Rmu_sc0000429.1_g000058

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000429.1
OG0021465
Physical Location & Seq
Reverse (-)
253669 .. 255666
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1800 bp
atgtcaagtcttcttgcagctgaaaacgatgccaaggaaatcgagcgctacatacccttctctaactttgtgataaatggtgattgggagaatgcaaaggagtgtcttacaaaacttccagatgcaataacagcaagataccaatttggagccacagcccttcatatagcagccgggcaagggcacctgcatatcgtgaaagagttggtcttgctgatgggacaagatgatttacaaactaaaaacgcttatggttacacagctcttcatgtagcattcttcaacgggcgctttgacattgttaaagagctgttgcccctgatgggaggagtaaaagactgccatggtaacacggttcttcacatggcattaattaggcagcaaccacacactgtagagtggatggtgaagttaatgaaaagagaagacttggaattaaaaaacaacgatggttctacagctctgaacatagcagtcaagaaggggaatgtgtctactattaaagagttgctgccgcatgatgatgactttgaccgttatataccgtttactaatgctgtaattgaccgtgattggaataatgcaaactattgtcttagagatctagatccctacagggcagtaagagcaagagatccaagagatggaaatgagaacacggctcttcacttagcaatcaagcatgggcatgtggatattgtgaaagagttggtgtcgttgatgacacaagaagatttggaagtgaaaaatgctgatccttctacgggtaatttaaatgcagacctcagtgaacgagcagtcgtcgaaatggctaagtacatggttgaacagaatgagatactgcttgcccgtgttttagaactagagaatgcttttggagatacacctattcacgaagcagtcagtaagggggatatgtctattgtggaagaattggtaccttcgatgagacaagaaggttttgaaataaaaaataataatggttttacagctcttcaccaagcagtcatgaacgaaaatgtggatattgtgaagattatagtccggcggatgaggcgagaaggtctggaagtaaaaggtgctcttggttacacagcacttcaccaagcagtgaagatggggaatatggggattgtgaaagagattgtattgttgatgagacaagaaggtttcgtagtaaaaaacgacgagggtttcaatgctcttcacctagcagtcatgaagggggatgtggatatcgtgaagatgttggtctcactgatgagacgagatgatttggaaataaaaggcgctcttggttacacagcacttcaccaagcagtgaagatggggaatatggggattgtgaaagagttgctattggtgatgagacaagagggtcttgaagtaaaaaatgacgcgggttccaatgctcttcaccttgcagtcatggagaataagaatatgggcattgcgagagagttgatgtcgttgatgagaccgcaagcgatggaaataaaaaatggtcaaggttttacagctttaggttgtgctatggagcttggcgccaaagatggagacttttgggaaatagctaagtacatggccggaaagaacaaaaatgtatttggcattcgctcgggtagattcattccagttgtatgggctgctgcctggtgcaaatggaaattaactcactgtctctattccgtcactccacgtgaagttctaaacgggtttaacggcgctcagttaattacttttgctattgaacgtctgcaagcgcttggtaagagtgttttaaatatcaattattattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

599

Amino Acids

67.0

Weight (kDa)

6.09

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 42 - 105 6.1e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 54 - 102 6.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 111 - 178 6.7e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 287 - 348 9.8e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 361 - 419 6.7e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 367 - 453 3.7e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 367 - 418 2.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 437 - 517 7.5e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021465)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0242411 RchiOBHm_Chr7g0242471
