Rmu_sc0008085.1_g000020

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008085.1
OG0023035
Physical Location & Seq
Reverse (-)
73528 .. 74052
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008085.1_g000020.1.cds

Sequence Viewer

Length: 525 bp
atgcttgttcaaaacaagttcttggttctaatagtactggtctttgttgttggaacaatcaagtttggggagcgaacttgtgctctgtatcttgcaagtctagaccattttggggagactgtattgcccaagttggttgttggacctgactatgaagaaactgtagcaatccacacttcaatgaggtcagtcgaagtcccaacaacacaagtagtggtgccaatcaggccaaatattgggaatgcatataaagatccccagtttacagttgagggaaatctcgaagaatctttaaatgacatgcaattgctgcaagaagcgtactacctctttgaaagcttcaaaggactcattattggcttctttctcagctccaaaaacccgaaactgagtcgatatttcttccttaaaagaacctatactagagcttttcagttgatagggtacgaacttagtttcatgtatgaggttcttcacaccaaggttgttgtagtgcgcaaaattgggtgcacctctacttggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

174

Amino Acids

19.91

Weight (kDa)

7.74

Isoelectric Point (pI)

23.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023035)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000288.1_g000034 Rmu_sc0008085.1_g000020 Rmu_sc0009296.1_g000016
rosa_rugosa Rorug03G0157200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 236
AclWI GGATC 1 cut(s) 248
AfaI GTAC 3 cut(s) 36, 323, 446
AfiI CCNNNNNNNGG 3 cut(s) 112, 236, 519
AgsI TTSAA 4 cut(s) 11, 180, 335, 343
AluBI AGCT 3 cut(s) 339, 372, 428
AluI AGCT 3 cut(s) 339, 372, 428
Alw21I GWGCWC 2 cut(s) 85, 512
Alw26I GTCTC 1 cut(s) 110
Alw44I GTGCAC 1 cut(s) 508
AlwI GGATC 1 cut(s) 248
AoxI GGCC 1 cut(s) 227
ApaLI GTGCAC 1 cut(s) 508
ApeKI GCWGC 1 cut(s) 310
AspLEI GCGC 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 143
AvaII GGWCC 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 512
BanI GGYRCC 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 85, 512
BbvI GCAGC 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 2 cut(s) 101, 423
BfmI CTRYAG 1 cut(s) 162
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 219
BmrI ACTGGG 1 cut(s) 253
BmuI ACTGGG 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 480
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 236, 519
Bse1I ACTGG 2 cut(s) 42, 259
BseDI CCNNGG 1 cut(s) 480
BseLI CCNNNNNNNGG 3 cut(s) 112, 236, 519
BseMII CTCAG 2 cut(s) 380, 382
BseNI ACTGG 2 cut(s) 42, 259
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 1 cut(s) 297
BshFI GGCC 1 cut(s) 229
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 85, 512
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 3 cut(s) 112, 236, 519
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 85, 512
Bsp143I GATC 1 cut(s) 253
BspANI GGCC 1 cut(s) 229
BspCNI CTCAG 2 cut(s) 381, 381
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 217
BsrI ACTGG 2 cut(s) 42, 259
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 480
Bst4CI ACNGT 3 cut(s) 121, 163, 268
BstAPI GCANNNNNTGC 1 cut(s) 310
BstDEI CTNAG 3 cut(s) 368, 389, 452
BstHHI GCGC 1 cut(s) 498
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 2 cut(s) 226, 310
BstNSI RCATGY 1 cut(s) 304
BstSFI CTRYAG 1 cut(s) 162
BstSLI GKGCMC 1 cut(s) 512
BstV1I GCAGC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 229
CfoI GCGC 1 cut(s) 498
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 3 cut(s) 35, 322, 445
CviAII CATG 2 cut(s) 301, 460
