RLG00000035849

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016710
Physical Location & Seq
Forward (+)
72588375 .. 72590528
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035849

Sequence Viewer

Length: 1551 bp
ATGGCTCAACTTGAACGTCCGCAAAGACATGGTGATTCTGAAACTACAATGACCTCTGATCATAAAAAACTTGCTGTCAACCCTACTATGGAACACTATCTTCGAGGAACTGAATTTTCAAGGTCGTCACCAACATCTTTATCATCTCCAGTTGGAAGCCATGATGCAGAAGAAGATCATAACCACCATCCCAAGAAATCAGTCCTCACCAAGGTGAAAGAGAAAGCTAAGAAACTGAAGCACTCACTCAGCTACAAGAAGAAACATCATGACAATGATAGCCATGAGACCGATAACATCACCCCTTCATGGAGTGCTAACTTGAATGATAATGAAGAAGAAGAAGAAGAAGATGCTGAATACCTAGGAGCCCCAATGTACAAATCAGAGATGGCACCTGAAGGATACAGGGACACCGCAAAGTTAGCAACTCCCGTGGTTTCTGAGAAGCGTGTTTTGTCGAGCAGTGTTGGTCAGAGCTCTGAACAAAATAAGGAGCTGCAGTCTCCTAGTTCTAACCAGATAGTAGCCGAAAGTGCGATCATGAAGCTTCCCAATGAGATATTTACAGGAATTGTTCTCACGAGAACTCTTAGTACTAACAAGACAGCAACTGGCACCACAGCCACAAAGCCTCATAGTCCTAACAAGACAGCGAGTGAAACTTCGCCTAACAAGTCTCACAGTACTTCGCCTAACAAGCCTCACAGTCCCAGCAAGACAGTAACCGAAACTGGCGCCACAAAGACTCCTAGTCCTAAGAAGACAATAGCTGAGACTGTGACCGAGAAGTTGGGAGCAGGCTATGCCACCGTATCGGAGAAGTTGGGAACAGGCTACGCCACCGTATCGGAGAAGTTGGGAACAGGCTACAACACAGCATCAGAAAAGTTGGGAACAGGCTACAATGCAGCATCAGAGAAGTTAGGACCAGCTTATGCCACAGTTACAGATGCAACACATGCAATTGCCTCCAAGATAGAAAGTCTCACTGTTTCACCACCAGTTACCACAACGAGAGAAAATCTCATTTCTTCACACCCAGATCTCACGAAAATACATGGTCTCGCTGTTACAGCCCCACCACCTTCACAGAGCATTACAGCTCAGGAAGCTCCACAAGCCTTGTCAGCATCAGCAGTCGAGCATAAGTCTTGGACTCGTGGAGCTCTAGAGGATCCACAACCTTCTTCAGCCCCAGCAGCTCCTGGAAAGACTACTGCCGAACAAAGATGGGATAAGGGTGTATCTGTAAAGGAGTATTTGATTCAAAAGTTCGAGCCCGGGGAAGATGAGAGAGCACTTTCACAAGTGATATCTGATGCAATGAGTCCGAGGAAAAGTCCTGGTGAAGCGGGTGTGGTGGAGAAGGTGAAAGGGGCTATAACTTCATTGCTTCGGAACGACGAATCCCCCAGAACTACTACTTCACAGTCAACCTTGTCCCCATCACAGTCAGCCTTGTCCTCATCACCACGCATTCCAATCTCTACCAATGCTTATGAAGTCGTTGAGGAAGAAAATCAGGGAAGAATACTTCAGGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

517

Amino Acids

55.33

Weight (kDa)

