Rh5CG222900

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0019813
Physical Location & Seq
Forward (+)
22712709 .. 22727929
15221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG222900.1

Sequence Viewer

Length: 750 bp
ATGACTTCAATGAGAGCAATAGCATATTCACCATCCCAAAAATGGGACTCCAAATTCCAAACCCATCTCCCAAAACACTCAAACACACCAACCAAATCCCCAATTCAACCCCACAAGACACCCGCCACTTCCCTGCTTCTCCCTAGACTCACCAAAATCCCAAAACCCACTGACCCCAATCACCACCACAACGGCACAACCCGACCACTTGTGGTGGTGGGCTCAGCCAATGCAGACATCCATGTTGAGATTGAGAGACTCCCCAAGGAGGGTGAGACCATCTCAGCCAAAACCGGTCAGACCCTGGCTGGGGGAAAAGGAGCCAACCAGGCTGCCTGTGGTGGCAAGCCGGCTTACCCAACCTACTTTGTGGGCCAGGTGGGTGAGGATGCCCATGGCAAACTGGTCATTGAGGCCCTCAGGGGTGGTGGGGTCCACCTGGATCACCTGACTAGTGTGGCTGCTGCACCCACTGGGCATGCTGTGGTGATGTTGCAGAGTGATGGGCAGAACTCCATTATCATTGTGGGTGGTGCCAACATGAAGTGTTGGCCGGAGAGCCTGAGTGATGAGGATTTGAGGGTTTTAAAGAGTGCTGGGATTGTTTTGCTTCAGAGGGAGATTCCAGAAGTGGTCAACATTCAGGTTGGAAAGGTCGATCAGCTACAATTAATTGTAGAGTTTTTTCTTCATGTGACACGCGCGTGTAGTAAGATAGTGGATCTGGTAATCTACCAGATGTACAAATAA

Protein Analysis

249

Amino Acids

26.85

Weight (kDa)

8.91

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 70 - 217 2.6e-17 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019813)

