RLG00000029908

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0020481
Physical Location & Seq
Forward (+)
51125607 .. 51126377
771 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029908

Sequence Viewer

Length: 627 bp
ATGGTATGGAGATGGAAAGGAAGGAATTGGAACAAAGTCAAGCTTTTACTATGGCGGGCTCGCCACGGATTCCTACCCTGTGGTGAGCATCTGTTGAGGTGCAAAATTGGTCCTAATAGGGGGTGTGAACGGTGTGGAGGAGACTGTGTTACATTGTTTTTGGGACTGTCTGCAGGTGAAGAAAACCTGGAAGTATACTTGGCATGGGGTGGTGGGGCGTGTGTAAGTTGTGGAATGAACGGGGGTTTCAAGATTTGCTTGCTCATGTTCTTAAGTGCTCATGAGCAGAAACAGTTGCAGGTCACTGAGAGGTTGGATGCTATCCAAGTGGAACAGCAGGTCCAGCGCTGGATGCGTTTGGACCCTCAGTTTTTCAGACCGAATTTTGATGGGGCAAGTGACTTGAAGAAGGGAAGATCTTGGCTGGGTGCGGTGGTTAGAGAGGAAGCTGGGAAGTTAACTGGTGCGTTAGCTGTCCCAGTTCAGGCACAATTATCTCCGGCAGCAGCAGTGGCGCTGGCACTCTGGAAAGGGATGCGTCTGTATAAGCAGCTGGGAGTGCGTAAGATGTTGGAGGGTGATGCCTTGAATGTAAGCAATGCCTTGCAGTCATCTCAGGGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

209

Amino Acids

23.04

Weight (kDa)

