RLG00000029515

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0019850
Physical Location & Seq
Forward (+)
39726599 .. 39736618
10020 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029515

Sequence Viewer

Length: 795 bp
ATGACCTGCATTCTTAGCCTAAGTCCTCATGTCCGGGCGCTTAGGCTTTTGTCCGGTATTGTTAACTCGCTCTATTCATTCTATTGGGACTTGAGACGCCTGTACCGATTAATCAAGCTCGAGAATGGCGTCATTGCATTACTAGTTCACGATCCTGAGATTGGAGAATCCCAACTCTCCGAGGGCTCTGAGGAAGACGACAATGGTGAAGGAGAAGATGAAGATGAACTGAATAAGAAAAAGGGAGGGGATTCTCGGACTAACAAGGCAGCAGCAGCATTGTGGGTTGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACAGGGGCTTGCACACTTTCTAGAACACATGCTTTTCATGGGGAGTACAGAATTTCCAGATGAAAATGAGAGTTTAACCAGGTTCTGCAGAACGGTGCTTGCCACCTTGAACAACTTCAATGCCATACAGCCTCACCTGGTCACCCTTTTAATAGATTTGCAAGAGGATACTGGGAACATGGAATTTAGATTTGCAGAGGAGCAGCCTCAAGCTGATTATATGCCTCAGAACTTGCAGTGCGAACCTTGGTTTAGGTCGCATTATACTGAGGAAGATGTACCTCCATCTTTGATAGATTTGTGGAAGGACCCTCCAGAAGTTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTACTACTGATTTTTCCATTTGTTCTGATGGTCTTGATACTACAGTTACAGCTTCTCCGAGATGTATATTGGATGAGCCATTGGCAAAGTTCTGGTGCAAACTTGATAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

265

Amino Acids

29.85

Weight (kDa)