6.34

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTI65_LTI78_N PF23403 61 - 130 8.5e-13 LTI65/LTI78 CAP160 N-terminal
LTI65_PGEED PF23399 413 - 443 3e-18 LTI65/LTI78 PGEED repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016710)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46750
fragaria_vesca FvH4_3g36910
malus_domestica MD11G1095400.v1.1
prunus_persica Prupe.6G070900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0066301
rosa_laevigata RLG00000035849
rosa_multiflora Rmu_sc0006887.1_g000013
rosa_roxburghii Rroxscaffold_1G00014820
rosa_rugosa Rorug05G0377000
rosa_samantha Rh5AG435600 Rh5BG451800 Rh5CG474100 Rh5DG466700
rosa_wichuraiana Rw5G040790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 394, 617, 737
AciI CCGC 3 cut(s) 20, 417, 1355
AclWI GGATC 2 cut(s) 1172, 1185
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 4 cut(s) 257, 420, 1176, 1523
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 3 cut(s) 380, 598, 688
AfiI CCNNNNNNNGG 3 cut(s) 88, 211, 309
AgsI TTSAA 4 cut(s) 14, 120, 325, 1271
AhdI GACNNNNNGTC 1 cut(s) 753
AjnI CCWGG 2 cut(s) 1207, 1345
AleI CACNNNNGTG 1 cut(s) 212
AloI GAACNNNNNNTCC 2 cut(s) 1394, 1426
Alw21I GWGCWC 3 cut(s) 482, 1171, 1303
Alw26I GTCTC 6 cut(s) 281, 510, 684, 770, 992, 1070
AlwI GGATC 2 cut(s) 1172, 1185
AlwNI CAGNNNCTG 2 cut(s) 614, 1208
Ama87I CYCGRG 1 cut(s) 1283
ApeKI GCWGC 3 cut(s) 499, 911, 1202
ApoI RAATTY 1 cut(s) 113
AspA2I CCTAGG 1 cut(s) 364
AspLEI GCGC 1 cut(s) 740
AspS9I GGNCC 1 cut(s) 929
AsuC2I CCSGG 2 cut(s) 1284, 1285
AsuHPI GGTGA 9 cut(s) 44, 120, 199, 226, 292, 990, 1361, 1384, 1464
AvaI CYCGRG 1 cut(s) 1283
AvaII GGWCC 1 cut(s) 929
AvrII CCTAGG 1 cut(s) 364
BamHI GGATCC 1 cut(s) 1177
BanI GGYRCC 3 cut(s) 394, 617, 737
BanII GRGCYC 4 cut(s) 373, 482, 1171, 1284
BauI CACGAG 2 cut(s) 583, 1161
BbsI GAAGAC 1 cut(s) 770
Bbv12I GWGCWC 3 cut(s) 482, 1171, 1303
BbvI GCAGC 3 cut(s) 486, 923, 1214
BccI CCATC 4 cut(s) 195, 385, 1227, 1456
BciT130I CCWGG 2 cut(s) 1209, 1347
BciVI GTATCC 1 cut(s) 398
BclI TGATCA 1 cut(s) 58
BcnI CCSGG 2 cut(s) 1284, 1285
BcoDI GTCTC 6 cut(s) 281, 510, 684, 770, 992, 1070
BfaI CTAG 4 cut(s) 365, 510, 753, 1172
BfmI CTRYAG 1 cut(s) 500
BfoI RGCGCY 1 cut(s) 741
BfuI GTATCC 1 cut(s) 398
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 3 cut(s) 500, 912, 1203
BlnI CCTAGG 1 cut(s) 364
BlsI GCNGC 3 cut(s) 501, 913, 1204
BmcAI AGTACT 2 cut(s) 598, 688
Bme1390I CCNGG 4 cut(s) 1209, 1284, 1285, 1347
Bme18I GGWCC 1 cut(s) 929
BmeRI GACNNNNNGTC 1 cut(s) 753
BmeT110I CYCGRG 1 cut(s) 1283
BmgT120I GGNCC 1 cut(s) 929
BmiI GGNNCC 5 cut(s) 370, 396, 619, 739, 1179
BmrFI CCNGG 4 cut(s) 1209, 1284, 1285, 1347
BmsI GCATC 7 cut(s) 154, 343, 890, 923, 943, 1142, 1312
BpiI GAAGAC 1 cut(s) 770
BplI GAGNNNNNCTC 2 cut(s) 1011, 1043
BpmI CTGGAG 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 1107
BpuMI CCSGG 2 cut(s) 1284, 1285
