Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0009908

Physical Location & Seq

Genomic Coordinates Forward (+)

62461244 .. 62463480

Gene Structure 2237 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030494

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGAGGCATCCACCAGTTCACCTTCTGAAGCAAATGAACATTTTTATCATAAAAAGCTTCATGATCTTGTTTCTGAGCTCTGTAACTGTTAGAGTTAACCTTTGTTCTCCAAATACGCCTTCCTTAAACACACTTCCACTTGAAGGTCATTTCAACTTTCACGAAGTTCAGCATGCAGCCCGAGATTTTGGCAACAGGTACCAGTTCCTCCCTATGGCAGTACTACATCCAAAAACGGTTTCGGACATAGCCACTTTAATCAAGCATATTTGGGATATTGGTCCTCGTTCAGAGCTCACAGCTGCTGCTAGAGGCCATGGCCACTCACTCCACGGTCAGTCACAAGCCCACAGAGGAGTTGTCATCAATATGGAATCACTTAAGGGGCCTGAAATCCAGGTTTACACTGGCAACTTTCCATATGCAGATGTCTCTGGCGGTGAGTTGTGGATAAATATCCTGCATGAAACCTTGAAATATGGGTTAGCACCAAAATCATGGACAGACTACCTACATCTAACTGTTGGTGGTACTCTGTCTAATGCTGGGATCAGTGGGCAGGCATTCCGACATGGCCCCCAGATCAGCAATGTCCGCCAGCTGGAAGTTGTTACAGGAAAAGGGGAGATTGTAACTTGTTCAGAGAAGCAGAACGACGACCTATTTCATGGTGTTCTTGGAGGACTTGGTCAGTTTGGCATCATAACCCGGGCAAGAATATTGTTGGAAGCAGCACCTGCAAGGGTAAAATGGATTAGAGTGCTGTATTCAGATTTCGCAGCATTTACCCGAGATCAGGAGCATCTAATATCTGGTGAAAATACATTTAATTACGTCGAAGGATTTGTCATAATAAACAGGAGTGGTCTGCTGAACAGTTGGAGATCATCTTTCAATCCAAAAGACCCAGTTCAGGCCAGCCAGTTCAAGTCAGATGGAAGAACTCTATTCTGCCTAGAAATGGCCAAATACTTCAACCTGGACAAGATCGATATAATAAATCAGGAAGTTGACAAGTCATTGTCTGAATTGAGCTACATCCCTTCCACGCTATTTGAGTCAGACGTTACATACACAGATTTCTTGGACAGGGTACATATATCTGAGATGAAACTACGCTCAAAAGATCTGTGGGACGTTCCACACCCATGGCTCAATCTTCTTATACCAAAAAGCAAAATTCATACTTTTGCTCGAGGAGTGTTTGGCAACATCCATACAGAAACAAGCAATGGCCCCATCCTCATCTATCCAGTTAACAGATCAAAGTGGGACAACAGAACTTCCGTAGTTATACCGGAGGAACATATTTTTTACTTAGTGGCATTCCTTAACTCTGCAGTTCCCTCTTCAACTGGAACTGATGGTTTAGAACACATCTTAATTCAGAACAAAAGAATTCTAGAATTCTGTGAAACAGCCAATCTTGGGGTCAAGCAATATCTACCGCATTACACCAAACAAGAAGAATGGCAGGCCCACTTTGGCCCCCACTGGGAAAAATTTGTCCAAAGAAAATCTACTTATGACCCTTTGGCAATACTTGCTCCAGGACAAAGAATATTTCAAAAGGCAATACCCTTCTCATCACAGTCGCCATCTAGTATAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009308 GO:0009653 GO:0009690 GO:0009791 GO:0009823 GO:0009888 GO:0009987 GO:0010016 GO:0010103 GO:0010374 GO:0010817 GO:0016491 GO:0016645 GO:0019139 GO:0032501 GO:0032502 GO:0034754 GO:0042445 GO:0042447 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048367 GO:0048507 GO:0048731 GO:0048856 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0090558 GO:0090626 GO:0090698 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 map00908

