Rmu_sc0000210.1_g000013

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000210.1
OG0020610
Physical Location & Seq
Forward (+)
62588 .. 66079
3492 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1257 bp
atggcaaaaatccccagcggggcgaaactccattggatagccgcccctaatagggggaacattcagggacaaatggggagtggggatggggatccccaatctccactatctaagattggggatggggtggggatgaaatatttgaagcataactggatcccgaatgtatacctttactacccatcttctccatcctttcgagatgcccctagatttgttgcggagcttattgatcctgttactcaaacttgggatttaaatatcattcagaggcgtttcaatcaagatgacattgagttgatactccgcataccgctaagtcggtctacggatcccttaaggttggtgtggaaggcaatttggactgctacaatacccggtaaggtggctttgcatgcatggagattggtaagcaatatctttcctactcgggttagccttagtactaaaggctataatggtgctttggattatagtttatgtgctcataattatgaagatagtttgcatttgttcatcaattgcctacatgcacgtgaggtgtggaatagagttggtgtcagctggcagggaccggccattgcttctattcaagagtggtttctggccatagtaccgacactatctaaagaagagctaaacaaggttctgatgttgctttggggaatatggaagaataggaatgcccaagtgtgggagcagaaaggtaaacacgcgtctgaaaccttgttgttaactgttggatggtatgaagagtttaagaagtataatgctagccctcacagacaaagccagcaacctgcacgttggaaggctccagcaataggtaggctatggtcttgtcgcggtgaggtcagtgcaaccgggcggggagtacgtttttctcactcatccgatgaggcttttaggttggacattgttgtggtcaaatgcttggctcgtttcggatccggttggattcgttttggggaggagttcatgcgctcagggctgctagcttggatttcaaggggaggtggagaggctgtgtgttctgtcaggaggggattccatggggagcgagtttggcggctcggatcttcatggattctaggatccggtttaggcgtgtatgtagctttcttcaatgtgggcggcaatggtgcttcattggcaggtgggtgtcagactgggtcgatggcggaggatactacggcggcgttggtgtgggtttctggggtctgtcatcttcatcgaagcattggcttgggattctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

418

Amino Acids

46.59

Weight (kDa)

9.57

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 115 - 182 3.1e-11 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020610)

