Rh1CG336700

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0020692
Physical Location & Seq
Reverse (-)
61113212 .. 61115251
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG336700.1

Sequence Viewer

Length: 450 bp
ATGGACCCGGAGGACTTTCTCCGGCTCAAGAACCAGTTGAAGATCAAAACGGGACTGGTGGAGATCACAAGGCTCTGTAAGGACTTGAAGCACAAGGTACCGAACGTGCTGGGAGTGGAGGTGGACCCGATGGGGGGGCCGAGGATCCAGGAGGCGGCGATGAGTCTCTACTCGGCGGAAACCGGGTCGGAGAAGATCCATCTGATGCAGGCTATCGAGTCGGCGGCGAAACGTTTCTTCTTCGAGTACAAGCAGGTGCTGGTGGTGTTGATGGGGAGCTTGGAGGCGGAAGGGAACCGGGTCGTGGTGGGCCCGGAGTCCGGCGACGCGCTGAGCCAGATATTGACTGGATTTTGGACATGGACCCGGGTTGTGGAATCGGGTTTGACCCGGGGACTTCCGTCATTAAAGGAAAACTTAATTCTTTTTGCTCCTTTTTTGGGTTTTTAG

Protein Analysis

149

Amino Acids

16.55

Weight (kDa)

6.14

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_C PF07014 1 - 115 1.2e-41 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020692)

