RLG00000033128

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019813
Physical Location & Seq
Forward (+)
23614307 .. 23617794
3488 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033128

Sequence Viewer

Length: 1374 bp
ATGAGTAGCAAGTCTTACGCTCTGCTCTGGGATGACCCTGTTTTTGGTGAGTGCAAGCTTATAAACCCTATTACTTACAACAATGGAACTATTGTTTATAAAGGAAGAAATGTTTCGGCTCATAGTCTCTTTGTCATTGGGACTTGTAACGGTCTCGTATGTCTTGCTCCTGACAGTTATGTTGATTCTCCCGTTTTAATTTGGAACCCATCTATTAGAAAGATTGTGATTTTGCCTAGCCCACCATCTAGTAGGAGTAGTGGTAAAAGATACGCTTTTGGTTACGATTCGCGTACCAATGACTATAAGGTGTTGATAATTGTGAGGGTTTGCGATGATTTCACAAAGACGATGGAAACTCGAGGTGAGATTTACTCGCTAGCTAGGGGCACCTGGAAGAGTCTCAGTGCAGCTGCTACTCCTGTAGATTTTGTGCCTCCTGGTTTGCATAATATTGCACGGGCATTGGTCAATGTCAATGGCTCTCCACATTGGTCTGGTGGAATGTATCGCCCCTGGGGAAACAGGGGCAACTGCATTATGTCGTTTGATATGGTCAGTGAAGTGATTTGCAAGATAGAGGTGCCTGAAGCTTTGAGGGAACAGACATGCTGGGTTTCAAAACATGGGGACTGCCTCGCCTTGTATATGCTCGACAGGCTTTCCCCAAGGCCATCGTTTGACTTCAACATTTGGGTGATGAAAGAGTATGGTGTGGCGGAATCATGGACTAAGTTAACCCTACGGCAGGAGGGATATCTTTATATGCATGGATTGTTGTATTGTAGGAGTGAAGATCTCGTGTTCGAAAGATATGATGGAAAGTTGCATACTGTGGATTGTAGGACTGGTCGAGTAAAAGATTATGGAATTCGTGCAGAGTACTACTTCATGGATTATTTTGTAGAGAGCATTCTCTTACTTGGCCAAGCCAATGCTATATCACTCACCAAAATCCCAAAACCCACTGACCCCAATCACCACCACAACGGCACAATTCCACCACTTGTGGTGGTGGGCTCAGCCAATGCAGACATCCATGTTGAGATTGAGAGACTCCCCAAGGAGGGTGAGACCATCTCAACCAAAACCGGTCAGACCCTGGCTGGGGGAAAAGGAGCCAACCAGGCTGCCTGTGTTGGCAAGCCGGCTTACCCAACCTACTTTGTGGGACAGGTGGGTGAGGATGCCCATGGCAAACGAGTCATTGAGGCCCTCAGGGGTGGTGGGGTCCACCTGGATCACCTGACTAGTAGTGATGAGCAAAACTCCATTATCGTTGTGGGTGGTGCCAACATGAAGTACTGTTGGCCTGAGAGCCTGAGTGATGAGGATTTGAGGGTTGTGAGGAGTGCTGGGATTGTTTTGCTTTAA

Protein Analysis

458

Amino Acids

50.67

Weight (kDa)

6.89

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 42 - 248 4e-19 F-box associated beta propeller domain
FBA_3 PF08268 42 - 246 2.3e-15 F-box associated beta propeller domain
PfkB PF00294 336 - 422 1.1e-09 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019813)