BsaHI GRCGYC 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 281, 1070
BsaJI CCNNGG 6 cut(s) 210, 364, 435, 1283, 1284, 1334
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 733, 763
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 211, 309
Bse1I ACTGG 4 cut(s) 149, 619, 739, 1004
Bse3DI GCAATG 2 cut(s) 1332, 1391
BseBI CCWGG 2 cut(s) 1209, 1347
BseDI CCNNGG 6 cut(s) 210, 364, 435, 1283, 1284, 1334
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 3 cut(s) 88, 211, 309
BseMI GCAATG 2 cut(s) 1332, 1391
BseMII CTCAG 4 cut(s) 262, 435, 765, 1121
BseNI ACTGG 4 cut(s) 149, 619, 739, 1004
BseXI GCAGC 3 cut(s) 486, 923, 1214
BseYI CCCAGC 2 cut(s) 713, 1198
BshNI GGYRCC 3 cut(s) 394, 617, 737
BsiHKAI GWGCWC 3 cut(s) 482, 1171, 1303
BsiHKCI CYCGRG 1 cut(s) 1283
BsiSI CCGG 1 cut(s) 1284
BslFI GGGAC 3 cut(s) 425, 696, 1429
BslI CCNNNNNNNGG 3 cut(s) 88, 211, 309
BsmAI GTCTC 6 cut(s) 281, 510, 684, 770, 992, 1070
BsmFI GGGAC 3 cut(s) 425, 696, 1429
BsmI GAATGC 1 cut(s) 1479
Bso31I GGTCTC 2 cut(s) 281, 1070
BsoBI CYCGRG 1 cut(s) 1283
Bsp1286I GDGCHC 5 cut(s) 373, 482, 1171, 1284, 1303
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 5 cut(s) 58, 175, 540, 1045, 1177
BspACI CCGC 3 cut(s) 20, 417, 1355
BspCNI CTCAG 4 cut(s) 261, 436, 766, 1120
BspHI TCATGA 2 cut(s) 268, 543
BspLI GGNNCC 5 cut(s) 370, 396, 619, 739, 1179
BspMAI CTGCAG 1 cut(s) 504
BspPI GGATC 2 cut(s) 1172, 1185
BspT107I GGYRCC 3 cut(s) 394, 617, 737
BspTNI GGTCTC 2 cut(s) 281, 1070
BsrDI GCAATG 2 cut(s) 1332, 1391
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 4 cut(s) 149, 619, 739, 1004
BssECI CCNNGG 6 cut(s) 210, 364, 435, 1283, 1284, 1334
BssMI GATC 5 cut(s) 58, 175, 540, 1045, 1177
BssNI GRCGYC 1 cut(s) 738
BssSI CACGAG 2 cut(s) 583, 1161
BssT1I CCWWGG 2 cut(s) 210, 364
Bst2BI CACGAG 2 cut(s) 583, 1161
Bst2UI CCWGG 2 cut(s) 1209, 1347
BstACI GRCGYC 1 cut(s) 738
BstAPI GCANNNNNTGC 2 cut(s) 806, 962
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 7 cut(s) 228, 248, 444, 593, 759, 774, 1107
BstDSI CCRYGG 1 cut(s) 435
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 1 cut(s) 740
BstKTI GATC 5 cut(s) 61, 178, 543, 1048, 1180
BstMAI GTCTC 6 cut(s) 281, 510, 684, 770, 992, 1070
BstMBI GATC 5 cut(s) 58, 175, 540, 1045, 1177
BstNI CCWGG 2 cut(s) 1209, 1347
BstNSI RCATGY 1 cut(s) 965
BstSCI CCNGG 4 cut(s) 1207, 1282, 1283, 1345
BstSFI CTRYAG 1 cut(s) 500
BstV1I GCAGC 3 cut(s) 486, 923, 1214
BstV2I GAAGAC 1 cut(s) 770
BstX2I RGATCY 2 cut(s) 1045, 1177
BstYI RGATCY 2 cut(s) 1045, 1177
BsuI GTATCC 1 cut(s) 398
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 1 cut(s) 187
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 472, 990
Cac8I GCNNGC 1 cut(s) 802
CaiI CAGNNNCTG 2 cut(s) 614, 1208
CciI TCATGA 2 cut(s) 268, 543
CfoI GCGC 1 cut(s) 740
Cfr13I GGNCC 1 cut(s) 929
Cfr9I CCCGGG 1 cut(s) 1283
Csp6I GTAC 3 cut(s) 379, 597, 687