4.41

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 86 - 130 1e-12 Insulinase (Peptidase family M16)
Peptidase_M16_M PF16187 183 - 263 2.7e-17 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019850)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0333071 RchiOBHm_Chr1g0334031
rosa_laevigata RLG00000029515
rosa_multiflora Rmu_sc0000026.1_g000011 Rmu_sc0002722.1_g000012
rosa_samantha Rh1AG130100 Rh1CG123900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 14
AclWI GGATC 1 cut(s) 146
AcsI RAATTY 2 cut(s) 371, 503
AcyI GRCGYC 2 cut(s) 97, 129
AfaI GTAC 4 cut(s) 104, 367, 600, 790
AfiI CCNNNNNNNGG 2 cut(s) 161, 316
AgsI TTSAA 2 cut(s) 430, 439
AhlI ACTAGT 1 cut(s) 142
AjnI CCWGG 2 cut(s) 398, 456
AluBI AGCT 4 cut(s) 118, 300, 533, 731
AluI AGCT 4 cut(s) 118, 300, 533, 731
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 405
Ama87I CYCGRG 1 cut(s) 119
ApeKI GCWGC 5 cut(s) 269, 272, 275, 297, 523
ApoI RAATTY 2 cut(s) 371, 503
AseI ATTAAT 1 cut(s) 110
Asp700I GAANNNNTTC 2 cut(s) 434, 674
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 628
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 218, 446, 454
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 628
AxyI CCTNAGG 1 cut(s) 315
BaeI ACNNNNGTAYC 4 cut(s) 86, 119, 708, 741
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 201
BbvI GCAGC 5 cut(s) 281, 284, 287, 309, 535
BccI CCATC 2 cut(s) 613, 701
BciT130I CCWGG 2 cut(s) 400, 458
BciVI GTATCC 1 cut(s) 481
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 88
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 2 cut(s) 143, 341
BfmI CTRYAG 2 cut(s) 406, 720
BfoI RGCGCY 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 5 cut(s) 270, 273, 276, 298, 524
BlsI GCNGC 5 cut(s) 271, 274, 277, 299, 525
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 3 cut(s) 35, 400, 458
Bme18I GGWCC 1 cut(s) 628
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 1 cut(s) 630
BmrFI CCNGG 3 cut(s) 35, 400, 458
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 664
BmuI ACTGGG 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 112, 513
BpuMI CCSGG 1 cut(s) 35
BsaHI GRCGYC 2 cut(s) 97, 129
BsaJI CCNNGG 2 cut(s) 180, 566
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 316
Bse1I ACTGG 1 cut(s) 496
Bse21I CCTNAGG 1 cut(s) 315
Bse3DI GCAATG 2 cut(s) 132, 650
BseBI CCWGG 2 cut(s) 400, 458
BseDI CCNNGG 2 cut(s) 180, 566
BseGI GGATG 1 cut(s) 757
BseLI CCNNNNNNNGG 2 cut(s) 161, 316
BseMI GCAATG 2 cut(s) 132, 650
BseMII CTCAG 5 cut(s) 147, 180, 306, 560, 579
BseNI ACTGG 1 cut(s) 496
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 5 cut(s) 281, 284, 287, 309, 535
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 2 cut(s) 34, 54
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 161, 316
BsmAI GTCTC 1 cut(s) 88
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 1 cut(s) 151
BspCNI CTCAG 5 cut(s) 148, 181, 307, 559, 580
BspLI GGNNCC 1 cut(s) 630
BspMAI CTGCAG 1 cut(s) 410
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 146
BsrDI GCAATG 2 cut(s) 132, 650
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 180, 566
BssMI GATC 1 cut(s) 151
BssNI GRCGYC 2 cut(s) 97, 129
BssT1I CCWWGG 1 cut(s) 566
Bst2UI CCWGG 2 cut(s) 400, 458
Bst4CI ACNGT 2 cut(s) 415, 724
BstACI GRCGYC 2 cut(s) 97, 129
BstC8I GCNNGC 2 cut(s) 330, 420
BstDEI CTNAG 8 cut(s) 14, 20, 41, 156, 189, 315, 546, 588
BstEII GGTNACC 1 cut(s) 460
BstF5I GGATG 1 cut(s) 757
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 2 cut(s) 15, 275
BstNI CCWGG 2 cut(s) 400, 458
BstNSI RCATGY 1 cut(s) 352
BstPI GGTNACC 1 cut(s) 460
BstSCI CCNGG 3 cut(s) 33, 398, 456
BstSFI CTRYAG 2 cut(s) 406, 720
BstV1I GCAGC 5 cut(s) 281, 284, 287, 309, 535
BstV2I GAAGAC 1 cut(s) 201
Bsu36I CCTNAGG 1 cut(s) 315
BsuI GTATCC 1 cut(s) 481
BtsCI GGATG 1 cut(s) 757
BtsI GCAGTG 1 cut(s) 563
BtsIMutI CAGTG 1 cut(s) 563
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 2 cut(s) 330, 420
CaiI CAGNNNCTG 1 cut(s) 405
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 628
CseI GACGC 2 cut(s) 105, 118
CsiI ACCWGGT 2 cut(s) 398, 456
Csp6I GTAC 4 cut(s) 103, 366, 599, 789
CviAII CATG 4 cut(s) 29, 349, 358, 499
CviQI GTAC 4 cut(s) 103, 366, 599, 789
DdeI CTNAG 8 cut(s) 14, 20, 41, 156, 189, 315, 546, 588
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 566
Eco24I GRGCYC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 628
Eco81I CCTNAGG 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 119