Species Orthologous Gene IDs
pyrus_communis pycom03g19930
rosa_chinensis RchiOBHm_Chr5g0028931
rosa_laevigata RLG00000033128
rosa_multiflora Rmu_sc0040883.1_g000001 Rmu_sc0040884.1_g000001
rosa_samantha Rh5CG222900 Rh5DG205900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 62, 99
AccB1I GGYRCC 3 cut(s) 389, 583, 1289
AccII CGCG 1 cut(s) 292
AciI CCGC 1 cut(s) 719
AclWI GGATC 1 cut(s) 1248
AcoI YGGCCR 1 cut(s) 925
AcsI RAATTY 1 cut(s) 870
AcuI CTGAAG 1 cut(s) 609
AfaI GTAC 3 cut(s) 295, 884, 1304
AfiI CCNNNNNNNGG 6 cut(s) 44, 748, 1066, 1067, 1107, 1108
AgeI ACCGGT 1 cut(s) 1091
AgsI TTSAA 2 cut(s) 621, 688
AhlI ACTAGT 1 cut(s) 1250
AjnI CCWGG 6 cut(s) 392, 439, 515, 1101, 1125, 1236
AluBI AGCT 4 cut(s) 58, 383, 413, 593
AluI AGCT 4 cut(s) 58, 383, 413, 593
Alw26I GTCTC 5 cut(s) 131, 158, 407, 1048, 1067
AlwI GGATC 1 cut(s) 1248
AlwNI CAGNNNCTG 1 cut(s) 1102
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 4 cut(s) 671, 925, 1212, 1310
ApeKI GCWGC 3 cut(s) 410, 413, 1130
ApoI RAATTY 1 cut(s) 870
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AsiGI ACCGGT 1 cut(s) 1091
Asp700I GAANNNNTTC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 1213, 1231
AsuHPI GGTGA 8 cut(s) 59, 377, 709, 940, 971, 1082, 1193, 1235
AsuII TTCGAA 1 cut(s) 807
AsuNHI GCTAGC 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 360
AvaII GGWCC 1 cut(s) 1231
AxyI CCTNAGG 1 cut(s) 1217
BaeGI GKGCMC 1 cut(s) 392
BalI TGGCCA 1 cut(s) 927
BanI GGYRCC 3 cut(s) 389, 583, 1289
BanII GRGCYC 1 cut(s) 1022
BauI CACGAG 1 cut(s) 800
BbvI GCAGC 3 cut(s) 400, 422, 1117
BccI CCATC 6 cut(s) 217, 253, 346, 682, 812, 1085
BceAI ACGGC 2 cut(s) 761, 1006
BciT130I CCWGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
BcoDI GTCTC 5 cut(s) 131, 158, 407, 1048, 1067
BcuI ACTAGT 1 cut(s) 1250
BfaI CTAG 5 cut(s) 237, 249, 380, 384, 1251
BfmI CTRYAG 1 cut(s) 423
BglI GCCNNNNNGGC 1 cut(s) 1127
BglII AGATCT 1 cut(s) 796
BisI GCNGC 3 cut(s) 411, 414, 1131
BlpI GCTNAGC 1 cut(s) 1021
BlsI GCNGC 3 cut(s) 412, 415, 1132
BmcAI AGTACT 2 cut(s) 884, 1304
Bme1390I CCNGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
Bme18I GGWCC 1 cut(s) 1231
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 2 cut(s) 1213, 1231
BmiI GGNNCC 6 cut(s) 206, 391, 585, 1120, 1232, 1291
BmrFI CCNGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
BmsI GCATC 1 cut(s) 1177
BmtI GCTAGC 1 cut(s) 383
BplI GAGNNNNNCTC 6 cut(s) 359, 391, 1064, 1096, 1253, 1285
Bpu1102I GCTNAGC 1 cut(s) 1021
Bpu14I TTCGAA 1 cut(s) 807
BsaI GGTCTC 2 cut(s) 158, 1067
BsaJI CCNNGG 6 cut(s) 515, 516, 668, 1062, 1101, 1192
BsaWI WCCGGW 1 cut(s) 1091
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 6 cut(s) 44, 748, 1066, 1067, 1107, 1108
Bse118I RCCGGY 2 cut(s) 1091, 1147
Bse1I ACTGG 1 cut(s) 853
Bse21I CCTNAGG 1 cut(s) 1217
BseBI CCWGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
