Rmu_sc0000266.1_g000004

Transaldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000266.1
OG0015990
Physical Location & Seq
Reverse (-)
15117 .. 20110
4994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000266.1_g000004.1.cds

Sequence Viewer

Length: 1170 bp
atgtcagtctccttgcaatctccgccttcctcagctatctcctctttccagggaagaaagctgagcttctctaatggatccgcaccttccaatctgctcttcacctcccgcagatcacttcctctcattcgcgcttccgctgtcgacactggccccgctactgaattggacgccgtatctagcttcagtgagatcgttcctgacaccgtcattttcgatgacttcgagaggtttcctccaactgctgctactgtgagctccgccctactcttgggtatatgcagtcttcctgataccatatttagaaatgctgtggatatggcgctggcagattcggaatgtaataagcatgagaattccgaattgagactgtcatgtttttgtagcaaggctttagtcaatgttggtgctgatttggtaaaactagttcctggccgagtgtccactgaggtggatgcacgtcttgcttatgacacgcatggaattataaggaaggtgcacgatctgctgaagctgtatgatagagttgatgttcctcccgagcgtttgctgttcaaaattccttcaacttggcaaggaatagaggcttcaagattactagaatctgagggcatacagacgcatttgacatttgtgtacagctttgctcaagctgcagctgccgcacaagccggcgcttctgttattcagatttttgttgggcgtcttagggattggtcccgtaaccattctggtgaccctgagatagaggctgctctcaaaagaggagaggatcctgggttggccctggtgacaaagacatataactacatccacaaatatggacataagtcaaagttgatggcagcagcagttcgcaataagcaggacttattcagtctcctgggtgtcgactacatcattgcaccactgaaggtattgcaatcactaaaagaaacagtcactactcctgaagagaagtactcttttgtcaggaggctatctccacaatctgctgccaagtacaatttcaatgaagtagagcttgtgaagtgggaccaattaacatttgcatcagccgtgggacctgcagctgtggagcttctggccggtggactagatggttacgcgaatcaagcaaaccgagttgaggaattatttggaaaactctggccacctccaaatgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00030 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00030 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TAL_FSA

Protein Analysis

389

Amino Acids

42.47

Weight (kDa)

