) 121, 204
BciT130I CCWGG 2 cut(s) 146, 335
BcnI CCSGG 5 cut(s) 8, 181, 302, 475, 476
BcoDI GTCTC 1 cut(s) 410
BfmI CTRYAG 1 cut(s) 203
BisI GCNGC 6 cut(s) 153, 161, 203, 210, 242, 245
BlsI GCNGC 6 cut(s) 154, 162, 204, 211, 243, 246
Bme1390I CCNGG 7 cut(s) 8, 146, 181, 302, 335, 475, 476
Bme18I GGWCC 2 cut(s) 4, 121
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 5 cut(s) 4, 121, 134, 298, 299
BmiI GGNNCC 7 cut(s) 6, 96, 123, 143, 284, 300, 339
BmrFI CCNGG 7 cut(s) 8, 146, 181, 302, 335, 475, 476
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 378
BpmI CTGGAG 2 cut(s) 5, 356
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 5 cut(s) 8, 181, 302, 475, 476
BsaJI CCNNGG 3 cut(s) 137, 474, 475
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 151, 292, 308
Bse118I RCCGGY 1 cut(s) 285
Bse1I ACTGG 3 cut(s) 34, 96, 449
BseBI CCWGG 2 cut(s) 146, 335
BseDI CCNNGG 3 cut(s) 137, 474, 475
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 4 cut(s) 131, 151, 292, 308
BseMII CTCAG 1 cut(s) 311
BseNI ACTGG 3 cut(s) 34, 96, 449
BseRI GAGGAG 2 cut(s) 277, 440
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 3 cut(s) 189, 231, 253
BseYI CCCAGC 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 2 cut(s) 136, 300
BshNI GGYRCC 1 cut(s) 94
BshTI ACCGGT 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 6 cut(s) 8, 180, 286, 302, 309, 475
BslFI GGGAC 2 cut(s) 102, 399
BslI CCNNNNNNNGG 4 cut(s) 131, 151, 292, 308
BsmAI GTCTC 1 cut(s) 410
BsmBI CGTCTC 1 cut(s) 410
BsmFI GGGAC 2 cut(s) 102, 399
BsnI GGCC 2 cut(s) 136, 300
BsoBI CYCGRG 1 cut(s) 474
Bsp120I GGGCCC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 302
Bsp143I GATC 3 cut(s) 42, 141, 192
BspACI CCGC 6 cut(s) 132, 152, 161, 209, 212, 367
BspANI GGCC 2 cut(s) 136, 300
BspCNI CTCAG 1 cut(s) 312
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 7 cut(s) 6, 96, 123, 143, 284, 300, 339
BspMAI CTGCAG 1 cut(s) 207
BspPI GGATC 3 cut(s) 136, 149, 187
BspT107I GGYRCC 1 cut(s) 94
BsrBI CCGCTC 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 285
BsrI ACTGG 3 cut(s) 34, 96, 449
BssAI RCCGGY 1 cut(s) 285
BssECI CCNNGG 3 cut(s) 137, 474, 475
BssMI GATC 3 cut(s) 42, 141, 192
BssSI CACGAG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 2 cut(s) 146, 335
Bst4CI ACNGT 2 cut(s) 52, 390
BstC8I GCNNGC 2 cut(s) 134, 207
BstDEI CTNAG 1 cut(s) 320
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 1 cut(s) 369
BstKTI GATC 3 cut(s) 45, 144, 195
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 3 cut(s) 42, 141, 192
BstNI CCWGG 2 cut(s) 146, 335
BstSCI CCNGG 7 cut(s) 6, 144, 179, 300, 333, 473, 474
BstSFI CTRYAG 1 cut(s) 203
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 3 cut(s) 189, 231, 253
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 2 cut(s) 141, 192
BstYI RGATCY 2 cut(s) 141, 192
BsuRI GGCC 2 cut(s) 136, 300
BtgZI GCGATG 1 cut(s) 170
BtsCI GGATG 1 cut(s) 460
Cac8I GCNNGC 2 cut(s) 134, 207
CaiI CAGNNNCTG 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 285
Cfr13I GGNCC 5 cut(s) 4, 121, 134, 298, 299
Cfr9I CCCGGG 1 cut(s) 474
CseI GACGC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 95, 235
CspAI ACCGGT 1 cut(s) 285
CviJI RGCY 8 cut(s) 25, 136, 244, 267, 300, 324, 340, 358
CviKI_1 RGCY 8 cut(s) 25, 136, 244, 267, 300, 324, 340, 358
CviQI GTAC 2 cut(s) 95, 235
DdeI CTNAG 1 cut(s) 320
DpnI GATC 3 cut(s) 44, 143, 194
DpnII GATC 3 cut(s) 42, 141, 192
EciI GGCGGA 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 4, 121
Eco57I CTGAAG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 144, 333
EcoT38I GRGCYC 1 cut(s) 302