rosa_multiflora Rmu_sc0000429.1_g000058 Rmu_sc0000429.1_g000066
rosa_rugosa Rorug04G0006800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 195
AasI GACNNNNNNGTC 1 cut(s) 1377
Acc36I ACCTGC 1 cut(s) 195
Acc65I GGTACC 1 cut(s) 937
AccB1I GGYRCC 3 cut(s) 183, 937, 1544
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 1400
AciI CCGC 4 cut(s) 515, 1048, 1400, 1481
AclWI GGATC 3 cut(s) 602, 629, 749
AcoI YGGCCR 1 cut(s) 1584
AcvI CACGTG 1 cut(s) 1700
AcyI GRCGYC 1 cut(s) 1545
AfaI GTAC 3 cut(s) 818, 939, 1580
AfeI AGCGCT 2 cut(s) 47, 1764
AfiI CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
AgsI TTSAA 6 cut(s) 283, 827, 965, 1198, 1385, 1751
AjnI CCWGG 1 cut(s) 1652
AluBI AGCT 8 cut(s) 20, 263, 310, 461, 992, 1520, 1540, 1574
AluI AGCT 8 cut(s) 20, 263, 310, 461, 992, 1520, 1540, 1574
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
AlwI GGATC 3 cut(s) 602, 629, 749
Ama87I CYCGRG 1 cut(s) 1618
Aor51HI AGCGCT 2 cut(s) 47, 1764
AoxI GGCC 1 cut(s) 1584
ApeKI GCWGC 6 cut(s) 17, 170, 379, 511, 1646, 1649
AseI ATTAAT 1 cut(s) 371
Asp718I GGTACC 1 cut(s) 937
AspLEI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 8 cut(s) 92, 418, 989, 1094, 1199, 1304, 1375, 1409
AvaI CYCGRG 1 cut(s) 1618
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 3 cut(s) 183, 937, 1544
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BbrPI CACGTG 1 cut(s) 1700
BbsI GAAGAC 2 cut(s) 2, 432
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
BccI CCATC 9 cut(s) 211, 316, 397, 443, 638, 1111, 1321, 1483, 1547
BceAI ACGGC 2 cut(s) 675, 1738
BciT130I CCWGG 1 cut(s) 1654
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
BfaI CTAG 3 cut(s) 605, 863, 1211
BfmI CTRYAG 3 cut(s) 393, 456, 613
BfoI RGCGCY 6 cut(s) 49, 292, 1293, 1548, 1728, 1766
BfuAI ACCTGC 1 cut(s) 195
BglII AGATCT 1 cut(s) 601
BisI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
BlsI GCNGC 7 cut(s) 19, 172, 381, 513, 516, 1648, 1651
Bme1390I CCNGG 2 cut(s) 175, 1654
BmeT110I CYCGRG 1 cut(s) 1618
BmiI GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
BmrFI CCNGG 2 cut(s) 175, 1654
BmsI GCATC 2 cut(s) 19, 112
BpiI GAAGAC 2 cut(s) 2, 432
BpuMI CCSGG 1 cut(s) 175
BsaAI YACGTR 1 cut(s) 1700
BsaBI GATNNNNATC 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 1545
BsaI GGTCTC 2 cut(s) 1258, 1471
BsaJI CCNNGG 2 cut(s) 33, 343
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
Bse1I ACTGG 1 cut(s) 1634
Bse3DI GCAATG 1 cut(s) 1449
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 1 cut(s) 1654
BseDI CCNNGG 2 cut(s) 33, 343
BseGI GGATG 3 cut(s) 408, 1056, 1234
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
BseMI GCAATG 1 cut(s) 1449
BseMII CTCAG 2 cut(s) 799, 1742
BseNI ACTGG 1 cut(s) 1634
BseRI GAGGAG 1 cut(s) 342
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