BseDI CCNNGG 6 cut(s) 515, 516, 668, 1062, 1101, 1192
BseGI GGATG 3 cut(s) 37, 1035, 1192
BseLI CCNNNNNNNGG 6 cut(s) 44, 748, 1066, 1067, 1107, 1108
BseMII CTCAG 5 cut(s) 418, 1035, 1231, 1305, 1313
BseNI ACTGG 1 cut(s) 853
BseRI GAGGAG 1 cut(s) 1363
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 3 cut(s) 400, 422, 1117
BseYI CCCAGC 3 cut(s) 612, 1106, 1355
BsgI GTGCAG 2 cut(s) 429, 897
Bsh1236I CGCG 1 cut(s) 292
BshFI GGCC 4 cut(s) 673, 927, 1214, 1312
BshNI GGYRCC 3 cut(s) 389, 583, 1289
BshTI ACCGGT 1 cut(s) 1091
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 2 cut(s) 1092, 1148
BslFI GGGAC 3 cut(s) 154, 644, 1185
BslI CCNNNNNNNGG 6 cut(s) 44, 748, 1066, 1067, 1107, 1108
BsmAI GTCTC 5 cut(s) 131, 158, 407, 1048, 1067
BsmFI GGGAC 3 cut(s) 154, 644, 1185
BsmI GAATGC 1 cut(s) 912
BsnI GGCC 4 cut(s) 673, 927, 1214, 1312
Bso31I GGTCTC 2 cut(s) 158, 1067
BsoBI CYCGRG 1 cut(s) 360
Bsp119I TTCGAA 1 cut(s) 807
Bsp1286I GDGCHC 2 cut(s) 392, 1022
Bsp143I GATC 2 cut(s) 796, 1240
Bsp1720I GCTNAGC 1 cut(s) 1021
Bsp19I CCATGG 1 cut(s) 1192
BspACI CCGC 1 cut(s) 719
BspANI GGCC 4 cut(s) 673, 927, 1214, 1312
BspCNI CTCAG 5 cut(s) 417, 1034, 1230, 1306, 1314
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 6 cut(s) 206, 391, 585, 1120, 1232, 1291
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 1248
BspT104I TTCGAA 1 cut(s) 807
BspT107I GGYRCC 3 cut(s) 389, 583, 1289
BspTNI GGTCTC 2 cut(s) 158, 1067
BsrFI RCCGGY 2 cut(s) 1091, 1147
BsrI ACTGG 1 cut(s) 853
BssAI RCCGGY 2 cut(s) 1091, 1147
BssECI CCNNGG 6 cut(s) 515, 516, 668, 1062, 1101, 1192
BssMI GATC 2 cut(s) 796, 1240
BssSI CACGAG 1 cut(s) 800
BssT1I CCWWGG 3 cut(s) 668, 1062, 1192
Bst2BI CACGAG 1 cut(s) 800
Bst2UI CCWGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
Bst4CI ACNGT 4 cut(s) 152, 176, 835, 1307
Bst6I CTCTTC 1 cut(s) 392
BstBI TTCGAA 1 cut(s) 807
BstC8I GCNNGC 4 cut(s) 56, 381, 1145, 1149
BstDEI CTNAG 6 cut(s) 404, 732, 1021, 1217, 1314, 1322
BstDSI CCRYGG 1 cut(s) 1192
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 3 cut(s) 37, 1035, 1192
BstFNI CGCG 1 cut(s) 292
BstKTI GATC 2 cut(s) 799, 1243
BstMAI GTCTC 5 cut(s) 131, 158, 407, 1048, 1067
BstMBI GATC 2 cut(s) 796, 1240
BstMWI GCNNNNNNNGC 2 cut(s) 658, 1127
BstNI CCWGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 6 cut(s) 392, 439, 515, 1101, 1125, 1236
BstSFI CTRYAG 1 cut(s) 423
BstSLI GKGCMC 1 cut(s) 392
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 3 cut(s) 400, 422, 1117
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
Bsu36I CCTNAGG 1 cut(s) 1217
BsuRI GGCC 4 cut(s) 673, 927, 1214, 1312
BtgI CCRYGG 1 cut(s) 1192
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 3 cut(s) 37, 1035, 1192
BtsIMutI CAGTG 3 cut(s) 412, 565, 966
Cac8I GCNNGC 4 cut(s) 56, 381, 1145, 1149
CaiI CAGNNNCTG 1 cut(s) 1102
Cfr10I RCCGGY 2 cut(s) 1091, 1147
Cfr13I GGNCC 2 cut(s) 1213, 1231
Csp6I GTAC 3 cut(s) 294, 883, 1303