CspCI CAANNNNNGTGG 4 cut(s) 417, 452, 1107, 1142
CviAII CATG 8 cut(s) 29, 161, 269, 284, 309, 544, 962, 1061
CviQI GTAC 3 cut(s) 379, 597, 687
DdeI CTNAG 7 cut(s) 228, 248, 444, 593, 759, 774, 1107
DinI GGCGCC 1 cut(s) 739
DpnI GATC 5 cut(s) 60, 177, 542, 1047, 1179
DpnII GATC 5 cut(s) 58, 175, 540, 1045, 1177
DriI GACNNNNNGTC 1 cut(s) 753
Eam1105I GACNNNNNGTC 1 cut(s) 753
Ecl136II GAGCTC 2 cut(s) 480, 1169
Eco130I CCWWGG 2 cut(s) 210, 364
Eco24I GRGCYC 4 cut(s) 373, 482, 1171, 1284
Eco31I GGTCTC 2 cut(s) 281, 1070
Eco32I GATATC 1 cut(s) 1317
Eco47I GGWCC 1 cut(s) 929
Eco53kI GAGCTC 2 cut(s) 480, 1169
Eco57I CTGAAG 4 cut(s) 257, 420, 1176, 1523
Eco88I CYCGRG 1 cut(s) 1283
EcoICRI GAGCTC 2 cut(s) 480, 1169
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 1207, 1345
EcoRV GATATC 1 cut(s) 1317
EcoT14I CCWWGG 2 cut(s) 210, 364
EcoT38I GRGCYC 4 cut(s) 373, 482, 1171, 1284
EgeI GGCGCC 1 cut(s) 739
EheI GGCGCC 1 cut(s) 739
ErhI CCWWGG 2 cut(s) 210, 364
FaeI CATG 8 cut(s) 32, 164, 272, 287, 312, 547, 965, 1064
FaqI GGGAC 3 cut(s) 425, 696, 1429
FatI CATG 8 cut(s) 28, 160, 268, 283, 308, 543, 961, 1060
FauI CCCGC 1 cut(s) 1348
FbaI TGATCA 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 500, 912, 1203
FokI GGATG 1 cut(s) 174
FriOI GRGCYC 4 cut(s) 373, 482, 1171, 1284
Fsp4HI GCNGC 3 cut(s) 500, 912, 1203
FspBI CTAG 4 cut(s) 365, 510, 753, 1172
GlaI GCGC 1 cut(s) 739
GluI GCNGC 3 cut(s) 500, 912, 1203
GsaI CCCAGC 2 cut(s) 717, 1202
GsuI CTGGAG 1 cut(s) 132
HaeII RGCGCY 1 cut(s) 741
HapII CCGG 1 cut(s) 1284
HhaI GCGC 1 cut(s) 740
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 8 cut(s) 32, 164, 272, 287, 312, 547, 965, 1064
Hin6I GCGC 1 cut(s) 738
HinP1I GCGC 1 cut(s) 738
HincII GTYRAC 2 cut(s) 79, 1437
HindII GTYRAC 2 cut(s) 79, 1437
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 6 cut(s) 35, 748, 1159, 1267, 1330, 1409
HpaII CCGG 1 cut(s) 1284
HphI GGTGA 9 cut(s) 44, 120, 199, 226, 292, 990, 1361, 1384, 1464
Hpy166II GTNNAC 2 cut(s) 79, 1437
Hpy188III TCNNGA 6 cut(s) 269, 544, 583, 1051, 1109, 1172
Hpy8I GTNNAC 2 cut(s) 79, 1437
Hpy99I CGWCG 1 cut(s) 1409
HpyAV CCTTC 5 cut(s) 315, 395, 1098, 1197, 1363
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 6 cut(s) 167, 502, 911, 956, 965, 1325
HpyF3I CTNAG 7 cut(s) 228, 248, 444, 593, 759, 774, 1107
HpySE526I ACGT 1 cut(s) 16
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 8 cut(s) 32, 164, 272, 287, 312, 547, 965, 1064
HspAI GCGC 1 cut(s) 738
KasI GGCGCC 1 cut(s) 737
Ksp22I TGATCA 1 cut(s) 58
Kzo9I GATC 5 cut(s) 58, 175, 540, 1045, 1177
LmnI GCTCC 6 cut(s) 368, 496, 797, 1120, 1166, 1210
Lsp1109I GCAGC 3 cut(s) 486, 923, 1214
LweI GCATC 7 cut(s) 154, 343, 890, 923, 943, 1142, 1312
MaeI CTAG 4 cut(s) 365, 510, 753, 1172
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 6 cut(s) 126, 724, 781, 946, 1006, 1072
MalI GATC 5 cut(s) 60, 177, 542, 1047, 1179
MboI GATC 5 cut(s) 58, 175, 540, 1045, 1177