Species Orthologous Gene IDs
rosa_laevigata RLG00000027114
rosa_multiflora Rmu_sc0001154.1_g000011 Rmu_sc0001154.1_g000020
rosa_samantha Rh1BG323400 Rh1CG336700 Rh1DG354200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 1 cut(s) 329
AciI CCGC 4 cut(s) 155, 176, 224, 287
AclI AACGTT 1 cut(s) 232
AclWI GGATC 3 cut(s) 139, 152, 190
AfaI GTAC 2 cut(s) 99, 248
AfiI CCNNNNNNNGG 7 cut(s) 133, 134, 154, 304, 320, 373, 440
AgsI TTSAA 2 cut(s) 40, 88
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 1 cut(s) 279
AluI AGCT 1 cut(s) 279
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 3 cut(s) 139, 152, 190
AlwNI CAGNNNCTG 1 cut(s) 259
Ama87I CYCGRG 2 cut(s) 366, 390
AoxI GGCC 2 cut(s) 137, 310
ApaI GGGCCC 1 cut(s) 314
Asp700I GAANNNNTTC 1 cut(s) 233
Asp718I GGTACC 1 cut(s) 97
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 6 cut(s) 4, 124, 137, 310, 311, 363
AsuC2I CCSGG 8 cut(s) 8, 184, 299, 314, 367, 368, 391, 392
AvaI CYCGRG 2 cut(s) 366, 390
AvaII GGWCC 3 cut(s) 4, 124, 363
BaeGI GKGCMC 1 cut(s) 314
BamHI GGATCC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 314
BccI CCATC 3 cut(s) 124, 207, 265
BciT130I CCWGG 1 cut(s) 149
BcnI CCSGG 8 cut(s) 8, 184, 299, 314, 367, 368, 391, 392
BcoDI GTCTC 1 cut(s) 170
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 2 cut(s) 156, 225
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 2 cut(s) 157, 226
Bme1390I CCNGG 9 cut(s) 8, 149, 184, 299, 314, 367, 368, 391, 392
Bme18I GGWCC 3 cut(s) 4, 124, 363
BmeT110I CYCGRG 2 cut(s) 366, 390
BmgT120I GGNCC 6 cut(s) 4, 124, 137, 310, 311, 363
BmiI GGNNCC 8 cut(s) 6, 99, 126, 138, 146, 296, 312, 365
BmrFI CCNGG 9 cut(s) 8, 149, 184, 299, 314, 367, 368, 391, 392
BmsI GCATC 1 cut(s) 195
BoxI GACNNNNGTC 1 cut(s) 400
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 8 cut(s) 8, 184, 299, 314, 367, 368, 391, 392
BsaJI CCNNGG 4 cut(s) 140, 366, 390, 391
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 7 cut(s) 133, 134, 154, 304, 320, 373, 440
Bse1I ACTGG 3 cut(s) 34, 60, 352
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 4 cut(s) 140, 366, 390, 391
BseLI CCNNNNNNNGG 7 cut(s) 133, 134, 154, 304, 320, 373, 440
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 3 cut(s) 34, 60, 352
BseSI GKGCMC 1 cut(s) 314
BseYI CCCAGC 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 2 cut(s) 139, 312
BshNI GGYRCC 1 cut(s) 97
BsiHKCI CYCGRG 2 cut(s) 366, 390
BsiSI CCGG 8 cut(s) 8, 22, 183, 298, 314, 321, 367, 391
BslFI GGGAC 2 cut(s) 66, 408
BslI CCNNNNNNNGG 7 cut(s) 133, 134, 154, 304, 320, 373, 440
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 66, 408
BsnI GGCC 2 cut(s) 139, 312
BsoBI CYCGRG 2 cut(s) 366, 390
Bsp120I GGGCCC 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 4 cut(s) 42, 63, 144, 195
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 4 cut(s) 155, 176, 224, 287
BspANI GGCC 2 cut(s) 139, 312
BspCNI CTCAG 1 cut(s) 324
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 8 cut(s) 6, 99, 126, 138, 146, 296, 312, 365
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 3 cut(s) 139, 152, 190
BspT107I GGYRCC 1 cut(s) 97
BsrI ACTGG 3 cut(s) 34, 60, 352
BssECI CCNNGG 4 cut(s) 140, 366, 390, 391
BssMI GATC 4 cut(s) 42, 63, 144, 195
Bst2UI CCWGG 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 332
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 4 cut(s) 45, 66, 147, 198
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 4 cut(s) 42, 63, 144, 195
BstNI CCWGG 1 cut(s) 149
BstPAI GACNNNNGTC 1 cut(s) 400
BstSCI CCNGG 9 cut(s) 6, 147, 182, 297, 312, 365, 366, 389, 390
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 1 cut(s) 329
BstX2I RGATCY 2 cut(s) 144, 195
BstYI RGATCY 2 cut(s) 144, 195
BsuRI GGCC 2 cut(s) 139, 312
BtgZI GCGATG 1 cut(s) 173
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 6 cut(s) 4, 124, 137, 310, 311, 363
Cfr9I CCCGGG 2 cut(s) 366, 390
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 98, 247
