Rw5G034780

Histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0019124
Physical Location & Seq
Reverse (-)
60018427 .. 60019043
617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034780.1

Sequence Viewer

Length: 420 bp
ATGGCACCAAAGGCAGAGAAGAAGCCGGCCGGGAAGAAACCGGTGGAGGAGAAGCTAGCCGAGAAAGCTCCCGCTGAGAAGAAGCTTCCGGAGTCGAAGGACGCTGCCGAGAAGAAGAAGAAGAAGCGTCCGAAGAAGAGCGTGGAAACCTACAAGACCTACATCTTCAAGGTGCTGAAGCAGGTGCACCCTGACATTGGGATCTCGAGCAAGGCCATGGGGATCATGAACAGTTTCATCAATGACATATTTGAGAAGCTGGCTGCCGAGTCTTCCAAGCTTGCGAGGTACAATAAGCAAATGACGATCACTTCGAGGGAGATTCAGACTGCGGTGAGGTTGGTGCTTCCTGGTGAGCTTGCCAAGCACGCTGTTTCTGAGGGGACTAAGGCGGTGACTAAGCTTACTAGTTCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

139

Amino Acids

15.38

Weight (kDa)

9.94

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 33 - 114 3.9e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 68 - 134 2e-06 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019124)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0056591
rosa_laevigata RLG00000035109
rosa_multiflora Rmu_co8014260.1_g000001 Rmu_sc0004101.1_g000046
rosa_samantha Rh5AG371300 Rh5BG382700 Rh5CG406000
rosa_wichuraiana Rw5G034780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 4
AccIII TCCGGA 1 cut(s) 88
AciI CCGC 3 cut(s) 72, 332, 392
AclWI GGATC 2 cut(s) 209, 230
AcoI YGGCCR 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 40
AgsI TTSAA 1 cut(s) 169
AhlI ACTAGT 1 cut(s) 407
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 7 cut(s) 55, 68, 85, 259, 280, 358, 403
AluI AGCT 7 cut(s) 55, 68, 85, 259, 280, 358, 403
Alw21I GWGCWC 1 cut(s) 189
Alw44I GTGCAC 1 cut(s) 185
AlwI GGATC 2 cut(s) 209, 230
Ama87I CYCGRG 1 cut(s) 205
Aor13HI TCCGGA 1 cut(s) 88
AoxI GGCC 2 cut(s) 27, 213
ApaLI GTGCAC 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 104, 263
ArsI GACNNNNNNTTYG 2 cut(s) 295, 327
AsiGI ACCGGT 1 cut(s) 40
Asp700I GAANNNNTTC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 3 cut(s) 346, 365, 406
AsuNHI GCTAGC 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 205
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 91, 250
BciT130I CCWGG 1 cut(s) 351
BcnI CCSGG 1 cut(s) 31
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 2 cut(s) 56, 408
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 2 cut(s) 105, 264
BlsI GCNGC 2 cut(s) 106, 265
Bme1390I CCNGG 2 cut(s) 31, 351
BmeT110I CYCGRG 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 31, 351
BmtI GCTAGC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 2 cut(s) 40, 88
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse118I RCCGGY 2 cut(s) 25, 40
BseAI TCCGGA 1 cut(s) 88
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMII CTCAG 2 cut(s) 66, 369
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 1 cut(s) 189
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 91, 250
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 2 cut(s) 29, 215
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 4 cut(s) 26, 30, 41, 89
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 2 cut(s) 29, 215
BsoBI CYCGRG 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 189
Bsp13I TCCGGA 1 cut(s) 88
Bsp143I GATC 3 cut(s) 201, 222, 306
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 3 cut(s) 72, 332, 392
BspANI GGCC 2 cut(s) 29, 215
BspCNI CTCAG 2 cut(s) 67, 370
BspEI TCCGGA 1 cut(s) 88
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 172
BspOI GCTAGC 1 cut(s) 59
BspPI GGATC 2 cut(s) 209, 230
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 2 cut(s) 25, 40
BssAI RCCGGY 2 cut(s) 25, 40
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 3 cut(s) 201, 222, 306
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 131
BstC8I GCNNGC 6 cut(s) 27, 57, 261, 282, 360, 369
BstDEI CTNAG 4 cut(s) 75, 378, 387, 399
BstDSI CCRYGG 1 cut(s) 216
BstKTI GATC 3 cut(s) 204, 225, 309
BstMBI GATC 3 cut(s) 201, 222, 306