9.45

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 128 - 205 2.1e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020481)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0329231
rosa_laevigata RLG00000029908
rosa_multiflora Rmu_sc0000156.1_g000010 Rmu_sc0014673.1_g000002
rosa_roxburghii Rroxscaffold_2G00140680 Rroxscaffold_4G00278730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 164
Acc36I ACCTGC 3 cut(s) 164, 289, 328
AccI GTMKAC 1 cut(s) 195
AciI CCGC 2 cut(s) 55, 431
AcsI RAATTY 1 cut(s) 382
AfeI AGCGCT 1 cut(s) 347
AfiI CCNNNNNNNGG 2 cut(s) 119, 484
AflII CTTAAG 1 cut(s) 271
AgsI TTSAA 3 cut(s) 250, 406, 589
AjnI CCWGG 1 cut(s) 186
AloI GAACNNNNNNTCC 2 cut(s) 324, 356
AluBI AGCT 4 cut(s) 43, 449, 473, 553
AluI AGCT 4 cut(s) 43, 449, 473, 553
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 1 cut(s) 135
Aor51HI AGCGCT 1 cut(s) 347
ApeKI GCWGC 3 cut(s) 503, 506, 550
ApoI RAATTY 1 cut(s) 382
AspLEI GCGC 2 cut(s) 348, 517
AspS9I GGNCC 3 cut(s) 110, 340, 361
AsuHPI GGTGA 3 cut(s) 95, 188, 590
AvaII GGWCC 3 cut(s) 110, 340, 361
BanII GRGCYC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 3 cut(s) 515, 518, 562
BccI CCATC 2 cut(s) 6, 383
BciT130I CCWGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 2 cut(s) 349, 518
BfrI CTTAAG 1 cut(s) 271
BfuAI ACCTGC 3 cut(s) 164, 289, 328
BglII AGATCT 1 cut(s) 416
BisI GCNGC 3 cut(s) 504, 507, 551
BlsI GCNGC 3 cut(s) 505, 508, 552
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 3 cut(s) 110, 340, 361
BmgT120I GGNCC 3 cut(s) 110, 340, 361
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 1 cut(s) 188
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 5 cut(s) 97, 307, 342, 525, 571
BmuI ACTGGG 1 cut(s) 473
BsaJI CCNNGG 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 484
Bse1I ACTGG 2 cut(s) 466, 479
Bse3DI GCAATG 1 cut(s) 604
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 3 cut(s) 322, 357, 540
BseLI CCNNNNNNNGG 2 cut(s) 119, 484
BseMI GCAATG 1 cut(s) 604
BseMII CTCAG 2 cut(s) 297, 380
BseNI ACTGG 2 cut(s) 466, 479
BseRI GAGGAG 1 cut(s) 153
BseXI GCAGC 3 cut(s) 515, 518, 562
BseYI CCCAGC 3 cut(s) 424, 449, 553
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 500
BslFI GGGAC 2 cut(s) 177, 461
BslI CCNNNNNNNGG 2 cut(s) 119, 484
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 177, 461
Bsp1286I GDGCHC 2 cut(s) 61, 280
Bsp143I GATC 2 cut(s) 416, 620
BspACI CCGC 2 cut(s) 55, 431
BspCNI CTCAG 2 cut(s) 298, 379
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 363
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 3 cut(s) 164, 289, 328
BspTI CTTAAG 1 cut(s) 271
BsrDI GCAATG 1 cut(s) 604
BsrI ACTGG 2 cut(s) 466, 479
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 2 cut(s) 416, 620
BssNAI GTATAC 1 cut(s) 196
Bst1107I GTATAC 1 cut(s) 196
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 4 cut(s) 132, 146, 168, 294
BstAFI CTTAAG 1 cut(s) 271
BstC8I GCNNGC 4 cut(s) 57, 61, 260, 519
BstDEI CTNAG 4 cut(s) 306, 366, 615, 624
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 3 cut(s) 322, 357, 540
BstH2I RGCGCY 2 cut(s) 349, 518
BstHHI GCGC 2 cut(s) 348, 517
BstKTI GATC 2 cut(s) 419, 623
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 416, 620
BstMWI GCNNNNNNNGC 4 cut(s) 343, 352, 512, 559
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 171
BstV1I GCAGC 3 cut(s) 515, 518, 562
BstX2I RGATCY 2 cut(s) 416, 620
BstYI RGATCY 2 cut(s) 416, 620
BstZ17I GTATAC 1 cut(s) 196
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 322, 357, 540
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 2 cut(s) 303, 516
BveI ACCTGC 3 cut(s) 164, 289, 328
Cac8I GCNNGC 4 cut(s) 57, 61, 260, 519
CciI TCATGA 1 cut(s) 280
CfoI GCGC 2 cut(s) 348, 517
Cfr13I GGNCC 3 cut(s) 110, 340, 361
CseI GACGC 1 cut(s) 527
CviAII CATG 3 cut(s) 204, 265, 281
