RLG00000035109

histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019124
Physical Location & Seq
Reverse (-)
61038136 .. 61038552
417 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035109

Sequence Viewer

Length: 417 bp
ATGGCACCAAAGGCAGAGAAGAAGCCAGCCGGGAAGAAACCGGTGGAGGAGAAGCTAGCCGAGAAAGCTCCAGCTGAGAAGAAGCTTTCGGAGTGGAAGGACGCTGCCGAGAAGAAGAAGAAGCGTCTGAAGAAGAGCGTGGAAACCTACAAGACCTACATCTTCAAGGTGCTGAAGCAGGTGCACCCTGACATTGGGATCTCGAGCAAGGCCATGGGGATCATGAACAGTTTCATCAATGACATATTAGAGAAGCTGGCTGCCGAGTCTTCTAAGCTTGCGAGGTACAATAAGCAGATGACGATCACTTCGAGGGAGATTCAGACTGCGGTGAGGTTGGTGCTTCCTGGTGAGCTTGCCAAGCATGCTGTTTCTGAGGGGACTAAGGCGGTGACTAAGTTTACTAGTACTAATTAG

Protein Analysis

139

Amino Acids

15.37

Weight (kDa)

9.91

Isoelectric Point (pI)

25.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 32 - 113 6.9e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 67 - 135 1.3e-07 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019124)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0056591
rosa_laevigata RLG00000035109
rosa_multiflora Rmu_co8014260.1_g000001 Rmu_sc0004101.1_g000046
rosa_samantha Rh5AG371300 Rh5BG382700 Rh5CG406000
rosa_wichuraiana Rw5G034780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 2 cut(s) 329, 389
AclWI GGATC 2 cut(s) 206, 227
AcuI CTGAAG 2 cut(s) 149, 194
AfaI GTAC 2 cut(s) 287, 409
AfiI CCNNNNNNNGG 1 cut(s) 194
AgeI ACCGGT 1 cut(s) 40
AgsI TTSAA 1 cut(s) 166
AhlI ACTAGT 1 cut(s) 404
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 7 cut(s) 55, 68, 74, 85, 256, 277, 355
AluI AGCT 7 cut(s) 55, 68, 74, 85, 256, 277, 355
Alw21I GWGCWC 1 cut(s) 186
Alw44I GTGCAC 1 cut(s) 182
AlwI GGATC 2 cut(s) 206, 227
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 1 cut(s) 210
ApaLI GTGCAC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 104, 260
ArsI GACNNNNNNTTYG 2 cut(s) 292, 324
AsiGI ACCGGT 1 cut(s) 40
Asp700I GAANNNNTTC 1 cut(s) 230
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 3 cut(s) 343, 362, 403
AsuNHI GCTAGC 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 91, 247
BciT130I CCWGG 1 cut(s) 348
BcnI CCSGG 1 cut(s) 31
BcuI ACTAGT 1 cut(s) 404
BfaI CTAG 2 cut(s) 56, 405
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 2 cut(s) 105, 261
BlsI GCNGC 2 cut(s) 106, 262
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 2 cut(s) 31, 348
BmeT110I CYCGRG 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 31, 348
BmtI GCTAGC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 54
BpuMI CCSGG 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 213
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 2 cut(s) 66, 366
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 2 cut(s) 91, 247
BshFI GGCC 1 cut(s) 212
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 2 cut(s) 30, 41
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 198, 219, 303
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 2 cut(s) 329, 389
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 2 cut(s) 67, 367
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 169
BspOI GCTAGC 1 cut(s) 59
BspPI GGATC 2 cut(s) 206, 227
BspQI GCTCTTC 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 40
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 3 cut(s) 198, 219, 303
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 128
BstC8I GCNNGC 6 cut(s) 27, 57, 258, 279, 357, 366
BstDEI CTNAG 5 cut(s) 75, 273, 375, 384, 396
BstDSI CCRYGG 1 cut(s) 213
BstKTI GATC 3 cut(s) 201, 222, 306
BstMBI GATC 3 cut(s) 198, 219, 303
BstMWI GCNNNNNNNGC 4 cut(s) 11, 65, 361, 365
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 29, 346
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 91, 247
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BsuRI GGCC 1 cut(s) 212