Pfam Domains

Protein Families

Cytokin-bind FAD_binding_4

Protein Analysis

538

Amino Acids

60.67

Weight (kDa)

8.2

Isoelectric Point (pI)

38.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	71 - 214	3.5e-17	FAD binding domain
Cytokin-bind	PF09265	246 - 522	1.3e-120	Cytokinin dehydrogenase 1, FAD and cytokinin binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63440
fragaria_vesca	FvH4_3g03260 FvH4_7g02150 FvH4_7g02150
malus_domestica	MD07G1026600.v1.1
prunus_persica	Prupe.2G026700_v2.0.a1 Prupe.2G026700_v2.0.a1
pyrus_communis	pycom02g25060 pycom07g02010
rosa_chinensis	RchiOBHm_Chr1g0319331
rosa_laevigata	RLG00000030494
rosa_multiflora	Rmu_sc0003338.1_g000006 Rmu_sc0014506.1_g000010
rosa_roxburghii	Rroxscaffold_4G00328570
rosa_rugosa	Rorug01G0026800 Rorug01G0026900
rosa_samantha	Rh1AG039000 Rh1BG035800 Rh1CG038000 Rh1DG031900
rosa_wichuraiana	Rw1G003120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	745
Acc36I	ACCTGC	1 cut(s)	745
Acc65I	GGTACC	1 cut(s)	198
AccB1I	GGYRCC	1 cut(s)	198
AciI	CCGC	3 cut(s)	438, 595, 1448
AclWI	GGATC	1 cut(s)	557
AcoI	YGGCCR	2 cut(s)	319, 963
AcsI	RAATTY	4 cut(s)	1179, 1398, 1406, 1502
AcuI	CTGAAG	1 cut(s)	47
AfaI	GTAC	4 cut(s)	200, 222, 532, 1095
AfiI	CCNNNNNNNGG	8 cut(s)	143, 214, 601, 796, 913, 961, 1428, 1495
AflII	CTTAAG	1 cut(s)	380
AgsI	TTSAA	8 cut(s)	143, 154, 475, 895, 928, 976, 1353, 1568
AjnI	CCWGG	3 cut(s)	396, 978, 1549
AjuI	GAANNNNNNNTTGG	4 cut(s)	103, 135, 223, 255
AluBI	AGCT	6 cut(s)	57, 78, 295, 302, 601, 1035
AluI	AGCT	6 cut(s)	57, 78, 295, 302, 601, 1035
Alw21I	GWGCWC	2 cut(s)	80, 297
Alw26I	GTCTC	1 cut(s)	436
AlwI	GGATC	1 cut(s)	557
AlwNI	CAGNNNCTG	2 cut(s)	305, 737
Ama87I	CYCGRG	4 cut(s)	180, 708, 789, 1194
AoxI	GGCC	9 cut(s)	313, 319, 386, 574, 915, 963, 1234, 1476, 1486
ApeKI	GCWGC	5 cut(s)	176, 302, 305, 731, 779
ApoI	RAATTY	4 cut(s)	1179, 1398, 1406, 1502
ArsI	GACNNNNNNTTYG	2 cut(s)	831, 863
Asp718I	GGTACC	1 cut(s)	198
AspS9I	GGNCC	6 cut(s)	281, 386, 575, 1235, 1477, 1487
AsuC2I	CCSGG	2 cut(s)	709, 710
AsuHPI	GGTGA	3 cut(s)	11, 452, 827
AvaI	CYCGRG	4 cut(s)	180, 708, 789, 1194
AvaII	GGWCC	1 cut(s)	281
BalI	TGGCCA	2 cut(s)	321, 965
BanI	GGYRCC	1 cut(s)	198