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 4 cut(s) 11, 65, 364, 368
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 2 cut(s) 29, 349
BstSLI GKGCMC 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 91, 250
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 2 cut(s) 29, 215
BtgI CCRYGG 1 cut(s) 216
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 6 cut(s) 27, 57, 261, 282, 360, 369
CciI TCATGA 1 cut(s) 225
Cfr10I RCCGGY 2 cut(s) 25, 40
CseI GACGC 2 cut(s) 110, 116
Csp6I GTAC 1 cut(s) 289
CspAI ACCGGT 1 cut(s) 40
CviAII CATG 2 cut(s) 217, 226
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 4 cut(s) 75, 378, 387, 399
DpnI GATC 3 cut(s) 203, 224, 308
DpnII GATC 3 cut(s) 201, 222, 306
EaeI YGGCCR 1 cut(s) 27
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 216
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 220, 229
FaiI YATR 3 cut(s) 218, 227, 248
FaqI GGGAC 1 cut(s) 397
FatI CATG 2 cut(s) 216, 225
FauI CCCGC 1 cut(s) 79
Fnu4HI GCNGC 2 cut(s) 105, 264
Fsp4HI GCNGC 2 cut(s) 105, 264
FspBI CTAG 2 cut(s) 56, 408
GluI GCNGC 2 cut(s) 105, 264
HaeIII GGCC 2 cut(s) 29, 215
HapII CCGG 4 cut(s) 26, 30, 41, 89
HgaI GACGC 2 cut(s) 110, 116
Hin1II CATG 2 cut(s) 220, 229
HindIII AAGCTT 3 cut(s) 83, 278, 401
HinfI GANTC 3 cut(s) 92, 269, 322
HpaII CCGG 4 cut(s) 26, 30, 41, 89
HphI GGTGA 3 cut(s) 346, 365, 406
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 3 cut(s) 132, 327, 379
Hpy188III TCNNGA 3 cut(s) 89, 205, 226
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4V TGCA 1 cut(s) 187
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 65, 364, 368
HpyF3I CTNAG 4 cut(s) 75, 378, 387, 399
Hsp92II CATG 2 cut(s) 220, 229
Kpn2I TCCGGA 1 cut(s) 88
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 3 cut(s) 201, 222, 306
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 9 cut(s) 39, 43, 54, 102, 167, 204, 245, 336, 363
Lsp1109I GCAGC 2 cut(s) 91, 250
MaeI CTAG 2 cut(s) 56, 408
MaeIII GTNAC 1 cut(s) 394
MalI GATC 3 cut(s) 203, 224, 308
MboI GATC 3 cut(s) 201, 222, 306
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 1 cut(s) 415
MlyI GAGTC 2 cut(s) 101, 278
MnlI CCTC 5 cut(s) 40, 279, 309, 330, 373
MroI TCCGGA 1 cut(s) 88
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 4 cut(s) 26, 30, 41, 89
MspR9I CCNGG 2 cut(s) 31, 351
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 4 cut(s) 11, 65, 364, 368
NaeI GCCGGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 31
NcoI CCATGG 1 cut(s) 216
NdeII GATC 3 cut(s) 201, 222, 306
NgoMIV GCCGGC 1 cut(s) 25
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 2 cut(s) 220, 229
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 3 cut(s) 85, 133, 292
NmuCI GTSAC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 205
PagI TCATGA 1 cut(s) 225
PaqCI CACCTGC 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 1 cut(s) 322
PinAI ACCGGT 1 cut(s) 40
PkrI GCNGC 2 cut(s) 106, 265
PleI GAGTC 2 cut(s) 100, 277
PpsI GAGTC 2 cut(s) 100, 277
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 201
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SapI GCTCTTC 1 cut(s) 131
SatI GCNGC 2 cut(s) 105, 264
Sau3AI GATC 3 cut(s) 201, 222, 306
SchI GAGTC 2 cut(s) 101, 278
ScrFI CCNGG 2 cut(s) 31, 351
SduI GDGCHC 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 205
SlaI CTCGAG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
SpeI ACTAGT 1 cut(s) 407
Sse9I AATT 1 cut(s) 415
SsiI CCGC 3 cut(s) 72, 332, 392
SspMI CTAG 2 cut(s) 56, 408
StyD4I CCNGG 2 cut(s) 29, 349
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 233
TaqI TCGA 3 cut(s) 95, 206, 314
TasI AATT 1 cut(s) 415
TfiI GAWTC 1 cut(s) 322
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 2 cut(s) 104, 263
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 2 cut(s) 226, 242
VneI GTGCAC 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 2 cut(s) 56, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.