6.2

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Orthologous Genes (Group: OG0015990)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12230 AT1G12230
fragaria_vesca FvH4_5g00280
malus_domestica MD06G1067000.v1.1
prunus_persica Prupe.5G075500_v2.0.a1
pyrus_communis pycom06g05960
rosa_chinensis RchiOBHm_Chr7g0202331
rosa_laevigata RLG00000003608
rosa_multiflora Rmu_sc0000266.1_g000004
rosa_roxburghii Rroxscaffold_3G00254660
rosa_rugosa Rorug07G0069900
rosa_samantha Rh7BG198400 Rh7CG208400 Rh7DG204100
rosa_wichuraiana Rw7G017290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 488
Acc36I ACCTGC 1 cut(s) 1075
AccI GTMKAC 2 cut(s) 144, 891
AccII CGCG 2 cut(s) 132, 1109
AciI CCGC 7 cut(s) 23, 81, 109, 138, 156, 261, 663
AclWI GGATC 4 cut(s) 72, 85, 767, 780
AcoI YGGCCR 3 cut(s) 433, 1086, 1151
AcsI RAATTY 2 cut(s) 355, 558
AcuI CTGAAG 4 cut(s) 169, 530, 932, 972
AcyI GRCGYC 2 cut(s) 171, 703
AfaI GTAC 3 cut(s) 638, 962, 1004
AfiI CCNNNNNNNGG 2 cut(s) 271, 1129
AgsI TTSAA 4 cut(s) 556, 567, 591, 1012
AhlI ACTAGT 1 cut(s) 424
AjiI CACGTC 1 cut(s) 461
AjnI CCWGG 5 cut(s) 48, 430, 775, 786, 882
AjuI GAANNNNNNNTTGG 4 cut(s) 83, 115, 992, 1024
AleI CACNNNNGTG 1 cut(s) 449
Alw21I GWGCWC 2 cut(s) 260, 501
Alw26I GTCTC 3 cut(s) 13, 361, 884
Alw44I GTGCAC 1 cut(s) 497
AlwI GGATC 4 cut(s) 72, 85, 767, 780
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 5 cut(s) 151, 433, 783, 1086, 1151
ApaLI GTGCAC 1 cut(s) 497
ApeKI GCWGC 9 cut(s) 245, 653, 656, 659, 752, 845, 848, 995, 1070
ApoI RAATTY 2 cut(s) 355, 558
AspLEI GCGC 3 cut(s) 134, 325, 677
AspS9I GGNCC 5 cut(s) 152, 717, 784, 1036, 1064
AsuHPI GGTGA 3 cut(s) 94, 746, 802
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 3 cut(s) 717, 1036, 1064
BaeGI GKGCMC 1 cut(s) 501
BalI TGGCCA 1 cut(s) 1153
BamHI GGATCC 2 cut(s) 77, 772
BanII GRGCYC 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 2 cut(s) 260, 501
BbvCI CCTCAGC 1 cut(s) 31
BbvI GCAGC 9 cut(s) 232, 640, 646, 668, 739, 857, 860, 982, 1082
BccI CCATC 2 cut(s) 835, 1094
BceAI ACGGC 2 cut(s) 158, 1043
BciT130I CCWGG 5 cut(s) 50, 432, 777, 788, 884
BcoDI GTCTC 3 cut(s) 13, 361, 884
BcuI ACTAGT 1 cut(s) 424
BfaI CTAG 4 cut(s) 180, 425, 599, 1097
BfmI CTRYAG 2 cut(s) 654, 1068
BfoI RGCGCY 2 cut(s) 326, 678
BfuAI ACCTGC 1 cut(s) 1075
BlpI GCTNAGC 1 cut(s) 62
BmcAI AGTACT 1 cut(s) 962
Bme1390I CCNGG 5 cut(s) 50, 432, 777, 788, 884
Bme18I GGWCC 3 cut(s) 717, 1036, 1064
BmeT110I CYCGRG 1 cut(s) 539
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 5 cut(s) 152, 717, 784, 1036, 1064
BmiI GGNNCC 6 cut(s) 79, 154, 719, 774, 1037, 1065
BmrFI CCNGG 5 cut(s) 50, 432, 777, 788, 884
BmsI GCATC 2 cut(s) 445, 1061
BoxI GACNNNNGTC 1 cut(s) 829
BpiI GAAGAC 1 cut(s) 278
BplI GAGNNNNNCTC 6 cut(s) 220, 252, 947, 979, 967, 999
Bpu10I CCTNAGC 1 cut(s) 31
Bpu1102I GCTNAGC 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 633
BsaHI GRCGYC 2 cut(s) 171, 703
BsaJI CCNNGG 5 cut(s) 49, 776, 786, 883, 1059
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 1129
Bse118I RCCGGY 2 cut(s) 671, 1088
