Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0008741

Physical Location & Seq

Genomic Coordinates Reverse (-)

62404238 .. 62409869

Gene Structure 5632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000035180

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGAAGAAATACAGTCACAGTCAGATAATTATAGGTCTTCTTCATCGTCAGCAAGCAGTCCAGCAAGTAGGGTGCCCTCAAGTAATTTTTTCTACTTGCGCAAACCTGGTTCAGTCAGACAACCAATCTCATTTGAAGACTCACCTGAATGGGAGGATACAGAGGTTGATGTTAGGGTGGAAGAAGGAGGTGACTCTATCAACATTGCAACCACTCCGGTCTCCCCATCGCTTTCAAAGCTCAATAGTGGGTCCTTGCCATCCCCACCATTACCAGAGGGTGCAACTGTAGTGAGAAAGATTGCAGGGGCTTCAATTGTGTGGAAGGACTTGACTGTTACAATAAAAGGAAAAAGAAAGTACTCTGAGAGGGTTGTGAAGAGTTCAAATGGTTATGCATTGCCAGGAACTATAACAGTAATTATGGGTCCTGCTAAATCGGGGAAGTCCACACTACTAAGAGCACTTGCAGGAAGACTGCCTCAATCAGCGAAAACGTATGGTGAAGTGTTTGTAAACGGTGCGAAATCATCGATGCCATATGGTTCATATGGTTTTGTTAAGAGGGAAATCAATTTAATTGGATCTCTCACTGTTCGGGAGTTTCTGTATTATTCGGCACTCCTTCAGCTTCCTGGTTTCTTTTGTCAGAAAAAGAGTGTAGTAGAGGATGCTATTCATGCCATGTCACTGGGTGATTATGCAAACAGATTGATAGGTGGCCACTGTTATATGAAAGGCCTTCCTAATGGAGAGAGAAGGCGCGTTAGCATTGCCCGAGAGCTTGTGATGAGGCCTCATGTTTTATTCATAGATGAGCCTCTTTATCACCTTGATAGTGTCTCTGCACTTCTGATGATGGTTACATTGAAGAAGCTTGCAAGTACAGGTTGCACTCTTATATTTACCATTTACCAGAGCAGCACAGAAGTGTTTGGCCTATTTGACAGGATTTGTCTGCTTTCTAATGGAAATACCCTTTTCTTTGGAGAAACATTGTCTTGCTTGCAGCACTTCTCAAATGCTGGATTTCCTTGTCCGATTATGCAAAGTCCTTCCGATCACTTTCTGCGTGCAATAAATACAGATTTTGATAGGATCATTGCAATGTGCAAGAACTGGCAGGATGACAATGGGGACTTCTCATCCGTAAACATGGACACGGCTGTGGCAATACGCACCCTTGAAGCAACTTATAGATCATCAGCTGATGCTGCTGCAGTTGAAAATATGATACTAAGACTCACAGAGAAGGAAGGTCCATTACTTAAAAGCAAGGGAAAAGCTGGCAGTGCTACACGGATAGCAGTCTTAACTTGGAGATCGTTATTGATAATGTCAAGGGAATGGAAATACTACTGGCTTCGACTTATTCTTTATATCATTTTTACGCTCTCTGTTGGTACAGCATTTTCTGGCTTAGGGCATTCTTTGTCTTCAGTTGTGGTGAGTATCTTAATATCACTTTTAGAACAGGATATCACTGGCATCTTTTGCCAGATTTTCCCCCTCTTTAATTTTCGTTTAATGAAAACTTTTTGTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031224 GO:0042623 GO:0042626 GO:0043492 GO:0044425 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

516

Amino Acids

56.83

Weight (kDa)

8.97

Isoelectric Point (pI)

