Rmu_co8445381.1_g000001

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8445381.1
OG0023093
Physical Location & Seq
Forward (+)
1134 .. 1583
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8445381.1_g000001.1.cds

Sequence Viewer

Length: 450 bp
atggttggtcacttgcttggaccgaaggctaggtttccaggcttcaagggcaccatacttacgatatggcaagtcaaatctgggctttctatccctgatgtcggagatcgtttcttattccaatttgatcttgaagctgatcgtaatcgagctcttcatggaagaccttcgttttataggaacacgatgctggtggttggtgaatatgatggtgtaagttcgttggaggtcatcccgctgaggatgatggagacctggatggcagtcaaaggtttgcccgtggcgctaaggaacaaggtagctctgactttggtaggttcctttattggacaggtgataggcttcgatcagctggcgctctgtcgtaaggaggaagaacaatgtattagactggtttttgacactcgccggagggttcgaacctcgatgctcttcaagttttcttcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

149

Amino Acids

16.91

Weight (kDa)

9.92

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023093)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8445381.1_g000001 Rmu_sc0003071.1_g000006 Rmu_sc0005193.1_g000004 Rmu_sc0009033.1_g000011

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 50
AciI CCGC 1 cut(s) 236
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 3 cut(s) 46, 134, 436
AjnI CCWGG 2 cut(s) 37, 254
AluBI AGCT 4 cut(s) 137, 152, 302, 352
AluI AGCT 4 cut(s) 137, 152, 302, 352
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 245
Asp700I GAANNNNTTC 1 cut(s) 166
AspLEI GCGC 2 cut(s) 286, 358
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 2 cut(s) 212, 346
AsuII TTCGAA 1 cut(s) 418
AvaII GGWCC 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 53
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 239
BccI CCATC 3 cut(s) 203, 241, 253
BciT130I CCWGG 2 cut(s) 39, 256
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 30
BfoI RGCGCY 2 cut(s) 287, 359
Bme1390I CCNGG 2 cut(s) 39, 256
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 2 cut(s) 52, 319
BmrFI CCNGG 2 cut(s) 39, 256
BmsI GCATC 2 cut(s) 177, 417
BpiI GAAGAC 1 cut(s) 169
Bpu10I CCTNAGC 2 cut(s) 239, 287
Bpu14I TTCGAA 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 2 cut(s) 39, 256
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 3 cut(s) 231, 249, 264
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 396
BseSI GKGCMC 1 cut(s) 53
BshNI GGYRCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 245
Bso31I GGTCTC 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 418
Bsp1286I GDGCHC 2 cut(s) 53, 154
Bsp143I GATC 4 cut(s) 106, 127, 139, 346
BspACI CCGC 1 cut(s) 236
BspCNI CTCAG 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 52, 319
BspQI GCTCTTC 2 cut(s) 159, 437
BspT104I TTCGAA 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 396
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 4 cut(s) 106, 127, 139, 346
Bst2UI CCWGG 2 cut(s) 39, 256
Bst6I CTCTTC 2 cut(s) 159, 437
BstBI TTCGAA 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 239, 287
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 231, 249, 264
BstH2I RGCGCY 2 cut(s) 287, 359
BstHHI GCGC 2 cut(s) 286, 358
BstKTI GATC 4 cut(s) 109, 130, 142, 349
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 4 cut(s) 106, 127, 139, 346
BstMWI GCNNNNNNNGC 2 cut(s) 48, 283
