Rmu_sc0000288.1_g000034

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000288.1
OG0023035
Physical Location & Seq
Forward (+)
112970 .. 113839
870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000288.1_g000034.1.cds

Sequence Viewer

Length: 870 bp
atggagctacctatacccagagaggtgaagaagatatgggacgtgtggaatctgcggggctgtattctcttaagtctctccctgcaagtttctctgcttttggcatcatttcggaaaaaatgcagaagcaaattgctacgaggactcatctggtctgcgtatttgctcgccgattggattgctgctgttgcaatagggataatcaccaaatcccaagttgacacttgcgacccgaagggcaacaaagatcttttggcattctgggcttcctttcttctactccatcttggcggccctgataccattacctcttttgctcttgaggacaatgagttctgggttaggcacttggttggactaattgttcaagttcttgcagctgcttattgtttctacctgacacttgttcataacaagttcttggttccgacggtgctagtctttgttgttggaacaatcaagtttggggagagaacttgtgctctgtatcttgcaagtatagaccattttggggagactgtattgccaaagccggtggccggacctgactatgaagaaactgtagcaatccacacttcaatgaggtcagttgaagtccctacaacacaagaagtagtggtgtcaatcaggccatgtattggggatgcacataaagatcccaagtttacagtcaaaggaaataaaaaagaatctttaaatgacatgcaattgctgcaagaagcatattacctctttgagagcttcaaaagactcattgttggcttccttctcagctccaaagaccgagaactgagtcgagatttcttccttaaaagaacctatactagcgcttttcagttgatagagtacgagctcaaaaccaacttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

289

Amino Acids

32.77

Weight (kDa)

8.44

Isoelectric Point (pI)

