Rorug03G0157200

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0023035
Physical Location & Seq
Forward (+)
12933314 .. 12935182
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0157200.1

Sequence Viewer

Length: 1023 bp
ATGAAGCTACCCGCGAAGCCCATATTGAGTCCGGGTCGGAGCGACAAGTTCCCGCCGCCGTTGATGAGGTTTCTGAGGAGCAATGTCGGGAGCAAAAGCCGAGGTCGGTTACGTGCGAGTCCGATGTTCGTGAGGAAGAAGAACACGACCGCCGTCGAAACACAAGAGCCGTCGTCGCCGAAAGTGACCTGCATGGGCCAGGTTCGGGTCAGGCGGTCGTCCTCCGCGGCTTCTTCGCGACGCGGACGGGGAAGAAGTGCCGGAGCTCCGACCCGGAAACGGTTCGGGTGGATCCAAAACGCCGTGTTCTGCAGCGATTTTCCCGGGAAAATCAAGCCCAAGTCTTGCAGACACCTCTGGCGGAAATGGGTTCCGTTTTTCCAAGTTGGGTTTTGCACCAGAGTTCAAATCGAAAAAGACCCACCAAAAGTTGAGACCCAATTTGGGGAAGCAATTGATGGGTTTTGTTCAGGTGATGATGAAGAAGAAGAAGAAGAACACGAAGAACCAGAATTTGAAAGAAAAGCCAAGGCTTTTGCTGCTTCTAGCTTTTCGCCACCCAAGAACGCTTTGCTCTTAACTCGATGCAGATCTGCGCCGTACAGGTCGTCTTCTTTGGCTAGTAGATTTTGGGGCTCGCCAGACAGAGCTAAAGAAGAAACAGGGGAAGAAGAACAGAGCAAAACAGGGGAAGCAGAACAGAGCATCGAGCCCCAAAACAGAGAAAATGGCAGCGAAAATAAGAGACCCACATCGGAAAGAGAGTCCATTTCGGAACAAGAATCGAGAACTGAGCAACAAAATAAAGAAAAGATGGAAATTTTCAAGGGATTGGAAAACTCAATCAGAGAAAGAATGGCCAAATCTGCGAGCATTGAAGATTCGAATGAAGGGGAGTTTGGATCAGACTCTGCTCGGCCTATGATTCTCACAAGGTGTAAGTCAGAACCAGCAAGAACAGCAGCAGAGAAGCTCGACCCAGATTCGTTGATTCATTCCCACCAAGTGTTTGTGATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

340

Amino Acids

38.32

Weight (kDa)

9.28

Isoelectric Point (pI)

