RLG00000033610

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0021181
Physical Location & Seq
Forward (+)
29600675 .. 29602268
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033610

Sequence Viewer

Length: 396 bp
ATGAAGGTGGCCACTAAAATTTACAGGGACCCGTTTTTGTCACGAACGAAGTTCGATGAACATTCAAACTTTTCAGAAGCAGCGAACAATAGTGTAGAAAAAAAAGTGCTGCCAATTTTCTATGGAATCAATCCCTCTGATGTACGACATCAAACGGGAAGTTTTGCAGATGCGTTTGCAAAACTTGAAGAAACGTACAAGGAGAAGCCGGGAAAAGTAGAAAGGTGGAGAAGTGCTTTAAGGAAATTAGCAGATCTTGCCGGGTGGCATTCAAAGGACCGGATTCAGTTGTCGAGTCCAAGGTTCACAATGAGCCTAACACTCAGTCATCTTCTCGAAGACAACCTAAACCTCTTCAATCTAGTGTTAAAGCACAACATAAGTTTCTCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

132

Amino Acids

15.07

Weight (kDa)

9.56

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 93 1.2e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021181)

Species Orthologous Gene IDs
malus_domestica MD04G1034900.v1.1
pyrus_communis pycom04g02970
rosa_laevigata RLG00000033610
rosa_multiflora Rmu_sc0006084.1_g000011 Rmu_sc0006803.1_g000037