48.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	134 - 275	4.7e-18	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008741)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28070 AT2G28070
fragaria_vesca	FvH4_1g24480 FvH4_1g24480 FvH4_1g24480 FvH4_1g24480 FvH4_1g24480 FvH4_1g24480
malus_domestica	MD00G1165400.v1.1 MD03G1125800.v1.1
prunus_persica	Prupe.6G107900_v2.0.a1 Prupe.6G107900_v2.0.a1
pyrus_communis	pycom11g11830
rosa_chinensis	RchiOBHm_Chr5g0057821
rosa_laevigata	RLG00000035180
rosa_multiflora	Rmu_sc0010457.1_g000004
rosa_roxburghii	Rroxscaffold_1G00022680
rosa_rugosa	Rorug05G0309800
rosa_samantha	Rh5AG377900 Rh5BG389200 Rh5CG413500 Rh5DG404100
rosa_wichuraiana	Rw5G035660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	101
AccB1I	GGYRCC	1 cut(s)	73
AccII	CGCG	1 cut(s)	765
AclWI	GGATC	2 cut(s)	592, 1106
AcoI	YGGCCR	1 cut(s)	721
AcuI	CTGAAG	2 cut(s)	611, 1422
AdeI	CACNNNGTG	1 cut(s)	695
AfaI	GTAC	3 cut(s)	362, 886, 1405
AgsI	TTSAA	7 cut(s)	137, 237, 315, 387, 871, 1187, 1226
AjnI	CCWGG	3 cut(s)	106, 403, 634
AleI	CACNNNNGTG	2 cut(s)	929, 1166
AluBI	AGCT	6 cut(s)	241, 631, 784, 877, 1208, 1286
AluI	AGCT	6 cut(s)	241, 631, 784, 877, 1208, 1286
Alw21I	GWGCWC	1 cut(s)	466
Alw26I	GTCTC	2 cut(s)	226, 847
AlwI	GGATC	2 cut(s)	592, 1106
Ama87I	CYCGRG	1 cut(s)	777
AoxI	GGCC	4 cut(s)	721, 739, 794, 937
ApeKI	GCWGC	4 cut(s)	921, 1009, 1214, 1217
AspLEI	GCGC	2 cut(s)	102, 765
AspS9I	GGNCC	3 cut(s)	252, 428, 1259
AsuHPI	GGTGA	6 cut(s)	135, 203, 515, 707, 821, 1459
AvaI	CYCGRG	1 cut(s)	777
AvaII	GGWCC	3 cut(s)	252, 428, 1259
BaeGI	GKGCMC	1 cut(s)	78
BaeI	ACNNNNGTAYC	2 cut(s)	150, 183
BalI	TGGCCA	1 cut(s)	723
BanI	GGYRCC	1 cut(s)	73
BarI	GAAGNNNNNNTAC	2 cut(s)	1248, 1280
BbsI	GAAGAC	4 cut(s)	30, 144, 481, 1428
Bbv12I	GWGCWC	1 cut(s)	466
BbvI	GCAGC	4 cut(s)	933, 1021, 1201, 1204
BccI	CCATC	3 cut(s)	235, 268, 853
BceAI	ACGGC	1 cut(s)	1179
BciT130I	CCWGG	3 cut(s)	108, 405, 636
BciVI	GTATCC	1 cut(s)	151
BcoDI	GTCTC	2 cut(s)	226, 847
BfmI	CTRYAG	2 cut(s)	288, 1218
BfuI	GTATCC	1 cut(s)	151
BisI	GCNGC	4 cut(s)	922, 1010, 1215, 1218
BlsI	GCNGC	4 cut(s)	923, 1011, 1216, 1219