Species Orthologous Gene IDs
pyrus_communis pycom03g19930
rosa_chinensis RchiOBHm_Chr5g0028931
rosa_laevigata RLG00000033128
rosa_multiflora Rmu_sc0040883.1_g000001 Rmu_sc0040884.1_g000001
rosa_samantha Rh5CG222900 Rh5DG205900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 533
AccII CGCG 2 cut(s) 702, 704
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 450, 729
AcoI YGGCCR 1 cut(s) 551
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 1 cut(s) 596
AfaI GTAC 1 cut(s) 743
AfiI CCNNNNNNNGG 6 cut(s) 42, 43, 268, 269, 309, 310
AgeI ACCGGT 1 cut(s) 293
AgsI TTSAA 2 cut(s) 9, 107
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 4 cut(s) 303, 327, 375, 438
AleI CACNNNNGTG 1 cut(s) 703
AluBI AGCT 1 cut(s) 664
AluI AGCT 1 cut(s) 664
Alw26I GTCTC 2 cut(s) 250, 269
AlwI GGATC 2 cut(s) 450, 729
AlwNI CAGNNNCTG 1 cut(s) 304
AoxI GGCC 3 cut(s) 373, 414, 551
ApeKI GCWGC 3 cut(s) 332, 461, 464
ApoI RAATTY 1 cut(s) 53
AseI ATTAAT 1 cut(s) 671
AsiGI ACCGGT 1 cut(s) 293
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 3 cut(s) 373, 415, 433
AsuHPI GGTGA 7 cut(s) 21, 142, 173, 284, 395, 437, 499
AvaII GGWCC 1 cut(s) 433
AxyI CCTNAGG 1 cut(s) 419
BanI GGYRCC 1 cut(s) 533
BanII GRGCYC 1 cut(s) 224
BbvI GCAGC 3 cut(s) 319, 448, 451
BccI CCATC 4 cut(s) 40, 72, 287, 497
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 4 cut(s) 305, 329, 377, 440
BcoDI GTCTC 2 cut(s) 250, 269
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 2 cut(s) 144, 453
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 3 cut(s) 333, 462, 465
BlpI GCTNAGC 1 cut(s) 223
BlsI GCNGC 3 cut(s) 334, 463, 466
Bme1390I CCNGG 4 cut(s) 305, 329, 377, 440
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 3 cut(s) 373, 415, 433
BmiI GGNNCC 3 cut(s) 322, 434, 535
BmrFI CCNGG 4 cut(s) 305, 329, 377, 440
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 483
BplI GAGNNNNNCTC 2 cut(s) 266, 298
Bpu1102I GCTNAGC 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 264, 303, 394
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 6 cut(s) 42, 43, 268, 269, 309, 310
Bse118I RCCGGY 2 cut(s) 293, 349
Bse1I ACTGG 2 cut(s) 408, 478
Bse21I CCTNAGG 1 cut(s) 419
BseBI CCWGG 4 cut(s) 305, 329, 377, 440
BseDI CCNNGG 3 cut(s) 264, 303, 394
BseGI GGATG 3 cut(s) 32, 237, 394
BseLI CCNNNNNNNGG 6 cut(s) 42, 43, 268, 269, 309, 310
BseMII CTCAG 4 cut(s) 237, 297, 433, 554
BseNI ACTGG 2 cut(s) 408, 478
BseXI GCAGC 3 cut(s) 319, 448, 451
BseYI CCCAGC 2 cut(s) 308, 596
BsgI GTGCAG 1 cut(s) 450
Bsh1236I CGCG 2 cut(s) 702, 704
BshFI GGCC 3 cut(s) 375, 416, 553
BshNI GGYRCC 1 cut(s) 533
BshTI ACCGGT 1 cut(s) 293
BsiSI CCGG 3 cut(s) 294, 350, 554
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 6 cut(s) 42, 43, 268, 269, 309, 310
BsmAI GTCTC 2 cut(s) 250, 269
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 3 cut(s) 375, 416, 553
Bso31I GGTCTC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 3 cut(s) 442, 658, 721
Bsp1720I GCTNAGC 1 cut(s) 223