CspAI ACCGGT 1 cut(s) 1091
CviAII CATG 8 cut(s) 609, 626, 726, 770, 892, 1040, 1193, 1297
CviQI GTAC 3 cut(s) 294, 883, 1303
DdeI CTNAG 6 cut(s) 404, 732, 1021, 1217, 1314, 1322
DpnI GATC 2 cut(s) 798, 1242
DpnII GATC 2 cut(s) 796, 1240
EaeI YGGCCR 1 cut(s) 925
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
EciI GGCGGA 1 cut(s) 734
Eco130I CCWWGG 3 cut(s) 668, 1062, 1192
Eco24I GRGCYC 1 cut(s) 1022
Eco31I GGTCTC 2 cut(s) 158, 1067
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 1231
Eco57I CTGAAG 1 cut(s) 609
Eco81I CCTNAGG 1 cut(s) 1217
Eco88I CYCGRG 1 cut(s) 360
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoO109I RGGNCCY 1 cut(s) 1213
EcoRI GAATTC 1 cut(s) 870
EcoRII CCWGG 6 cut(s) 392, 439, 515, 1101, 1125, 1236
EcoRV GATATC 1 cut(s) 758
EcoT14I CCWWGG 3 cut(s) 668, 1062, 1192
EcoT22I ATGCAT 1 cut(s) 771
EcoT38I GRGCYC 1 cut(s) 1022
ErhI CCWWGG 3 cut(s) 668, 1062, 1192
FaeI CATG 8 cut(s) 612, 629, 729, 773, 895, 1043, 1196, 1300
FalI AAGNNNNNCTT 2 cut(s) 259, 291
FaqI GGGAC 3 cut(s) 154, 644, 1185
FatI CATG 8 cut(s) 608, 625, 725, 769, 891, 1039, 1192, 1296
Fnu4HI GCNGC 3 cut(s) 411, 414, 1131
FokI GGATG 3 cut(s) 44, 1022, 1199
FriOI GRGCYC 1 cut(s) 1022
Fsp4HI GCNGC 3 cut(s) 411, 414, 1131
FspBI CTAG 5 cut(s) 237, 249, 380, 384, 1251
GluI GCNGC 3 cut(s) 411, 414, 1131
GsaI CCCAGC 3 cut(s) 616, 1110, 1359
HaeIII GGCC 4 cut(s) 673, 927, 1214, 1312
HapII CCGG 2 cut(s) 1092, 1148
Hin1II CATG 8 cut(s) 612, 629, 729, 773, 895, 1043, 1196, 1300
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 2 cut(s) 56, 591
HinfI GANTC 6 cut(s) 185, 287, 400, 722, 1056, 1203
HpaI GTTAAC 1 cut(s) 738
HpaII CCGG 2 cut(s) 1092, 1148
HphI GGTGA 8 cut(s) 59, 377, 709, 940, 971, 1082, 1193, 1235
Hpy166II GTNNAC 2 cut(s) 738, 1234
Hpy188I TCNGA 1 cut(s) 1098
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 2 cut(s) 738, 1234
HpyCH4III ACNGT 4 cut(s) 152, 176, 835, 1307
HpyF10VI GCNNNNNNNGC 2 cut(s) 658, 1127
HpyF3I CTNAG 6 cut(s) 404, 732, 1021, 1217, 1314, 1322
Hsp92II CATG 8 cut(s) 612, 629, 729, 773, 895, 1043, 1196, 1300
KroI GCCGGC 1 cut(s) 1147
KroNI GCCGGC 1 cut(s) 1149
KspAI GTTAAC 1 cut(s) 738
Kzo9I GATC 2 cut(s) 796, 1240
LmnI GCTCC 2 cut(s) 172, 1118
Lsp1109I GCAGC 3 cut(s) 400, 422, 1117
LweI GCATC 1 cut(s) 1177
MaeI CTAG 5 cut(s) 237, 249, 380, 384, 1251
MaeIII GTNAC 2 cut(s) 146, 281
MalI GATC 2 cut(s) 798, 1242
MboI GATC 2 cut(s) 796, 1240
MboII GAAGA 3 cut(s) 117, 409, 806
MflI RGATCY 1 cut(s) 796
MhlI GDGCHC 2 cut(s) 392, 1022
MlsI TGGCCA 1 cut(s) 927
MluCI AATT 4 cut(s) 198, 318, 870, 996
MluNI TGGCCA 1 cut(s) 927
MlyI GAGTC 3 cut(s) 409, 1050, 1212
Mox20I TGGCCA 1 cut(s) 927
Mph1103I ATGCAT 1 cut(s) 771
MroNI GCCGGC 1 cut(s) 1147
MroXI GAANNNNTTC 1 cut(s) 112
MscI TGGCCA 1 cut(s) 927
MseI TTAA 3 cut(s) 197, 737, 1372
MslI CAYNNNNRTG 1 cut(s) 695
Msp20I TGGCCA 1 cut(s) 927
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 2 cut(s) 1092, 1148