CviJI RGCY 5 cut(s) 229, 339, 360, 372, 428
CviKI_1 RGCY 5 cut(s) 229, 339, 360, 372, 428
CviQI GTAC 3 cut(s) 35, 322, 445
DdeI CTNAG 3 cut(s) 368, 389, 452
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
DraI TTTAAA 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 480
EcoT22I ATGCAT 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 2 cut(s) 304, 463
FaiI YATR 7 cut(s) 153, 247, 249, 302, 420, 461, 465
FaqI GGGAC 1 cut(s) 182
FatI CATG 2 cut(s) 300, 459
Fnu4HI GCNGC 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 2 cut(s) 101, 423
FspI TGCGCA 1 cut(s) 497
GlaI GCGC 1 cut(s) 497
GluI GCNGC 1 cut(s) 311
HaeIII GGCC 1 cut(s) 229
HhaI GCGC 1 cut(s) 498
Hin1II CATG 2 cut(s) 304, 463
Hin6I GCGC 1 cut(s) 496
HinP1I GCGC 1 cut(s) 496
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 3 cut(s) 287, 348, 391
Hpy166II GTNNAC 2 cut(s) 264, 510
Hpy188III TCNNGA 2 cut(s) 101, 281
Hpy8I GTNNAC 2 cut(s) 264, 510
HpyCH4III ACNGT 3 cut(s) 121, 163, 268
HpyCH4V TGCA 5 cut(s) 95, 245, 304, 313, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 310
HpyF3I CTNAG 3 cut(s) 368, 389, 452
Hsp92II CATG 2 cut(s) 304, 463
HspAI GCGC 1 cut(s) 496
Kzo9I GATC 1 cut(s) 253
LmnI GCTCC 2 cut(s) 70, 377
LpnPI CCDG 4 cut(s) 23, 159, 211, 272
Lsp1109I GCAGC 1 cut(s) 297
MaeI CTAG 2 cut(s) 101, 423
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 4 cut(s) 167, 296, 394, 464
MfeI CAATTG 1 cut(s) 305
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 2 cut(s) 85, 512
MluCI AATT 2 cut(s) 305, 501
MlyI GAGTC 2 cut(s) 342, 400
MmeI TCCRAC 2 cut(s) 31, 121
MnlI CCTC 5 cut(s) 177, 265, 338, 460, 523
Mph1103I ATGCAT 1 cut(s) 247
MseI TTAA 2 cut(s) 293, 408
MslI CAYNNNNRTG 1 cut(s) 179
MunI CAATTG 1 cut(s) 305
Mva1269I GAATGC 1 cut(s) 247
MwoI GCNNNNNNNGC 2 cut(s) 226, 310
NdeII GATC 1 cut(s) 253
NlaIII CATG 2 cut(s) 304, 463
NlaIV GGNNCC 1 cut(s) 219
NsbI TGCGCA 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 1 cut(s) 304
PctI GAATGC 1 cut(s) 247
PfeI GAWTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 236
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 2 cut(s) 342, 399
PpsI GAGTC 2 cut(s) 342, 399
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 143
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 3 cut(s) 36, 323, 446
RsaNI GTAC 3 cut(s) 35, 322, 445
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 2 cut(s) 293, 408
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 1 cut(s) 143
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 2 cut(s) 342, 400
SduI GDGCHC 2 cut(s) 85, 512
SfcI CTRYAG 1 cut(s) 162
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 2 cut(s) 305, 501
SspI AATATT 1 cut(s) 235
SspMI CTAG 2 cut(s) 101, 423
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 3 cut(s) 121, 163, 268
TaqI TCGA 3 cut(s) 192, 282, 394
TasI AATT 2 cut(s) 305, 501
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 2 cut(s) 293, 408
Tru9I TTAA 2 cut(s) 293, 408
TseI GCWGC 1 cut(s) 310
TspDTI ATGAA 2 cut(s) 168, 448
Van91I CCANNNNNTGG 1 cut(s) 236
VneI GTGCAC 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 143
XbaI TCTAGA 1 cut(s) 100
XceI RCATGY 1 cut(s) 304
XspI CTAG 2 cut(s) 101, 423
ZrmI AGTACT 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.