BanII	GRGCYC	2 cut(s)	80, 297
Bbv12I	GWGCWC	2 cut(s)	80, 297
BbvI	GCAGC	5 cut(s)	188, 289, 292, 743, 791
BccI	CCATC	4 cut(s)	929, 1247, 1358, 1606
BciT130I	CCWGG	3 cut(s)	398, 980, 1551
BcnI	CCSGG	2 cut(s)	709, 710
BcoDI	GTCTC	1 cut(s)	436
BfaI	CTAG	4 cut(s)	309, 956, 1403, 1602
BfmI	CTRYAG	1 cut(s)	1338
BfrI	CTTAAG	1 cut(s)	380
BfuAI	ACCTGC	1 cut(s)	745
BglII	AGATCT	1 cut(s)	1126
BisI	GCNGC	5 cut(s)	177, 303, 306, 732, 780
BlsI	GCNGC	5 cut(s)	178, 304, 307, 733, 781
BmcAI	AGTACT	1 cut(s)	222
Bme1390I	CCNGG	5 cut(s)	398, 709, 710, 980, 1551
Bme18I	GGWCC	1 cut(s)	281
BmeT110I	CYCGRG	4 cut(s)	180, 708, 789, 1194
BmgT120I	GGNCC	6 cut(s)	281, 386, 575, 1235, 1477, 1487
BmiI	GGNNCC	5 cut(s)	200, 387, 577, 1237, 1489
BmrFI	CCNGG	5 cut(s)	398, 709, 710, 980, 1551
BmrI	ACTGGG	2 cut(s)	902, 1504
BmsI	GCATC	3 cut(s)	16, 708, 811
BmuI	ACTGGG	2 cut(s)	902, 1504
BpmI	CTGGAG	1 cut(s)	1533
BpuMI	CCSGG	2 cut(s)	709, 710
Bsa29I	ATCGAT	1 cut(s)	990
BsaBI	GATNNNNATC	1 cut(s)	455
BsaJI	CCNNGG	4 cut(s)	316, 331, 708, 1148
BsaWI	WCCGGW	1 cut(s)	1297
Bsc4I	CCNNNNNNNGG	8 cut(s)	143, 214, 601, 796, 913, 961, 1428, 1495
Bse1I	ACTGG	8 cut(s)	14, 202, 412, 908, 922, 1253, 1360, 1499
Bse3DI	GCAATG	2 cut(s)	595, 1237
Bse8I	GATNNNNATC	1 cut(s)	455
BseBI	CCWGG	3 cut(s)	398, 980, 1551
BseCI	ATCGAT	1 cut(s)	990
BseDI	CCNNGG	4 cut(s)	316, 331, 708, 1148
BseGI	GGATG	5 cut(s)	7, 226, 1038, 1212, 1239
BseJI	GATNNNNATC	1 cut(s)	455
BseLI	CCNNNNNNNGG	8 cut(s)	143, 214, 601, 796, 913, 961, 1428, 1495
BseMI	GCAATG	2 cut(s)	595, 1237
BseMII	CTCAG	2 cut(s)	65, 1095
BseNI	ACTGG	8 cut(s)	14, 202, 412, 908, 922, 1253, 1360, 1499
BseRI	GAGGAG	2 cut(s)	369, 1212
BseXI	GCAGC	5 cut(s)	188, 289, 292, 743, 791
BseYI	CCCAGC	1 cut(s)	545
BshFI	GGCC	9 cut(s)	315, 321, 388, 576, 917, 965, 1236, 1478, 1488
BshNI	GGYRCC	1 cut(s)	198
BshVI	ATCGAT	1 cut(s)	990
BsiHKAI	GWGCWC	2 cut(s)	80, 297
BsiHKCI	CYCGRG	4 cut(s)	180, 708, 789, 1194
BsiSI	CCGG	2 cut(s)	709, 1298
BslFI	GGGAC	2 cut(s)	1148, 1286
BslI	CCNNNNNNNGG	8 cut(s)	143, 214, 601, 796, 913, 961, 1428, 1495
BsmAI	GTCTC	1 cut(s)	436
BsmFI	GGGAC	2 cut(s)	1148, 1286
BsmI	GAATGC	2 cut(s)	563, 1325
BsnI	GGCC	9 cut(s)	315, 321, 388, 576, 917, 965, 1236, 1478, 1488