BmcAI	AGTACT	1 cut(s)	362
Bme1390I	CCNGG	3 cut(s)	108, 405, 636
Bme18I	GGWCC	3 cut(s)	252, 428, 1259
BmeT110I	CYCGRG	1 cut(s)	777
BmgT120I	GGNCC	3 cut(s)	252, 428, 1259
BmiI	GGNNCC	3 cut(s)	75, 253, 429
BmrFI	CCNGG	3 cut(s)	108, 405, 636
BmrI	ACTGGG	1 cut(s)	701
BmsI	GCATC	4 cut(s)	525, 661, 1201, 1497
BmuI	ACTGGG	1 cut(s)	701
BpiI	GAAGAC	4 cut(s)	30, 144, 481, 1428
Bpu10I	CCTNAGC	1 cut(s)	1420
BpuEI	CTTGAG	1 cut(s)	64
Bsa29I	ATCGAT	1 cut(s)	533
BsaI	GGTCTC	1 cut(s)	226
BsaWI	WCCGGW	1 cut(s)	217
BsaXI	ACNNNNNCTCC	2 cut(s)	593, 623
Bse1I	ACTGG	4 cut(s)	696, 1124, 1364, 1489
Bse3DI	GCAATG	5 cut(s)	204, 398, 771, 1101, 1113
BseBI	CCWGG	3 cut(s)	108, 405, 636
BseCI	ATCGAT	1 cut(s)	533
BseGI	GGATG	4 cut(s)	260, 676, 1132, 1145
BseMI	GCAATG	5 cut(s)	204, 398, 771, 1101, 1113
BseMII	CTCAG	1 cut(s)	357
BseNI	ACTGG	4 cut(s)	696, 1124, 1364, 1489
BseSI	GKGCMC	1 cut(s)	78
BseXI	GCAGC	4 cut(s)	933, 1021, 1201, 1204
BsgI	GTGCAG	1 cut(s)	831
Bsh1236I	CGCG	1 cut(s)	765
BshFI	GGCC	4 cut(s)	723, 741, 796, 939
BshNI	GGYRCC	1 cut(s)	73
BshVI	ATCGAT	1 cut(s)	533
BsiHKAI	GWGCWC	1 cut(s)	466
BsiHKCI	CYCGRG	1 cut(s)	777
BsiSI	CCGG	1 cut(s)	218
BslFI	GGGAC	1 cut(s)	1151
BsmAI	GTCTC	2 cut(s)	226, 847
BsmFI	GGGAC	1 cut(s)	1151
BsmI	GAATGC	1 cut(s)	1426
BsnI	GGCC	4 cut(s)	723, 741, 796, 939
Bso31I	GGTCTC	1 cut(s)	226
BsoBI	CYCGRG	1 cut(s)	777
Bsp1286I	GDGCHC	2 cut(s)	78, 466
Bsp143I	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
BspANI	GGCC	4 cut(s)	723, 741, 796, 939
BspCNI	CTCAG	1 cut(s)	358
BspDI	ATCGAT	1 cut(s)	533
BspFNI	CGCG	1 cut(s)	765
BspLI	GGNNCC	3 cut(s)	75, 253, 429
BspMAI	CTGCAG	1 cut(s)	1222
BspPI	GGATC	2 cut(s)	592, 1106
BspT107I	GGYRCC	1 cut(s)	73
BspTNI	GGTCTC	1 cut(s)	226
BsrDI	GCAATG	5 cut(s)	204, 398, 771, 1101, 1113
BsrI	ACTGG	4 cut(s)	696, 1124, 1364, 1489
BssMI	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
Bst2UI	CCWGG	3 cut(s)	108, 405, 636
Bst4CI	ACNGT	8 cut(s)	15, 21, 289, 337, 418, 521, 595, 728
Bst6I	CTCTTC	1 cut(s)	374
BstAPI	GCANNNNNTGC	1 cut(s)	1494
BstC8I	GCNNGC	5 cut(s)	55, 879, 1007, 1074, 1288
BstDEI	CTNAG	4 cut(s)	366, 458, 1238, 1420