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 900
BseBI CCWGG 5 cut(s) 50, 432, 777, 788, 884
BseDI CCNNGG 5 cut(s) 49, 776, 786, 883, 1059
BseGI GGATG 2 cut(s) 460, 810
BseLI CCNNNNNNNGG 2 cut(s) 271, 1129
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 5 cut(s) 45, 53, 438, 597, 732
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 2 cut(s) 31, 780
BseSI GKGCMC 1 cut(s) 501
BseXI GCAGC 9 cut(s) 232, 640, 646, 668, 739, 857, 860, 982, 1082
Bsh1236I CGCG 2 cut(s) 132, 1109
BshFI GGCC 5 cut(s) 153, 435, 785, 1088, 1153
BsiHKAI GWGCWC 2 cut(s) 260, 501
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 672, 1089
BslFI GGGAC 3 cut(s) 703, 1049, 1077
BslI CCNNNNNNNGG 2 cut(s) 271, 1129
BsmAI GTCTC 3 cut(s) 13, 361, 884
BsmFI GGGAC 3 cut(s) 703, 1049, 1077
BsnI GGCC 5 cut(s) 153, 435, 785, 1088, 1153
BsoBI CYCGRG 1 cut(s) 539
Bsp1286I GDGCHC 2 cut(s) 260, 501
Bsp1407I TGTACA 1 cut(s) 636
Bsp143I GATC 5 cut(s) 77, 113, 192, 502, 772
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 7 cut(s) 23, 81, 109, 138, 156, 261, 663
BspANI GGCC 5 cut(s) 153, 435, 785, 1088, 1153
BspCNI CTCAG 5 cut(s) 44, 54, 439, 598, 733
BspFNI CGCG 2 cut(s) 132, 1109
BspLI GGNNCC 6 cut(s) 79, 154, 719, 774, 1037, 1065
BspMAI CTGCAG 2 cut(s) 658, 1072
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 4 cut(s) 72, 85, 767, 780
BspQI GCTCTTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 900
BsrFI RCCGGY 2 cut(s) 671, 1088
BsrGI TGTACA 1 cut(s) 636
BsrI ACTGG 1 cut(s) 154
BssAI RCCGGY 2 cut(s) 671, 1088
BssECI CCNNGG 5 cut(s) 49, 776, 786, 883, 1059
BssMI GATC 5 cut(s) 77, 113, 192, 502, 772
BssNI GRCGYC 2 cut(s) 171, 703
Bst2UI CCWGG 5 cut(s) 50, 432, 777, 788, 884
Bst4CI ACNGT 4 cut(s) 208, 253, 372, 940
Bst6I CTCTTC 2 cut(s) 104, 948
BstACI GRCGYC 2 cut(s) 171, 703
BstAPI GCANNNNNTGC 2 cut(s) 464, 505
BstAUI TGTACA 1 cut(s) 636
BstC8I GCNNGC 2 cut(s) 327, 673
BstDEI CTNAG 6 cut(s) 31, 62, 447, 606, 707, 741
BstDSI CCRYGG 1 cut(s) 1059
BstEII GGTNACC 1 cut(s) 734
BstF5I GGATG 2 cut(s) 460, 810
BstFNI CGCG 2 cut(s) 132, 1109
BstH2I RGCGCY 2 cut(s) 326, 678
BstHHI GCGC 3 cut(s) 134, 325, 677
BstKTI GATC 5 cut(s) 80, 116, 195, 505, 775
BstMAI GTCTC 3 cut(s) 13, 361, 884
BstMBI GATC 5 cut(s) 77, 113, 192, 502, 772
BstMWI GCNNNNNNNGC 8 cut(s) 22, 464, 505, 653, 659, 662, 668, 1115
BstNI CCWGG 5 cut(s) 50, 432, 777, 788, 884
BstPAI GACNNNNGTC 1 cut(s) 829
BstPI GGTNACC 1 cut(s) 734
BstSCI CCNGG 5 cut(s) 48, 430, 775, 786, 882
BstSFI CTRYAG 2 cut(s) 654, 1068
BstSLI GKGCMC 1 cut(s) 501
BstUI CGCG 2 cut(s) 132, 1109
BstV1I GCAGC 9 cut(s) 232, 640, 646, 668, 739, 857, 860, 982, 1082
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 2 cut(s) 77, 772
BstXI CCANNNNNNTGG 2 cut(s) 451, 821
BstYI RGATCY 2 cut(s) 77, 772
BsuRI GGCC 5 cut(s) 153, 435, 785, 1088, 1153
BtgI CCRYGG 1 cut(s) 1059
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 2 cut(s) 460, 810
BtsIMutI CAGTG 4 cut(s) 147, 193, 444, 908
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 2 cut(s) 327, 673
CfoI GCGC 3 cut(s) 134, 325, 677
Cfr10I RCCGGY 2 cut(s) 671, 1088