BsoBI	CYCGRG	4 cut(s)	180, 708, 789, 1194
Bsp1286I	GDGCHC	2 cut(s)	80, 297
Bsp143I	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
Bsp19I	CCATGG	2 cut(s)	316, 1148
BspACI	CCGC	3 cut(s)	438, 595, 1448
BspANI	GGCC	9 cut(s)	315, 321, 388, 576, 917, 965, 1236, 1478, 1488
BspCNI	CTCAG	2 cut(s)	66, 1096
BspDI	ATCGAT	1 cut(s)	990
BspHI	TCATGA	1 cut(s)	60
BspLI	GGNNCC	5 cut(s)	200, 387, 577, 1237, 1489
BspMAI	CTGCAG	1 cut(s)	1342
BspMI	ACCTGC	1 cut(s)	745
BspPI	GGATC	1 cut(s)	557
BspT107I	GGYRCC	1 cut(s)	198
BspTI	CTTAAG	1 cut(s)	380
BsrDI	GCAATG	2 cut(s)	595, 1237
BsrI	ACTGG	8 cut(s)	14, 202, 412, 908, 922, 1253, 1360, 1499
BssECI	CCNNGG	4 cut(s)	316, 331, 708, 1148
BssMI	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
BssT1I	CCWWGG	2 cut(s)	316, 1148
Bst2UI	CCWGG	3 cut(s)	398, 980, 1551
Bst4CI	ACNGT	6 cut(s)	88, 238, 335, 523, 878, 1593
Bst6I	CTCTTC	1 cut(s)	1354
BstAFI	CTTAAG	1 cut(s)	380
BstAPI	GCANNNNNTGC	2 cut(s)	737, 1544
BstC8I	GCNNGC	5 cut(s)	174, 561, 599, 919, 1476
BstDEI	CTNAG	3 cut(s)	74, 1104, 1318
BstDSI	CCRYGG	3 cut(s)	316, 331, 1148
BstF5I	GGATG	5 cut(s)	7, 226, 1038, 1212, 1239
BstKTI	GATC	8 cut(s)	66, 552, 585, 796, 887, 990, 1129, 1265
BstMAI	GTCTC	1 cut(s)	436
BstMBI	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
BstMWI	GCNNNNNNNGC	3 cut(s)	594, 737, 1544
BstNI	CCWGG	3 cut(s)	398, 980, 1551
BstNSI	RCATGY	1 cut(s)	176
BstSCI	CCNGG	5 cut(s)	396, 707, 708, 978, 1549
BstSFI	CTRYAG	1 cut(s)	1338
BstV1I	GCAGC	5 cut(s)	188, 289, 292, 743, 791
BstX2I	RGATCY	1 cut(s)	1126
BstXI	CCANNNNNNTGG	2 cut(s)	498, 1149
BstYI	RGATCY	1 cut(s)	1126
Bsu15I	ATCGAT	1 cut(s)	990
BsuRI	GGCC	9 cut(s)	315, 321, 388, 576, 917, 965, 1236, 1478, 1488
BsuTUI	ATCGAT	1 cut(s)	990
BtgI	CCRYGG	3 cut(s)	316, 331, 1148
BtsCI	GGATG	5 cut(s)	7, 226, 1038, 1212, 1239
BtsIMutI	CAGTG	3 cut(s)	405, 559, 1492
BveI	ACCTGC	1 cut(s)	745
Cac8I	GCNNGC	5 cut(s)	174, 561, 599, 919, 1476
CaiI	CAGNNNCTG	2 cut(s)	305, 737
CciI	TCATGA	1 cut(s)	60
Cfr13I	GGNCC	6 cut(s)	281, 386, 575, 1235, 1477, 1487
Cfr9I	CCCGGG	1 cut(s)	708
ClaI	ATCGAT	1 cut(s)	990
Csp6I	GTAC	4 cut(s)	199, 221, 531, 1094
CspCI	CAANNNNNGTGG	2 cut(s)	1036, 1071