CviAII CATG 1 cut(s) 360
CviJI RGCY 7 cut(s) 25, 73, 139, 212, 279, 312, 336
CviKI_1 RGCY 7 cut(s) 25, 73, 139, 212, 279, 312, 336
CviQI GTAC 2 cut(s) 98, 247
DdeI CTNAG 1 cut(s) 332
DpnI GATC 4 cut(s) 44, 65, 146, 197
DpnII GATC 4 cut(s) 42, 63, 144, 195
EciI GGCGGA 2 cut(s) 191, 302
Eco24I GRGCYC 1 cut(s) 314
Eco47I GGWCC 3 cut(s) 4, 124, 363
Eco88I CYCGRG 2 cut(s) 366, 390
EcoRII CCWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 314
FaeI CATG 1 cut(s) 363
FaiI YATR 1 cut(s) 361
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FaqI GGGAC 2 cut(s) 66, 408
FatI CATG 1 cut(s) 359
Fnu4HI GCNGC 2 cut(s) 156, 225
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 2 cut(s) 156, 225
GlaI GCGC 1 cut(s) 330
GluI GCNGC 2 cut(s) 156, 225
GsaI CCCAGC 1 cut(s) 113
HaeIII GGCC 2 cut(s) 139, 312
HapII CCGG 8 cut(s) 8, 22, 183, 298, 314, 321, 367, 391
HgaI GACGC 1 cut(s) 335
HhaI GCGC 1 cut(s) 331
Hin1II CATG 1 cut(s) 363
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HinfI GANTC 4 cut(s) 163, 218, 317, 377
HpaII CCGG 8 cut(s) 8, 22, 183, 298, 314, 321, 367, 391
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 190, 204
Hpy188III TCNNGA 1 cut(s) 28
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 1 cut(s) 284
HpyCH4IV ACGT 2 cut(s) 105, 232
HpyCH4V TGCA 1 cut(s) 208
HpyF3I CTNAG 1 cut(s) 332
HpySE526I ACGT 2 cut(s) 105, 232
Hsp92II CATG 1 cut(s) 363
HspAI GCGC 1 cut(s) 329
KpnI GGTACC 1 cut(s) 101
Kzo9I GATC 4 cut(s) 42, 63, 144, 195
LmnI GCTCC 2 cut(s) 276, 436
LweI GCATC 1 cut(s) 195
MaeII ACGT 2 cut(s) 105, 232
MalI GATC 4 cut(s) 44, 65, 146, 197
MboI GATC 4 cut(s) 42, 63, 144, 195
MboII GAAGA 4 cut(s) 52, 205, 229, 232
MflI RGATCY 2 cut(s) 144, 195
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 1 cut(s) 420
MlyI GAGTC 3 cut(s) 172, 227, 326
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 5 cut(s) 4, 112, 135, 145, 277
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 2 cut(s) 407, 419
MspI CCGG 8 cut(s) 8, 22, 183, 298, 314, 321, 367, 391
MspR9I CCNGG 9 cut(s) 8, 149, 184, 299, 314, 367, 368, 391, 392
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 329
NciI CCSGG 8 cut(s) 8, 184, 299, 314, 367, 368, 391, 392
NdeII GATC 4 cut(s) 42, 63, 144, 195
NlaIII CATG 1 cut(s) 363
NlaIV GGNNCC 8 cut(s) 6, 99, 126, 138, 146, 296, 312, 365
NmeAIII GCCGAG 2 cut(s) 152, 165
PaqCI CACCTGC 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 1 cut(s) 377
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 2 cut(s) 157, 226
PleI GAGTC 3 cut(s) 171, 226, 325
PpsI GAGTC 3 cut(s) 171, 226, 325
PshAI GACNNNNGTC 1 cut(s) 400
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 8 cut(s) 6, 99, 126, 138, 146, 296, 312, 365
PspOMI GGGCCC 1 cut(s) 310
PspPI GGNCC 6 cut(s) 4, 124, 137, 310, 311, 363
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 2 cut(s) 144, 195
RsaI GTAC 2 cut(s) 99, 248
RsaNI GTAC 2 cut(s) 98, 247
SaqAI TTAA 2 cut(s) 407, 419
SatI GCNGC 2 cut(s) 156, 225
Sau3AI GATC 4 cut(s) 42, 63, 144, 195
Sau96I GGNCC 6 cut(s) 4, 124, 137, 310, 311, 363
SchI GAGTC 3 cut(s) 172, 227, 326
ScrFI CCNGG 9 cut(s) 8, 149, 184, 299, 314, 367, 368, 391, 392
SduI GDGCHC 1 cut(s) 314
SetI ASST 6 cut(s) 99, 108, 123, 235, 258, 281
SfaNI GCATC 1 cut(s) 195
SinI GGWCC 3 cut(s) 4, 124, 363
SmaI CCCGGG 2 cut(s) 368, 392
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 1 cut(s) 420
SsiI CCGC 4 cut(s) 155, 176, 224, 287
StyD4I CCNGG 9 cut(s) 6, 147, 182, 297, 312, 365, 366, 389, 390
TaiI ACGT 2 cut(s) 108, 235
TaqI TCGA 2 cut(s) 216, 243
TasI AATT 1 cut(s) 420
TatI WGTACW 1 cut(s) 246
TauI GCSGC 2 cut(s) 158, 227
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 2 cut(s) 407, 419
Tru9I TTAA 2 cut(s) 407, 419
TspGWI ACGGA 1 cut(s) 390
TspMI CCCGGG 2 cut(s) 366, 390
VpaK11BI GGWCC 3 cut(s) 4, 124, 363
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmaI CCCGGG 2 cut(s) 366, 390
XmnI GAANNNNTTC 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.