BstF5I	GGATG	4 cut(s)	260, 676, 1132, 1145
BstFNI	CGCG	1 cut(s)	765
BstHHI	GCGC	2 cut(s)	102, 765
BstKTI	GATC	5 cut(s)	587, 1063, 1101, 1202, 1325
BstMAI	GTCTC	2 cut(s)	226, 847
BstMBI	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
BstMWI	GCNNNNNNNGC	5 cut(s)	238, 680, 1214, 1292, 1494
BstNI	CCWGG	3 cut(s)	108, 405, 636
BstSCI	CCNGG	3 cut(s)	106, 403, 634
BstSFI	CTRYAG	2 cut(s)	288, 1218
BstSLI	GKGCMC	1 cut(s)	78
BstUI	CGCG	1 cut(s)	765
BstV1I	GCAGC	4 cut(s)	933, 1021, 1201, 1204
BstV2I	GAAGAC	4 cut(s)	30, 144, 481, 1428
BstX2I	RGATCY	1 cut(s)	584
BstXI	CCANNNNNNTGG	1 cut(s)	691
BstYI	RGATCY	1 cut(s)	584
Bsu15I	ATCGAT	1 cut(s)	533
BsuI	GTATCC	1 cut(s)	151
BsuRI	GGCC	4 cut(s)	723, 741, 796, 939
BsuTUI	ATCGAT	1 cut(s)	533
BtgZI	GCGATG	1 cut(s)	213
BtsCI	GGATG	4 cut(s)	260, 676, 1132, 1145
BtsI	GCAGTG	1 cut(s)	1297
BtsIMutI	CAGTG	5 cut(s)	591, 689, 724, 1297, 1482
Cac8I	GCNNGC	5 cut(s)	55, 879, 1007, 1074, 1288
CfoI	GCGC	2 cut(s)	102, 765
Cfr13I	GGNCC	3 cut(s)	252, 428, 1259
ClaI	ATCGAT	1 cut(s)	533
CsiI	ACCWGGT	1 cut(s)	106
Csp6I	GTAC	3 cut(s)	361, 885, 1404
CviAII	CATG	4 cut(s)	680, 685, 800, 1156
CviQI	GTAC	3 cut(s)	361, 885, 1404
DdeI	CTNAG	4 cut(s)	366, 458, 1238, 1420
DpnI	GATC	5 cut(s)	586, 1062, 1100, 1201, 1324
DpnII	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
DraIII	CACNNNGTG	1 cut(s)	695
EaeI	YGGCCR	1 cut(s)	721
Eam1104I	CTCTTC	1 cut(s)	374
EarI	CTCTTC	1 cut(s)	374
Eco147I	AGGCCT	2 cut(s)	741, 796
Eco31I	GGTCTC	1 cut(s)	226
Eco32I	GATATC	1 cut(s)	1480
Eco47I	GGWCC	3 cut(s)	252, 428, 1259
Eco57I	CTGAAG	2 cut(s)	611, 1422
Eco88I	CYCGRG	1 cut(s)	777
EcoO109I	RGGNCCY	2 cut(s)	252, 428
EcoRII	CCWGG	3 cut(s)	106, 403, 634
EcoRV	GATATC	1 cut(s)	1480
EcoT22I	ATGCAT	1 cut(s)	400
FaeI	CATG	4 cut(s)	683, 688, 803, 1159
FaqI	GGGAC	1 cut(s)	1151
FatI	CATG	4 cut(s)	679, 684, 799, 1155
FauNDI	CATATG	2 cut(s)	541, 550
Fnu4HI	GCNGC	4 cut(s)	922, 1010, 1215, 1218
FokI	GGATG	4 cut(s)	247, 683, 1132, 1139
Fsp4HI	GCNGC	4 cut(s)	922, 1010, 1215, 1218
FspI	TGCGCA	1 cut(s)	101
GlaI	GCGC	2 cut(s)	101, 764
GluI	GCNGC	4 cut(s)	922, 1010, 1215, 1218
HaeIII	GGCC	4 cut(s)	723, 741, 796, 939