CviAII	CATG	8 cut(s)	61, 173, 317, 464, 498, 572, 668, 1149
CviQI	GTAC	4 cut(s)	199, 221, 531, 1094
DdeI	CTNAG	3 cut(s)	74, 1104, 1318
DpnI	GATC	8 cut(s)	65, 551, 584, 795, 886, 989, 1128, 1264
DpnII	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
EaeI	YGGCCR	2 cut(s)	319, 963
Eam1104I	CTCTTC	1 cut(s)	1354
EarI	CTCTTC	1 cut(s)	1354
EciI	GGCGGA	1 cut(s)	584
Ecl136II	GAGCTC	2 cut(s)	78, 295
Eco130I	CCWWGG	2 cut(s)	316, 1148
Eco24I	GRGCYC	2 cut(s)	80, 297
Eco47I	GGWCC	1 cut(s)	281
Eco53kI	GAGCTC	2 cut(s)	78, 295
Eco57I	CTGAAG	1 cut(s)	47
Eco88I	CYCGRG	4 cut(s)	180, 708, 789, 1194
EcoICRI	GAGCTC	2 cut(s)	78, 295
EcoO109I	RGGNCCY	1 cut(s)	386
EcoRI	GAATTC	2 cut(s)	1398, 1406
EcoRII	CCWGG	3 cut(s)	396, 978, 1549
EcoT14I	CCWWGG	2 cut(s)	316, 1148
EcoT38I	GRGCYC	2 cut(s)	80, 297
ErhI	CCWWGG	2 cut(s)	316, 1148
FaeI	CATG	8 cut(s)	64, 176, 320, 467, 501, 575, 671, 1152
FaqI	GGGAC	2 cut(s)	1148, 1286
FatI	CATG	8 cut(s)	60, 172, 316, 463, 497, 571, 667, 1148
FauNDI	CATATG	1 cut(s)	421
Fnu4HI	GCNGC	5 cut(s)	177, 303, 306, 732, 780
FokI	GGATG	4 cut(s)	213, 1025, 1199, 1226
FriOI	GRGCYC	2 cut(s)	80, 297
Fsp4HI	GCNGC	5 cut(s)	177, 303, 306, 732, 780
FspBI	CTAG	4 cut(s)	309, 956, 1403, 1602
GluI	GCNGC	5 cut(s)	177, 303, 306, 732, 780
GsaI	CCCAGC	1 cut(s)	549
GsuI	CTGGAG	1 cut(s)	1533
HaeIII	GGCC	9 cut(s)	315, 321, 388, 576, 917, 965, 1236, 1478, 1488
HapII	CCGG	2 cut(s)	709, 1298
Hin1II	CATG	8 cut(s)	64, 176, 320, 467, 501, 575, 671, 1152
HincII	GTYRAC	3 cut(s)	97, 1012, 1258
HindII	GTYRAC	3 cut(s)	97, 1012, 1258
HindIII	AAGCTT	1 cut(s)	55
HinfI	GANTC	2 cut(s)	374, 1058
HpaI	GTTAAC	2 cut(s)	97, 1258
HpaII	CCGG	2 cut(s)	709, 1298
HphI	GGTGA	3 cut(s)	11, 452, 827
Hpy166II	GTNNAC	5 cut(s)	19, 97, 403, 1012, 1258
Hpy188III	TCNNGA	5 cut(s)	61, 161, 797, 1004, 1403
Hpy8I	GTNNAC	5 cut(s)	19, 97, 403, 1012, 1258
Hpy99I	CGWCG	2 cut(s)	659, 839
HpyAV	CCTTC	6 cut(s)	32, 129, 137, 833, 1053, 1591
HpyCH4III	ACNGT	6 cut(s)	88, 238, 335, 523, 878, 1593
HpyCH4IV	ACGT	3 cut(s)	834, 1065, 1137
HpyCH4V	TGCA	5 cut(s)	176, 425, 463, 740, 1340
HpyF10VI	GCNNNNNNNGC	3 cut(s)	594, 737, 1544
HpyF3I	CTNAG	3 cut(s)	74, 1104, 1318
HpySE526I	ACGT	3 cut(s)	834, 1065, 1137
Hsp92II	CATG	8 cut(s)	64, 176, 320, 467, 501, 575, 671, 1152