Eco91I GGTNACC 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 628
EcoO65I GGTNACC 1 cut(s) 460
EcoRII CCWGG 2 cut(s) 398, 456
EcoT14I CCWWGG 1 cut(s) 566
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 566
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 4 cut(s) 32, 352, 361, 502
FaiI YATR 9 cut(s) 30, 350, 359, 446, 500, 540, 542, 585, 746
FaqI GGGAC 1 cut(s) 101
FatI CATG 4 cut(s) 28, 348, 357, 498
Fnu4HI GCNGC 5 cut(s) 270, 273, 276, 298, 524
FokI GGATG 1 cut(s) 764
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 270, 273, 276, 298, 524
FspBI CTAG 2 cut(s) 143, 341
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 270, 273, 276, 298, 524
GsuI CTGGAG 1 cut(s) 618
HaeII RGCGCY 1 cut(s) 41
HapII CCGG 2 cut(s) 34, 54
HgaI GACGC 2 cut(s) 105, 118
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 2 cut(s) 97, 129
Hin1II CATG 4 cut(s) 32, 352, 361, 502
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 2 cut(s) 167, 251
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 2 cut(s) 34, 54
HphI GGTGA 3 cut(s) 218, 446, 454
Hpy166II GTNNAC 2 cut(s) 64, 148
Hpy188I TCNGA 7 cut(s) 181, 190, 258, 307, 549, 706, 738
Hpy188III TCNNGA 8 cut(s) 121, 149, 155, 314, 341, 377, 635, 713
Hpy8I GTNNAC 2 cut(s) 64, 148
HpyAV CCTTC 2 cut(s) 203, 619
HpyCH4III ACNGT 2 cut(s) 415, 724
HpyCH4V TGCA 9 cut(s) 9, 137, 332, 408, 481, 515, 556, 655, 777
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 275
HpyF3I CTNAG 8 cut(s) 14, 20, 41, 156, 189, 315, 546, 588
Hsp92I GRCGYC 2 cut(s) 97, 129
Hsp92II CATG 4 cut(s) 32, 352, 361, 502
HspAI GCGC 1 cut(s) 38
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 1 cut(s) 520
Lsp1109I GCAGC 5 cut(s) 281, 284, 287, 309, 535
LweI GCATC 1 cut(s) 664
MabI ACCWGGT 2 cut(s) 398, 456
MaeI CTAG 2 cut(s) 143, 341
MaeIII GTNAC 2 cut(s) 460, 724
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 4 cut(s) 206, 227, 233, 605
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 2 cut(s) 371, 503
MmeI TCCRAC 1 cut(s) 268
MroXI GAANNNNTTC 2 cut(s) 434, 674
MseI TTAA 4 cut(s) 63, 110, 395, 470
MspI CCGG 2 cut(s) 34, 54
MspR9I CCNGG 3 cut(s) 35, 400, 458
Mva1269I GAATGC 1 cut(s) 9
MvaI CCWGG 2 cut(s) 400, 458
MwoI GCNNNNNNNGC 2 cut(s) 15, 275
NciI CCSGG 1 cut(s) 35
NdeII GATC 1 cut(s) 151
NlaIII CATG 4 cut(s) 32, 352, 361, 502
NlaIV GGNNCC 1 cut(s) 630
NmuCI GTSAC 1 cut(s) 460
NspI RCATGY 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 119
PcsI WCGNNNNNNNCGW 2 cut(s) 103, 126
PctI GAATGC 1 cut(s) 9
PdmI GAANNNNTTC 2 cut(s) 434, 674
PfeI GAWTC 2 cut(s) 167, 251
PkrI GCNGC 5 cut(s) 271, 274, 277, 299, 525
PpuMI RGGWCCY 1 cut(s) 628
PshBI ATTAAT 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 628
Psp6I CCWGG 2 cut(s) 398, 456
PspEI GGTNACC 1 cut(s) 460
PspGI CCWGG 2 cut(s) 398, 456
PspN4I GGNNCC 1 cut(s) 630
PspPI GGNCC 1 cut(s) 628
PspPPI RGGWCCY 1 cut(s) 628
PstI CTGCAG 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 405
RsaI GTAC 4 cut(s) 104, 367, 600, 790
RsaNI GTAC 4 cut(s) 103, 366, 599, 789
SaqAI TTAA 4 cut(s) 63, 110, 395, 470
SatI GCNGC 5 cut(s) 270, 273, 276, 298, 524
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 628
ScaI AGTACT 1 cut(s) 790
ScrFI CCNGG 3 cut(s) 35, 400, 458
SduI GDGCHC 1 cut(s) 188
SexAI ACCWGGT 2 cut(s) 398, 456
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 2 cut(s) 406, 720
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 1 cut(s) 628
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 3 cut(s) 91, 119, 528
SmoI CTYRAG 3 cut(s) 91, 119, 528
SpeI ACTAGT 1 cut(s) 142
Sse9I AATT 2 cut(s) 371, 503
SspMI CTAG 2 cut(s) 143, 341
StyD4I CCNGG 3 cut(s) 33, 398, 456
StyI CCWWGG 1 cut(s) 566
TaaI ACNGT 2 cut(s) 415, 724
TaqI TCGA 1 cut(s) 120
TasI AATT 2 cut(s) 371, 503
TatI WGTACW 2 cut(s) 365, 788
TfiI GAWTC 2 cut(s) 167, 251
Tru1I TTAA 4 cut(s) 63, 110, 395, 470
Tru9I TTAA 4 cut(s) 63, 110, 395, 470
TscAI CASTG 1 cut(s) 563
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 5 cut(s) 269, 272, 275, 297, 523
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 7 cut(s) 66, 234, 240, 346, 396, 639, 667
TspRI CASTG 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 628
VspI ATTAAT 1 cut(s) 110
XapI RAATTY 2 cut(s) 371, 503
XbaI TCTAGA 1 cut(s) 340
XceI RCATGY 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 119
XmnI GAANNNNTTC 2 cut(s) 434, 674
XspI CTAG 2 cut(s) 143, 341
ZrmI AGTACT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.