Species Orthologous Gene IDs
rosa_laevigata RLG00000003429 RLG00000007759
rosa_multiflora Rmu_sc0000210.1_g000013
rosa_samantha Rh2DG285300 Rh7AG216400 Rh7CG229900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1145
Acc36I ACCTGC 2 cut(s) 808, 1145
AccI GTMKAC 2 cut(s) 168, 326
AccII CGCG 2 cut(s) 716, 846
AcoI YGGCCR 2 cut(s) 576, 606
AcvI CACGTG 1 cut(s) 536
AfaI GTAC 3 cut(s) 445, 615, 876
AfiI CCNNNNNNNGG 7 cut(s) 19, 51, 52, 53, 693, 694, 824
AflII CTTAAG 1 cut(s) 337
AflIII ACRYGT 1 cut(s) 714
AgsI TTSAA 5 cut(s) 145, 280, 593, 1008, 1126
AluBI AGCT 5 cut(s) 226, 564, 637, 998, 1118
AluI AGCT 5 cut(s) 226, 564, 637, 998, 1118
Alw21I GWGCWC 1 cut(s) 487
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 2 cut(s) 576, 606
ApeKI GCWGC 1 cut(s) 991
AspLEI GCGC 1 cut(s) 984
AspS9I GGNCC 1 cut(s) 572
AsuC2I CCSGG 2 cut(s) 378, 865
AsuHPI GGTGA 1 cut(s) 860
AsuNHI GCTAGC 2 cut(s) 773, 994
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 1 cut(s) 572
BalI TGGCCA 1 cut(s) 608
BamHI GGATCC 5 cut(s) 91, 156, 331, 947, 1094
BbrPI CACGTG 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 1 cut(s) 978
BccI CCATC 6 cut(s) 80, 116, 190, 199, 738, 1171
BceAI ACGGC 1 cut(s) 1209
BciVI GTATCC 1 cut(s) 1180
BcnI CCSGG 2 cut(s) 378, 865
BfaI CTAG 5 cut(s) 210, 774, 995, 1091, 1255
BfrI CTTAAG 1 cut(s) 337
BfuAI ACCTGC 2 cut(s) 808, 1145
BfuI GTATCC 1 cut(s) 1180
BisI GCNGC 5 cut(s) 42, 992, 1070, 1135, 1197
BlsI GCNGC 5 cut(s) 43, 993, 1071, 1136, 1198
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 2 cut(s) 378, 865
Bme18I GGWCC 1 cut(s) 572
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 7 cut(s) 93, 158, 333, 573, 816, 949, 1096
BmrFI CCNGG 2 cut(s) 378, 865
BmrI ACTGGG 1 cut(s) 1179
BmsI GCATC 1 cut(s) 193
BmtI GCTAGC 2 cut(s) 777, 998
BmuI ACTGGG 1 cut(s) 1179
BpmI CTGGAG 1 cut(s) 801
Bpu10I CCTNAGC 1 cut(s) 985
BpuMI CCSGG 2 cut(s) 378, 865
BsaAI YACGTR 1 cut(s) 536
BsaBI GATNNNNATC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 1051
BsaWI WCCGGW 2 cut(s) 950, 1097
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 51, 52, 53, 693, 694, 824
Bse118I RCCGGY 1 cut(s) 574
Bse1I ACTGG 2 cut(s) 158, 1174
Bse3DI GCAATG 2 cut(s) 579, 1144
Bse8I GATNNNNATC 1 cut(s) 90
BseDI CCNNGG 1 cut(s) 1051
BseGI GGATG 6 cut(s) 91, 127, 138, 191, 749, 890
BseJI GATNNNNATC 1 cut(s) 90
BseLI CCNNNNNNNGG 7 cut(s) 19, 51, 52, 53, 693, 694, 824
BseMI GCAATG 2 cut(s) 579, 1144
BseMII CTCAG 1 cut(s) 999
BseNI ACTGG 2 cut(s) 158, 1174
BseRI GAGGAG 1 cut(s) 986
BseXI GCAGC 1 cut(s) 978
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 786
Bsh1236I CGCG 2 cut(s) 716, 846
BshFI GGCC 2 cut(s) 578, 608
BsiHKAI GWGCWC 1 cut(s) 487
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 5 cut(s) 378, 575, 864, 951, 1098
BslFI GGGAC 2 cut(s) 81, 585
BslI CCNNNNNNNGG 7 cut(s) 19, 51, 52, 53, 693, 694, 824
BsmFI GGGAC 2 cut(s) 81, 585
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 2 cut(s) 578, 608
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
Bsp19I CCATGG 1 cut(s) 1051
BspANI GGCC 2 cut(s) 578, 608
BspCNI CTCAG 1 cut(s) 998
BspFNI CGCG 2 cut(s) 716, 846
BspLI GGNNCC 7 cut(s) 93, 158, 333, 573, 816, 949, 1096
BspMI ACCTGC 2 cut(s) 808, 1145
BspOI GCTAGC 2 cut(s) 777, 998
BspQI GCTCTTC 1 cut(s) 627
BspTI CTTAAG 1 cut(s) 337
BsrDI GCAATG 2 cut(s) 579, 1144
BsrFI RCCGGY 1 cut(s) 574
BsrI ACTGG 2 cut(s) 158, 1174
BssAI RCCGGY 1 cut(s) 574
BssECI CCNNGG 1 cut(s) 1051