72.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0023035)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000288.1_g000034 Rmu_sc0008085.1_g000020 Rmu_sc0009296.1_g000016
rosa_rugosa Rorug03G0157200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
AccII CGCG 4 cut(s) 14, 227, 238, 243
AciI CCGC 9 cut(s) 12, 53, 56, 150, 214, 225, 227, 243, 361
AclWI GGATC 3 cut(s) 286, 299, 910
AcoI YGGCCR 1 cut(s) 858
AcsI RAATTY 2 cut(s) 512, 819
AdeI CACNNNGTG 2 cut(s) 936, 1006
AfaI GTAC 1 cut(s) 602
AfiI CCNNNNNNNGG 4 cut(s) 205, 279, 444, 445
AgsI TTSAA 4 cut(s) 407, 518, 826, 878
AjnI CCWGG 1 cut(s) 198
AjuI GAANNNNNNNTTGG 2 cut(s) 882, 914
AluBI AGCT 5 cut(s) 7, 266, 549, 650, 973
AluI AGCT 5 cut(s) 7, 266, 549, 650, 973
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 2 cut(s) 428, 739
AlwI GGATC 3 cut(s) 286, 299, 910
AlwNI CAGNNNCTG 1 cut(s) 911
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 3 cut(s) 196, 858, 917
ApeKI GCWGC 4 cut(s) 312, 539, 732, 962
ApoI RAATTY 2 cut(s) 512, 819
AseI ATTAAT 1 cut(s) 1017
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 598
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 4 cut(s) 33, 274, 324, 325
AsuHPI GGTGA 1 cut(s) 485
AsuII TTCGAA 1 cut(s) 884
AvaI CYCGRG 1 cut(s) 323
BalI TGGCCA 1 cut(s) 860
BamHI GGATCC 1 cut(s) 291
BanII GRGCYC 3 cut(s) 268, 638, 714
BbsI GAAGAC 1 cut(s) 603
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 4 cut(s) 324, 526, 744, 974
BccI CCATC 2 cut(s) 452, 808
BceAI ACGGC 5 cut(s) 43, 137, 154, 287, 583
BciT130I CCWGG 1 cut(s) 200
BcnI CCSGG 4 cut(s) 33, 274, 324, 325
BcoDI GTCTC 2 cut(s) 428, 739
BfaI CTAG 2 cut(s) 546, 621
BfmI CTRYAG 1 cut(s) 310
BfuAI ACCTGC 1 cut(s) 197
BglII AGATCT 1 cut(s) 590
BisI GCNGC 6 cut(s) 56, 228, 313, 540, 733, 963
BlsI GCNGC 6 cut(s) 57, 229, 314, 541, 734, 964
Bme1390I CCNGG 5 cut(s) 33, 200, 274, 324, 325
BmeT110I CYCGRG 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 2 cut(s) 293, 372
BmrFI CCNGG 5 cut(s) 33, 200, 274, 324, 325
BmsI GCATC 2 cut(s) 575, 714
BoxI GACNNNNGTC 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 603
Bpu14I TTCGAA 1 cut(s) 884
BpuMI CCSGG 4 cut(s) 33, 274, 324, 325
BsaAI YACGTR 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 589
BsaI GGTCTC 2 cut(s) 428, 739
BsaJI CCNNGG 4 cut(s) 100, 225, 323, 528
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 279, 444, 445
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 589
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 4 cut(s) 100, 225, 323, 528
BseJI GATNNNNATC 1 cut(s) 589
BseLI CCNNNNNNNGG 4 cut(s) 205, 279, 444, 445
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 65, 783
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 4 cut(s) 324, 526, 744, 974
Bsh1236I CGCG 4 cut(s) 14, 227, 238, 243
Bsh1285I CGRYCG 2 cut(s) 150, 218
BshFI GGCC 3 cut(s) 198, 860, 919
BsiEI CGRYCG 2 cut(s) 150, 218
BsiHKAI GWGCWC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 4 cut(s) 32, 261, 274, 324
BslI CCNNNNNNNGG 4 cut(s) 205, 279, 444, 445
BsmAI GTCTC 2 cut(s) 428, 739
BsnI GGCC 3 cut(s) 198, 860, 919
Bso31I GGTCTC 2 cut(s) 428, 739
BsoBI CYCGRG 1 cut(s) 323
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 3 cut(s) 268, 638, 714