MspR9I CCNGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
Mva1269I GAATGC 1 cut(s) 912
MvaI CCWGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
MvnI CGCG 1 cut(s) 292
MwoI GCNNNNNNNGC 2 cut(s) 658, 1127
NaeI GCCGGC 1 cut(s) 1149
NcoI CCATGG 1 cut(s) 1192
NdeII GATC 2 cut(s) 796, 1240
NgoMIV GCCGGC 1 cut(s) 1147
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 8 cut(s) 612, 629, 729, 773, 895, 1043, 1196, 1300
NlaIV GGNNCC 6 cut(s) 206, 391, 585, 1120, 1232, 1291
NsiI ATGCAT 1 cut(s) 771
NspI RCATGY 1 cut(s) 612
NspV TTCGAA 1 cut(s) 807
PaeR7I CTCGAG 1 cut(s) 360
PasI CCCWGGG 1 cut(s) 516
PctI GAATGC 1 cut(s) 912
PdiI GCCGGC 1 cut(s) 1149
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 3 cut(s) 185, 287, 722
PinAI ACCGGT 1 cut(s) 1091
PkrI GCNGC 3 cut(s) 412, 415, 1132
PleI GAGTC 3 cut(s) 408, 1050, 1211
PpsI GAGTC 3 cut(s) 408, 1050, 1211
PsiI TTATAA 2 cut(s) 62, 99
Psp6I CCWGG 6 cut(s) 392, 439, 515, 1101, 1125, 1236
PspFI CCCAGC 3 cut(s) 612, 1106, 1355
PspGI CCWGG 6 cut(s) 392, 439, 515, 1101, 1125, 1236
PspN4I GGNNCC 6 cut(s) 206, 391, 585, 1120, 1232, 1291
PspPI GGNCC 2 cut(s) 1213, 1231
PspXI VCTCGAGB 1 cut(s) 360
PstNI CAGNNNCTG 1 cut(s) 1102
PsuI RGATCY 1 cut(s) 796
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 3 cut(s) 295, 884, 1304
RsaNI GTAC 3 cut(s) 294, 883, 1303
RseI CAYNNNNRTG 1 cut(s) 695
SaqAI TTAA 3 cut(s) 197, 737, 1372
SatI GCNGC 3 cut(s) 411, 414, 1131
Sau3AI GATC 2 cut(s) 796, 1240
Sau96I GGNCC 2 cut(s) 1213, 1231
ScaI AGTACT 2 cut(s) 884, 1304
SchI GAGTC 3 cut(s) 409, 1050, 1212
ScrFI CCNGG 6 cut(s) 394, 441, 517, 1103, 1127, 1238
SduI GDGCHC 2 cut(s) 392, 1022
SfaNI GCATC 1 cut(s) 1177
SfcI CTRYAG 1 cut(s) 423
Sfr274I CTCGAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 807
SinI GGWCC 1 cut(s) 1231
SlaI CTCGAG 1 cut(s) 360
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
SpeI ACTAGT 1 cut(s) 1250
Sse9I AATT 4 cut(s) 198, 318, 870, 996
SsiI CCGC 1 cut(s) 719
SspI AATATT 1 cut(s) 454
SspMI CTAG 5 cut(s) 237, 249, 380, 384, 1251
StyD4I CCNGG 6 cut(s) 392, 439, 515, 1101, 1125, 1236
StyI CCWWGG 3 cut(s) 668, 1062, 1192
TaaI ACNGT 4 cut(s) 152, 176, 835, 1307
TaqI TCGA 4 cut(s) 361, 654, 807, 853
TasI AATT 4 cut(s) 198, 318, 870, 996
TatI WGTACW 2 cut(s) 882, 1302
TfiI GAWTC 3 cut(s) 185, 287, 722
Tru1I TTAA 3 cut(s) 197, 737, 1372
Tru9I TTAA 3 cut(s) 197, 737, 1372
TscAI CASTG 3 cut(s) 412, 565, 973
TseI GCWGC 3 cut(s) 410, 413, 1130
TspDTI ATGAA 3 cut(s) 716, 880, 1313
TspRI CASTG 3 cut(s) 412, 565, 973
VpaK11BI GGWCC 1 cut(s) 1231
XagI CCTNNNNNAGG 1 cut(s) 746
XapI RAATTY 1 cut(s) 870
XceI RCATGY 1 cut(s) 612
XcmI CCANNNNNNNNNTGG 1 cut(s) 1279
XhoI CTCGAG 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 5 cut(s) 237, 249, 380, 384, 1251
ZrmI AGTACT 2 cut(s) 884, 1304
Zsp2I ATGCAT 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.