BssMI GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
BssNAI GTATAC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 1051
Bst1107I GTATAC 1 cut(s) 169
Bst4CI ACNGT 1 cut(s) 739
Bst6I CTCTTC 2 cut(s) 627, 747
BstAFI CTTAAG 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 536
BstC8I GCNNGC 5 cut(s) 396, 566, 775, 794, 996
BstDEI CTNAG 4 cut(s) 111, 317, 440, 985
BstDSI CCRYGG 1 cut(s) 1051
BstF5I GGATG 6 cut(s) 91, 127, 138, 191, 749, 890
BstFNI CGCG 2 cut(s) 716, 846
BstHHI GCGC 1 cut(s) 984
BstKTI GATC 7 cut(s) 94, 159, 235, 334, 950, 1079, 1097
BstMBI GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
BstMWI GCNNNNNNNGC 4 cut(s) 395, 988, 1066, 1151
BstNSI RCATGY 2 cut(s) 398, 533
BstSCI CCNGG 2 cut(s) 376, 863
BstUI CGCG 2 cut(s) 716, 846
BstV1I GCAGC 1 cut(s) 978
BstX2I RGATCY 6 cut(s) 91, 156, 331, 947, 1076, 1094
BstYI RGATCY 6 cut(s) 91, 156, 331, 947, 1076, 1094
BstZ17I GTATAC 1 cut(s) 169
BsuI GTATCC 1 cut(s) 1180
BsuRI GGCC 2 cut(s) 578, 608
BtgI CCRYGG 1 cut(s) 1051
BtsCI GGATG 6 cut(s) 91, 127, 138, 191, 749, 890
BtsIMutI CAGTG 1 cut(s) 862
BveI ACCTGC 2 cut(s) 808, 1145
Cac8I GCNNGC 5 cut(s) 396, 566, 775, 794, 996
CfoI GCGC 1 cut(s) 984
Cfr10I RCCGGY 1 cut(s) 574
Cfr13I GGNCC 1 cut(s) 572
CseI GACGC 1 cut(s) 705
Csp6I GTAC 3 cut(s) 444, 614, 875
CviAII CATG 6 cut(s) 395, 399, 530, 979, 1052, 1083
CviQI GTAC 3 cut(s) 444, 614, 875
DdeI CTNAG 4 cut(s) 111, 317, 440, 985
DpnI GATC 7 cut(s) 93, 158, 234, 333, 949, 1078, 1096
DpnII GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
DraI TTTAAA 1 cut(s) 258
EaeI YGGCCR 2 cut(s) 576, 606
Eam1104I CTCTTC 2 cut(s) 627, 747
EarI CTCTTC 2 cut(s) 627, 747
EciI GGCGGA 1 cut(s) 1196
Eco130I CCWWGG 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 572
Eco72I CACGTG 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 1051
EcoT22I ATGCAT 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 1051
FaeI CATG 6 cut(s) 398, 402, 533, 982, 1055, 1086
FaqI GGGAC 2 cut(s) 81, 585
FatI CATG 6 cut(s) 394, 398, 529, 978, 1051, 1082
FauI CCCGC 2 cut(s) 11, 861
FblI GTMKAC 2 cut(s) 168, 326
Fnu4HI GCNGC 5 cut(s) 42, 992, 1070, 1135, 1197
FokI GGATG 6 cut(s) 98, 134, 145, 178, 756, 877
Fsp4HI GCNGC 5 cut(s) 42, 992, 1070, 1135, 1197
FspBI CTAG 5 cut(s) 210, 774, 995, 1091, 1255
GlaI GCGC 1 cut(s) 983
GluI GCNGC 5 cut(s) 42, 992, 1070, 1135, 1197
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 2 cut(s) 578, 608
HapII CCGG 5 cut(s) 378, 575, 864, 951, 1098
HgaI GACGC 1 cut(s) 705
HhaI GCGC 1 cut(s) 984
Hin1II CATG 6 cut(s) 398, 402, 533, 982, 1055, 1086
Hin6I GCGC 1 cut(s) 982
HinP1I GCGC 1 cut(s) 982
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 4 cut(s) 958, 1047, 1087, 1251
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 5 cut(s) 378, 575, 864, 951, 1098
HphI GGTGA 1 cut(s) 860
Hpy166II GTNNAC 4 cut(s) 169, 327, 710, 735
Hpy188I TCNGA 7 cut(s) 270, 651, 721, 895, 947, 1076, 1167
Hpy188III TCNNGA 5 cut(s) 160, 200, 284, 593, 1039
Hpy8I GTNNAC 4 cut(s) 169, 327, 710, 735
HpyAV CCTTC 2 cut(s) 346, 805
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4IV ACGT 3 cut(s) 535, 805, 877
HpyCH4V TGCA 6 cut(s) 394, 398, 508, 533, 803, 860
HpyF10VI GCNNNNNNNGC 4 cut(s) 395, 988, 1066, 1151
HpyF3I CTNAG 4 cut(s) 111, 317, 440, 985
HpySE526I ACGT 3 cut(s) 535, 805, 877
Hsp92II CATG 6 cut(s) 398, 402, 533, 982, 1055, 1086
HspAI GCGC 1 cut(s) 982
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 4 cut(s) 223, 697, 820, 1057
Lsp1109I GCAGC 1 cut(s) 978