KpnI	GGTACC	1 cut(s)	202
KspAI	GTTAAC	2 cut(s)	97, 1258
Kzo9I	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
LmnI	GCTCC	2 cut(s)	799, 1552
Lsp1109I	GCAGC	5 cut(s)	188, 289, 292, 743, 791
LweI	GCATC	3 cut(s)	16, 708, 811
MaeI	CTAG	4 cut(s)	309, 956, 1403, 1602
MaeII	ACGT	3 cut(s)	834, 1065, 1137
MaeIII	GTNAC	5 cut(s)	82, 339, 610, 631, 1066
MalI	GATC	8 cut(s)	65, 551, 584, 795, 886, 989, 1128, 1264
MboI	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
MboII	GAAGA	4 cut(s)	951, 1151, 1341, 1478
MflI	RGATCY	1 cut(s)	1126
MhlI	GDGCHC	2 cut(s)	80, 297
MlsI	TGGCCA	2 cut(s)	321, 965
MluCI	AATT	7 cut(s)	829, 1028, 1179, 1383, 1398, 1406, 1502
MluNI	TGGCCA	2 cut(s)	321, 965
MlyI	GAGTC	1 cut(s)	1067
MmeI	TCCRAC	3 cut(s)	592, 705, 860
MnlI	CCTC	9 cut(s)	218, 294, 305, 347, 674, 1190, 1253, 1294, 1357
Mox20I	TGGCCA	2 cut(s)	321, 965
MscI	TGGCCA	2 cut(s)	321, 965
MseI	TTAA	8 cut(s)	96, 125, 257, 381, 828, 1257, 1332, 1382
MslI	CAYNNNNRTG	2 cut(s)	368, 1147
Msp20I	TGGCCA	2 cut(s)	321, 965
MspA1I	CMGCKG	2 cut(s)	302, 601
MspCI	CTTAAG	1 cut(s)	380
MspI	CCGG	2 cut(s)	709, 1298
MspR9I	CCNGG	5 cut(s)	398, 709, 710, 980, 1551
Mva1269I	GAATGC	2 cut(s)	563, 1325
MvaI	CCWGG	3 cut(s)	398, 980, 1551
MwoI	GCNNNNNNNGC	3 cut(s)	594, 737, 1544
NciI	CCSGG	2 cut(s)	709, 710
NcoI	CCATGG	2 cut(s)	316, 1148
NdeI	CATATG	1 cut(s)	421
NdeII	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
NlaIII	CATG	8 cut(s)	64, 176, 320, 467, 501, 575, 671, 1152
NlaIV	GGNNCC	5 cut(s)	200, 387, 577, 1237, 1489
NmuCI	GTSAC	1 cut(s)	339
NspI	RCATGY	1 cut(s)	176
PaeI	GCATGC	1 cut(s)	176
PaeR7I	CTCGAG	1 cut(s)	1194
PagI	TCATGA	1 cut(s)	60
PaqCI	CACCTGC	1 cut(s)	745
PctI	GAATGC	2 cut(s)	563, 1325
PfeI	GAWTC	1 cut(s)	374
PflFI	GACNNNGTC	1 cut(s)	687
PfoI	TCCNGGA	1 cut(s)	1549
PkrI	GCNGC	5 cut(s)	178, 304, 307, 733, 781
PleI	GAGTC	1 cut(s)	1066
PpsI	GAGTC	1 cut(s)	1066
Psp124BI	GAGCTC	2 cut(s)	80, 297
Psp6I	CCWGG	3 cut(s)	396, 978, 1549
PspFI	CCCAGC	1 cut(s)	545
PspGI	CCWGG	3 cut(s)	396, 978, 1549
PspN4I	GGNNCC	5 cut(s)	200, 387, 577, 1237, 1489
PspPI	GGNCC	6 cut(s)	281, 386, 575, 1235, 1477, 1487
PspXI	VCTCGAGB	1 cut(s)	1194
PstI	CTGCAG	1 cut(s)	1342