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 1 cut(s) 123
BspANI GGCC 3 cut(s) 375, 416, 553
BspCNI CTCAG 4 cut(s) 236, 296, 432, 555
BspFNI CGCG 2 cut(s) 702, 704
BspLI GGNNCC 3 cut(s) 322, 434, 535
BspPI GGATC 2 cut(s) 450, 729
BspT107I GGYRCC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 269
BsrFI RCCGGY 2 cut(s) 293, 349
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 2 cut(s) 408, 478
BssAI RCCGGY 2 cut(s) 293, 349
BssECI CCNNGG 3 cut(s) 264, 303, 394
BssMI GATC 3 cut(s) 442, 658, 721
BssT1I CCWWGG 2 cut(s) 264, 394
Bst2UI CCWGG 4 cut(s) 305, 329, 377, 440
BstAUI TGTACA 1 cut(s) 741
BstC8I GCNNGC 3 cut(s) 347, 351, 480
BstDEI CTNAG 4 cut(s) 223, 283, 419, 563
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 3 cut(s) 32, 237, 394
BstFNI CGCG 2 cut(s) 702, 704
BstHHI GCGC 1 cut(s) 704
BstKTI GATC 3 cut(s) 445, 661, 724
BstMAI GTCTC 2 cut(s) 250, 269
BstMBI GATC 3 cut(s) 442, 658, 721
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 4 cut(s) 305, 329, 377, 440
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 4 cut(s) 303, 327, 375, 438
BstUI CGCG 2 cut(s) 702, 704
BstV1I GCAGC 3 cut(s) 319, 448, 451
BstX2I RGATCY 1 cut(s) 721
BstYI RGATCY 1 cut(s) 721
Bsu36I CCTNAGG 1 cut(s) 419
BsuRI GGCC 3 cut(s) 375, 416, 553
BtgI CCRYGG 1 cut(s) 394
BtsCI GGATG 3 cut(s) 32, 237, 394
BtsIMutI CAGTG 2 cut(s) 168, 471
Cac8I GCNNGC 3 cut(s) 347, 351, 480
CaiI CAGNNNCTG 1 cut(s) 304
CfoI GCGC 1 cut(s) 704
Cfr10I RCCGGY 2 cut(s) 293, 349
Cfr13I GGNCC 3 cut(s) 373, 415, 433
Csp6I GTAC 1 cut(s) 742
CspAI ACCGGT 1 cut(s) 293
CviAII CATG 5 cut(s) 242, 395, 479, 541, 692
CviQI GTAC 1 cut(s) 742
DdeI CTNAG 4 cut(s) 223, 283, 419, 563
DpnI GATC 3 cut(s) 444, 660, 723
DpnII GATC 3 cut(s) 442, 658, 721
DraI TTTAAA 1 cut(s) 588
EaeI YGGCCR 1 cut(s) 551
Eco130I CCWWGG 2 cut(s) 264, 394
Eco24I GRGCYC 1 cut(s) 224
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 596
Eco81I CCTNAGG 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 4 cut(s) 303, 327, 375, 438
EcoT14I CCWWGG 2 cut(s) 264, 394
EcoT38I GRGCYC 1 cut(s) 224
ErhI CCWWGG 2 cut(s) 264, 394
FaeI CATG 5 cut(s) 245, 398, 482, 544, 695
FaiI YATR 6 cut(s) 25, 243, 396, 480, 542, 693
FaqI GGGAC 1 cut(s) 59
FatI CATG 5 cut(s) 241, 394, 478, 540, 691
FauI CCCGC 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 333, 462, 465
FokI GGATG 3 cut(s) 19, 224, 401
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 3 cut(s) 333, 462, 465
FspBI CTAG 2 cut(s) 144, 453
GlaI GCGC 1 cut(s) 703
GluI GCNGC 3 cut(s) 333, 462, 465
GsaI CCCAGC 2 cut(s) 312, 600
HaeIII GGCC 3 cut(s) 375, 416, 553
HapII CCGG 3 cut(s) 294, 350, 554
HhaI GCGC 1 cut(s) 704
Hin1II CATG 5 cut(s) 245, 398, 482, 544, 695
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HinfI GANTC 4 cut(s) 47, 147, 258, 622
HpaII CCGG 3 cut(s) 294, 350, 554
HphI GGTGA 7 cut(s) 21, 142, 173, 284, 395, 437, 499
Hpy166II GTNNAC 2 cut(s) 436, 637
Hpy188I TCNGA 2 cut(s) 300, 615
Hpy188III TCNNGA 1 cut(s) 626
Hpy8I GTNNAC 2 cut(s) 436, 637