24.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023035)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000288.1_g000034 Rmu_sc0008085.1_g000020 Rmu_sc0009296.1_g000016
rosa_rugosa Rorug03G0157200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 638
AciI CCGC 2 cut(s) 55, 291
AclWI GGATC 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 537
AfaI GTAC 1 cut(s) 848
AfeI AGCGCT 1 cut(s) 829
AfiI CCNNNNNNNGG 3 cut(s) 511, 539, 638
AflII CTTAAG 1 cut(s) 70
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 4 cut(s) 368, 579, 593, 745
AjiI CACGTC 1 cut(s) 43
AloI GAACNNNNNNTCC 4 cut(s) 317, 348, 349, 380
AluBI AGCT 5 cut(s) 7, 380, 741, 774, 853
AluI AGCT 5 cut(s) 7, 380, 741, 774, 853
Alw21I GWGCWC 2 cut(s) 484, 855
Alw26I GTCTC 2 cut(s) 80, 509
AlwI GGATC 1 cut(s) 650
Aor51HI AGCGCT 1 cut(s) 829
AoxI GGCC 3 cut(s) 292, 537, 629
ApeKI GCWGC 4 cut(s) 182, 377, 380, 712
AspLEI GCGC 1 cut(s) 830
AspS9I GGNCC 2 cut(s) 293, 542
AsuHPI GGTGA 2 cut(s) 37, 196
AvaII GGWCC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 484, 855
BbvI GCAGC 4 cut(s) 169, 367, 389, 699
BccI CCATC 1 cut(s) 291
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BcoDI GTCTC 2 cut(s) 80, 509
BfaI CTAG 2 cut(s) 437, 825
BfmI CTRYAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 831
BfrI CTTAAG 1 cut(s) 70
BglII AGATCT 1 cut(s) 247
BisI GCNGC 5 cut(s) 183, 292, 378, 381, 713
BlsI GCNGC 5 cut(s) 184, 293, 379, 382, 714
Bme18I GGWCC 1 cut(s) 542
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 293, 542
BmiI GGNNCC 1 cut(s) 426
BmsI GCATC 2 cut(s) 113, 634
BpuEI CTTGAG 1 cut(s) 341
Bsc4I CCNNNNNNNGG 3 cut(s) 511, 539, 638
Bse118I RCCGGY 1 cut(s) 532
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 3 cut(s) 511, 539, 638
BseMII CTCAG 2 cut(s) 782, 784
BseXI GCAGC 4 cut(s) 169, 367, 389, 699
BshFI GGCC 3 cut(s) 294, 539, 631
BsiHKAI GWGCWC 2 cut(s) 484, 855
BsiSI CCGG 2 cut(s) 533, 540
BslFI GGGAC 2 cut(s) 53, 581
BslI CCNNNNNNNGG 3 cut(s) 511, 539, 638
BsmAI GTCTC 2 cut(s) 80, 509
BsmFI GGGAC 2 cut(s) 53, 581
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 3 cut(s) 294, 539, 631
Bsp1286I GDGCHC 2 cut(s) 484, 855
Bsp143I GATC 2 cut(s) 247, 655
BspACI CCGC 2 cut(s) 55, 291
BspANI GGCC 3 cut(s) 294, 539, 631
BspCNI CTCAG 2 cut(s) 783, 783
BspLI GGNNCC 1 cut(s) 426
BspPI GGATC 1 cut(s) 650
BspTI CTTAAG 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 532
BssAI RCCGGY 1 cut(s) 532
BssMI GATC 2 cut(s) 247, 655
Bst4CI ACNGT 4 cut(s) 433, 520, 562, 670
BstAFI CTTAAG 1 cut(s) 70
BstAPI GCANNNNNTGC 1 cut(s) 712
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 2 cut(s) 770, 791
BstF5I GGATG 1 cut(s) 649
BstH2I RGCGCY 1 cut(s) 831
BstHHI GCGC 1 cut(s) 830
BstKTI GATC 2 cut(s) 250, 658
BstMAI GTCTC 2 cut(s) 80, 509
BstMBI GATC 2 cut(s) 247, 655
BstMWI GCNNNNNNNGC 3 cut(s) 188, 263, 712
BstNSI RCATGY 1 cut(s) 706
BstSFI CTRYAG 1 cut(s) 561
BstV1I GCAGC 4 cut(s) 169, 367, 389, 699
BstX2I RGATCY 2 cut(s) 247, 655
BstYI RGATCY 2 cut(s) 247, 655
BsuRI GGCC 3 cut(s) 294, 539, 631
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 1 cut(s) 649
Cac8I GCNNGC 1 cut(s) 168
CfoI GCGC 1 cut(s) 830
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 2 cut(s) 293, 542
Csp6I GTAC 1 cut(s) 847
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 2 cut(s) 633, 703
CviQI GTAC 1 cut(s) 847
DdeI CTNAG 2 cut(s) 770, 791
DpnI GATC 2 cut(s) 249, 657
DpnII GATC 2 cut(s) 247, 655
DraI TTTAAA 1 cut(s) 696
EaeI YGGCCR 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 855
Eco47I GGWCC 1 cut(s) 542
Eco47III AGCGCT 1 cut(s) 829
Eco53kI GAGCTC 1 cut(s) 853
EcoICRI GAGCTC 1 cut(s) 853
EcoT38I GRGCYC 1 cut(s) 855