Bsp143I GATC 3 cut(s) 291, 590, 902
Bsp68I TCGCGA 1 cut(s) 238
BspACI CCGC 9 cut(s) 12, 53, 56, 150, 214, 225, 227, 243, 361
BspANI GGCC 3 cut(s) 198, 860, 919
BspCNI CTCAG 2 cut(s) 66, 784
BspFNI CGCG 4 cut(s) 14, 227, 238, 243
BspLI GGNNCC 2 cut(s) 293, 372
BspMAI CTGCAG 1 cut(s) 314
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 3 cut(s) 286, 299, 910
BspT104I TTCGAA 1 cut(s) 884
BspTNI GGTCTC 2 cut(s) 428, 739
BsrDI GCAATG 1 cut(s) 88
BssECI CCNNGG 4 cut(s) 100, 225, 323, 528
BssMI GATC 3 cut(s) 291, 590, 902
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 884
BstC8I GCNNGC 2 cut(s) 638, 871
BstDEI CTNAG 2 cut(s) 74, 792
BstDSI CCRYGG 1 cut(s) 225
BstFNI CGCG 4 cut(s) 14, 227, 238, 243
BstHHI GCGC 1 cut(s) 598
BstKTI GATC 3 cut(s) 294, 593, 905
BstMAI GTCTC 2 cut(s) 428, 739
BstMBI GATC 3 cut(s) 291, 590, 902
BstMCI CGRYCG 2 cut(s) 150, 218
BstMWI GCNNNNNNNGC 4 cut(s) 175, 539, 866, 959
BstNI CCWGG 1 cut(s) 200
BstPAI GACNNNNGTC 1 cut(s) 152
BstSCI CCNGG 5 cut(s) 31, 198, 272, 322, 323
BstSFI CTRYAG 1 cut(s) 310
BstUI CGCG 4 cut(s) 14, 227, 238, 243
BstV1I GCAGC 4 cut(s) 324, 526, 744, 974
BstV2I GAAGAC 1 cut(s) 603
BstX2I RGATCY 2 cut(s) 291, 590
BstYI RGATCY 2 cut(s) 291, 590
BsuRI GGCC 3 cut(s) 198, 860, 919
BtgI CCRYGG 1 cut(s) 225
BtuMI TCGCGA 1 cut(s) 238
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 2 cut(s) 638, 871
CaiI CAGNNNCTG 1 cut(s) 911
CfoI GCGC 1 cut(s) 598
Cfr13I GGNCC 1 cut(s) 196
Cfr42I CCGCGG 1 cut(s) 228
Cfr9I CCCGGG 1 cut(s) 323
CseI GACGC 1 cut(s) 249
Csp6I GTAC 1 cut(s) 601
CviAII CATG 1 cut(s) 193
CviQI GTAC 1 cut(s) 601
DdeI CTNAG 2 cut(s) 74, 792
DpnI GATC 3 cut(s) 293, 592, 904
DpnII GATC 3 cut(s) 291, 590, 902
DraIII CACNNNGTG 2 cut(s) 936, 1006
EaeI YGGCCR 1 cut(s) 858
EciI GGCGGA 1 cut(s) 376
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 3 cut(s) 268, 638, 714
Eco31I GGTCTC 2 cut(s) 428, 739
Eco53kI GAGCTC 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 323
EcoICRI GAGCTC 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 3 cut(s) 268, 638, 714
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 1 cut(s) 196
FaiI YATR 3 cut(s) 23, 194, 923
FatI CATG 1 cut(s) 192
FauI CCCGC 2 cut(s) 19, 60
Fnu4HI GCNGC 6 cut(s) 56, 228, 313, 540, 733, 963
FriOI GRGCYC 3 cut(s) 268, 638, 714
Fsp4HI GCNGC 6 cut(s) 56, 228, 313, 540, 733, 963
FspBI CTAG 2 cut(s) 546, 621
GlaI GCGC 1 cut(s) 597
GluI GCNGC 6 cut(s) 56, 228, 313, 540, 733, 963
HaeIII GGCC 3 cut(s) 198, 860, 919
HapII CCGG 4 cut(s) 32, 261, 274, 324
HgaI GACGC 1 cut(s) 249
HhaI GCGC 1 cut(s) 598
Hin1II CATG 1 cut(s) 196
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HinfI GANTC 9 cut(s) 28, 118, 764, 782, 881, 908, 925, 983, 991
HpaII CCGG 4 cut(s) 32, 261, 274, 324
HphI GGTGA 1 cut(s) 485
Hpy188I TCNGA 9 cut(s) 39, 75, 123, 270, 757, 775, 848, 907, 946
Hpy188III TCNNGA 4 cut(s) 88, 130, 237, 786
Hpy99I CGWCG 4 cut(s) 158, 175, 178, 243
HpyAV CCTTC 1 cut(s) 884
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 5 cut(s) 192, 312, 348, 396, 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 539, 866, 959
HpyF3I CTNAG 2 cut(s) 74, 792
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 1 cut(s) 196
HspAI GCGC 1 cut(s) 596
KspI CCGCGG 1 cut(s) 228
Kzo9I GATC 3 cut(s) 291, 590, 902