HpyCH4V TGCA 3 cut(s) 233, 467, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 223, 283, 419, 563
Hsp92II CATG 5 cut(s) 245, 398, 482, 544, 695
HspAI GCGC 1 cut(s) 702
KroI GCCGGC 1 cut(s) 349
KroNI GCCGGC 1 cut(s) 351
Kzo9I GATC 3 cut(s) 442, 658, 721
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 3 cut(s) 319, 448, 451
LweI GCATC 1 cut(s) 379
MaeI CTAG 2 cut(s) 144, 453
MaeIII GTNAC 1 cut(s) 694
MalI GATC 3 cut(s) 444, 660, 723
MboI GATC 3 cut(s) 442, 658, 721
MboII GAAGA 1 cut(s) 680
MflI RGATCY 1 cut(s) 721
MhlI GDGCHC 1 cut(s) 224
MluCI AATT 4 cut(s) 53, 102, 668, 672
MlyI GAGTC 3 cut(s) 41, 141, 252
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 7 cut(s) 262, 379, 406, 428, 565, 573, 609
MroNI GCCGGC 1 cut(s) 349
MseI TTAA 2 cut(s) 587, 671
MslI CAYNNNNRTG 1 cut(s) 703
MspI CCGG 3 cut(s) 294, 350, 554
MspR9I CCNGG 4 cut(s) 305, 329, 377, 440
MvaI CCWGG 4 cut(s) 305, 329, 377, 440
MvnI CGCG 2 cut(s) 702, 704
MwoI GCNNNNNNNGC 1 cut(s) 329
NaeI GCCGGC 1 cut(s) 351
NcoI CCATGG 1 cut(s) 394
NdeII GATC 3 cut(s) 442, 658, 721
NgoMIV GCCGGC 1 cut(s) 349
NlaIII CATG 5 cut(s) 245, 398, 482, 544, 695
NlaIV GGNNCC 3 cut(s) 322, 434, 535
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 1 cut(s) 482
OliI CACNNNNGTG 1 cut(s) 703
PaeI GCATGC 1 cut(s) 482
PdiI GCCGGC 1 cut(s) 351
PfeI GAWTC 1 cut(s) 622
PinAI ACCGGT 1 cut(s) 293
PkrI GCNGC 3 cut(s) 334, 463, 466
PleI GAGTC 3 cut(s) 41, 141, 252
PpsI GAGTC 3 cut(s) 41, 141, 252
PshBI ATTAAT 1 cut(s) 671
Psp6I CCWGG 4 cut(s) 303, 327, 375, 438
PspFI CCCAGC 2 cut(s) 308, 596
PspGI CCWGG 4 cut(s) 303, 327, 375, 438
PspN4I GGNNCC 3 cut(s) 322, 434, 535
PspPI GGNCC 3 cut(s) 373, 415, 433
PstNI CAGNNNCTG 1 cut(s) 304
PsuI RGATCY 1 cut(s) 721
RsaI GTAC 1 cut(s) 743
RsaNI GTAC 1 cut(s) 742
RseI CAYNNNNRTG 1 cut(s) 703
SaqAI TTAA 2 cut(s) 587, 671
SatI GCNGC 3 cut(s) 333, 462, 465
Sau3AI GATC 3 cut(s) 442, 658, 721
Sau96I GGNCC 3 cut(s) 373, 415, 433
SchI GAGTC 3 cut(s) 41, 141, 252
ScrFI CCNGG 4 cut(s) 305, 329, 377, 440
SduI GDGCHC 1 cut(s) 224
SetI ASST 7 cut(s) 365, 381, 441, 450, 648, 657, 666
SfaNI GCATC 1 cut(s) 379
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 703
SpeI ACTAGT 1 cut(s) 452
SphI GCATGC 1 cut(s) 482
Sse9I AATT 4 cut(s) 53, 102, 668, 672
SsiI CCGC 1 cut(s) 123
SspMI CTAG 2 cut(s) 144, 453
StyD4I CCNGG 4 cut(s) 303, 327, 375, 438
StyI CCWWGG 2 cut(s) 264, 394
TaqI TCGA 1 cut(s) 657
TasI AATT 4 cut(s) 53, 102, 668, 672
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 2 cut(s) 587, 671
Tru9I TTAA 2 cut(s) 587, 671
TscAI CASTG 2 cut(s) 175, 478
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 3 cut(s) 332, 461, 464
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 2 cut(s) 557, 680
TspRI CASTG 2 cut(s) 175, 478
VpaK11BI GGWCC 1 cut(s) 433
VspI ATTAAT 1 cut(s) 671
XapI RAATTY 1 cut(s) 53
XceI RCATGY 1 cut(s) 482
XcmI CCANNNNNNNNNTGG 3 cut(s) 39, 335, 523
XspI CTAG 2 cut(s) 144, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.