HapII	CCGG	1 cut(s)	218
HhaI	GCGC	2 cut(s)	102, 765
Hin1II	CATG	4 cut(s)	683, 688, 803, 1159
Hin6I	GCGC	2 cut(s)	100, 763
HinP1I	GCGC	2 cut(s)	100, 763
HindIII	AAGCTT	1 cut(s)	875
HinfI	GANTC	3 cut(s)	140, 194, 1242
HpaII	CCGG	1 cut(s)	218
HphI	GGTGA	6 cut(s)	135, 203, 515, 707, 821, 1459
Hpy166II	GTNNAC	3 cut(s)	450, 517, 1153
Hpy188I	TCNGA	7 cut(s)	25, 119, 367, 651, 855, 1041, 1060
Hpy188III	TCNNGA	1 cut(s)	599
Hpy8I	GTNNAC	3 cut(s)	450, 517, 1153
HpyAV	CCTTC	8 cut(s)	179, 319, 635, 752, 753, 1065, 1246, 1250
HpyCH4III	ACNGT	8 cut(s)	15, 21, 289, 337, 418, 521, 595, 728
HpyCH4IV	ACGT	1 cut(s)	497
HpyF10VI	GCNNNNNNNGC	5 cut(s)	238, 680, 1214, 1292, 1494
HpyF3I	CTNAG	4 cut(s)	366, 458, 1238, 1420
HpySE526I	ACGT	1 cut(s)	497
Hsp92II	CATG	4 cut(s)	683, 688, 803, 1159
HspAI	GCGC	2 cut(s)	100, 763
Kzo9I	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
Lsp1109I	GCAGC	4 cut(s)	933, 1021, 1201, 1204
LweI	GCATC	4 cut(s)	525, 661, 1201, 1497
MabI	ACCWGGT	1 cut(s)	106
MaeII	ACGT	1 cut(s)	497
MaeIII	GTNAC	5 cut(s)	15, 191, 337, 687, 862
MalI	GATC	5 cut(s)	586, 1062, 1100, 1201, 1324
MboI	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
MboII	GAAGA	9 cut(s)	17, 30, 33, 149, 194, 391, 486, 883, 1428
MfeI	CAATTG	1 cut(s)	315
MflI	RGATCY	1 cut(s)	584
MhlI	GDGCHC	2 cut(s)	78, 466
MlsI	TGGCCA	1 cut(s)	723
MluCI	AATT	8 cut(s)	28, 85, 315, 420, 574, 579, 1516, 1543
MluNI	TGGCCA	1 cut(s)	723
MlyI	GAGTC	3 cut(s)	134, 188, 1236
Mox20I	TGGCCA	1 cut(s)	723
Mph1103I	ATGCAT	1 cut(s)	400
MscI	TGGCCA	1 cut(s)	723
MseI	TTAA	7 cut(s)	561, 578, 1269, 1313, 1457, 1515, 1526
MslI	CAYNNNNRTG	4 cut(s)	148, 929, 1106, 1166
Msp20I	TGGCCA	1 cut(s)	723
MspA1I	CMGCKG	1 cut(s)	1208
MspI	CCGG	1 cut(s)	218
MspR9I	CCNGG	3 cut(s)	108, 405, 636
MunI	CAATTG	1 cut(s)	315
Mva1269I	GAATGC	1 cut(s)	1426
MvaI	CCWGG	3 cut(s)	108, 405, 636
MvnI	CGCG	1 cut(s)	765
MwoI	GCNNNNNNNGC	5 cut(s)	238, 680, 1214, 1292, 1494
NdeI	CATATG	2 cut(s)	541, 550
NdeII	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
NlaIII	CATG	4 cut(s)	683, 688, 803, 1159
NlaIV	GGNNCC	3 cut(s)	75, 253, 429
NmuCI	GTSAC	3 cut(s)	15, 191, 687
NsbI	TGCGCA	1 cut(s)	101