LweI GCATC 1 cut(s) 193
MaeI CTAG 5 cut(s) 210, 774, 995, 1091, 1255
MaeII ACGT 3 cut(s) 535, 805, 877
MaeIII GTNAC 1 cut(s) 238
MalI GATC 7 cut(s) 93, 158, 234, 333, 949, 1078, 1096
MboI GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
MboII GAAGA 8 cut(s) 177, 509, 644, 685, 764, 1071, 1114, 1220
MfeI CAATTG 1 cut(s) 520
MflI RGATCY 6 cut(s) 91, 156, 331, 947, 1076, 1094
MhlI GDGCHC 1 cut(s) 487
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 3 cut(s) 357, 490, 520
MluI ACGCGT 1 cut(s) 714
MluNI TGGCCA 1 cut(s) 608
MmeI TCCRAC 4 cut(s) 721, 788, 891, 935
Mox20I TGGCCA 1 cut(s) 608
Mph1103I ATGCAT 1 cut(s) 400
MscI TGGCCA 1 cut(s) 608
MseI TTAA 4 cut(s) 257, 338, 734, 759
MslI CAYNNNNRTG 3 cut(s) 492, 534, 920
Msp20I TGGCCA 1 cut(s) 608
MspA1I CMGCKG 2 cut(s) 18, 564
MspCI CTTAAG 1 cut(s) 337
MspI CCGG 5 cut(s) 378, 575, 864, 951, 1098
MspR9I CCNGG 2 cut(s) 378, 865
MunI CAATTG 1 cut(s) 520
Mva1269I GAATGC 1 cut(s) 688
MvnI CGCG 2 cut(s) 716, 846
MwoI GCNNNNNNNGC 4 cut(s) 395, 988, 1066, 1151
NciI CCSGG 2 cut(s) 378, 865
NcoI CCATGG 1 cut(s) 1051
NdeII GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
NheI GCTAGC 2 cut(s) 773, 994
NlaIII CATG 6 cut(s) 398, 402, 533, 982, 1055, 1086
NlaIV GGNNCC 7 cut(s) 93, 158, 333, 573, 816, 949, 1096
NsiI ATGCAT 1 cut(s) 400
NspI RCATGY 2 cut(s) 398, 533
PaeI GCATGC 1 cut(s) 398
PaqCI CACCTGC 1 cut(s) 1145
PciSI GCTCTTC 1 cut(s) 627
PctI GAATGC 1 cut(s) 688
PfeI GAWTC 4 cut(s) 958, 1047, 1087, 1251
PflFI GACNNNGTC 1 cut(s) 1171
PkrI GCNGC 5 cut(s) 43, 993, 1071, 1136, 1198
PmaCI CACGTG 1 cut(s) 536
PmlI CACGTG 1 cut(s) 536
Ppu21I YACGTR 1 cut(s) 536
PspCI CACGTG 1 cut(s) 536
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 7 cut(s) 93, 158, 333, 573, 816, 949, 1096
PspPI GGNCC 1 cut(s) 572
PsuI RGATCY 6 cut(s) 91, 156, 331, 947, 1076, 1094
PsyI GACNNNGTC 1 cut(s) 1171
PvuII CAGCTG 1 cut(s) 564
RsaI GTAC 3 cut(s) 445, 615, 876
RsaNI GTAC 3 cut(s) 444, 614, 875
RseI CAYNNNNRTG 3 cut(s) 492, 534, 920
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 4 cut(s) 257, 338, 734, 759
SatI GCNGC 5 cut(s) 42, 992, 1070, 1135, 1197
Sau3AI GATC 7 cut(s) 91, 156, 232, 331, 947, 1076, 1094
Sau96I GGNCC 1 cut(s) 572
ScaI AGTACT 1 cut(s) 445
ScrFI CCNGG 2 cut(s) 378, 865
SduI GDGCHC 1 cut(s) 487
SfaNI GCATC 1 cut(s) 193
SinI GGWCC 1 cut(s) 572
SmiI ATTTAAAT 1 cut(s) 258
SmiMI CAYNNNNRTG 3 cut(s) 492, 534, 920
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
SphI GCATGC 1 cut(s) 398
Sse9I AATT 3 cut(s) 357, 490, 520
SspI AATATT 1 cut(s) 140
SspMI CTAG 5 cut(s) 210, 774, 995, 1091, 1255
StyD4I CCNGG 2 cut(s) 376, 863
StyI CCWWGG 1 cut(s) 1051
SwaI ATTTAAAT 1 cut(s) 258
TaaI ACNGT 1 cut(s) 739
TaiI ACGT 3 cut(s) 538, 808, 880
TaqI TCGA 3 cut(s) 199, 1175, 1234
TaqII GACCGA 1 cut(s) 312
TasI AATT 3 cut(s) 357, 490, 520
TatI WGTACW 1 cut(s) 443
TauI GCSGC 4 cut(s) 44, 1072, 1137, 1199
TfiI GAWTC 4 cut(s) 958, 1047, 1087, 1251
Tru1I TTAA 4 cut(s) 257, 338, 734, 759
Tru9I TTAA 4 cut(s) 257, 338, 734, 759
TscAI CASTG 1 cut(s) 862
TseI GCWGC 1 cut(s) 991
TspDTI ATGAA 8 cut(s) 149, 505, 510, 765, 967, 1071, 1137, 1220
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 1 cut(s) 862
Tth111I GACNNNGTC 1 cut(s) 1171
Vha464I CTTAAG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 572
XceI RCATGY 2 cut(s) 398, 533
XmiI GTMKAC 2 cut(s) 168, 326
XspI CTAG 5 cut(s) 210, 774, 995, 1091, 1255
ZrmI AGTACT 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.