Bsh1236I CGCG 1 cut(s) 1400
BshFI GGCC 1 cut(s) 1586
BshNI GGYRCC 3 cut(s) 183, 937, 1544
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 1618
BsiSI CCGG 3 cut(s) 174, 1045, 1587
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 4 cut(s) 180, 323, 644, 764
BsmAI GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
BsmBI CGTCTC 1 cut(s) 1258
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 4 cut(s) 97, 275, 874, 1611
BsnI GGCC 1 cut(s) 1586
Bso31I GGTCTC 2 cut(s) 1258, 1471
BsoBI CYCGRG 1 cut(s) 1618
Bsp1286I GDGCHC 2 cut(s) 186, 1084
Bsp143I GATC 4 cut(s) 601, 607, 634, 754
Bsp19I CCATGG 1 cut(s) 343
BspACI CCGC 4 cut(s) 515, 1048, 1400, 1481
BspANI GGCC 1 cut(s) 1586
BspCNI CTCAG 2 cut(s) 798, 1741
BspFNI CGCG 1 cut(s) 1400
BspHI TCATGA 2 cut(s) 1008, 1218
BspLI GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
BspMI ACCTGC 1 cut(s) 195
BspPI GGATC 3 cut(s) 602, 629, 749
BspQI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
BspT107I GGYRCC 3 cut(s) 183, 937, 1544
BspTNI GGTCTC 2 cut(s) 1258, 1471
BsrDI GCAATG 1 cut(s) 1449
BsrI ACTGG 1 cut(s) 1634
BssECI CCNNGG 2 cut(s) 33, 343
BssMI GATC 4 cut(s) 601, 607, 634, 754
BssNI GRCGYC 1 cut(s) 1545
BssT1I CCWWGG 2 cut(s) 33, 343
Bst2UI CCWGG 1 cut(s) 1654
Bst4CI ACNGT 6 cut(s) 355, 394, 536, 546, 569, 1679
Bst6I CTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
BstACI GRCGYC 1 cut(s) 1545
BstBAI YACGTR 1 cut(s) 1700
BstC8I GCNNGC 3 cut(s) 846, 1485, 1761
BstDEI CTNAG 6 cut(s) 596, 670, 785, 813, 1575, 1728
BstDSI CCRYGG 1 cut(s) 343
BstF5I GGATG 3 cut(s) 408, 1056, 1234
BstFNI CGCG 1 cut(s) 1400
BstH2I RGCGCY 6 cut(s) 49, 292, 1293, 1548, 1728, 1766
BstHHI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
BstKTI GATC 4 cut(s) 604, 610, 637, 757
BstMAI GTCTC 8 cut(s) 943, 1153, 1258, 1258, 1363, 1471, 1551, 1685
BstMBI GATC 4 cut(s) 601, 607, 634, 754
BstMWI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
BstNI CCWGG 1 cut(s) 1654
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 2 cut(s) 173, 1652
BstSFI CTRYAG 3 cut(s) 393, 456, 613
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 1 cut(s) 1400
BstV1I GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
BstV2I GAAGAC 2 cut(s) 2, 432
BstX2I RGATCY 3 cut(s) 601, 607, 634
BstXI CCANNNNNNTGG 1 cut(s) 1641
BstYI RGATCY 3 cut(s) 601, 607, 634
BsuRI GGCC 1 cut(s) 1586
BtgI CCRYGG 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 1502
BtsCI GGATG 3 cut(s) 408, 1056, 1234
BtsI GCAGTG 2 cut(s) 1116, 1326
BtsIMutI CAGTG 6 cut(s) 390, 793, 1116, 1256, 1326, 1675
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 3 cut(s) 846, 1485, 1761
CciI TCATGA 2 cut(s) 1008, 1218
CfoI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
CseI GACGC 1 cut(s) 1406
Csp6I GTAC 3 cut(s) 817, 938, 1579