FaeI CATG 2 cut(s) 636, 706
FaqI GGGAC 2 cut(s) 53, 581
FatI CATG 2 cut(s) 632, 702
FauI CCCGC 1 cut(s) 48
Fnu4HI GCNGC 5 cut(s) 183, 292, 378, 381, 713
FokI GGATG 1 cut(s) 656
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 5 cut(s) 183, 292, 378, 381, 713
FspBI CTAG 2 cut(s) 437, 825
GlaI GCGC 1 cut(s) 829
GluI GCNGC 5 cut(s) 183, 292, 378, 381, 713
HaeII RGCGCY 1 cut(s) 831
HaeIII GGCC 3 cut(s) 294, 539, 631
HapII CCGG 2 cut(s) 533, 540
HhaI GCGC 1 cut(s) 830
Hin1II CATG 2 cut(s) 636, 706
Hin6I GCGC 1 cut(s) 828
HinP1I GCGC 1 cut(s) 828
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 5 cut(s) 49, 144, 689, 750, 793
HpaII CCGG 2 cut(s) 533, 540
HphI GGTGA 2 cut(s) 37, 196
Hpy166II GTNNAC 2 cut(s) 220, 666
Hpy188I TCNGA 2 cut(s) 114, 429
Hpy188III TCNNGA 2 cut(s) 320, 797
Hpy8I GTNNAC 2 cut(s) 220, 666
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 2 cut(s) 229, 776
HpyCH4III ACNGT 4 cut(s) 433, 520, 562, 670
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 8 cut(s) 85, 123, 191, 377, 494, 647, 706, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 263, 712
HpyF3I CTNAG 2 cut(s) 770, 791
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 2 cut(s) 636, 706
HspAI GCGC 1 cut(s) 828
Kzo9I GATC 2 cut(s) 247, 655
LmnI GCTCC 2 cut(s) 4, 779
Lsp1109I GCAGC 4 cut(s) 169, 367, 389, 699
LweI GCATC 2 cut(s) 113, 634
MaeI CTAG 2 cut(s) 437, 825
MaeII ACGT 1 cut(s) 42
MalI GATC 2 cut(s) 249, 657
MboI GATC 2 cut(s) 247, 655
MboII GAAGA 5 cut(s) 40, 43, 266, 566, 796
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 2 cut(s) 247, 655
MhlI GDGCHC 2 cut(s) 484, 855
MluCI AATT 3 cut(s) 131, 360, 707
MlyI GAGTC 3 cut(s) 138, 744, 802
MmeI TCCRAC 3 cut(s) 334, 430, 452
MnlI CCTC 6 cut(s) 16, 134, 316, 319, 576, 740
MseI TTAA 3 cut(s) 71, 695, 810
MslI CAYNNNNRTG 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 380
MspCI CTTAAG 1 cut(s) 70
MspI CCGG 2 cut(s) 533, 540
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 257
MwoI GCNNNNNNNGC 3 cut(s) 188, 263, 712
NdeII GATC 2 cut(s) 247, 655
NlaIII CATG 2 cut(s) 636, 706
NlaIV GGNNCC 1 cut(s) 426
NspI RCATGY 1 cut(s) 706
PctI GAATGC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 49, 689
PflMI CCANNNNNTGG 1 cut(s) 638
PkrI GCNGC 5 cut(s) 184, 293, 379, 382, 714
PleI GAGTC 3 cut(s) 138, 744, 801
PpsI GAGTC 3 cut(s) 138, 744, 801
Psp124BI GAGCTC 1 cut(s) 855
PspN4I GGNNCC 1 cut(s) 426
PspPI GGNCC 2 cut(s) 293, 542
PsuI RGATCY 2 cut(s) 247, 655
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 1 cut(s) 848
RsaNI GTAC 1 cut(s) 847
RseI CAYNNNNRTG 1 cut(s) 578
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 3 cut(s) 71, 695, 810
SatI GCNGC 5 cut(s) 183, 292, 378, 381, 713
Sau3AI GATC 2 cut(s) 247, 655
Sau96I GGNCC 2 cut(s) 293, 542
SchI GAGTC 3 cut(s) 138, 744, 802
SduI GDGCHC 2 cut(s) 484, 855
SfaNI GCATC 2 cut(s) 113, 634
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 578
SmlI CTYRAG 2 cut(s) 70, 320
SmoI CTYRAG 2 cut(s) 70, 320
Sse9I AATT 3 cut(s) 131, 360, 707
SsiI CCGC 2 cut(s) 55, 291
SspMI CTAG 2 cut(s) 437, 825
SstI GAGCTC 1 cut(s) 855
TaaI ACNGT 4 cut(s) 433, 520, 562, 670
TaiI ACGT 1 cut(s) 45
TaqI TCGA 1 cut(s) 796
TaqII GACCGA 1 cut(s) 798
TasI AATT 3 cut(s) 131, 360, 707
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 2 cut(s) 49, 689
Tru1I TTAA 3 cut(s) 71, 695, 810
Tru9I TTAA 3 cut(s) 71, 695, 810
TseI GCWGC 4 cut(s) 182, 377, 380, 712
TspDTI ATGAA 2 cut(s) 398, 567
Van91I CCANNNNNTGG 1 cut(s) 638
Vha464I CTTAAG 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 542
XceI RCATGY 1 cut(s) 706
XspI CTAG 2 cut(s) 437, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.