Cfr13I GGNCC 5 cut(s) 152, 717, 784, 1036, 1064
CseI GACGC 3 cut(s) 179, 628, 692
Csp6I GTAC 3 cut(s) 637, 961, 1003
CviAII CATG 3 cut(s) 350, 375, 479
CviQI GTAC 3 cut(s) 637, 961, 1003
DdeI CTNAG 6 cut(s) 31, 62, 447, 606, 707, 741
DpnI GATC 5 cut(s) 79, 115, 194, 504, 774
DpnII GATC 5 cut(s) 77, 113, 192, 502, 772
EaeI YGGCCR 3 cut(s) 433, 1086, 1151
Eam1104I CTCTTC 2 cut(s) 104, 948
EarI CTCTTC 2 cut(s) 104, 948
EciI GGCGGA 2 cut(s) 12, 250
Ecl136II GAGCTC 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 260
Eco47I GGWCC 3 cut(s) 717, 1036, 1064
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 4 cut(s) 169, 530, 932, 972
Eco88I CYCGRG 1 cut(s) 539
Eco91I GGTNACC 1 cut(s) 734
EcoICRI GAGCTC 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 1064
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 1 cut(s) 355
EcoRII CCWGG 5 cut(s) 48, 430, 775, 786, 882
EcoT38I GRGCYC 1 cut(s) 260
FaeI CATG 3 cut(s) 353, 378, 482
FalI AAGNNNNNCTT 6 cut(s) 50, 82, 854, 886, 1008, 1040
FaqI GGGAC 3 cut(s) 703, 1049, 1077
FatI CATG 3 cut(s) 349, 374, 478
FauI CCCGC 2 cut(s) 116, 163
FblI GTMKAC 2 cut(s) 144, 891
FokI GGATG 2 cut(s) 467, 797
FriOI GRGCYC 1 cut(s) 260
FspBI CTAG 4 cut(s) 180, 425, 599, 1097
GlaI GCGC 3 cut(s) 133, 324, 676
HaeII RGCGCY 2 cut(s) 326, 678
HaeIII GGCC 5 cut(s) 153, 435, 785, 1088, 1153
HapII CCGG 2 cut(s) 672, 1089
HgaI GACGC 3 cut(s) 179, 628, 692
HhaI GCGC 3 cut(s) 134, 325, 677
Hin1I GRCGYC 2 cut(s) 171, 703
Hin1II CATG 3 cut(s) 353, 378, 482
Hin6I GCGC 3 cut(s) 132, 323, 675
HinP1I GCGC 3 cut(s) 132, 323, 675
HincII GTYRAC 2 cut(s) 145, 892
HindII GTYRAC 2 cut(s) 145, 892
HinfI GANTC 3 cut(s) 332, 602, 1111
HpaII CCGG 2 cut(s) 672, 1089
HphI GGTGA 3 cut(s) 94, 746, 802
Hpy166II GTNNAC 6 cut(s) 145, 444, 499, 637, 892, 1094
Hpy188I TCNGA 4 cut(s) 337, 361, 607, 690
Hpy188III TCNNGA 7 cut(s) 200, 226, 290, 539, 591, 950, 973
Hpy8I GTNNAC 6 cut(s) 145, 444, 499, 637, 892, 1094
HpyAV CCTTC 5 cut(s) 36, 96, 487, 573, 907
HpyCH4III ACNGT 4 cut(s) 208, 253, 372, 940
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 9 cut(s) 16, 282, 458, 499, 656, 905, 922, 1052, 1070
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 464, 505, 653, 659, 662, 668, 1115
HpyF3I CTNAG 6 cut(s) 31, 62, 447, 606, 707, 741
HpySE526I ACGT 1 cut(s) 460
Hsp92I GRCGYC 2 cut(s) 171, 703
Hsp92II CATG 3 cut(s) 353, 378, 482
HspAI GCGC 3 cut(s) 132, 323, 675
KroI GCCGGC 1 cut(s) 671
KroNI GCCGGC 1 cut(s) 673
Kzo9I GATC 5 cut(s) 77, 113, 192, 502, 772
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 2 cut(s) 263, 1078
Lsp1109I GCAGC 9 cut(s) 232, 640, 646, 668, 739, 857, 860, 982, 1082
LweI GCATC 2 cut(s) 445, 1061
MaeI CTAG 4 cut(s) 180, 425, 599, 1097
MaeII ACGT 1 cut(s) 460
MaeIII GTNAC 5 cut(s) 722, 734, 790, 940, 1103
MalI GATC 5 cut(s) 79, 115, 194, 504, 774
MboI GATC 5 cut(s) 77, 113, 192, 502, 772
MboII GAAGA 4 cut(s) 66, 91, 278, 965
MflI RGATCY 2 cut(s) 77, 772
MhlI GDGCHC 2 cut(s) 260, 501
MlsI TGGCCA 1 cut(s) 1153
MluCI AATT 8 cut(s) 164, 355, 362, 483, 558, 1006, 1040, 1133