NsiI	ATGCAT	1 cut(s)	400
OliI	CACNNNNGTG	2 cut(s)	929, 1166
PceI	AGGCCT	2 cut(s)	741, 796
PctI	GAATGC	1 cut(s)	1426
PkrI	GCNGC	4 cut(s)	923, 1011, 1216, 1219
PleI	GAGTC	3 cut(s)	134, 188, 1236
PpsI	GAGTC	3 cut(s)	134, 188, 1236
PpuMI	RGGWCCY	2 cut(s)	252, 428
Psp5II	RGGWCCY	2 cut(s)	252, 428
Psp6I	CCWGG	3 cut(s)	106, 403, 634
PspGI	CCWGG	3 cut(s)	106, 403, 634
PspN4I	GGNNCC	3 cut(s)	75, 253, 429
PspPI	GGNCC	3 cut(s)	252, 428, 1259
PspPPI	RGGWCCY	2 cut(s)	252, 428
PstI	CTGCAG	1 cut(s)	1222
PsuI	RGATCY	1 cut(s)	584
PvuII	CAGCTG	1 cut(s)	1208
RsaI	GTAC	3 cut(s)	362, 886, 1405
RsaNI	GTAC	3 cut(s)	361, 885, 1404
RseI	CAYNNNNRTG	4 cut(s)	148, 929, 1106, 1166
SaqAI	TTAA	7 cut(s)	561, 578, 1269, 1313, 1457, 1515, 1526
SatI	GCNGC	4 cut(s)	922, 1010, 1215, 1218
Sau3AI	GATC	5 cut(s)	584, 1060, 1098, 1199, 1322
Sau96I	GGNCC	3 cut(s)	252, 428, 1259
ScaI	AGTACT	1 cut(s)	362
SchI	GAGTC	3 cut(s)	134, 188, 1236
ScrFI	CCNGG	3 cut(s)	108, 405, 636
SduI	GDGCHC	2 cut(s)	78, 466
SexAI	ACCWGGT	1 cut(s)	106
SfaNI	GCATC	4 cut(s)	525, 661, 1201, 1497
SfcI	CTRYAG	2 cut(s)	288, 1218
SinI	GGWCC	3 cut(s)	252, 428, 1259
SmiMI	CAYNNNNRTG	4 cut(s)	148, 929, 1106, 1166
SmlI	CTYRAG	1 cut(s)	79
SmoI	CTYRAG	1 cut(s)	79
Sse9I	AATT	8 cut(s)	28, 85, 315, 420, 574, 579, 1516, 1543
SseBI	AGGCCT	2 cut(s)	741, 796
StuI	AGGCCT	2 cut(s)	741, 796
StyD4I	CCNGG	3 cut(s)	106, 403, 634
TaaI	ACNGT	8 cut(s)	15, 21, 289, 337, 418, 521, 595, 728
TaiI	ACGT	1 cut(s)	500
TaqI	TCGA	2 cut(s)	533, 1366
TasI	AATT	8 cut(s)	28, 85, 315, 420, 574, 579, 1516, 1543
TatI	WGTACW	2 cut(s)	360, 884
Tru1I	TTAA	7 cut(s)	561, 578, 1269, 1313, 1457, 1515, 1526
Tru9I	TTAA	7 cut(s)	561, 578, 1269, 1313, 1457, 1515, 1526
TscAI	CASTG	5 cut(s)	598, 696, 731, 1297, 1489
TseFI	GTSAC	3 cut(s)	15, 191, 687
TseI	GCWGC	4 cut(s)	921, 1009, 1214, 1217
Tsp45I	GTSAC	3 cut(s)	15, 191, 687
TspDTI	ATGAA	6 cut(s)	33, 537, 668, 749, 799, 1544
TspGWI	ACGGA	2 cut(s)	1138, 1315
TspRI	CASTG	5 cut(s)	598, 696, 731, 1297, 1489
VpaK11BI	GGWCC	3 cut(s)	252, 428, 1259
ZrmI	AGTACT	1 cut(s)	362
Zsp2I	ATGCAT	1 cut(s)	400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.