CviQI GTAC 3 cut(s) 817, 938, 1579
DdeI CTNAG 6 cut(s) 596, 670, 785, 813, 1575, 1728
DinI GGCGCC 1 cut(s) 1546
DpnI GATC 4 cut(s) 603, 609, 636, 756
DpnII GATC 4 cut(s) 601, 607, 634, 754
DraI TTTAAA 2 cut(s) 774, 1782
DrdI GACNNNNNNGTC 1 cut(s) 1377
DseDI GACNNNNNNGTC 1 cut(s) 1377
EaeI YGGCCR 1 cut(s) 1584
Eam1104I CTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
EarI CTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
EciI GGCGGA 1 cut(s) 1063
Eco130I CCWWGG 2 cut(s) 33, 343
Eco31I GGTCTC 2 cut(s) 1258, 1471
Eco32I GATATC 1 cut(s) 1237
Eco47III AGCGCT 2 cut(s) 47, 1764
Eco72I CACGTG 1 cut(s) 1700
Eco88I CYCGRG 1 cut(s) 1618
EcoRII CCWGG 1 cut(s) 1652
EcoRV GATATC 1 cut(s) 1237
EcoT14I CCWWGG 2 cut(s) 33, 343
EgeI GGCGCC 1 cut(s) 1546
EheI GGCGCC 1 cut(s) 1546
ErhI CCWWGG 2 cut(s) 33, 343
Esp3I CGTCTC 1 cut(s) 1258
FalI AAGNNNNNCTT 6 cut(s) 1068, 1100, 1278, 1310, 1365, 1397
FaqI GGGAC 1 cut(s) 234
FauI CCCGC 1 cut(s) 1393
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
FokI GGATG 3 cut(s) 415, 1063, 1241
Fsp4HI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
FspBI CTAG 3 cut(s) 605, 863, 1211
GlaI GCGC 6 cut(s) 47, 290, 1291, 1546, 1726, 1764
GluI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
HaeII RGCGCY 6 cut(s) 49, 292, 1293, 1548, 1728, 1766
HaeIII GGCC 1 cut(s) 1586
HapII CCGG 3 cut(s) 174, 1045, 1587
HgaI GACGC 1 cut(s) 1406
HhaI GCGC 6 cut(s) 48, 291, 1292, 1547, 1727, 1765
Hin1I GRCGYC 1 cut(s) 1545
Hin6I GCGC 6 cut(s) 46, 289, 1290, 1545, 1725, 1763
HinP1I GCGC 6 cut(s) 46, 289, 1290, 1545, 1725, 1763
HinfI GANTC 1 cut(s) 1626
HpaII CCGG 3 cut(s) 174, 1045, 1587
HphI GGTGA 8 cut(s) 92, 418, 989, 1094, 1199, 1304, 1375, 1409
Hpy166II GTNNAC 3 cut(s) 495, 549, 791
Hpy188I TCNGA 1 cut(s) 465
Hpy8I GTNNAC 3 cut(s) 495, 549, 791
Hpy99I CGWCG 2 cut(s) 806, 1190
HpyAV CCTTC 9 cut(s) 67, 170, 475, 768, 950, 951, 1055, 1160, 1216
HpyCH4III ACNGT 6 cut(s) 355, 394, 536, 546, 569, 1679
HpyCH4IV ACGT 2 cut(s) 1699, 1753
HpyCH4V TGCA 9 cut(s) 17, 95, 125, 190, 584, 779, 1424, 1659, 1759
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
HpyF3I CTNAG 6 cut(s) 596, 670, 785, 813, 1575, 1728
HpySE526I ACGT 2 cut(s) 1699, 1753
Hsp92I GRCGYC 1 cut(s) 1545
HspAI GCGC 6 cut(s) 46, 289, 1290, 1545, 1725, 1763
KasI GGCGCC 1 cut(s) 1544
KpnI GGTACC 1 cut(s) 941
Kzo9I GATC 4 cut(s) 601, 607, 634, 754
LguI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
LmnI GCTCC 2 cut(s) 149, 1537
Lsp1109I GCAGC 6 cut(s) 29, 182, 391, 498, 1633, 1636
LweI GCATC 2 cut(s) 19, 112
MaeI CTAG 3 cut(s) 605, 863, 1211
MaeII ACGT 2 cut(s) 1699, 1753
MaeIII GTNAC 5 cut(s) 254, 347, 1088, 1298, 1690
MalI GATC 4 cut(s) 603, 609, 636, 756
MboI GATC 4 cut(s) 601, 607, 634, 754
MflI RGATCY 3 cut(s) 601, 607, 634