MluNI TGGCCA 1 cut(s) 1153
MmeI TCCRAC 1 cut(s) 263
Mox20I TGGCCA 1 cut(s) 1153
MroNI GCCGGC 1 cut(s) 671
MscI TGGCCA 1 cut(s) 1153
MseI TTAA 1 cut(s) 1043
MslI CAYNNNNRTG 3 cut(s) 449, 732, 819
Msp20I TGGCCA 1 cut(s) 1153
MspA1I CMGCKG 3 cut(s) 140, 659, 1073
MspI CCGG 2 cut(s) 672, 1089
MspR9I CCNGG 5 cut(s) 50, 432, 777, 788, 884
MvaI CCWGG 5 cut(s) 50, 432, 777, 788, 884
MvnI CGCG 2 cut(s) 132, 1109
MwoI GCNNNNNNNGC 8 cut(s) 22, 464, 505, 653, 659, 662, 668, 1115
NaeI GCCGGC 1 cut(s) 673
NdeII GATC 5 cut(s) 77, 113, 192, 502, 772
NgoMIV GCCGGC 1 cut(s) 671
NlaIII CATG 3 cut(s) 353, 378, 482
NlaIV GGNNCC 6 cut(s) 79, 154, 719, 774, 1037, 1065
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 3 cut(s) 734, 790, 940
OliI CACNNNNGTG 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 673
PfeI GAWTC 3 cut(s) 332, 602, 1111
PflFI GACNNNGTC 1 cut(s) 206
PpuMI RGGWCCY 1 cut(s) 1064
PshAI GACNNNNGTC 1 cut(s) 829
PsiI TTATAA 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 1064
Psp6I CCWGG 5 cut(s) 48, 430, 775, 786, 882
PspEI GGTNACC 1 cut(s) 734
PspGI CCWGG 5 cut(s) 48, 430, 775, 786, 882
PspN4I GGNNCC 6 cut(s) 79, 154, 719, 774, 1037, 1065
PspPI GGNCC 5 cut(s) 152, 717, 784, 1036, 1064
PspPPI RGGWCCY 1 cut(s) 1064
PsrI GAACNNNNNNTAC 2 cut(s) 411, 443
PstI CTGCAG 2 cut(s) 658, 1072
PsuI RGATCY 2 cut(s) 77, 772
PsyI GACNNNGTC 1 cut(s) 206
PvuII CAGCTG 2 cut(s) 659, 1073
RsaI GTAC 3 cut(s) 638, 962, 1004
RsaNI GTAC 3 cut(s) 637, 961, 1003
RseI CAYNNNNRTG 3 cut(s) 449, 732, 819
SacI GAGCTC 1 cut(s) 260
SalI GTCGAC 2 cut(s) 143, 890
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 1043
Sau3AI GATC 5 cut(s) 77, 113, 192, 502, 772
Sau96I GGNCC 5 cut(s) 152, 717, 784, 1036, 1064
ScaI AGTACT 1 cut(s) 962
ScrFI CCNGG 5 cut(s) 50, 432, 777, 788, 884
SduI GDGCHC 2 cut(s) 260, 501
SfaNI GCATC 2 cut(s) 445, 1061
SfcI CTRYAG 2 cut(s) 654, 1068
SinI GGWCC 3 cut(s) 717, 1036, 1064
SmiMI CAYNNNNRTG 3 cut(s) 449, 732, 819
SmlI CTYRAG 1 cut(s) 648
SmoI CTYRAG 1 cut(s) 648
SpeI ACTAGT 1 cut(s) 424
Sse9I AATT 8 cut(s) 164, 355, 362, 483, 558, 1006, 1040, 1133
SsiI CCGC 7 cut(s) 23, 81, 109, 138, 156, 261, 663
SspMI CTAG 4 cut(s) 180, 425, 599, 1097
SstI GAGCTC 1 cut(s) 260
StyD4I CCNGG 5 cut(s) 48, 430, 775, 786, 882
TaaI ACNGT 4 cut(s) 208, 253, 372, 940
TaiI ACGT 1 cut(s) 463
TaqI TCGA 4 cut(s) 144, 216, 225, 891
TasI AATT 8 cut(s) 164, 355, 362, 483, 558, 1006, 1040, 1133
TatI WGTACW 3 cut(s) 636, 960, 1002
TauI GCSGC 1 cut(s) 665
TfiI GAWTC 3 cut(s) 332, 602, 1111
Tru1I TTAA 1 cut(s) 1043
Tru9I TTAA 1 cut(s) 1043
TscAI CASTG 4 cut(s) 154, 193, 451, 915
TseFI GTSAC 3 cut(s) 734, 790, 940
TseI GCWGC 9 cut(s) 245, 653, 656, 659, 752, 845, 848, 995, 1070
Tsp45I GTSAC 3 cut(s) 734, 790, 940
TspDTI ATGAA 1 cut(s) 1029
TspRI CASTG 4 cut(s) 154, 193, 451, 915
Tth111I GACNNNGTC 1 cut(s) 206
VneI GTGCAC 1 cut(s) 497
VpaK11BI GGWCC 3 cut(s) 717, 1036, 1064
XapI RAATTY 2 cut(s) 355, 558
XmiI GTMKAC 2 cut(s) 144, 891
XspI CTAG 4 cut(s) 180, 425, 599, 1097
ZrmI AGTACT 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.