CviJI RGCY 6 cut(s) 43, 59, 424, 449, 473, 553
CviKI_1 RGCY 6 cut(s) 43, 59, 424, 449, 473, 553
DdeI CTNAG 4 cut(s) 306, 366, 615, 624
DpnI GATC 2 cut(s) 418, 622
DpnII GATC 2 cut(s) 416, 620
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 3 cut(s) 110, 340, 361
Eco47III AGCGCT 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 61
FaeI CATG 3 cut(s) 207, 268, 284
FaiI YATR 7 cut(s) 7, 52, 196, 205, 266, 282, 546
FalI AAGNNNNNCTT 4 cut(s) 27, 59, 242, 274
FaqI GGGAC 2 cut(s) 177, 461
FatI CATG 3 cut(s) 203, 264, 280
FauI CCCGC 1 cut(s) 48
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 3 cut(s) 504, 507, 551
FokI GGATG 3 cut(s) 329, 364, 547
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 3 cut(s) 504, 507, 551
GlaI GCGC 2 cut(s) 347, 516
GluI GCNGC 3 cut(s) 504, 507, 551
GsaI CCCAGC 3 cut(s) 428, 453, 557
HaeII RGCGCY 2 cut(s) 349, 518
HapII CCGG 1 cut(s) 500
HgaI GACGC 1 cut(s) 527
HhaI GCGC 2 cut(s) 348, 517
Hin1II CATG 3 cut(s) 207, 268, 284
Hin6I GCGC 2 cut(s) 346, 515
HinP1I GCGC 2 cut(s) 346, 515
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 1 cut(s) 69
HpaI GTTAAC 1 cut(s) 459
HpaII CCGG 1 cut(s) 500
HphI GGTGA 3 cut(s) 95, 188, 590
Hpy166II GTNNAC 3 cut(s) 128, 196, 459
Hpy188I TCNGA 1 cut(s) 377
Hpy188III TCNNGA 3 cut(s) 250, 281, 526
Hpy8I GTNNAC 3 cut(s) 128, 196, 459
HpyAV CCTTC 2 cut(s) 15, 403
HpyCH4III ACNGT 4 cut(s) 132, 146, 168, 294
HpyCH4V TGCA 4 cut(s) 102, 173, 298, 607
HpyF10VI GCNNNNNNNGC 4 cut(s) 343, 352, 512, 559
HpyF3I CTNAG 4 cut(s) 306, 366, 615, 624
Hsp92II CATG 3 cut(s) 207, 268, 284
HspAI GCGC 2 cut(s) 346, 515
KspAI GTTAAC 1 cut(s) 459
Kzo9I GATC 2 cut(s) 416, 620
Lsp1109I GCAGC 3 cut(s) 515, 518, 562
LweI GCATC 5 cut(s) 97, 307, 342, 525, 571
MaeIII GTNAC 3 cut(s) 148, 301, 398
MalI GATC 2 cut(s) 418, 622
MboI GATC 2 cut(s) 416, 620
MboII GAAGA 3 cut(s) 191, 418, 426
MflI RGATCY 2 cut(s) 416, 620
MhlI GDGCHC 2 cut(s) 61, 280
MluCI AATT 4 cut(s) 25, 105, 382, 491
MmeI TCCRAC 2 cut(s) 294, 552
MnlI CCTC 6 cut(s) 90, 131, 303, 375, 436, 568
MseI TTAA 2 cut(s) 272, 458
MspA1I CMGCKG 1 cut(s) 553
MspCI CTTAAG 1 cut(s) 271
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 188
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 4 cut(s) 343, 352, 512, 559
NdeII GATC 2 cut(s) 416, 620
NlaIII CATG 3 cut(s) 207, 268, 284
NlaIV GGNNCC 1 cut(s) 363
NmuCI GTSAC 2 cut(s) 301, 398
PagI TCATGA 1 cut(s) 280
PaqCI CACCTGC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 69
PkrI GCNGC 3 cut(s) 505, 508, 552
Psp6I CCWGG 1 cut(s) 186
PspFI CCCAGC 3 cut(s) 424, 449, 553
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 363
PspPI GGNCC 3 cut(s) 110, 340, 361
PstI CTGCAG 1 cut(s) 175
PsuI RGATCY 2 cut(s) 416, 620
PvuII CAGCTG 1 cut(s) 553
SaqAI TTAA 2 cut(s) 272, 458
SatI GCNGC 3 cut(s) 504, 507, 551
Sau3AI GATC 2 cut(s) 416, 620
Sau96I GGNCC 3 cut(s) 110, 340, 361
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 2 cut(s) 61, 280
SfaNI GCATC 5 cut(s) 97, 307, 342, 525, 571
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 3 cut(s) 110, 340, 361
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 4 cut(s) 25, 105, 382, 491
SsiI CCGC 2 cut(s) 55, 431
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 132, 146, 168, 294
TaqII GACCGA 1 cut(s) 394
TasI AATT 4 cut(s) 25, 105, 382, 491
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 2 cut(s) 272, 458
Tru9I TTAA 2 cut(s) 272, 458
TscAI CASTG 2 cut(s) 310, 516
TseFI GTSAC 2 cut(s) 301, 398
TseI GCWGC 3 cut(s) 503, 506, 550
Tsp45I GTSAC 2 cut(s) 301, 398
TspDTI ATGAA 1 cut(s) 251
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 2 cut(s) 310, 516
Vha464I CTTAAG 1 cut(s) 271
VpaK11BI GGWCC 3 cut(s) 110, 340, 361
XapI RAATTY 1 cut(s) 382
XmiI GTMKAC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.