PstNI	CAGNNNCTG	2 cut(s)	305, 737
PsuI	RGATCY	1 cut(s)	1126
PsyI	GACNNNGTC	1 cut(s)	687
PvuII	CAGCTG	2 cut(s)	302, 601
RsaI	GTAC	4 cut(s)	200, 222, 532, 1095
RsaNI	GTAC	4 cut(s)	199, 221, 531, 1094
RseI	CAYNNNNRTG	2 cut(s)	368, 1147
SacI	GAGCTC	2 cut(s)	80, 297
SaqAI	TTAA	8 cut(s)	96, 125, 257, 381, 828, 1257, 1332, 1382
SatI	GCNGC	5 cut(s)	177, 303, 306, 732, 780
Sau3AI	GATC	8 cut(s)	63, 549, 582, 793, 884, 987, 1126, 1262
Sau96I	GGNCC	6 cut(s)	281, 386, 575, 1235, 1477, 1487
ScaI	AGTACT	1 cut(s)	222
SchI	GAGTC	1 cut(s)	1067
ScrFI	CCNGG	5 cut(s)	398, 709, 710, 980, 1551
SduI	GDGCHC	2 cut(s)	80, 297
SfaNI	GCATC	3 cut(s)	16, 708, 811
SfcI	CTRYAG	1 cut(s)	1338
Sfr274I	CTCGAG	1 cut(s)	1194
SinI	GGWCC	1 cut(s)	281
SlaI	CTCGAG	1 cut(s)	1194
SmaI	CCCGGG	1 cut(s)	710
SmiMI	CAYNNNNRTG	2 cut(s)	368, 1147
SmlI	CTYRAG	2 cut(s)	380, 1194
SmoI	CTYRAG	2 cut(s)	380, 1194
SphI	GCATGC	1 cut(s)	176
Sse9I	AATT	7 cut(s)	829, 1028, 1179, 1383, 1398, 1406, 1502
SsiI	CCGC	3 cut(s)	438, 595, 1448
SspI	AATATT	2 cut(s)	720, 1563
SspMI	CTAG	4 cut(s)	309, 956, 1403, 1602
SstI	GAGCTC	2 cut(s)	80, 297
StyD4I	CCNGG	5 cut(s)	396, 707, 708, 978, 1549
StyI	CCWWGG	2 cut(s)	316, 1148
TaaI	ACNGT	6 cut(s)	88, 238, 335, 523, 878, 1593
TaiI	ACGT	3 cut(s)	837, 1068, 1140
TaqI	TCGA	3 cut(s)	837, 990, 1195
TasI	AATT	7 cut(s)	829, 1028, 1179, 1383, 1398, 1406, 1502
TatI	WGTACW	1 cut(s)	220
TfiI	GAWTC	1 cut(s)	374
Tru1I	TTAA	8 cut(s)	96, 125, 257, 381, 828, 1257, 1332, 1382
Tru9I	TTAA	8 cut(s)	96, 125, 257, 381, 828, 1257, 1332, 1382
TscAI	CASTG	3 cut(s)	412, 559, 1499
TseFI	GTSAC	1 cut(s)	339
TseI	GCWGC	5 cut(s)	176, 302, 305, 731, 779
Tsp45I	GTSAC	1 cut(s)	339
TspDTI	ATGAA	6 cut(s)	49, 50, 480, 656, 1124, 1172
TspGWI	ACGGA	1 cut(s)	1276
TspMI	CCCGGG	1 cut(s)	708
TspRI	CASTG	3 cut(s)	412, 559, 1499
Tth111I	GACNNNGTC	1 cut(s)	687
Vha464I	CTTAAG	1 cut(s)	380
VpaK11BI	GGWCC	1 cut(s)	281
XapI	RAATTY	4 cut(s)	1179, 1398, 1406, 1502
XbaI	TCTAGA	1 cut(s)	1402
XceI	RCATGY	1 cut(s)	176
XcmI	CCANNNNNNNNNTGG	1 cut(s)	404
XhoI	CTCGAG	1 cut(s)	1194
XmaI	CCCGGG	1 cut(s)	708
XspI	CTAG	4 cut(s)	309, 956, 1403, 1602
ZrmI	AGTACT	1 cut(s)	222

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.