MfeI CAATTG 1 cut(s) 966
MflI RGATCY 2 cut(s) 1045, 1177
MhlI GDGCHC 5 cut(s) 373, 482, 1171, 1284, 1303
MluCI AATT 4 cut(s) 113, 573, 966, 1546
Mly113I GGCGCC 1 cut(s) 738
MlyI GAGTC 3 cut(s) 742, 1153, 1339
MmeI TCCRAC 1 cut(s) 133
MslI CAYNNNNRTG 2 cut(s) 212, 273
MspI CCGG 1 cut(s) 1284
MspR9I CCNGG 4 cut(s) 1209, 1284, 1285, 1347
MunI CAATTG 1 cut(s) 966
Mva1269I GAATGC 1 cut(s) 1479
MvaI CCWGG 2 cut(s) 1209, 1347
NarI GGCGCC 1 cut(s) 738
NciI CCSGG 2 cut(s) 1284, 1285
NdeII GATC 5 cut(s) 58, 175, 540, 1045, 1177
NlaIII CATG 8 cut(s) 32, 164, 272, 287, 312, 547, 965, 1064
NlaIV GGNNCC 5 cut(s) 370, 396, 619, 739, 1179
NmuCI GTSAC 2 cut(s) 126, 781
NspI RCATGY 1 cut(s) 965
OliI CACNNNNGTG 1 cut(s) 212
PagI TCATGA 2 cut(s) 268, 543
PctI GAATGC 1 cut(s) 1479
PfeI GAWTC 3 cut(s) 35, 1267, 1409
PfoI TCCNGGA 1 cut(s) 1207
PkrI GCNGC 3 cut(s) 501, 913, 1204
PleI GAGTC 3 cut(s) 742, 1153, 1338
PluTI GGCGCC 1 cut(s) 741
PpsI GAGTC 3 cut(s) 742, 1153, 1338
Psp124BI GAGCTC 2 cut(s) 482, 1171
Psp6I CCWGG 2 cut(s) 1207, 1345
PspFI CCCAGC 2 cut(s) 713, 1198
PspGI CCWGG 2 cut(s) 1207, 1345
PspN4I GGNNCC 5 cut(s) 370, 396, 619, 739, 1179
PspPI GGNCC 1 cut(s) 929
PsrI GAACNNNNNNTAC 2 cut(s) 580, 612
PstI CTGCAG 1 cut(s) 504
PstNI CAGNNNCTG 2 cut(s) 614, 1208
PsuI RGATCY 2 cut(s) 1045, 1177
RsaI GTAC 3 cut(s) 380, 598, 688
RsaNI GTAC 3 cut(s) 379, 597, 687
RseI CAYNNNNRTG 2 cut(s) 212, 273
SacI GAGCTC 2 cut(s) 482, 1171
SatI GCNGC 3 cut(s) 500, 912, 1203
Sau3AI GATC 5 cut(s) 58, 175, 540, 1045, 1177
Sau96I GGNCC 1 cut(s) 929
ScaI AGTACT 2 cut(s) 598, 688
SchI GAGTC 3 cut(s) 742, 1153, 1339
ScrFI CCNGG 4 cut(s) 1209, 1284, 1285, 1347
SduI GDGCHC 5 cut(s) 373, 482, 1171, 1284, 1303
SfaNI GCATC 7 cut(s) 154, 343, 890, 923, 943, 1142, 1312
SfcI CTRYAG 1 cut(s) 500
SfoI GGCGCC 1 cut(s) 739
SinI GGWCC 1 cut(s) 929
SmaI CCCGGG 1 cut(s) 1285
SmiMI CAYNNNNRTG 2 cut(s) 212, 273
Sse9I AATT 4 cut(s) 113, 573, 966, 1546
SsiI CCGC 3 cut(s) 20, 417, 1355
SspDI GGCGCC 1 cut(s) 737
SspMI CTAG 4 cut(s) 365, 510, 753, 1172
SstI GAGCTC 2 cut(s) 482, 1171
StyD4I CCNGG 4 cut(s) 1207, 1282, 1283, 1345
StyI CCWWGG 2 cut(s) 210, 364
TaiI ACGT 1 cut(s) 19
TaqI TCGA 4 cut(s) 103, 461, 1143, 1278
TaqII GACCGA 2 cut(s) 305, 800
TasI AATT 4 cut(s) 113, 573, 966, 1546
TatI WGTACW 3 cut(s) 378, 596, 686
TfiI GAWTC 3 cut(s) 35, 1267, 1409
TscAI CASTG 2 cut(s) 472, 997
TseFI GTSAC 2 cut(s) 126, 781
TseI GCWGC 3 cut(s) 499, 911, 1202
Tsp45I GTSAC 2 cut(s) 126, 781
TspDTI ATGAA 5 cut(s) 297, 348, 560, 1380, 1518
TspMI CCCGGG 1 cut(s) 1283
TspRI CASTG 2 cut(s) 472, 997
VpaK11BI GGWCC 1 cut(s) 929
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 1 cut(s) 113
XbaI TCTAGA 1 cut(s) 1171
XceI RCATGY 1 cut(s) 965
XmaI CCCGGG 1 cut(s) 1283
XmaJI CCTAGG 1 cut(s) 364
XspI CTAG 4 cut(s) 365, 510, 753, 1172
ZrmI AGTACT 2 cut(s) 598, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.