BtgI CCRYGG 1 cut(s) 213
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 6 cut(s) 27, 57, 258, 279, 357, 366
CciI TCATGA 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 40
CseI GACGC 2 cut(s) 110, 113
Csp6I GTAC 2 cut(s) 286, 408
CspAI ACCGGT 1 cut(s) 40
CviAII CATG 3 cut(s) 214, 223, 365
CviQI GTAC 2 cut(s) 286, 408
DdeI CTNAG 5 cut(s) 75, 273, 375, 384, 396
DpnI GATC 3 cut(s) 200, 221, 305
DpnII GATC 3 cut(s) 198, 219, 303
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 213
Eco57I CTGAAG 2 cut(s) 149, 194
Eco88I CYCGRG 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 3 cut(s) 217, 226, 368
FaiI YATR 4 cut(s) 215, 224, 245, 366
FaqI GGGAC 1 cut(s) 394
FatI CATG 3 cut(s) 213, 222, 364
Fnu4HI GCNGC 2 cut(s) 105, 261
Fsp4HI GCNGC 2 cut(s) 105, 261
FspBI CTAG 2 cut(s) 56, 405
GluI GCNGC 2 cut(s) 105, 261
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 1 cut(s) 212
HapII CCGG 2 cut(s) 30, 41
HgaI GACGC 2 cut(s) 110, 113
Hin1II CATG 3 cut(s) 217, 226, 368
HindIII AAGCTT 2 cut(s) 83, 275
HinfI GANTC 2 cut(s) 266, 319
HpaII CCGG 2 cut(s) 30, 41
HphI GGTGA 3 cut(s) 343, 362, 403
Hpy166II GTNNAC 2 cut(s) 184, 402
Hpy188I TCNGA 4 cut(s) 91, 129, 324, 376
Hpy188III TCNNGA 2 cut(s) 202, 223
Hpy8I GTNNAC 2 cut(s) 184, 402
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4V TGCA 1 cut(s) 184
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 65, 361, 365
HpyF3I CTNAG 5 cut(s) 75, 273, 375, 384, 396
Hsp92II CATG 3 cut(s) 217, 226, 368
Kzo9I GATC 3 cut(s) 198, 219, 303
LguI GCTCTTC 1 cut(s) 128
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 9 cut(s) 39, 43, 54, 84, 164, 201, 242, 333, 360
Lsp1109I GCAGC 2 cut(s) 91, 247
MaeI CTAG 2 cut(s) 56, 405
MaeIII GTNAC 1 cut(s) 391
MalI GATC 3 cut(s) 200, 221, 305
MboI GATC 3 cut(s) 198, 219, 303
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 1 cut(s) 412
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 5 cut(s) 40, 276, 306, 327, 370
MroXI GAANNNNTTC 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 2 cut(s) 30, 41
MspR9I CCNGG 2 cut(s) 31, 348
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 4 cut(s) 11, 65, 361, 365
NciI CCSGG 1 cut(s) 31
NcoI CCATGG 1 cut(s) 213
NdeII GATC 3 cut(s) 198, 219, 303
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 3 cut(s) 217, 226, 368
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 3 cut(s) 85, 133, 289
NmuCI GTSAC 1 cut(s) 391
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 202
PagI TCATGA 1 cut(s) 222
PaqCI CACCTGC 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 319
PinAI ACCGGT 1 cut(s) 40
PkrI GCNGC 2 cut(s) 106, 262
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 198
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 2 cut(s) 287, 409
RsaNI GTAC 2 cut(s) 286, 408
SapI GCTCTTC 1 cut(s) 128
SatI GCNGC 2 cut(s) 105, 261
Sau3AI GATC 3 cut(s) 198, 219, 303
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 31, 348
SduI GDGCHC 1 cut(s) 186
Sfr274I CTCGAG 1 cut(s) 202
SlaI CTCGAG 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
SpeI ACTAGT 1 cut(s) 404
SphI GCATGC 1 cut(s) 368
Sse9I AATT 1 cut(s) 412
SsiI CCGC 2 cut(s) 329, 389
SspMI CTAG 2 cut(s) 56, 405
StyD4I CCNGG 2 cut(s) 29, 346
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 230
TaqI TCGA 2 cut(s) 203, 311
TasI AATT 1 cut(s) 412
TatI WGTACW 1 cut(s) 407
TfiI GAWTC 1 cut(s) 319
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 2 cut(s) 104, 260
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 223, 239
VneI GTGCAC 1 cut(s) 182
XceI RCATGY 1 cut(s) 368
XhoI CTCGAG 1 cut(s) 202
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 2 cut(s) 56, 405
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.