Esp3I CGTCTC 1 cut(s) 410
FaqI GGGAC 2 cut(s) 102, 399
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 6 cut(s) 153, 161, 203, 210, 242, 245
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 1 cut(s) 302
Fsp4HI GCNGC 6 cut(s) 153, 161, 203, 210, 242, 245
GluI GCNGC 6 cut(s) 153, 161, 203, 210, 242, 245
GsaI CCCAGC 1 cut(s) 110
GsuI CTGGAG 2 cut(s) 5, 356
HaeIII GGCC 2 cut(s) 136, 300
HapII CCGG 6 cut(s) 8, 180, 286, 302, 309, 475
HgaI GACGC 1 cut(s) 323
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 5 cut(s) 65, 305, 363, 395, 461
HpaII CCGG 6 cut(s) 8, 180, 286, 302, 309, 475
Hpy166II GTNNAC 3 cut(s) 55, 121, 442
Hpy188I TCNGA 6 cut(s) 172, 187, 321, 355, 413, 471
Hpy188III TCNNGA 2 cut(s) 28, 399
Hpy8I GTNNAC 3 cut(s) 55, 121, 442
Hpy99I CGWCG 2 cut(s) 317, 346
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 2 cut(s) 52, 390
HpyCH4IV ACGT 4 cut(s) 102, 220, 375, 406
HpyCH4V TGCA 1 cut(s) 205
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 4 cut(s) 102, 220, 375, 406
KpnI GGTACC 1 cut(s) 98
Kzo9I GATC 3 cut(s) 42, 141, 192
LmnI GCTCC 2 cut(s) 264, 337
Lsp1109I GCAGC 3 cut(s) 189, 231, 253
LweI GCATC 1 cut(s) 147
MaeII ACGT 4 cut(s) 102, 220, 375, 406
MaeIII GTNAC 1 cut(s) 396
MalI GATC 3 cut(s) 44, 143, 194
MbiI CCGCTC 1 cut(s) 163
MboI GATC 3 cut(s) 42, 141, 192
MboII GAAGA 4 cut(s) 52, 202, 217, 383
MflI RGATCY 2 cut(s) 141, 192
MhlI GDGCHC 1 cut(s) 302
MlyI GAGTC 2 cut(s) 74, 404
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 8 cut(s) 4, 109, 132, 142, 255, 265, 418, 421
MroXI GAANNNNTTC 2 cut(s) 221, 287
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 6 cut(s) 8, 180, 286, 302, 309, 475
MspR9I CCNGG 7 cut(s) 8, 146, 181, 302, 335, 475, 476
MvaI CCWGG 2 cut(s) 146, 335
MvnI CGCG 1 cut(s) 369
NciI CCSGG 5 cut(s) 8, 181, 302, 475, 476
NdeII GATC 3 cut(s) 42, 141, 192
NlaIV GGNNCC 7 cut(s) 6, 96, 123, 143, 284, 300, 339
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 396
OliI CACNNNNGTG 1 cut(s) 50
PdmI GAANNNNTTC 2 cut(s) 221, 287
PfeI GAWTC 3 cut(s) 305, 363, 461
PfoI TCCNGGA 2 cut(s) 144, 333
PinAI ACCGGT 1 cut(s) 285
PkrI GCNGC 6 cut(s) 154, 162, 204, 211, 243, 246
PleI GAGTC 2 cut(s) 73, 403
PpsI GAGTC 2 cut(s) 73, 403
Psp1406I AACGTT 2 cut(s) 220, 406
Psp6I CCWGG 2 cut(s) 144, 333
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 144, 333
PspN4I GGNNCC 7 cut(s) 6, 96, 123, 143, 284, 300, 339
PspOMI GGGCCC 1 cut(s) 298
PspPI GGNCC 5 cut(s) 4, 121, 134, 298, 299
PstI CTGCAG 1 cut(s) 207
PstNI CAGNNNCTG 1 cut(s) 247
PsuI RGATCY 2 cut(s) 141, 192
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 2 cut(s) 96, 236
RsaNI GTAC 2 cut(s) 95, 235
RseI CAYNNNNRTG 1 cut(s) 50
SatI GCNGC 6 cut(s) 153, 161, 203, 210, 242, 245
Sau3AI GATC 3 cut(s) 42, 141, 192
Sau96I GGNCC 5 cut(s) 4, 121, 134, 298, 299
SchI GAGTC 2 cut(s) 74, 404
ScrFI CCNGG 7 cut(s) 8, 146, 181, 302, 335, 475, 476
SduI GDGCHC 1 cut(s) 302
SfaNI GCATC 1 cut(s) 147
SfcI CTRYAG 1 cut(s) 203
SinI GGWCC 2 cut(s) 4, 121
SmaI CCCGGG 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SsiI CCGC 6 cut(s) 132, 152, 161, 209, 212, 367
StyD4I CCNGG 7 cut(s) 6, 144, 179, 300, 333, 473, 474
TaaI ACNGT 2 cut(s) 52, 390
TaiI ACGT 4 cut(s) 105, 223, 378, 409
TaqI TCGA 2 cut(s) 83, 393
TauI GCSGC 3 cut(s) 155, 163, 212
TfiI GAWTC 3 cut(s) 305, 363, 461
TseFI GTSAC 1 cut(s) 396
TseI GCWGC 3 cut(s) 202, 241, 244
Tsp45I GTSAC 1 cut(s) 396
TspGWI ACGGA 2 cut(s) 77, 434
TspMI CCCGGG 1 cut(s) 474
VpaK11BI GGWCC 2 cut(s) 4, 121
XmaI CCCGGG 1 cut(s) 474
XmnI GAANNNNTTC 2 cut(s) 221, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.