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 18
AfaI GTAC 2 cut(s) 144, 197
AgsI TTSAA 4 cut(s) 66, 188, 273, 358
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 2 cut(s) 80, 109
ApoI RAATTY 1 cut(s) 18
AspS9I GGNCC 2 cut(s) 28, 277
AsuC2I CCSGG 2 cut(s) 210, 262
AvaII GGWCC 2 cut(s) 28, 277
BalI TGGCCA 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 92, 96
BcnI CCSGG 2 cut(s) 210, 262
BfaI CTAG 2 cut(s) 362, 394
BglII AGATCT 1 cut(s) 253
BisI GCNGC 2 cut(s) 81, 110
BlsI GCNGC 2 cut(s) 82, 111
Bme1390I CCNGG 2 cut(s) 210, 262
Bme18I GGWCC 2 cut(s) 28, 277
BmgT120I GGNCC 2 cut(s) 28, 277
BmiI GGNNCC 2 cut(s) 29, 30
BmrFI CCNGG 2 cut(s) 210, 262
BmsI GCATC 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 345
BpuMI CCSGG 2 cut(s) 210, 262
BsaJI CCNNGG 1 cut(s) 299
BsaWI WCCGGW 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 299
BseGI GGATG 1 cut(s) 389
BseMII CTCAG 1 cut(s) 337
BseXI GCAGC 2 cut(s) 92, 96
BshFI GGCC 1 cut(s) 11
BsiSI CCGG 3 cut(s) 209, 261, 280
BslFI GGGAC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 41
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 1 cut(s) 11
Bsp143I GATC 1 cut(s) 253
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 336
BspLI GGNNCC 2 cut(s) 29, 30
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 359
BstAPI GCANNNNNTGC 1 cut(s) 257
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 389
BstKTI GATC 1 cut(s) 256
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstSCI CCNGG 2 cut(s) 208, 260
BstV1I GCAGC 2 cut(s) 92, 96
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 11
BtsCI GGATG 1 cut(s) 389
Cfr13I GGNCC 2 cut(s) 28, 277
Csp6I GTAC 2 cut(s) 143, 196
CviJI RGCY 3 cut(s) 11, 208, 315
CviKI_1 RGCY 3 cut(s) 11, 208, 315
CviQI GTAC 2 cut(s) 143, 196
DdeI CTNAG 1 cut(s) 323
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 299
Eco47I GGWCC 2 cut(s) 28, 277
EcoO109I RGGNCCY 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 299
FaiI YATR 2 cut(s) 123, 380
FaqI GGGAC 1 cut(s) 41
Fnu4HI GCNGC 2 cut(s) 81, 110
FokI GGATG 1 cut(s) 376
Fsp4HI GCNGC 2 cut(s) 81, 110
FspBI CTAG 2 cut(s) 362, 394
GluI GCNGC 2 cut(s) 81, 110
HaeIII GGCC 1 cut(s) 11
HapII CCGG 3 cut(s) 209, 261, 280
HinfI GANTC 3 cut(s) 126, 283, 295
HpaII CCGG 3 cut(s) 209, 261, 280
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 2 cut(s) 76, 139
Hpy188III TCNNGA 2 cut(s) 42, 335
Hpy8I GTNNAC 1 cut(s) 306
HpyCH4IV ACGT 1 cut(s) 194
HpyCH4V TGCA 2 cut(s) 167, 179
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 1 cut(s) 194
KflI GGGWCCC 1 cut(s) 28
Kzo9I GATC 1 cut(s) 253
LpnPI CCDG 4 cut(s) 10, 222, 274, 293
Lsp1109I GCAGC 2 cut(s) 92, 96
LweI GCATC 1 cut(s) 160
MaeI CTAG 2 cut(s) 362, 394
MaeII ACGT 1 cut(s) 194
MaeIII GTNAC 1 cut(s) 39
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 4 cut(s) 200, 323, 346, 350
MflI RGATCY 1 cut(s) 253
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 3 cut(s) 18, 114, 245
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 2 cut(s) 145, 362
Mox20I TGGCCA 1 cut(s) 11
MscI TGGCCA 1 cut(s) 11
MseI TTAA 2 cut(s) 239, 368
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 3 cut(s) 209, 261, 280
MspR9I CCNGG 2 cut(s) 210, 262
Mva1269I GAATGC 1 cut(s) 268
MwoI GCNNNNNNNGC 1 cut(s) 257
NciI CCSGG 2 cut(s) 210, 262
NdeII GATC 1 cut(s) 253
NlaIV GGNNCC 2 cut(s) 29, 30
NmuCI GTSAC 1 cut(s) 39
PctI GAATGC 1 cut(s) 268
PfeI GAWTC 2 cut(s) 126, 283
PkrI GCNGC 2 cut(s) 82, 111
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PpuMI RGGWCCY 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 29, 30
PspPI GGNCC 2 cut(s) 28, 277
PspPPI RGGWCCY 1 cut(s) 28
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 2 cut(s) 144, 197
RsaNI GTAC 2 cut(s) 143, 196
SaqAI TTAA 2 cut(s) 239, 368
SatI GCNGC 2 cut(s) 81, 110
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 2 cut(s) 28, 277
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 210, 262
SetI ASST 6 cut(s) 9, 197, 227, 305, 348, 354
SfaNI GCATC 1 cut(s) 160
SinI GGWCC 2 cut(s) 28, 277
Sse9I AATT 3 cut(s) 18, 114, 245
SspMI CTAG 2 cut(s) 362, 394
StyD4I CCNGG 2 cut(s) 208, 260
StyI CCWWGG 1 cut(s) 299
TaiI ACGT 1 cut(s) 197
TaqI TCGA 3 cut(s) 54, 293, 336
TasI AATT 3 cut(s) 18, 114, 245
TfiI GAWTC 2 cut(s) 126, 283
Tru1I TTAA 2 cut(s) 239, 368
Tru9I TTAA 2 cut(s) 239, 368
TseFI GTSAC 1 cut(s) 39
TseI GCWGC 2 cut(s) 80, 109
Tsp45I GTSAC 1 cut(s) 39
TspDTI ATGAA 2 cut(s) 17, 72
VpaK11BI GGWCC 2 cut(s) 28, 277
XapI RAATTY 1 cut(s) 18
XspI CTAG 2 cut(s) 362, 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.