MhlI GDGCHC 2 cut(s) 186, 1084
MluCI AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1667, 1734, 1789
Mly113I GGCGCC 1 cut(s) 1545
MnlI CCTC 5 cut(s) 320, 794, 1047, 1183, 1369
MslI CAYNNNNRTG 2 cut(s) 522, 687
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 3 cut(s) 174, 1045, 1587
MspR9I CCNGG 2 cut(s) 175, 1654
Mva1269I GAATGC 4 cut(s) 97, 275, 874, 1611
MvaI CCWGG 1 cut(s) 1654
MvnI CGCG 1 cut(s) 1400
MwoI GCNNNNNNNGC 3 cut(s) 131, 626, 1054
NarI GGCGCC 1 cut(s) 1545
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 343
NdeII GATC 4 cut(s) 601, 607, 634, 754
NlaIV GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
NmuCI GTSAC 1 cut(s) 1690
NspI RCATGY 1 cut(s) 692
PagI TCATGA 2 cut(s) 1008, 1218
PaqCI CACCTGC 1 cut(s) 195
PciSI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
PctI GAATGC 4 cut(s) 97, 275, 874, 1611
PfeI GAWTC 1 cut(s) 1626
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 7 cut(s) 19, 172, 381, 513, 516, 1648, 1651
PluTI GGCGCC 1 cut(s) 1548
PmaCI CACGTG 1 cut(s) 1700
PmlI CACGTG 1 cut(s) 1700
Ppu21I YACGTR 1 cut(s) 1700
PshBI ATTAAT 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 1652
PspCI CACGTG 1 cut(s) 1700
PspGI CCWGG 1 cut(s) 1652
PspN4I GGNNCC 5 cut(s) 151, 185, 939, 1405, 1546
PsuI RGATCY 3 cut(s) 601, 607, 634
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 818, 939, 1580
RsaNI GTAC 3 cut(s) 817, 938, 1579
RseI CAYNNNNRTG 2 cut(s) 522, 687
SapI GCTCTTC 5 cut(s) 270, 669, 999, 1209, 1419
SatI GCNGC 7 cut(s) 18, 171, 380, 512, 515, 1647, 1650
Sau3AI GATC 4 cut(s) 601, 607, 634, 754
ScrFI CCNGG 2 cut(s) 175, 1654
SduI GDGCHC 2 cut(s) 186, 1084
SfaNI GCATC 2 cut(s) 19, 112
SfcI CTRYAG 3 cut(s) 393, 456, 613
SfoI GGCGCC 1 cut(s) 1546
SmiI ATTTAAAT 1 cut(s) 774
SmiMI CAYNNNNRTG 2 cut(s) 522, 687
Sse9I AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1667, 1734, 1789
SsiI CCGC 4 cut(s) 515, 1048, 1400, 1481
SspDI GGCGCC 1 cut(s) 1544
SspMI CTAG 3 cut(s) 605, 863, 1211
StyD4I CCNGG 2 cut(s) 173, 1652
StyI CCWWGG 2 cut(s) 33, 343
SwaI ATTTAAAT 1 cut(s) 774
TaaI ACNGT 6 cut(s) 355, 394, 536, 546, 569, 1679
TaiI ACGT 2 cut(s) 1702, 1756
TaqI TCGA 3 cut(s) 42, 804, 944
TasI AATT 9 cut(s) 143, 372, 434, 561, 769, 932, 1667, 1734, 1789
TatI WGTACW 2 cut(s) 816, 1578
TauI GCSGC 1 cut(s) 517
TfiI GAWTC 1 cut(s) 1626
TscAI CASTG 6 cut(s) 397, 793, 1116, 1263, 1326, 1682
TseFI GTSAC 1 cut(s) 1690
TseI GCWGC 6 cut(s) 17, 170, 379, 511, 1646, 1649
Tsp45I GTSAC 1 cut(s) 1690
TspDTI ATGAA 6 cut(s) 152, 257, 431, 1025, 1235, 1618
TspGWI ACGGA 1 cut(s) 1678
TspRI CASTG 6 cut(s) 397, 793, 1116, 1263, 1326, 1682
Van91I CCANNNNNTGG 1 cut(s) 644
VspI ATTAAT 1 cut(s) 371
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 3 cut(s) 605, 863, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.