BstNI CCWGG 2 cut(s) 39, 256
BstSCI CCNGG 2 cut(s) 37, 254
BstSLI GKGCMC 1 cut(s) 53
BstV2I GAAGAC 1 cut(s) 169
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 3 cut(s) 231, 249, 264
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 2 cut(s) 286, 358
Cfr13I GGNCC 1 cut(s) 20
CviAII CATG 1 cut(s) 158
CviJI RGCY 8 cut(s) 29, 42, 85, 137, 152, 302, 342, 352
CviKI_1 RGCY 8 cut(s) 29, 42, 85, 137, 152, 302, 342, 352
DdeI CTNAG 2 cut(s) 239, 287
DpnI GATC 4 cut(s) 108, 129, 141, 348
DpnII GATC 4 cut(s) 106, 127, 139, 346
Eam1104I CTCTTC 2 cut(s) 159, 437
EarI CTCTTC 2 cut(s) 159, 437
Ecl136II GAGCTC 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 152
EcoRII CCWGG 2 cut(s) 37, 254
EcoT38I GRGCYC 1 cut(s) 154
FaeI CATG 1 cut(s) 161
FaiI YATR 5 cut(s) 56, 67, 159, 177, 207
FatI CATG 1 cut(s) 157
FauI CCCGC 1 cut(s) 243
FokI GGATG 3 cut(s) 218, 256, 271
FriOI GRGCYC 1 cut(s) 154
FspBI CTAG 1 cut(s) 30
GlaI GCGC 2 cut(s) 285, 357
HaeII RGCGCY 2 cut(s) 287, 359
HapII CCGG 1 cut(s) 409
HhaI GCGC 2 cut(s) 286, 358
Hin1II CATG 1 cut(s) 161
Hin6I GCGC 2 cut(s) 284, 356
HinP1I GCGC 2 cut(s) 284, 356
HpaII CCGG 1 cut(s) 409
HphI GGTGA 2 cut(s) 212, 346
Hpy188I TCNGA 2 cut(s) 104, 306
Hpy188III TCNNGA 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 19, 177
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 283
HpyF3I CTNAG 2 cut(s) 239, 287
Hsp92II CATG 1 cut(s) 161
HspAI GCGC 2 cut(s) 284, 356
Kzo9I GATC 4 cut(s) 106, 127, 139, 346
LguI GCTCTTC 2 cut(s) 159, 437
LweI GCATC 2 cut(s) 177, 417
MaeI CTAG 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 8
MalI GATC 4 cut(s) 108, 129, 141, 348
MboI GATC 4 cut(s) 106, 127, 139, 346
MboII GAAGA 5 cut(s) 146, 174, 386, 424, 435
MhlI GDGCHC 2 cut(s) 53, 154
MluCI AATT 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 82, 204
MnlI CCTC 5 cut(s) 220, 234, 364, 405, 433
MroXI GAANNNNTTC 1 cut(s) 166
MseI TTAA 1 cut(s) 448
MspA1I CMGCKG 2 cut(s) 238, 352
MspI CCGG 1 cut(s) 409
MspR9I CCNGG 2 cut(s) 39, 256
MvaI CCWGG 2 cut(s) 39, 256
MwoI GCNNNNNNNGC 2 cut(s) 48, 283
NdeII GATC 4 cut(s) 106, 127, 139, 346
NlaIII CATG 1 cut(s) 161
NlaIV GGNNCC 2 cut(s) 52, 319
NmuCI GTSAC 1 cut(s) 8
NspV TTCGAA 1 cut(s) 418
PciSI GCTCTTC 2 cut(s) 159, 437
PdmI GAANNNNTTC 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 37, 254
PspGI CCWGG 2 cut(s) 37, 254
PspN4I GGNNCC 2 cut(s) 52, 319
PspPI GGNCC 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 352
SacI GAGCTC 1 cut(s) 154
SapI GCTCTTC 2 cut(s) 159, 437
SaqAI TTAA 1 cut(s) 448
Sau3AI GATC 4 cut(s) 106, 127, 139, 346
Sau96I GGNCC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 39, 256
SduI GDGCHC 2 cut(s) 53, 154
SfaNI GCATC 2 cut(s) 177, 417
SfuI TTCGAA 1 cut(s) 418
SinI GGWCC 1 cut(s) 20
Sse9I AATT 1 cut(s) 122
SsiI CCGC 1 cut(s) 236
SspMI CTAG 1 cut(s) 30
SstI GAGCTC 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 37, 254
TaqI TCGA 4 cut(s) 148, 345, 418, 425
TaqII GACCGA 1 cut(s) 37
TasI AATT 1 cut(s) 122
Tru1I TTAA 1 cut(s) 448
Tru9I TTAA 1 cut(s) 448
TseFI GTSAC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 20
XmnI GAANNNNTTC 1 cut(s) 166
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.