LmnI GCTCC 5 cut(s) 39, 78, 90, 263, 271
Lsp1109I GCAGC 4 cut(s) 324, 526, 744, 974
LweI GCATC 2 cut(s) 575, 714
MaeI CTAG 2 cut(s) 546, 621
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 2 cut(s) 108, 184
MalI GATC 3 cut(s) 293, 592, 904
MboI GATC 3 cut(s) 291, 590, 902
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 2 cut(s) 291, 590
MhlI GDGCHC 3 cut(s) 268, 638, 714
MlsI TGGCCA 1 cut(s) 860
MluCI AATT 5 cut(s) 440, 453, 512, 819, 1018
MluNI TGGCCA 1 cut(s) 860
MlyI GAGTC 4 cut(s) 37, 127, 773, 902
MmeI TCCRAC 2 cut(s) 17, 293
MnlI CCTC 6 cut(s) 60, 69, 95, 126, 232, 365
Mox20I TGGCCA 1 cut(s) 860
MroXI GAANNNNTTC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 860
MseI TTAA 2 cut(s) 578, 1017
Msp20I TGGCCA 1 cut(s) 860
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 4 cut(s) 32, 261, 274, 324
MspR9I CCNGG 5 cut(s) 33, 200, 274, 324, 325
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 4 cut(s) 14, 227, 238, 243
MwoI GCNNNNNNNGC 4 cut(s) 175, 539, 866, 959
NciI CCSGG 4 cut(s) 33, 274, 324, 325
NdeII GATC 3 cut(s) 291, 590, 902
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 2 cut(s) 293, 372
NmeAIII GCCGAG 2 cut(s) 125, 895
NmuCI GTSAC 1 cut(s) 184
NruI TCGCGA 1 cut(s) 238
NspV TTCGAA 1 cut(s) 884
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 5 cut(s) 782, 881, 925, 983, 991
PkrI GCNGC 6 cut(s) 57, 229, 314, 541, 734, 964
PleI GAGTC 4 cut(s) 36, 126, 772, 902
PpsI GAGTC 4 cut(s) 36, 126, 772, 902
Ppu21I YACGTR 1 cut(s) 113
PshAI GACNNNNGTC 1 cut(s) 152
PshBI ATTAAT 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 293, 372
PspPI GGNCC 1 cut(s) 196
PstI CTGCAG 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 911
PsuI RGATCY 2 cut(s) 291, 590
RruI TCGCGA 1 cut(s) 238
RsaI GTAC 1 cut(s) 602
RsaNI GTAC 1 cut(s) 601
SacI GAGCTC 1 cut(s) 268
SacII CCGCGG 1 cut(s) 228
SaqAI TTAA 2 cut(s) 578, 1017
SatI GCNGC 6 cut(s) 56, 228, 313, 540, 733, 963
Sau3AI GATC 3 cut(s) 291, 590, 902
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 4 cut(s) 37, 127, 773, 902
ScrFI CCNGG 5 cut(s) 33, 200, 274, 324, 325
SduI GDGCHC 3 cut(s) 268, 638, 714
SfaNI GCATC 2 cut(s) 575, 714
SfcI CTRYAG 1 cut(s) 310
Sfr303I CCGCGG 1 cut(s) 228
SfuI TTCGAA 1 cut(s) 884
SgrBI CCGCGG 1 cut(s) 228
SmaI CCCGGG 1 cut(s) 325
Sse9I AATT 5 cut(s) 440, 453, 512, 819, 1018
SsiI CCGC 9 cut(s) 12, 53, 56, 150, 214, 225, 227, 243, 361
SspMI CTAG 2 cut(s) 546, 621
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 5 cut(s) 31, 198, 272, 322, 323
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 1 cut(s) 282
TaiI ACGT 1 cut(s) 115
TaqI TCGA 7 cut(s) 156, 411, 583, 708, 785, 884, 975
TasI AATT 5 cut(s) 440, 453, 512, 819, 1018
TauI GCSGC 2 cut(s) 58, 230
TfiI GAWTC 5 cut(s) 782, 881, 925, 983, 991
Tru1I TTAA 2 cut(s) 578, 1017
Tru9I TTAA 2 cut(s) 578, 1017
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 4 cut(s) 312, 539, 732, 962
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 4 cut(s) 17, 495, 903, 983
TspGWI ACGGA 1 cut(s) 363
TspMI CCCGGG 1 cut(s) 323
VspI ATTAAT 1 cut(s) 1017
XapI RAATTY 2 cut(s) 512, 819
XmaI CCCGGG 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 2 cut(s) 546, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.